Repetitive propagation of action potentials destabilizes the structure of the myelin sheath. A dynamic x-ray diffraction study.

abstract：:We have formulated a kinetic model for the primary steps that occur at the cell membrane during receptor-mediated endocytosis. This model includes the diffusion of receptor molecules, the binding of receptors to coated pits, the loss of coated pits by invagination, and random reinsertion of receptors and coated pits. ...

journal_title：Biophysical journal

authors： Keizer J,Ramirez J,Peacock-López E

更新日期：1985-01-01 00:00:00

abstract：:A pulsed source neutron spectrometer has been used to measure vibrational spectra (20-4000 cm-1) of dry and hydrated type I collagen fibers, and of two model polypeptides, polyproline II and (prolyl-prolyl-glycine)10, at temperatures of 30 and 120 K. the collagen spectra provide the first high resolution neutron views...

journal_title：Biophysical journal

authors： Middendorf HD,Hayward RL,Parker SF,Bradshaw J,Miller A

更新日期：1995-08-01 00:00:00

abstract：:Cell spreading is involved in many physiological and pathological processes. The spreading behavior of a cell significantly depends on its microenvironment, but the biochemomechanical mechanisms of geometry-confined cell spreading remain unclear. A dynamic model is here established to investigate the spreading of cell...

journal_title：Biophysical journal

authors： Zhao ZL,Liu ZY,Du J,Xu GK,Feng XQ

更新日期：2017-06-06 00:00:00

abstract：:Crossover motifs are integral components for designing DNA-based nanostructures and nanomechanical devices due to their enhanced rigidity compared to the normal B-DNA. Although the structural rigidity of the double helix B-DNA has been investigated extensively using both experimental and theoretical tools, to date the...

journal_title：Biophysical journal

authors： Santosh M,Maiti PK

更新日期：2011-09-21 00:00:00

abstract：:Steady-state and time-resolved fluorescence spectroscopy was used to follow the local and global changes in structure and dynamics during chemical and thermal denaturation of unlabeled human serum albumin (HSA) and HSA with an acrylodan moiety bound to Cys34. Acrylodan fluorescence was monitored to obtain information ...

journal_title：Biophysical journal

authors： Flora K,Brennan JD,Baker GA,Doody MA,Bright FV

更新日期：1998-08-01 00:00:00

abstract：:Structure factor amplitudes and phases can be computed directly from electron cryomicroscopy images. Inherent aberrations of the electromagnetic lenses and other instrumental factors affect the structure factors, however, resulting in decreased accuracy in the determined three-dimensional reconstruction. In contrast, ...

journal_title：Biophysical journal

authors： Thuman-Commike PA,Tsuruta H,Greene B,Prevelige PE Jr,King J,Chiu W

更新日期：1999-04-01 00:00:00

abstract：:The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference...

journal_title：Biophysical journal

authors： Zhang ZW,Neu B

更新日期：2009-08-19 00:00:00

abstract：:In skeletal muscle, excitation-contraction coupling involves the activation of dihydropyridine receptors (DHPR) and type-1 ryanodine receptors (RyR1) to produce depolarization-dependent sarcoplasmic reticulum Ca²⁺ release via orthograde signaling. Another form of DHPR-RyR1 communication is retrograde signaling, in whi...

journal_title：Biophysical journal

authors： Olojo RO,Hernández-Ochoa EO,Ikemoto N,Schneider MF

更新日期：2011-05-18 00:00:00

abstract：:Chiral objects which scatter and absorb preferentially left versus right circularly polarized light give rise to bright-field circular dichroism (CD) images containing contributions from both these two phenomena. These contributions are separated and characterized mathematically, and the effect of the dimensions of th...

journal_title：Biophysical journal

authors： Finzi L,Ulibarri L,Bustamante C

更新日期：1991-06-01 00:00:00

abstract：:The geometry and mechanical properties of the human erythrocyte membrane cytoskeleton are investigated by a computer simulation in which the cytoskeleton is represented by a network of polymer chains. Four elastic moduli as well as the area and thickness are predicted for the chain network as a function of temperature...

journal_title：Biophysical journal

authors： Boal DH

更新日期：1994-08-01 00:00:00

abstract：:Phase-resolved monitoring of photoacoustic signals can provide information about the depth profile of a sample. We describe an application of this principle to determine the depth profiles of ligands and antitumor agents in mammalian cells. Measurements of the in-phase and quadrature components of the photoacoustic sp...

journal_title：Biophysical journal

authors： Narayanan K,Chandani S,Ramakrishna T,Rao CM

更新日期：1997-05-01 00:00:00

abstract：:Based on experimental evidence for 2D array formation of bacteriorhodopsin, we propose a general model for lipid-mediated 2D array formation of membrane proteins in lipid bilayers. The model includes two different lipid species, "annular" lipids and "neutral" lipids, and one protein species. The central assumption of ...

journal_title：Biophysical journal

authors： Sabra MC,Uitdehaag JC,Watts A

更新日期：1998-09-01 00:00:00

abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

journal_title：Biophysical journal

authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

更新日期：1993-09-01 00:00:00

abstract：:The dependences of the steady-state critical concentration and average filament length of actin solutions, on the filament branching and capping rates, are calculated using a rate methodology based on the total number of actin filaments. The methodology generalizes calculations of the "treadmilling" actin concentratio...

journal_title：Biophysical journal

authors： Carlsson AE

更新日期：2005-07-01 00:00:00

abstract：:A simulation of the function of the human heart and heart muscle has been developed in the form of a digital computer code. For a given set of values for the input variables, realistic values of the cardiac output variables are predicted. A detailed discussion of the simulation and some results obtained from its appli...

