Characterization of ion channels on the surface membrane of adult rat skeletal muscle.

abstract：:Recent experimental studies suggest that lactate dehydrogenase (LDH) binds its substrate via the formation of a LDH/NADH.substrate encounter complex through a select-fit mechanism, whereby only a minority population of LDH/NADH is binding-competent. In this study, we perform molecular dynamics calculations to explore ...

journal_title：Biophysical journal

authors： Pineda JR,Callender R,Schwartz SD

更新日期：2007-09-01 00:00:00

abstract：:Purple photosynthetic bacteria harvest light using pigment-protein complexes which are often arranged in pseudo-organelles called chromatophores. A model of a chromatophore from Rhodospirillum photometricum was constructed based on atomic force microscopy data. Molecular-dynamics simulations and quantum-dynamics calcu...

journal_title：Biophysical journal

authors： Chandler DE,Strümpfer J,Sener M,Scheuring S,Schulten K

更新日期：2014-06-03 00:00:00

abstract：:Sinusoidal oscillatory flow of blood and of aqueous glycerol solutions was produced in rigid cylindrical tubes. For aqueous glycerol, the amplitude of the measured pressure gradient wave form conformed closely to that predicted by Womersley's theory of oscillatory flow, up to Reynolds numbers approaching 2000. Blood d...

journal_title：Biophysical journal

authors： Kunz AL,Coulter NA Jr

更新日期：1967-01-01 00:00:00

abstract：:EXACT FORMULAS ARE DERIVED FOR THE ENERGY CHANGE OF A DIPOLE SYSTEM WITH TWO ENERGY STATES (OR BANDS) IN A CHANGING FIELD IN TWO CASES: (a) no dipole flip-flop and (b) dipole flip-flop caused by stimulation. Based on these formulas, the positive and negative heats are calculated. The results are in good agreement with...

journal_title：Biophysical journal

authors： Wei LY

更新日期：1972-09-01 00:00:00

abstract：:Interactions between proteins are often sufficiently weak that their study through the use of conventional structural techniques becomes problematic. Of the few techniques capable of providing experimental measures of weak protein-protein interactions, perhaps the most useful is the second virial coefficient, B(22), w...

journal_title：Biophysical journal

authors： Elcock AH,McCammon JA

更新日期：2001-02-01 00:00:00

abstract：:Mechanosensitivity in voltage-gated calcium channels could be an asset to calcium signaling in healthy cells or a liability during trauma. Recombinant N-type channels expressed in HEK cells revealed a spectrum of mechano-responses. When hydrostatic pressure inflated cells under whole-cell clamp, capacitance was unchan...

journal_title：Biophysical journal

authors： Calabrese B,Tabarean IV,Juranka P,Morris CE

更新日期：2002-11-01 00:00:00

abstract：:Probes and biosensors that incorporate luminescent Tb(III) or Eu(III) complexes are promising for cellular imaging because time-gated microscopes can detect their long-lifetime (approximately milliseconds) emission without interference from short-lifetime (approximately nanoseconds) fluorescence background. Moreover, ...

journal_title：Biophysical journal

authors： Rajendran M,Miller LW

更新日期：2015-07-21 00:00:00

abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

journal_title：Biophysical journal

authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

更新日期：1993-09-01 00:00:00

abstract：:Peptidoglycan hydrolases are bacterial secreted enzymes that cleave covalent bonds in the cell-wall peptidoglycan, thereby fulfilling major physiological functions during cell growth and division. Although the molecular structure and functional roles of these enzymes have been widely studied, the molecular details und...

journal_title：Biophysical journal

authors： Beaussart A,Rolain T,Duchêne MC,El-Kirat-Chatel S,Andre G,Hols P,Dufrêne YF

