Orientation of adsorbed cytochrome c as a function of the electrical potential of the interface studied by total internal reflection fluorescence.

abstract：:In the budding yeast nucleus, transfer RNA (tRNA) genes are considered to localize in the vicinity of the nucleolus; however, the use of Hi-C and fluorescent repressor-operator system techniques has clearly indicated that the tRNA genes are distributed not only around the nucleolus but also at other nuclear locations....

journal_title：Biophysical journal

authors： Tokuda N

更新日期：2020-05-05 00:00:00

abstract：:Mathematical models are increasingly important in biology, and testability is becoming a critical issue. One limitation is that one model simulation tests a parameter set representing one instance of the biological counterpart, whereas biological systems are heterogeneous in their properties and behavior, and a model ...

journal_title：Biophysical journal

authors： Tomaiuolo M,Bertram R,Leng G,Tabak J

更新日期：2012-11-07 00:00:00

abstract：:The mouse major urinary protein (MUP) has proved to be an intriguing test bed for detailed studies on protein-ligand recognition. NMR, calorimetric, and modeling investigations have revealed that the thermodynamics of ligand binding involve a complex interplay between competing enthalpic and entropic terms. We perform...

journal_title：Biophysical journal

authors： Roy J,Laughton CA

更新日期：2010-07-07 00:00:00

abstract：:It has been repeatedly noted that the change of conformation of the molecules that serve as the ion-selective channels for sodium and potassium conductance in the nerve membrane will be accompanied by a change in the dipole moment of the molecule. This time-dependent change of dipole moment will produce transient curr...

journal_title：Biophysical journal

authors： Strandberg MW

更新日期：1977-09-01 00:00:00

abstract：:Biological membranes are constantly exposed to forces. The stress-strain relation in membranes determines the behavior of many integral membrane proteins or other membrane related-proteins that show a mechanosensitive behavior. Here, we studied by force spectroscopy the behavior of supported lipid bilayers (SLBs) subj...

journal_title：Biophysical journal

authors： Alessandrini A,Seeger HM,Caramaschi T,Facci P

更新日期：2012-07-03 00:00:00

abstract：:To explain the effect of profilin on actin critical concentration in a manner consistent with thermodynamic constraints and available experimental data, we built a thermodynamically rigorous model of actin steady-state dynamics in the presence of profilin. We analyzed previously published mechanisms theoretically and ...

journal_title：Biophysical journal

authors： Yarmola EG,Dranishnikov DA,Bubb MR

更新日期：2008-12-15 00:00:00

abstract：:We present higher-order moment analysis of fluorescence intensity fluctuations from individual laser scanning microscopy images applied to study monomer-oligomer distributions. We demonstrate that the number densities and brightness ratios of a mixed population of monomers and oligomers can be determined by analyzing ...

journal_title：Biophysical journal

authors： Sergeev M,Costantino S,Wiseman PW

更新日期：2006-11-15 00:00:00

abstract：:Structural transition can be induced in charged micelles by increasing the ionic strength of the medium. We have monitored the organization and dynamics of the functionally important tryptophan residues of gramicidin in spherical and rod-shaped sodium dodecyl sulfate micelles utilizing a combination of wavelength-sele...

journal_title：Biophysical journal

authors： Rawat SS,Kelkar DA,Chattopadhyay A

更新日期：2005-11-01 00:00:00

abstract：:A 3D model was developed and used to explore dynamics of phosphatidylinositol-4,5-bisphosphate (PIP2) signaling in cerebellar Purkinje neurons. Long-term depression in Purkinje neurons depends on coincidence detection of climbing fiber stimulus evoking extracellular calcium flux into the cell and parallel fiber stimul...

journal_title：Biophysical journal

authors： Brown SA,Morgan F,Watras J,Loew LM

更新日期：2008-08-01 00:00:00

abstract：:To monitor the lateral segregation of lipids into liquid-ordered (Lo) and -disordered (Ld) phases in lipid membranes, environment-sensitive dyes that partition in both phases but stain them differently have been developed. Of particular interest is the dual-color F2N12S probe, which can discriminate the two phases thr...

