Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors.

Abstract:

:Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Braun T,Backmann N,Vögtli M,Bietsch A,Engel A,Lang HP,Gerber C,Hegner M

doi

10.1529/biophysj.105.072934

subject

Has Abstract

pub_date

2006-04-15 00:00:00

pages

2970-7

issue

8

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(06)72477-8

journal_volume

90

pub_type

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