更新日期：2013-08-06 00:00:00

abstract：:During cytokinesis in Saccharomyces cerevisiae, damaged proteins are distributed unequally between the daughter and mother cells. The retention of these proteins is correlated with yeast aging. Even though evidence suggests that aggregates are retained due to an underlying molecular mechanism, the debate on whether an...

journal_title：Biophysical journal

authors： Andrade-Restrepo M

更新日期：2017-12-05 00:00:00

abstract：:Topical microbicide products are being developed for the prevention of sexually transmitted infections. These include vaginally-applied gels that deliver anti-HIV molecules. Gels may also provide partial barriers that slow virion diffusion from semen to vulnerable epithelium, increasing the time during which anti-HIV ...

journal_title：Biophysical journal

authors： Lai BE,Henderson MH,Peters JJ,Walmer DK,Katz DF

更新日期：2009-11-04 00:00:00

abstract：:From the study of the kinetics of the fluorescence self-quenching of pyrene in multilamellar vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in the fluid phase we obtain the molecular diffusion parameters, diffusion coefficients, and activation energies for lateral diffusion for three probes: 1-palmitoy...

journal_title：Biophysical journal

authors： Martins J,Melo E

更新日期：2001-02-01 00:00:00

abstract：:Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One limitation of cryopreservation is the low tolerance to freezing observed for oocytes and embryos rich in lipid droplets. We apply Raman spectroscopy to investigate freezing of lipid droplets inside cumulus-ooc...

journal_title：Biophysical journal

authors： Okotrub KA,Mokrousova VI,Amstislavsky SY,Surovtsev NV

更新日期：2018-08-07 00:00:00

abstract：:Measurement of the state of optical polarization of light diffracted from single, skinned and intact fibers of anterior tibialis muscle from Rana pipiens revealed a dependence upon rigor, activation, and sarcomere length (SL) change. Changes in total birefringence, delta nT, and differential field ratio value, rT, wer...

journal_title：Biophysical journal

authors： Chen JS,Baskin RJ,Baskin RJ,Burton K,Shen S,Yeh Y

更新日期：1989-09-01 00:00:00

abstract：:Neutron scattering and nuclear magnetic resonance relaxation experiments are combined with molecular dynamics (MD) simulations in a novel, to our knowledge, approach to investigate the change in internal dynamics on substrate (camphor) binding to a protein (cytochrome P450cam). The MD simulations agree well with both ...

journal_title：Biophysical journal

authors： Miao Y,Yi Z,Cantrell C,Glass DC,Baudry J,Jain N,Smith JC

更新日期：2012-11-21 00:00:00

abstract：:Using a method they developed, Stamatoff and Krimm (1976) have phased swelling data from nerve myelin. Although most phases agree with those I determined previously, there are a few differences. In this letter the two different phasings, theirs and my own, are used to compute the corresponding electron-density profile...

journal_title：Biophysical journal

authors： Blaurock AE

更新日期：1979-04-01 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00

abstract：:We present a quantitative analysis of conformational changes of the nucleotide-binding subunits, MalK(2), of the maltose ATP-binding cassette importer MalFGK(2) during the transport cycle. Distance changes occurring between selected residues were monitored in the full transporter by site-directed spin-labeling electro...

journal_title：Biophysical journal

authors： Grote M,Bordignon E,Polyhach Y,Jeschke G,Steinhoff HJ,Schneider E

更新日期：2008-09-15 00:00:00

abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

journal_title：Biophysical journal

authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

更新日期：2010-07-21 00:00:00

abstract：:The recent work is surveyed which leads to the suggestions that the conformation of globular proteins in solution corresponds to a dynamic ensemble of rapidly interconverting spatial structures, that clusters of hydrophobic amino acid side chains have an important role in the architecture of protein molecules, and tha...

journal_title：Biophysical journal

authors： Wüthrich K,Wagner G,Richarz R,Braun W

更新日期：1980-10-01 00:00:00

abstract：:We investigated whether the cysteine residues in Shaker potassium (K+) channels are essential for activation and inactivation gating or for modulation of activation gating by external zinc (Zn2+). Mutants of the Shaker K+ channel were prepared in which all seven cysteine residues were replaced (C-less). These changes ...

