Carcinoma and SV40-transfected normal ovarian surface epithelial cell comparison by nonphotochemical hole burning.

abstract：:Gastrointestinal slow waves are generated within networks of interstitial cells of Cajal (ICCs). In the intact tissue, slow waves are entrained to neighboring ICCs with higher intrinsic frequencies, leading to active propagation of slow waves. Degradation of ICC networks in humans is associated with motility disorders...

journal_title：Biophysical journal

authors： Du P,O'Grady G,Gibbons SJ,Yassi R,Lees-Green R,Farrugia G,Cheng LK,Pullan AJ

更新日期：2010-05-19 00:00:00

abstract：:Emerging evidence indicates that mitochondrial flashes (mitoflashes) are highly conserved elemental mitochondrial signaling events. However, which signal controls their ignition and how they are integrated with other mitochondrial signals and functions remain elusive. In this study, we aimed to further delineate the s...

journal_title：Biophysical journal

authors： Wang X,Zhang X,Huang Z,Wu D,Liu B,Zhang R,Yin R,Hou T,Jian C,Xu J,Zhao Y,Wang Y,Gao F,Cheng H

更新日期：2016-07-26 00:00:00

abstract：:Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the su...

journal_title：Biophysical journal

authors： Muhlrad A,Ringel I,Pavlov D,Peyser YM,Reisler E

更新日期：2006-12-15 00:00:00

abstract：:Intrinsic optical signal (IOS) imaging has been widely used to map the patterns of brain activity in vivo in a label-free manner. Traditional IOS refers to changes in light transmission, absorption, reflectance, and scattering of the brain tissue. Here, we use polarized light for IOS imaging to monitor structural chan...

journal_title：Biophysical journal

authors： Koike-Tani M,Tominaga T,Oldenbourg R,Tani T

更新日期：2020-05-19 00:00:00

abstract：:An innovative method is presented to measure the mechanical driving point impedance of biological structures up to at least 40 kHz. The technique employs an atomic force cantilever with a ferromagnetic coating and an external magnetic field to apply a calibrated force to the cantilever. Measurement of the resulting ca...

journal_title：Biophysical journal

authors： Scherer MP,Gummer AW

更新日期：2004-08-01 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00

abstract：:Voltage-sensing dyes and voltage-sensing fluorescence proteins have been continually improved and as a result have provided a wealth of insights into neuronal circuits. Further improvements in voltage-sensing dyes and voltage-sensing fluorescence proteins are needed, however, for routine detection of single action pot...

journal_title：Biophysical journal

authors： Park K,Weiss S

更新日期：2017-02-28 00:00:00

abstract：:Microbes inhabiting Earth have adapted to diverse environments of water, air, soil, and often at the interfaces of multiple media. In this study, we focus on the behavior of Caulobacter crescentus, a singly flagellated bacterium, at the air/water interface. Forward swimming C. crescentus swarmer cells tend to get phys...

journal_title：Biophysical journal

authors： Morse M,Huang A,Li G,Maxey MR,Tang JX

更新日期：2013-07-02 00:00:00

abstract：:The kinetics of methyl-, ethyl-, iso-propyl-, and ter-butyl-isocyanide binding to Aplysia limacina myoglobin (distal His----Lys) and the isolated beta chains from hemoglobin Zurich (distal His----Arg) have been investigated by flash photolysis at various temperatures above 0 degrees C. Sperm whale (Physter catodon) my...

journal_title：Biophysical journal

authors： Di Iorio EE,Winterhalter KH,Giacometti GM

更新日期：1987-03-01 00:00:00

abstract：:The present article considers the influence of heterogeneity in a mobile analyte or in an immobilized ligand population on the surface binding kinetics and equilibrium isotherms. We describe strategies for solving the inverse problem of calculating two-dimensional distributions of rate and affinity constants from expe...

