Rapid delivery of small interfering RNA by biosurfactant MEL-A-containing liposomes.


:The downregulation of gene expression by RNA interference holds great potential for genetic analysis and gene therapy. However, a more efficient delivery system for small interfering RNA (siRNA) into the target cells is required for wide fields such as cell biology, physiology, and clinical application. Non-viral vectors are stronger candidates than viral vectors because they are safer and easier to prepare. We have previously used a new method for gene transfection by combining cationic liposomes with the biosurfactant mannosylerythritol lipid-A (MEL-A). The novel MEL-A-containing cationic liposomes rapidly delivered DNA (plasmids and oligonucleotides) into the cytosol and nucleus through membrane fusion between liposomes and the plasma membrane, and consequently, enhanced the gene transfection efficiency. In this study, we determined the efficiency of MEL-A-containing cationic liposomes for siRNA delivery. We observed that exogenous and endogenous protein expression was suppressed by approximately 60% at 24h after brief (30 min) incubation of target cells with MEL-A-containing cationic liposome/siRNA complexes. Confocal microscopic analysis showed that suppression of protein expression was caused by rapid siRNA delivery into the cytosol. We found that the MEL-A-containing cationic liposomes directly delivered siRNA into the cytoplasm by the membrane fusion in addition to endocytotic pathway whereas Lipofectamine RNAiMax delivered siRNA only by the endocytotic pathway. It seems that the ability to rapidly and directly deliver siRNA into the cytosol using MEL-A-containing cationic liposomes is able to reduce immune responses, cytotoxicity, and other side effects caused by viral vectors in clinical applications.


Inoh Y,Furuno T,Hirashima N,Kitamoto D,Nakanishi M




Has Abstract


2011-10-28 00:00:00














  • Nuclear localization signals in the core protein of hepatitis C virus.

    abstract::The core protein of the hepatitis C virus is derived from the N-terminal 191 amino acids of the viral polyprotein by proteolytic cleavage. In the current study, subcellular localizations of the HCV core and its beta-galactosidase fusion proteins in transfected cells were examined by indirect immunofluorescence and cyt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chang SC,Yen JH,Kang HY,Jang MH,Chang MF

    更新日期:1994-12-15 00:00:00

  • Ca2(+)-channel agonist BAY K8644 mimics 1,25(OH)2-vitamin D3 rapid enhancement of Ca2+ transport in chick perfused duodenum.

    abstract::To further understand the molecular mechanism by which 1,25(OH)2-vitamin D3 [1,25(OH2D3] rapidly stimulates intestinal calcium transport (termed "transcaltachia"), the effect of the calcium channel agonist BAY K8644 was studied in vascularly perfused duodenal loops from normal, vitamin D-replete chicks. BAY K8644, 2 m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: de Boland AR,Nemere I,Norman AW

    更新日期:1990-01-15 00:00:00

  • Adrenergic and calcium-mediated subcellular redistribution of protein kinase C in primary neuronal cultures.

    abstract::Incubation of primary neuronal cultures prepared from the brains of neonatal rats with 50 microM epinephrine resulted in the transient redistribution of protein kinase C from the cytosol to the particulate fraction. This effect occurred after 1 and 5 min of incubation and resulted in a decrease in cytosolic protein ki...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kalberg CJ,Sumners C

    更新日期:1990-01-15 00:00:00

  • Partial characterization of guinea pig cholinephosphotransferase cDNA.

    abstract::An alternative molecular biology strategy is needed to characterize cholinephosphotransferase (CPT) gene because of the complexity of the problem associated with the solubilization of the membrane-bound enzyme without denaturation. We have synthesized five heterologous oligonucleotide probes based on the published yea...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Das SK,Beg O,Mukherjee S

    更新日期:1997-12-18 00:00:00

  • A mouse model of mitochondrial complex III dysfunction induced by myxothiazol.

    abstract::Myxothiazol is a respiratory chain complex III (CIII) inhibitor that binds to the ubiquinol oxidation site Qo of CIII. It blocks electron transfer from ubiquinol to cytochrome b and thus inhibits CIII activity. It has been utilized as a tool in studies of respiratory chain function in in vitro and cell culture models....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Davoudi M,Kallijärvi J,Marjavaara S,Kotarsky H,Hansson E,Levéen P,Fellman V

    更新日期:2014-04-18 00:00:00

  • In vitro transfer of phosphatidylcholines and their ether analogs by a human and rat plasma exchange factor.

