Effects of bath resistance on action potentials in the squid giant axon: myocardial implications.

abstract：:Difference spectra between resting and excited nerve in the infrared region between 2000 and 1000 cm(-1) have been examined with a resolution of 0.5 cm(-1). Spectra were obtained with a modified Perkin-Elmer model 521 grating infrared spectrophotometer (Perkin-Elmer Corp., Instrument Div., Norwalk, Conn.), and the sig...

journal_title：Biophysical journal

authors： Sherebrin MH,MacClement BA,Franko AJ

更新日期：1972-08-01 00:00:00

abstract：:Determining the spatial relationship of individual proteins in dense assemblies remains a challenge for superresolution nanoscopy. The organization of aquaporin-4 (AQP4) into large plasma membrane assemblies provides an opportunity to image membrane-bound AQP4 antibodies (AQP4-IgG) and evaluate changes in their spatia...

journal_title：Biophysical journal

authors： Soltys JN,Meyer SA,Schumann H,Gibson EA,Restrepo D,Bennett JL

更新日期：2017-04-25 00:00:00

abstract：:Computational methods that predict three-dimensional structures from amino acid sequences have become increasingly accurate and have provided insights into structure-function relationships for proteins in the absence of structural data. However, the accuracy of computational structural models requires experimental app...

journal_title：Biophysical journal

authors： Tateyama M,Liu H,Yang AS,Cormier JW,Kass RS

更新日期：2004-03-01 00:00:00

abstract：:Quantitative characterization of protein interactions under physiological conditions is vital for systems biology. Fluorescence photobleaching/activation experiments of GFP-tagged proteins are frequently used for this purpose, but robust analysis methods to extract physicochemical parameters from such data are lacking...

journal_title：Biophysical journal

authors： Beaudouin J,Mora-Bermúdez F,Klee T,Daigle N,Ellenberg J

更新日期：2006-03-15 00:00:00

abstract：:The nuclear magnetic resonance (NMR) of water protons in live and glycerinated muscle, suspensions of glycerinated myofibrils, and solutions of several muscle proteins has been studied. T(1) and T(2), measured on partially hydrated proteins by pulsed spin-echo techniques, decreased as the ratio of water to protein dec...

journal_title：Biophysical journal

authors： Cooke R,Wien R

更新日期：1971-12-01 00:00:00

abstract：:A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...

journal_title：Biophysical journal

authors： Paillotin G,Geacintov NE,Breton J

更新日期：1983-10-01 00:00:00

abstract：:Two-dimensional electronic chirality-induced signals of excitons in the photosynthetic Fenna-Matthews-Olson complex from two species of green sulfur bacteria (Chlorobium tepidum and Prosthecochloris aestuarii) are compared. The spectra are predicted to provide sensitive probes of local protein environment of the const...

journal_title：Biophysical journal

authors： Voronine DV,Abramavicius D,Mukamel S

更新日期：2008-11-15 00:00:00

abstract：:Collagen fibrils form extracellular networks that regulate cell functions and provide mechanical strength to tissues. Collagen fibrillogenesis is an entropy-driven process promoted by warming and reversed by cooling. Here, we investigate the influence of noncovalent interactions mediated by the collagen triple helix o...

journal_title：Biophysical journal

authors： de Wild M,Pomp W,Koenderink GH

更新日期：2013-07-02 00:00:00

abstract：:The theoretical relationships between the fluorescence and photochemical yields of PS II and the fraction of open reaction centers are examined in a general model endowed with the following features: i) a homogeneous, infinite PS II domain; ii) exciton-radical-pair equilibrium; and iii) different rates of exciton tran...

journal_title：Biophysical journal

authors： Lavergne J,Trissl HW

更新日期：1995-06-01 00:00:00

abstract：:Hint2, one of the five members of the superfamily of the histidine triad AMP-lysine hydrolase proteins, is expressed in mitochondria of various cell types. In human adrenocarcinoma cells, Hint2 modulates Ca(2+) handling by mitochondria. As Hint2 is highly expressed in hepatocytes, we investigated if this protein affec...