journal_title：Biophysical journal

authors： Svitel J,Balbo A,Mariuzza RA,Gonzales NR,Schuck P

更新日期：2003-06-01 00:00:00

abstract：:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily...

journal_title：Biophysical journal

authors： Casuso I,Sens P,Rico F,Scheuring S

更新日期：2010-10-06 00:00:00

abstract：:There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In additio...

journal_title：Biophysical journal

authors： Jiang Y,Rabbi M,Kim M,Ke C,Lee W,Clark RL,Mieczkowski PA,Marszalek PE

更新日期：2009-02-01 00:00:00

abstract：:Cryo-electron microscopy is rapidly emerging as a powerful technique to determine the structures of complex macromolecular systems elusive to other techniques. Because many of these systems are highly dynamical, characterizing their movements is also a crucial step to unravel their biological functions. To achieve thi...

journal_title：Biophysical journal

authors： Bonomi M,Pellarin R,Vendruscolo M

更新日期：2018-04-10 00:00:00

abstract：:Ras is a membrane-anchored signaling protein that serves as a hub for many signaling pathways and also plays a prominent role in cancer. The intrinsic behavior of Ras on the membrane has captivated the biophysics community in recent years, especially the possibility that it may form dimers. In this article, we describ...

journal_title：Biophysical journal

authors： Chung JK,Lee YK,Denson JP,Gillette WK,Alvarez S,Stephen AG,Groves JT

更新日期：2018-01-09 00:00:00

abstract：:Stochastic effects in gene expression may result in different physiological states of individual cells, with consequences for pathogen survival and artificial gene network design. We studied the contributions of a regulatory factor to gene expression noise in four basic mechanisms of negative gene expression control: ...

journal_title：Biophysical journal

authors： Komorowski M,Miekisz J,Kierzek AM

更新日期：2009-01-01 00:00:00

abstract：:Cell adhesion often occurs under dynamic conditions, as in flowing blood. A quantitative understanding of this process requires accurate knowledge of the topographical relationships between the cell membrane and potentially adhesive surfaces. This report describes an experimental study made on both the translational a...

journal_title：Biophysical journal

authors： Tissot O,Pierres A,Foa C,Delaage M,Bongrand P

更新日期：1992-01-01 00:00:00

abstract：:The brain Na+ channel-1 (BNC1, also known as MDEG1 or ASIC2) is a member of the DEG/ENaC cation channel family. Mutation of a specific residue (Gly430) that lies N-terminal to the second membrane-spanning domain activates BNC1 and converts it from a Na+-selective channel to one permeable to both Na+ and K+. Because al...

journal_title：Biophysical journal

authors： Adams CM,Price MP,Snyder PM,Welsh MJ

更新日期：1999-03-01 00:00:00

abstract：:A number of properties of certain living embryonic tissues can be explained by considering them as liquids. Tissue fragments left in a shaker bath round up to form spherical aggregates, as do liquid drops. When cells comprising two distinct embryonic tissues are mixed, typically a nucleation-like process takes place, ...

journal_title：Biophysical journal

authors： Forgacs G,Foty RA,Shafrir Y,Steinberg MS

更新日期：1998-05-01 00:00:00

abstract：:ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

journal_title：Biophysical journal

authors： Cherepanov DA,Junge W

更新日期：2001-09-01 00:00:00

abstract：:Interactions between amyloid-β peptide (Aβ) and the cell membrane include interaction with membrane lipids and binding to membrane receptors, both of which are considered to be the toxicity mechanisms of Aβ. However, it is unclear whether both mechanisms lead to cytotoxicity. Thus, we aimed to analyze these two mechan...

journal_title：Biophysical journal

authors： Shi JM,Zhu L,Lan X,Zhao DW,He YJ,Sun ZQ,Wu D,Li HY

更新日期：2020-09-15 00:00:00

abstract：:Five molecular dynamics computer simulations were performed on different phospholipid:sterol membrane systems in order to study the influence of sterol structure on membrane properties. Three of these simulated bilayer systems were composed of a 1:8 sterol:phospholipid ratio, each of which employed one of the sterol m...

