Abstract:
:A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Zasadzinski JA,Alig TF,Alonso C,Bernardino de la Serna J,Perez-Gil J,Taeusch HWdoi
10.1529/biophysj.105.062646subject
Has Abstractpub_date
2005-09-01 00:00:00pages
1621-9issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(05)72808-3journal_volume
89pub_type
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