Molecular mechanism of lateral diffusion of py(10)-PC and free pyrene in fluid DMPC bilayers.

abstract：:Biological membranes composed of lipids and proteins are in contact with electrolytes like aqueous NaCl solutions. Based on molecular dynamics studies it is widely believed that Na(+) ions specifically bind to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes, whereas Cl(-) ions stay in solution. Here,...

journal_title：Biophysical journal

authors： Knecht V,Klasczyk B

更新日期：2013-02-19 00:00:00

abstract：:A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...

journal_title：Biophysical journal

authors： Paillotin G,Geacintov NE,Breton J

更新日期：1983-10-01 00:00:00

abstract：:Characterizing the ionic distribution around chromatin is important for understanding the electrostatic forces governing chromatin structure and function. Here we develop an electrostatic model to handle multivalent ions and compute the ionic distribution around a mesoscale chromatin model as a function of conformatio...

journal_title：Biophysical journal

authors： Gan HH,Schlick T

更新日期：2010-10-20 00:00:00

abstract：:Laser Raman spectroscopy has been used detect structural changes in troponin C induced by Ca2+ binding. Addition of Ca2+ - Mg2+ sites produces perturbations in the amide III region of the spectrum indicative of increased alpha-helical content, and in regions of the spectrum corresponding to carboxylate, thiol, and phe...

journal_title：Biophysical journal

authors： Carew EB,Leavis PC,Stanley HE,Gergely J

更新日期：1980-05-01 00:00:00

abstract：:Cell adhesion to extracellular matrix is mediated by receptor-ligand interactions. When a cell first contacts a surface, it spreads, exerting traction forces against the surface and forming new bonds as its contact area expands. Here, we examined the changes in shape, actin polymerization, focal adhesion formation, an...

journal_title：Biophysical journal

authors： Reinhart-King CA,Dembo M,Hammer DA

更新日期：2005-07-01 00:00:00

abstract：:The interaction of an amphiphilic, 40-amino acid beta-amyloid (Abeta) peptide with liposomal membranes as a function of sterol mole fraction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane sterol probe, dehydroergosterol (DHE), and fluorescence resonance energy transfer (FRET) ...

journal_title：Biophysical journal

authors： Qiu L,Lewis A,Como J,Vaughn MW,Huang J,Somerharju P,Virtanen J,Cheng KH

更新日期：2009-05-20 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is generally used to obtain information about the number of fluorescent particles in a small volume and the diffusion coefficient from the autocorrelation function of the fluorescence signal. Here we demonstrate that photon counting histogram (PCH) analysis constitutes a nov...

journal_title：Biophysical journal

authors： Chen Y,Müller JD,So PT,Gratton E

更新日期：1999-07-01 00:00:00

abstract：:Dielectrophoresis and electrorotation are commonly used to measure dielectric properties and membrane electrical parameters of biological cells. We have derived quantitative relationships for several critical points, defined in Fig. A 1, which characterize the dielectrophoretic spectrum and the electrorotational spect...

journal_title：Biophysical journal

authors： Gimsa J,Marszalek P,Loewe U,Tsong TY

更新日期：1991-10-01 00:00:00

abstract：:Lateral organization of cholesterol in dioleoyl-phosphatidylcholine (DOPC) lipid bilayers at high cholesterol concentration (>45 mol%) was investigated using steady-state fluorescence anisotropy and fluorescent resonance energy transfer techniques. The recently devised Low Temperature Trap method was used to prepare c...

journal_title：Biophysical journal

authors： Parker A,Miles K,Cheng KH,Huang J

更新日期：2004-03-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a central focal adhesion protein that promotes focal adhesion turnover, but the role of FAK for cell mechanical stability is unknown. We measured the mechanical properties of wild-type (FAKwt), FAK-deficient (FAK-/-), FAK-silenced (siFAK), and siControl mouse embryonic fibroblasts by mag...