journal_title：Biophysical journal

authors： Boland LM,Jurman ME,Yellen G

更新日期：1994-03-01 00:00:00

abstract：:The general formalism required to treat two-state sliding filament models of muscle contraction, including free energy considerations, is first reviewed and amplified. This formalism is then used to examine, and modify as needed, three models studied previously by Podolsky and Nolan, in which cross-bridge attachment-d...

journal_title：Biophysical journal

authors： Hill TL,Eisenberg E,Chen YD,Podolsky RJ

更新日期：1975-04-01 00:00:00

abstract：:The effect of weak, nonspecific interaction between molecules confined within restricted elements of volume ("pores") and the boundary surfaces of the pore, upon the reactivity and physical state of the confined molecules, is explored by means of simple models. A confined molecule is represented by a rectangular paral...

journal_title：Biophysical journal

authors： Minton AP

更新日期：1995-04-01 00:00:00

abstract：:When cells migrate through constricting pores, they incur DNA damage and develop genomic variation. Experiments show that this damage is not due to DNA breakage from mechanical stress on chromatin in the deformed nucleus. Here we propose a model for a mechanism by which nuclear deformation can lead to DNA damage. We t...

journal_title：Biophysical journal

authors： Bennett RR,Pfeifer CR,Irianto J,Xia Y,Discher DE,Liu AJ

更新日期：2017-06-06 00:00:00

abstract：:Infrared spectroscopy has long been used to examine the average secondary structure and orientation of membrane proteins. With the recent utilization of site-specific isotope labeling (e.g., peptidic 1-(13)C = (18)O) it is now possible to examine localized properties, rather than global averages. The technique of site...

journal_title：Biophysical journal

authors： Arbely E,Kass I,Arkin IT

更新日期：2003-10-01 00:00:00

abstract：:We have studied the low-resolution solution conformation of a Holliday (or four-way) DNA junction by using small-angle x-ray scattering, sedimentation velocity, and computational modeling techniques. The scattering data were analyzed in two independent ways: firstly, by rigid-body modeling of the scattering data using...

journal_title：Biophysical journal

authors： Nöllmann M,Stark WM,Byron O

更新日期：2004-05-01 00:00:00

abstract：:During protein synthesis dictated by the codon sequence of messenger RNA, the ribosome selects aminoacyl-tRNA (aa-tRNA) with high accuracy, the exact mechanism of which remains elusive. By using a single-molecule fluorescence resonance energy transfer method coupled with fluorescence emission anisotropy, we provide ev...

journal_title：Biophysical journal

authors： Mishra PP,Qureshi MT,Ren W,Lee TH

更新日期：2010-12-01 00:00:00

abstract：:We have analyzed flash-induced period-four damped oscillation of oxygen evolution and chlorophyll fluorescence with the aid of a kinetic model of photosystem II. We have shown that, for simulation of the period-four oscillatory behavior of oxygen evolution, it is essential to consider the so-called intermediate S-stat...

journal_title：Biophysical journal

authors： Jablonsky J,Lazar D

更新日期：2008-04-01 00:00:00

abstract：:Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was develope...

journal_title：Biophysical journal

authors： Sengupta K,Aranda-Espinoza H,Smith L,Janmey P,Hammer D

更新日期：2006-12-15 00:00:00

abstract：:Nipah is an emerging paramyxovirus that is of serious concern to human health. It invades host cells using two of its membrane proteins-G and F. G binds to host ephrins and this stimulates G to activate F. Upon activation, F mediates virus-host membrane fusion. Here we focus on mechanisms that underlie the stimulation...

journal_title：Biophysical journal

authors： Dutta P,Siddiqui A,Botlani M,Varma S

更新日期：2016-10-18 00:00:00