Binding modes of μ-conotoxin to the bacterial sodium channel (NaVAb).

abstract：:Hemoglobin functions as a tetrameric oxygen transport protein, with each subunit containing a heme cofactor. Its denaturation, either in vivo or in vitro, involves autoxidation to methemoglobin, followed by cofactor loss and globin unfolding. We have proposed a global disassembly scheme for human methemoglobin, linkin...

journal_title：Biophysical journal

authors： Samuel PP,White MA,Ou WC,Case DA,Phillips GN Jr,Olson JS

更新日期：2020-03-24 00:00:00

abstract：:We describe a model that relates the maximum shortening velocity of a muscle fiber, Vm, to the kinetics of the dissociation of a myosin head from actin. At Vm, the positive work exerted by cross-bridges attached in the powerstroke must be balanced by cross-bridges that have been carried by movement of the filaments in...

journal_title：Biophysical journal

authors： Cooke R,White H,Pate E

更新日期：1994-03-01 00:00:00

abstract：:The antagonizing effect of high pressure against anesthesia is well known. With purified firefly luciferase, however,. Biophys. J. 60:1309-1314) reported that high pressure did not affect the initial flash intensity. Firefly luciferase emits a burst of light when the substrates luciferin and ATP are added in the prese...

journal_title：Biophysical journal

authors： Ueda I,Minami H,Matsuki H,Inoue T

更新日期：1999-01-01 00:00:00

abstract：:Changes in free energy are normally used to track the effect of temperature on the stability of proteins and hydrophobic interactions. Use of this procedure on the aqueous solubility of hydrocarbons, a standard representation of the hydrophobic effect, leads to the conclusion that the hydrophobic effect increases in s...

journal_title：Biophysical journal

authors： Schellman JA

更新日期：1997-12-01 00:00:00

abstract：:Molecular dynamics simulations have been performed of the sequence-symmetric cyclic decapeptide antibiotic gramicidin S (GS), in interaction with a hydrated dimyristoylphosphatidylcholine (DMPC) bilayer, and the results compared with a "control" simulation of the system in the absence of GS. Following experimental evi...

journal_title：Biophysical journal

authors： Mihailescu D,Smith JC

更新日期：2000-10-01 00:00:00

abstract：:Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particles similar to nascent high-density lipoproteins, which reduce the risk of coronary artery disease. The major protein component of high-density lipoproteins is human apolipoprotein A-I, and the corresponding protein component of Nanodis...

journal_title：Biophysical journal

authors： Li Y,Kijac AZ,Sligar SG,Rienstra CM

更新日期：2006-11-15 00:00:00

abstract：:Antifreeze proteins (AFPs) protect certain organisms from freezing by adhering to ice crystals, thereby preventing their growth. All AFPs depress the nonequilibrium freezing temperature below the melting point; however AFPs from overwintering insects, such as the spruce budworm (sbw) are 10-100 times more effective th...

journal_title：Biophysical journal

authors： Pertaya N,Marshall CB,Celik Y,Davies PL,Braslavsky I

更新日期：2008-07-01 00:00:00

abstract：:Because of their remarkable ability to depress the freezing point of aqueous solutions, antifreeze proteins (AFPs) play a critical role in helping many organisms survive subzero temperatures. The beta-helical insect AFP structures solved to date, consisting of multiple repeating circular loops or coils, are perhaps th...

journal_title：Biophysical journal

authors： Liu K,Jia Z,Chen G,Tung C,Liu R

更新日期：2005-02-01 00:00:00

abstract：:Fluorescence lifetime imaging microscopy is used to demonstrate that different loads applied to a muscle fiber change the microenvironment of the nucleotide binding pocket of myosin. Permeabilized skeletal muscle fibers in rigor were labeled with a fluorescent ATP analog, 3'-DEAC-propylenediamine (pda)-ATP (3'-O-{N-[3...

journal_title：Biophysical journal

authors： Ibanez-Garcia D,Requejo-Isidro J,Webb MR,West TG,French P,Ferenczi MA

更新日期：2010-10-06 00:00:00

abstract：:Molecular chaperone Hsp70 plays important roles in the pathology of amyloid diseases by inhibiting aberrant aggregation of proteins. However, the biophysical mechanism of the interaction of Hsp70 with the intrinsically disordered proteins (IDPs) is unclear. Here, we report that Hsp70 inhibits aggregation of islet amyl...

