Abstract:
:Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Braun T,Backmann N,Vögtli M,Bietsch A,Engel A,Lang HP,Gerber C,Hegner Mdoi
10.1529/biophysj.105.072934subject
Has Abstractpub_date
2006-04-15 00:00:00pages
2970-7issue
8eissn
0006-3495issn
1542-0086pii
S0006-3495(06)72477-8journal_volume
90pub_type
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