journal_title：Biophysical journal

authors： Kilin V,Glushonkov O,Herdly L,Klymchenko A,Richert L,Mely Y

更新日期：2015-05-19 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the ...

journal_title：Biophysical journal

authors： Zasadzinski JA,Alig TF,Alonso C,Bernardino de la Serna J,Perez-Gil J,Taeusch HW

更新日期：2005-09-01 00:00:00

abstract：:Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuse...

journal_title：Biophysical journal

authors： Mashl RJ,Bruinsma RF

更新日期：1998-06-01 00:00:00

abstract：:A subsarcolemmal tubular system network (SSTN) has been detected in skeletal muscle fibers by confocal imaging after the removal of the sarcolemma. Here we confirm the existence and resolve the fine architecture and the localization of the SSTN at an unprecedented level of detail by examining extracellularly applied t...

journal_title：Biophysical journal

authors： Jayasinghe ID,Lo HP,Morgan GP,Baddeley D,Parton RG,Soeller C,Launikonis BS

更新日期：2013-06-04 00:00:00

abstract：:General anesthesia is a relatively safe medical procedure, which for nearly 170 years has allowed life saving surgical interventions in animals and people. However, the molecular mechanism of general anesthesia continues to be a matter of importance and debate. A favored hypothesis proposes that general anesthesia res...

journal_title：Biophysical journal

authors： Covarrubias M,Barber AF,Carnevale V,Treptow W,Eckenhoff RG

更新日期：2015-11-17 00:00:00

abstract：:Structure factor amplitudes and phases can be computed directly from electron cryomicroscopy images. Inherent aberrations of the electromagnetic lenses and other instrumental factors affect the structure factors, however, resulting in decreased accuracy in the determined three-dimensional reconstruction. In contrast, ...

journal_title：Biophysical journal

authors： Thuman-Commike PA,Tsuruta H,Greene B,Prevelige PE Jr,King J,Chiu W

更新日期：1999-04-01 00:00:00

abstract：:In this article, we explore the information content of molecular force-field calculations. We make use of exhaustive lattice models of molecular conformations and reduced alphabet sequences to determine the relative resolving power of pairwise interaction-based force fields. We find that sequence-specific interactions...

journal_title：Biophysical journal

authors： Aynechi T,Kuntz ID

更新日期：2005-11-01 00:00:00

abstract：:A continuum model is provided of the free energy terms that contribute to the molecular association of ricin A-chain (RTA) with the rRNA substrate and several small ligands. The model for RTA interactions with the RNA was taken from a previously proposed complex containing a 29-mer oligonucleotide hairpin (. Proteins ...

journal_title：Biophysical journal

authors： Olson MA,Cuff L

更新日期：1999-01-01 00:00:00

abstract：:Influenza A virus M2 protein is known to form acid-activated, proton-selective, amantadine-sensitive channels. We directly measured proton uptake in vesicles containing reconstituted M2 by monitoring external pH after addition of valinomycin to vesicles with 100-fold-diluted external [K(+)]. External pH typically incr...

journal_title：Biophysical journal

authors： Moffat JC,Vijayvergiya V,Gao PF,Cross TA,Woodbury DJ,Busath DD

更新日期：2008-01-15 00:00:00

abstract：:Combining an azobenzene chromophore with the bis-cysteinyl active-site sequence of the protein disulfide isomerase (PDI) we constructed a simple but promising model for allosteric conformational rearrangements. Paralleling cellular signaling events, an external trigger, here absorption of a photon, leads to a structur...

journal_title：Biophysical journal

authors： Löweneck M,Milbradt AG,Root C,Satzger H,Zinth W,Moroder L,Renner C

更新日期：2006-03-15 00:00:00

abstract：:Based on differences between the x-ray crystal structures of ligand-bound and unbound forms, the activation of the erythropoietin receptor (EPOR) was initially proposed to involve a cross-action scissorlike motion. However, the validity of the motions involved in the scissorlike model has been recently challenged. Her...