journal_title：Biophysical journal

authors： Chilukoti N,Sil TB,Sahoo B,Deepa S,Cherakara S,Maddheshiya M,Garai K

更新日期：2021-01-06 00:00:00

abstract：:A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found for the various parts of the life cycle: S, 4.13 hours; G1, 4.71 hours; G2, 2.81 hours; mitosis, 0.81 hours. The cell has a to...

journal_title：Biophysical journal

authors： PUCK TT,SANDERS P,PETERSEN D

更新日期：1964-11-01 00:00:00

abstract：:The light-harvesting complex I (LH-I) of Rhodobacter sphaeroides has been modeled computationally as a hexadecamer of alphabeta-heterodimers, based on a close homology of the heterodimer to that of light-harvesting complex II (LH-II) of Rhodospirillum molischianum. The resulting LH-I structure yields an electron densi...

journal_title：Biophysical journal

authors： Hu X,Schulten K

更新日期：1998-08-01 00:00:00

abstract：:To explain the effect of profilin on actin critical concentration in a manner consistent with thermodynamic constraints and available experimental data, we built a thermodynamically rigorous model of actin steady-state dynamics in the presence of profilin. We analyzed previously published mechanisms theoretically and ...

journal_title：Biophysical journal

authors： Yarmola EG,Dranishnikov DA,Bubb MR

更新日期：2008-12-15 00:00:00

abstract：:The one-dimensional information of genomic DNA is hierarchically packed inside the eukaryotic cell nucleus and organized in a three-dimensional (3D) space. Genome-wide chromosome conformation capture (Hi-C) methods have uncovered the 3D genome organization and revealed multiscale chromatin domains of compartments and ...

journal_title：Biophysical journal

authors： Shinkai S,Sugawara T,Miura H,Hiratani I,Onami S

更新日期：2020-05-05 00:00:00

abstract：:A new method, weighted-ensemble Brownian dynamics, is proposed for the simulation of protein-association reactions and other events whose frequencies of outcomes are constricted by free energy barriers. The method features a weighted ensemble of trajectories in configuration space with energy levels dictating the prop...

journal_title：Biophysical journal

authors： Huber GA,Kim S

更新日期：1996-01-01 00:00:00

abstract：:We present a single virion method to determine absolute distributions of copy number in the protein composition of viruses and apply it to herpes simplex virus type 1. Using two-color coincidence fluorescence spectroscopy, we determine the virion-to-virion variability in copy numbers of fluorescently labeled tegument ...

journal_title：Biophysical journal

authors： Clarke RW,Monnier N,Li H,Zhou D,Browne H,Klenerman D

更新日期：2007-08-15 00:00:00

abstract：:Inactivation is a fundamental property of voltage-gated ion channels. Fast inactivation of Na(+) channels involves channel block by the III-IV cytoplasmic interdomain linker. The mechanisms of nonfast types of inactivation (intermediate, slow, and ultraslow) are unclear, although the ionic environment and P-loops rear...

journal_title：Biophysical journal

authors： Tikhonov DB,Zhorov BS

更新日期：2007-09-01 00:00:00

abstract：:Mechanical and two-dimensional (2D) x-ray diffraction studies suggest that during isometric steady-state contraction, strongly bound cross-bridges mostly occupy early states in the power stroke, whereas rigor or rigor-like cross-bridges could not be detected. However, it remained unclear whether cross-bridges accumula...

journal_title：Biophysical journal

authors： Radocaj A,Weiss T,Helsby WI,Brenner B,Kraft T

更新日期：2009-02-18 00:00:00

abstract：:Glutamic acid [(L-glu)n] + dihydrogen phosphate systems are studied by infrared (IR) spectroscopy dried and hydrated at 75% relative humidity, as a function of both the phosphate-glutamic acid residue (Pi/glu) ratio and the type of cations present. It is shown that the glutamic acid groups form hydrogen-bonded chains ...

journal_title：Biophysical journal

authors： Burget U,Zundel G

更新日期：1987-12-01 00:00:00

abstract：:Small-angle neutron scattering (SANS) is used to probe the solution structure of two protein therapeutics (monoclonal antibodies 1 and 2 (MAb1 and MAb2)) and their protein-protein interaction (PPI) at high concentrations. These MAbs differ by small sequence alterations in the complementarity-determining region but sho...

