Abstract:
:Nonbleachable rhodopsins containing retinal moieties with fixed 11-ene structures have been prepared. When the nonbleachable rhodopsin analogue corresponding to the natural pigment was flash-photolysed at 20.8 degrees C, no absorption changes occurred at the monitoring wavelengths of 380, 480, and 580 nm for the time range of 2 microseconds--10 s. This observation is in contrast to that of natural rhodopsin which showed the formation of metarhodopsin I and its decay to meta II. Irradiation of the artificial rhodopsin, 77 K, with light of 460 and 540 nm, also gave no spectral changes; in the case of natural rhodopsin, however, the irradiation leads to formation of the red-shifted intermediate bathorhodopsin. The absence of photochemistry in the artificial pigment shows that an 11-cis to trans photoisomerization of the retinal moiety is a crucial step in inducing the chain of events in te photolysis of rhodopsin.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Mao B,Tsuda M,Ebrey TG,Akita H,Balogh-Nair V,Nakanishi Kdoi
10.1016/S0006-3495(81)84809-6subject
Has Abstractpub_date
1981-08-01 00:00:00pages
543-6issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(81)84809-6journal_volume
35pub_type
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