journal_title：Biophysical journal

authors： Corbett MSP,Mark AE,Poger D

更新日期：2017-02-28 00:00:00

abstract：:A detailed picture of water and ion properties in small pores is important for understanding the behavior of biological ion channels. Several recent modeling studies have shown that small, hydrophobic pores exclude water and ions even if they are physically large enough to accommodate them, a mechanism called hydropho...

journal_title：Biophysical journal

authors： Spronk SA,Elmore DE,Dougherty DA

更新日期：2006-05-15 00:00:00

abstract：:The aggregation of amyloid-β (Aβ) peptides plays a crucial role in the etiology of Alzheimer's disease (AD). Recently, it has been reported that an A2T mutation in Aβ can protect against AD. Interestingly, a nonpolar A2V mutation also has been found to offer protection against AD in the heterozygous state, although it...

journal_title：Biophysical journal

authors： Das P,Murray B,Belfort G

更新日期：2015-02-03 00:00:00

abstract：:The dynamics of protein distribution in endocytic membranes are relevant for many cellular processes, such as protein sorting, organelle and membrane microdomain biogenesis, protein-protein interactions, receptor function, and signal transduction. We have developed an assay based on Fluorescence Resonance Energy Micro...

journal_title：Biophysical journal

authors： Wallrabe H,Elangovan M,Burchard A,Periasamy A,Barroso M

更新日期：2003-07-01 00:00:00

abstract：:High resolution dynamics and structural information has been resolved from 2H solid-state NMR spectra of the Val-1 side-chain of the gramicidin channel in a lipid bilayer. Both powder pattern lineshapes and spectra from uniformly aligned samples of gramicidin in lipid bilayers have been analyzed to achieve a fully con...

journal_title：Biophysical journal

authors： Lee KC,Cross TA

更新日期：1994-05-01 00:00:00

abstract：:Cord factor (trehalose 6,6'-dimycolate, CF) is a glycolipid located in the outer mycobacterial cell wall that is implicated in the pathogenesis of mycobacteria. Furthermore, CF is a convenient model for studying mycolic acid residues, the major lipid constituents of the mycobacterial cell wall that are believed to for...

journal_title：Biophysical journal

authors： Almog R,Mannella CA

更新日期：1996-12-01 00:00:00

abstract：:The optical scattering coefficient of a dilute, well-solubilized eumelanin solution has been accurately measured as a function of incident wavelength, and found to contribute <6% of the total optical attenuation between 210 and 325 nm. At longer wavelengths (325-800 nm), the scattering was less than the minimum sensit...

journal_title：Biophysical journal

authors： Riesz J,Gilmore J,Meredith P

更新日期：2006-06-01 00:00:00

abstract：:The 77-K resonance Raman vibrational spectrum of intact goldfish rod photoreceptors containing 3,4-dehydro (A2) retinal is dominated by scattering from the 9-cis component of the steady state at all excitation wavelengths. Intact goldfish photoreceptors were regenerated with an A1-retinal chromophore to determine whet...

journal_title：Biophysical journal

authors： Barry B,Mathies RA,Pardoen JA,Lugtenburg J

更新日期：1987-10-01 00:00:00

abstract：:Surface charge in track-etched polyethylene terephthalate (PET) membranes with narrow pores has been probed with a fluorescent cationic dye (3,3'-diethyloxacarbocyanine iodide (diO-C2-(3))) using confocal microscopy. Staining of negatively charged PET membranes with diO-C2-(3) is a useful measure of surface charge for...

journal_title：Biophysical journal

authors： Bashford CL,Alder GM,Pasternak CA

更新日期：2002-04-01 00:00:00

abstract：:We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displa...

journal_title：Biophysical journal

authors： Terpetschnig E,Szmacinski H,Malak H,Lakowicz JR

更新日期：1995-01-01 00:00:00