journal_title：Biophysical journal

authors： Ndiaye D,Collado-Hilly M,Martin J,Prigent S,Dufour JF,Combettes L,Dupont G

更新日期：2013-09-03 00:00:00

abstract：:The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

journal_title：Biophysical journal

authors： Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

更新日期：2007-09-01 00:00:00

abstract：:Despite the importance of magnetic properties of biological samples for biomagnetism and related fields, the exact magnetic susceptibilities of most biological samples in their physiological conditions are still unknown. Here we used superconducting quantum interferometer device to detect the magnetic properties of no...

journal_title：Biophysical journal

authors： Tao Q,Zhang L,Han X,Chen H,Ji X,Zhang X

更新日期：2020-02-04 00:00:00

abstract：:Green flagellates possess rhodopsin-like photoreceptors involved in control of their behavior via generation of photocurrents across the plasma membrane. Chlamydomonas mutants blocked in retinal biosynthesis are "blind," but they can be rescued by the addition of exogenous retinoids. Photosignaling by chlamyrhodopsin ...

journal_title：Biophysical journal

authors： Govorunova EG,Sineshchekov OA,Gärtner W,Chunaev AS,Hegemann P

更新日期：2001-11-01 00:00:00

abstract：:Nuclear magnetic resonance (NMR) measurements provide both structural and dynamical information about the molecules in which nuclear resonances are observed. This manuscript addresses NMR relaxation of water protons in protein powder systems. Inclusion of magnetic communication between the water proton spins and prote...

journal_title：Biophysical journal

authors： Bryant RG,Shirley WM

更新日期：1980-10-01 00:00:00

abstract：:The protonation state and intracellular distribution of ellipticine were investigated in single human mammary T47D cells by confocal laser microspectrofluorimetry. In the cell nucleus, only the protonated form of ellipticine was detected as a direct consequence of its apparent pK increase upon DNA binding. Both proton...

journal_title：Biophysical journal

authors： Sureau F,Moreau F,Millot JM,Manfait M,Allard B,Aubard J,Schwaller MA

更新日期：1993-11-01 00:00:00

abstract：:Diverse microbial communities coordinate group behaviors through signal exchange, such as the exchange of acyl-homoserine lactones (AHLs) by Gram-negative bacteria. Cellular communication is prone to interference by neighboring microbes. One mechanism of interference is signal destruction through the production of an ...

journal_title：Biophysical journal

authors： Silva KP,Chellamuthu P,Boedicker JQ

更新日期：2017-03-14 00:00:00

abstract：:We present the measurement of the force required to rupture a single protein-sugar bond using a methodology that provides selective discrimination between specific and nonspecific binding events and helps verify the presence of a single functional molecule on the atomic force microscopy tip. In particular, the interac...

journal_title：Biophysical journal

authors： Ratto TV,Langry KC,Rudd RE,Balhorn RL,Allen MJ,McElfresh MW

更新日期：2004-04-01 00:00:00

abstract：:The AXH domain of protein Ataxin 1 is thought to play a key role in the misfolding and aggregation pathway responsible for Spinocerebellar ataxia 1. For this reason, a molecular level understanding of AXH oligomerization pathway is crucial to elucidate the aggregation mechanism, which is thought to trigger the disease...

journal_title：Biophysical journal

authors： Grasso G,Morbiducci U,Massai D,Tuszynski JA,Danani A,Deriu MA

更新日期：2018-01-23 00:00:00

abstract：:We propose a mechanism for the formation of membrane oscillations and traveling waves, which arise due to the coupling between the actin cytoskeleton and the calcium flux through the membrane. In our model, the fluid cell membrane has a mobile but constant population of proteins with a convex spontaneous curvature, wh...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2009-09-16 00:00:00

abstract：:This work builds upon the record-breaking speed and generous immediate release of new experimental 3D structures of the SARS-CoV-2 proteins and complexes, which are crucial to downstream vaccine and drug development. We have surveyed those structures to catch the occasional errors that could be significant for those i...