journal_title：Biophysical journal

authors： Fabry B,Klemm AH,Kienle S,Schäffer TE,Goldmann WH

更新日期：2011-11-02 00:00:00

abstract：:Nuclear calcium is a key signal in the dialogue between synapse and nucleus that controls the genomic responses required for persistent adaptations, including memory and acquired neuroprotection. The amplitude and duration of nuclear calcium transients specify activity-induced transcriptional changes. However, the pre...

journal_title：Biophysical journal

authors： Bengtson CP,Freitag HE,Weislogel JM,Bading H

更新日期：2010-12-15 00:00:00

abstract：:Anionic lipids are key components in the cell membranes. Many cell-regulatory and signaling mechanisms depend upon a complicated interplay between them and membrane-bound proteins. Phospholipid bilayers are commonly used as model systems in experimental or theoretical studies to gain insight into the structure and dyn...

journal_title：Biophysical journal

authors： Broemstrup T,Reuter N

更新日期：2010-08-04 00:00:00

abstract：:Green flagellates possess rhodopsin-like photoreceptors involved in control of their behavior via generation of photocurrents across the plasma membrane. Chlamydomonas mutants blocked in retinal biosynthesis are "blind," but they can be rescued by the addition of exogenous retinoids. Photosignaling by chlamyrhodopsin ...

journal_title：Biophysical journal

authors： Govorunova EG,Sineshchekov OA,Gärtner W,Chunaev AS,Hegemann P

更新日期：2001-11-01 00:00:00

abstract：:The mechanisms by which extracellular electric field stimuli induce the (re)excitation of cardiac cells in various stages of refractoriness are still not well understood. We modeled the interactions between an isolated cardiac cell and imposed extracellular electric fields to determine the mechanisms by which relative...

journal_title：Biophysical journal

authors： Fishler MG,Sobie EA,Thakor NV,Tung L

更新日期：1996-03-01 00:00:00

abstract：:Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interr...

journal_title：Biophysical journal

authors： Otto MR,Lillo MP,Beechem JM

更新日期：1994-12-01 00:00:00

abstract：:The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...

journal_title：Biophysical journal

authors： Dembo M,Oliver T,Ishihara A,Jacobson K

更新日期：1996-04-01 00:00:00

abstract：:In addition to the annular or boundary lipids that surround the transmembrane surface of the potassium channel KcsA from Streptomyces lividans, x-ray crystallographic studies have detected one anionic lipid molecule bound at each protein-protein interface in the homotetrameric structure, at sites referred to as nonann...

journal_title：Biophysical journal

authors： Marius P,Zagnoni M,Sandison ME,East JM,Morgan H,Lee AG

更新日期：2008-03-01 00:00:00

abstract：:In wild-type lenses from various species, an intracellular hydrostatic pressure gradient goes from ∼340 mmHg in central fiber cells to 0 mmHg in surface cells. This gradient drives a center-to-surface flow of intracellular fluid. In lenses in which gap-junction coupling is increased, the central pressure is lower, whe...

journal_title：Biophysical journal

authors： Gao J,Sun X,White TW,Delamere NA,Mathias RT

更新日期：2015-11-03 00:00:00

abstract：:Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...

journal_title：Biophysical journal

authors： Zhou J,Peluffo RD

更新日期：2010-11-17 00:00:00

abstract：:Large-conductance Ca(2+)-activated K(+) channels (BK) play a fundamental role in modulating membrane potential in many cell types. The gating of BK channels and its modulation by Ca(2+) and voltage has been the subject of intensive research over almost three decades, yielding several of the most complicated kinetic me...

journal_title：Biophysical journal

authors： Rosales RA,Varanda WA

更新日期：2009-05-20 00:00:00

abstract：:Using the simple argument based on irreversible thermodynamics and the Gouy-Chapman theory of the double layer, we show that the equilibrium distribution of charged lipid molecules between the two surfaces of a bilayer is asymmetric if the two solutions bathing the surfaces have the same ionic strength but contain ion...

journal_title：Biophysical journal

authors： McQuarrie DA,Mulás P

更新日期：1977-02-01 00:00:00

abstract：:The rate of exchange of urea across the membranes of human erythrocytes (red blood cells) was quantified on the 1-s to 2-min timescale. (13)C-urea was hyperpolarized and subjected to rapid dissolution and the previously reported (partial) resolution of (13)C NMR resonances from the molecules inside and outside red blo...