journal_title：Biophysical journal

authors： Hanna WT

更新日期：1973-07-01 00:00:00

abstract：:Using the molecular dynamics simulation technique, we studied the changes occurring in a dipalmitoylphosphatidylcholine (DPPC):cholesterol (CH) membrane at 50 mol% sterol when cholesterol is replaced with cholesterol sulfate (CS). Our simulations were performed at constant pressure and temperature on a nanosecond time...

journal_title：Biophysical journal

authors： Smondyrev AM,Berkowitz ML

更新日期：2000-04-01 00:00:00

abstract：:Many animals regulate power generation for locomotion by varying the number of muscle fibers used for movement. However, insects with asynchronous flight muscles may regulate the power required for flight by varying the calcium concentration ([Ca(2+)]). In vivo myoplasmic calcium levels in Drosophila flight muscle hav...

journal_title：Biophysical journal

authors： Wang Q,Zhao C,Swank DM

更新日期：2011-11-02 00:00:00

abstract：:Several biologically important protein structures give rise to strong second-harmonic generation (SHG) in their native context. In addition to high-contrast optical sections of cells and tissues, SHG imaging can provide detailed structural information based on the physical constraints of the optical effect. In this st...

journal_title：Biophysical journal

authors： Plotnikov SV,Millard AC,Campagnola PJ,Mohler WA

更新日期：2006-01-15 00:00:00

abstract：:Glycerinated muscle fibers isolated from rabbit psoas muscle, and a number of other nonmuscle elastic fibers including glass, rubber, and collagen, were exposed to hydrostatic pressures of up to 10 MPa (100 Atm) to determine the pressure sensitivity of their isometric tension. The isometric tension of muscle fibers in...

journal_title：Biophysical journal

authors： Ranatunga KW,Fortune NS,Geeves MA

更新日期：1990-12-01 00:00:00

abstract：:The influence of mass transport on ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor, was investigated. A one-dimensional computer model for the mass transport of ligand between the bulk solution and the polymer gel and within the gel was employed, and the influe...

journal_title：Biophysical journal

authors： Schuck P

更新日期：1996-03-01 00:00:00

abstract：:Nitrile hydratase (NHase) is an enzyme used in the industrial biotechnological production of acrylamide. The active site, which contains nonheme iron or noncorrin cobalt, is buried in the protein core at the interface of two domains, alpha and beta. Hydrogen bonds between betaArg-56 and alphaCys-114 sulfenic acid (alp...

journal_title：Biophysical journal

authors： Kubiak K,Nowak W

更新日期：2008-05-15 00:00:00

abstract：:A long-standing question about membrane structure and function is the degree to which the physical properties of the inner and outer leaflets of a bilayer are coupled to one another. Using our recently developed methods to prepare asymmetric vesicles, coupling was investigated for vesicles containing phosphatidylcholi...

journal_title：Biophysical journal

authors： Chiantia S,London E

更新日期：2012-12-05 00:00:00

abstract：:Hair cells of the inner ear can power spontaneous oscillations of their mechanosensory hair bundle, resulting in amplification of weak inputs near the characteristic frequency of oscillation. Recently, dynamic force measurements have revealed that delayed gating of the mechanosensitive ion channels responsible for mec...

journal_title：Biophysical journal

authors： Barral J,Jülicher F,Martin P

更新日期：2018-01-23 00:00:00

abstract：:The role of desolvation in protein binding kinetics is investigated using Brownian dynamics simulations in complexes in which the electrostatic interactions are relatively weak. We find that partial desolvation, modeled by a short-range atomic contact potential, is not only a major contributor to the binding free ener...

journal_title：Biophysical journal

authors： Camacho CJ,Kimura SR,DeLisi C,Vajda S

更新日期：2000-03-01 00:00:00

abstract：:Myelin basic protein (MBP) is predominantly found in the membranes of the myelin sheath of the central nervous system and is involved in important protein-protein and protein-lipid interactions in vivo and in vitro. Furthermore, divalent transition metal ions, especially Zn(2+) and Cu(2+), seem to directly affect the ...

journal_title：Biophysical journal

authors： Bund T,Boggs JM,Harauz G,Hellmann N,Hinderberger D

更新日期：2010-11-03 00:00:00

abstract：:Cellular membranes are laterally organized into domains of distinct structures and compositions by the differential interaction affinities between various membrane lipids and proteins. A prominent example of such structures are lipid rafts, which are ordered, tightly packed domains that have been widely implicated in ...

journal_title：Biophysical journal

authors： Lin X,Gorfe AA,Levental I

更新日期：2018-04-24 00:00:00

abstract：:Immobilization is a key step involved in probing molecular interactions using single-molecule force spectroscopy methods, including atomic force microscopy (AFM). To our knowledge, we describe a novel approach termed flexible nanoarray (FNA) in which the interaction between the two internally immobilized amyloid β pep...

journal_title：Biophysical journal

authors： Krasnoslobodtsev AV,Zhang Y,Viazovkina E,Gall A,Bertagni C,Lyubchenko YL

更新日期：2015-05-05 00:00:00

abstract：:We study the folding thermodynamics and kinetics of the Pin1 WW domain, a three-stranded beta-sheet protein, by using all-atom (except nonpolar hydrogens) discontinuous molecular dynamics simulations at various temperatures with a Gō model. The protein exhibits a two-state folding kinetics near the folding transition ...

journal_title：Biophysical journal

authors： Luo Z,Ding J,Zhou Y

更新日期：2007-09-15 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:We developed a model of the rat ventricular myocyte at room temperature to predict the relative effects of different mechanisms on the cause of the slow increase in force in response to a step change in muscle length. We performed simulations in the presence of stretch-dependent increases in flux through the Na(+)-H(+...

journal_title：Biophysical journal

authors： Niederer SA,Smith NP

更新日期：2007-06-01 00:00:00