journal_title：Biophysical journal

authors： Yearley EJ,Zarraga IE,Shire SJ,Scherer TM,Gokarn Y,Wagner NJ,Liu Y

更新日期：2013-08-06 00:00:00

abstract：:Forces between type I collagen triple helices are studied in solvents of varying hydrogen-bonding ability. The swelling of collagen fibers in reconstituted films is controlled by the concentration of soluble polymers that are excluded from the fibers and that compete osmotically with collagen for available solvent. Th...

journal_title：Biophysical journal

authors： Kuznetsova N,Rau DC,Parsegian VA,Leikin S

更新日期：1997-01-01 00:00:00

abstract：:Ataxin-1 is a human protein responsible for spinocerebellar ataxia type 1, a hereditary disease associated with protein aggregation and misfolding. Essential for ataxin-1 aggregation is the anomalous expansion of a polyglutamine tract near the protein N-terminus, but the sequence-wise distant AXH domain modulates and ...

journal_title：Biophysical journal

authors： de Chiara C,Rees M,Menon RP,Pauwels K,Lawrence C,Konarev PV,Svergun DI,Martin SR,Chen YW,Pastore A

更新日期：2013-03-19 00:00:00

abstract：:Fiber orientation and ventricular geometry are incorporated in a mathematical model for ejection fraction of the human left ventricle. The inadequacy of circularly oriented or "constrictor" fibers to explain high ejection fractions of cylindrical, spherical, or ellipsoidal reference ventricles is demonstrated. A class...

journal_title：Biophysical journal

authors： Sallin EA

更新日期：1969-07-01 00:00:00

abstract：:Cardiac transverse (T)-tubules provide a specialized structure for synchronization and stabilization of sarcoplasmic reticulum Ca(2+) release in healthy cardiomyocytes. The application of laser scanning confocal microscopy and the use of fluorescent lipophilic membrane dyes have boosted the discoveries that T-tubule r...

journal_title：Biophysical journal

authors： Guo A,Song LS

更新日期：2014-06-17 00:00:00

abstract：:We have studied the anion-dependent gating of roflamycoin ion channels using spectral analysis of noise in currents through multichannel planar lipid bilayers. We have found that in addition to low frequency current fluctuations that may be attributed to channel switching between open and closed conformations, roflamy...

journal_title：Biophysical journal

authors： Grigorjev PA,Bezrukov SM

更新日期：1994-12-01 00:00:00

abstract：:The serine protease inhibitor antithrombin undergoes extensive conformational changes during functional interaction with its target proteases. Changes include insertion of the reactive loop region into a beta-sheet structure in the protein core. We explore the possibility that these changes are linked to water transfe...

journal_title：Biophysical journal

authors： Liang J,McGee MP

更新日期：1998-08-01 00:00:00

abstract：:Phase-resolved monitoring of photoacoustic signals can provide information about the depth profile of a sample. We describe an application of this principle to determine the depth profiles of ligands and antitumor agents in mammalian cells. Measurements of the in-phase and quadrature components of the photoacoustic sp...

journal_title：Biophysical journal

authors： Narayanan K,Chandani S,Ramakrishna T,Rao CM

更新日期：1997-05-01 00:00:00

abstract：:In order to verify the "radiation protection" effect of prosthetic groups in irradiated conjugated proteins and the influence of their electronic structure on intramolecular excitation transfer, two series of substances were examined. The first was composed of ferrihemoglobin and its constituents and the second was co...

journal_title：Biophysical journal

authors： Crippa PR,Vecli A

更新日期：1970-03-01 00:00:00

abstract：:An ab initio model for gene prediction in prokaryotic genomes is proposed based on physicochemical characteristics of codons calculated from molecular dynamics (MD) simulations. The model requires a specification of three calculated quantities for each codon: the double-helical trinucleotide base pairing energy, the b...

journal_title：Biophysical journal

authors： Singhal P,Jayaram B,Dixit SB,Beveridge DL

更新日期：2008-06-01 00:00:00

abstract：:This study aims to define the role of E-cadherin (Ecad) engagement in cell-cell contact during membrane-cortex interaction. As a tool, we used a hydrodynamic membrane tube extrusion technique to characterize the mechanical interaction between the plasma membrane and the underlying cortical cytoskeleton. Cells were anc...

journal_title：Biophysical journal

authors： Tabdanov E,Borghi N,Brochard-Wyart F,Dufour S,Thiery JP

更新日期：2009-03-18 00:00:00