journal_title：Biophysical journal

authors： Pagès G,Puckeridge M,Liangfeng G,Tan YL,Jacob C,Garland M,Kuchel PW

更新日期：2013-11-05 00:00:00

abstract：:We have measured the fluorescence spectra of a single fluorophore attached to a single protein molecule in aqueous solution using a total internal reflection fluorescence microscope. The most reactive cysteine residue of myosin subfragment-1 (S1) was labeled with tetramethylrhodamine. The spectral shift induced by a c...

journal_title：Biophysical journal

authors： Wazawa T,Ishii Y,Funatsu T,Yanagida T

更新日期：2000-03-01 00:00:00

abstract：:Proton translocation activity of bacteriorhodopsin mutants lacking the proton acceptor Asp-85 was investigated using the black lipid membrane technique. Mutants D85N, D85T, and D85,96N were constructed and homologously expressed in Halobacterium salinarium to yield a membrane fraction with a buoyant density of 1.18 g/...

journal_title：Biophysical journal

authors： Tittor J,Schweiger U,Oesterhelt D,Bamberg E

更新日期：1994-10-01 00:00:00

abstract：:There is no methodology for the estimation of the dynamic features of large-molecular-weight RNAs in homogeneous physiological media. In this report, a luminescence anisotropy-based method using a long-lifetime luminescent oligonucleotide probe for the estimation of the dynamic features of large-molecular-weight RNA i...

journal_title：Biophysical journal

authors： Sakamoto T,Mahara A,Yamagata K,Iwase R,Yamaoka T,Murakami A

更新日期：2005-12-01 00:00:00

abstract：:The bile acid-sensitive ion channel is activated by amphiphilic substances such as bile acids or artificial detergents via membrane alterations; however, the mechanism of membrane sensitivity of the bile acid-sensitive ion channel is not known. It has also not been systematically investigated whether other members of ...

journal_title：Biophysical journal

authors： Schmidt A,Alsop RJ,Rimal R,Lenzig P,Joussen S,Gervasi NN,Khondker A,Gründer S,Rheinstädter MC,Wiemuth D

更新日期：2018-03-27 00:00:00

abstract：:Interactions between amyloid-β peptide (Aβ) and the cell membrane include interaction with membrane lipids and binding to membrane receptors, both of which are considered to be the toxicity mechanisms of Aβ. However, it is unclear whether both mechanisms lead to cytotoxicity. Thus, we aimed to analyze these two mechan...

journal_title：Biophysical journal

authors： Shi JM,Zhu L,Lan X,Zhao DW,He YJ,Sun ZQ,Wu D,Li HY

更新日期：2020-09-15 00:00:00

abstract：:We have determined directly the effects of the inhibitory peptide phospholamban (PLB) on the rotational dynamics of the calcium pump (Ca-ATPase) of cardiac sarcoplasmic reticulum (SR). This was accomplished by comparing mouse ventricular SR, which has PLB levels similar to those found in other mammals, with mouse atri...

journal_title：Biophysical journal

authors： Voss JC,Mahaney JE,Jones LR,Thomas DD

更新日期：1995-05-01 00:00:00

abstract：:In this article, optimization-based frameworks are introduced for elucidating the input-output structure of signaling networks and for pinpointing targeted disruptions leading to the silencing of undesirable outputs in therapeutic interventions. The frameworks are demonstrated on a large-scale reconstruction of a sign...

journal_title：Biophysical journal

authors： Dasika MS,Burgard A,Maranas CD

更新日期：2006-07-01 00:00:00

abstract：:Crystallographic studies of insulin-protamine complexes, such as neutral protamine Hagedorn (NPH) insulin, have been hampered by high crystal solvent content, small crystal dimensions, and extensive disorder in the protamine molecules. We report herein in situ tapping mode atomic force microscopy (TMAFM) studies of cr...

journal_title：Biophysical journal

authors： Yip CM,Brader ML,Frank BH,DeFelippis MR,Ward MD

更新日期：2000-01-01 00:00:00