Abstract:
:The mating pathway in Saccharomyces cerevisiae is one of the best understood signal transduction pathways in eukaryotes. It transmits the mating signal from plasma membrane into the nucleus through the G-protein coupled receptor and the mitogen-activated protein kinase (MAPK) cascade. According to current understanding of the mating pathway, we construct a system of ordinary differential equations to describe the process. Our model is consistent with a wide range of experiments, indicating that it captures some main characteristics of the signal transduction along the pathway. Investigation with the model reveals that the shuttling of the scaffold protein and the dephosphorylation of kinases involved in the MAPK cascade cooperate to regulate the response upon pheromone induction and to help preserve the fidelity of the mating signaling. We explored factors affecting the dose-response curves of this pathway and found that both negative feedback and concentrations of the proteins involved in the MAPK cascade play crucial roles. Contrary to some other MAPK systems where signaling sensitivity is being amplified successively along the cascade, here the mating signal is transmitted through the cascade in an almost linear fashion.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Shao D,Zheng W,Qiu W,Ouyang Q,Tang Cdoi
10.1529/biophysj.106.081661subject
Has Abstractpub_date
2006-12-01 00:00:00pages
3986-4001issue
11eissn
0006-3495issn
1542-0086pii
S0006-3495(06)72115-4journal_volume
91pub_type
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