journal_title：Biophysical journal

authors： Croll TI,Williams CJ,Chen VB,Richardson DC,Richardson JS

更新日期：2021-01-15 00:00:00

abstract：:Structures of truncated versions of the influenza A virus M2 proton channel have been determined recently by x-ray crystallography in the open conformation of the channel, and by NMR in the closed state. The structures differ in the position of the bound inhibitors. The x-ray structure shows a single amantadine molecu...

journal_title：Biophysical journal

authors： Chuang GY,Kozakov D,Brenke R,Beglov D,Guarnieri F,Vajda S

更新日期：2009-11-18 00:00:00

abstract：:Based on the existence of ATP-sensitive potassium channels in the plasma membrane of pancreatic beta cells, we develop a quantitative explanation of the electrical activity observed in pancreatic islets. The proposed mechanism involves the voltage-dependent inward calcium and outward potassium currents described by Ro...

journal_title：Biophysical journal

authors： Keizer J,Magnus G

更新日期：1989-08-01 00:00:00

abstract：:Ubiquinol cytochrome c oxidoreductase (bc1 complex) serves as an important electron junction in many respiratory systems. It funnels electrons coming from NADH and ubiquinol to cytochrome c, but it is also capable of producing significant amounts of the free radical superoxide. In situ and in other experimental system...

journal_title：Biophysical journal

authors： Bazil JN

更新日期：2017-10-03 00:00:00

abstract：:The current-voltage (I-V) relation of the background current, IK1, was studied in isolated canine cardiac Purkinje myocytes using the whole-cell, patch-clamp technique. Since Ba2+ and Cs+ block IK1, these cations were used to separate the I-V relation of IK1 from that of the whole cell. The I-V relation of IK1 was mea...

journal_title：Biophysical journal

authors： Shah AK,Cohen IS,Datyner NB

更新日期：1987-10-01 00:00:00

abstract：:The kinetics of methyl-, ethyl-, iso-propyl-, and ter-butyl-isocyanide binding to Aplysia limacina myoglobin (distal His----Lys) and the isolated beta chains from hemoglobin Zurich (distal His----Arg) have been investigated by flash photolysis at various temperatures above 0 degrees C. Sperm whale (Physter catodon) my...

journal_title：Biophysical journal

authors： Di Iorio EE,Winterhalter KH,Giacometti GM

更新日期：1987-03-01 00:00:00

abstract：:The quantitation of human granulocyte movement using a stochastic differential equation is described. The method has the potential to distinguish both positive and negative chemotaxis. Analysis and information concerning cell movements can be obtained for any point in time and distance for the duration of the experime...

journal_title：Biophysical journal

authors： Boyarsky A,Noble PB,Peterson SC

更新日期：1976-03-01 00:00:00

abstract：:Gramicidin is a polypeptide antibiotic that forms monovalent cation-specific channels in membrane environments. In organic solvents and in lipids containing unsaturated fatty acid chains, it forms a double-helical "pore" structure, in which two monomers are intertwined. This form of gramicidin can bind two cations ins...

journal_title：Biophysical journal

authors： Chen Y,Wallace BA

更新日期：1996-07-01 00:00:00

abstract：:The mating pathway in Saccharomyces cerevisiae is one of the best understood signal transduction pathways in eukaryotes. It transmits the mating signal from plasma membrane into the nucleus through the G-protein coupled receptor and the mitogen-activated protein kinase (MAPK) cascade. According to current understandin...

journal_title：Biophysical journal

authors： Shao D,Zheng W,Qiu W,Ouyang Q,Tang C

更新日期：2006-12-01 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00

abstract：:We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described met...

journal_title：Biophysical journal

authors： Abreu MS,Moreno MJ,Vaz WL

更新日期：2004-07-01 00:00:00