journal_title：Biophysical journal

authors： Smondyrev AM,Berkowitz ML

更新日期：2001-04-01 00:00:00

abstract：:We present MEMLET (MATLAB-enabled maximum-likelihood estimation tool), a simple-to-use and powerful program for utilizing maximum-likelihood estimation (MLE) for parameter estimation from data produced by single-molecule and other biophysical experiments. The program is written in MATLAB and includes a graphical user ...

journal_title：Biophysical journal

authors： Woody MS,Lewis JH,Greenberg MJ,Goldman YE,Ostap EM

更新日期：2016-07-26 00:00:00

abstract：:Determining the spatial relationship of individual proteins in dense assemblies remains a challenge for superresolution nanoscopy. The organization of aquaporin-4 (AQP4) into large plasma membrane assemblies provides an opportunity to image membrane-bound AQP4 antibodies (AQP4-IgG) and evaluate changes in their spatia...

journal_title：Biophysical journal

authors： Soltys JN,Meyer SA,Schumann H,Gibson EA,Restrepo D,Bennett JL

更新日期：2017-04-25 00:00:00

abstract：:The equation of motion for sperm flagella, in which the elastic bending moment and the active contractile moment are balanced by the moment from the viscous resistance of the surrounding fluid, is solved for a wave solution that superimposes partial solutions. Substitution of the expression for the wave solution into ...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1978-08-01 00:00:00

abstract：:In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially ...

journal_title：Biophysical journal

authors： Greiss F,Deligiannaki M,Jung C,Gaul U,Braun D

更新日期：2016-02-23 00:00:00

abstract：:The aggregation of alpha-synuclein (α-syn) and huntingtin (htt) into fibrillar assemblies in nerve and glial cells is a molecular hallmark of Parkinson's and Huntington's diseases. Within the aggregation process, prefibrillar and fibrillar oligomeric species form. Prefibrillar assemblies rather than fibrils are nowada...

journal_title：Biophysical journal

authors： Pieri L,Madiona K,Bousset L,Melki R

更新日期：2012-06-20 00:00:00

abstract：:We propose mechanisms by which the transmembrane domain of vesicular stomatitis virus (VSV-TMD) promotes both initiation of fusion and formation of a fusion pore. Time courses of polyethyleneglycol (PEG)-mediated fusion of 25 nm small unilamellar vesicles composed of dioleoylphosphatidylcholine, dioleoylphosphatidylet...

journal_title：Biophysical journal

authors： Sengupta T,Chakraborty H,Lentz BR

更新日期：2014-09-16 00:00:00

abstract：:The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel swimming bacteria. Extensive experimental and theoretical studies exist on the structure, assembly, energy input, power generation, and switching mechanism of the motor. In a previous article, we explained the ge...

journal_title：Biophysical journal

authors： Bai F,Lo CJ,Berry RM,Xing J

更新日期：2009-04-22 00:00:00

abstract：:We present a molecular model of a chromatophore vesicle from Rhodobacter sphaeroides. These vesicles are ideal benchmark systems for molecular and systemic simulations, because they have been well studied, they are small, and they are naturally separated from their cellular environment. To set up a photosynthetic chai...

journal_title：Biophysical journal

authors： Geyer T,Helms V

更新日期：2006-08-01 00:00:00

abstract：:Thermodynamic analysis of metabolic networks has recently generated increasing interest for its ability to add constraints on metabolic network operation, and to combine metabolic fluxes and metabolite measurements in a mechanistic manner. Concepts for the calculation of the change in Gibbs energy of biochemical react...

journal_title：Biophysical journal

authors： Jol SJ,Kümmel A,Hatzimanikatis V,Beard DA,Heinemann M

更新日期：2010-11-17 00:00:00