    abstract::The phospholipid transfer properties of human and rat plasma have been studied using radiolabeled phosphatidylcholines (PCs) and diether PCs as well as a series of fluorescent PCs. The PC transfer activities of human and rat lipoprotein deficient sera are similar. Lipoprotein deficient rat serum transfers PC at a rate...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pownall HJ,Hickson DL,Gotto AM Jr,Massey JB

    更新日期:1984-03-15 00:00:00

  • High salt-diet reduces SLC14A1 gene expression in the choroid plexus of Dahl salt sensitive rats.

    abstract::Elevated Na(+) concentration ([Na(+)]) in the cerebrospinal fluid (CSF) contributes to the development of salt-sensitive hypertension. CSF is formed by the choroid plexus (CP) in cerebral ventricles, and [Na(+)] in CSF is controlled by transporters in CP. Here, we examined the effect of high salt diet on the expressio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Guo L,Meng J,Xuan C,Ge J,Sun W,O'Rourke ST,Sun C

    更新日期:2015-05-29 00:00:00

  • Binding analyses of Cry1Ab and Cry1Ac with membrane vesicles from Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis.

    abstract::The binding properties of Bacillus thuringiensis toxins to brush border membrane vesicles of Dipel-resistant and -susceptible Ostrinia nubilalis larvae were compared using ligand-toxin immunoblot analysis, surface plasmon resonance (SPR), and radiolabeled toxin binding assays. In ligand-toxin immunoblot analysis, the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li H,González-Cabrera J,Oppert B,Ferré J,Higgins RA,Buschman LL,Radke GA,Zhu KY,Huang F

    更新日期:2004-10-08 00:00:00

  • Cloning and sequencing of human LH/hCG receptor cDNA.

    abstract::We have isolated and sequenced a cDNA encoding the human luteinizing hormone--choriogonadotropin (LH/hCG) receptor. The deduced amino acid sequence (699 residues) containing seven putative transmembrane segments displays sequence similarity to G protein-coupled receptors. The receptor consists of 335 residue extracell...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Minegishi T,Nakamura K,Takakura Y,Miyamoto K,Hasegawa Y,Ibuki Y,Igarashi M,Minegish T corrected to Minegishi T

    更新日期:1990-11-15 00:00:00

  • Affinity modification of NADPH-cytochrome P-450 reductase.

    abstract::The active centre of NADPH-cytochrome P-450 reductase contains the lysine residue essential for catalytic activity. Chemical modification of epsilon-amino group of this lysine residue is the subject of the present study. To modify the epsilon-amino group, we have employed the periodate-oxidized NADP+ and NAD+ (o-NAD(P...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Slepneva IA,Weiner LM

    更新日期:1988-09-15 00:00:00

  • Tissue distribution and characterization of prorenin-converting enzyme in mouse.

    abstract::Renin is produced from an inactive precursor, prorenin, through endoproteolytic cleavage at paired basic amino-acid residues. Using (35S)methionine-labeled prorenin, that was synthesized with Xenopus oocyte expression system, as a substrate, we have determined the tissue distribution and the nature of prorenin-convert...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nakayama K,Kim WS,Hatsuzawa K,Hashiba K,Murakami K

    更新日期:1989-01-31 00:00:00

  • Isolation and characterization of a novel ribosome-inactivating protein, beta-kirilowin, from the seeds of Trichosanthes kirilowii.

    abstract::A novel ribosome-inactivating protein designated beta-kirilowin was isolated from the seeds of Trichosanthes kirilowii. The proteins exhibited strong abortifacient activity in pregnant mice and produced a single band after SDS-polyacrylamide gel electrophoresis with an apparent mol. wt. of 27,500 daltons. It was activ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dong TX,Ng TB,Yeung HW,Wong RN

    更新日期:1994-02-28 00:00:00

  • MicroRNA-215 suppresses cell proliferation, migration and invasion of colon cancer by repressing Yin-Yang 1.

    abstract::Colorectal cancer is one of the most common malignant tumors worldwide with rising incidence. MicroRNAs are small non-coding RNAs that implicate in multiple physiological or pathological processes. The aberrant expression of miRNA-215 (miR-215) has been illustrated in various types of cancers. However, the expression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen Z,Han S,Huang W,Wu J,Liu Y,Cai S,He Y,Wu S,Song W

    更新日期:2016-10-21 00:00:00

  • Dioxin interferes in chromosomal positioning through the aryl hydrocarbon receptor.

    abstract::Each chromosome occupies its own-specific space called a 'territory' within the interphase nucleus, and the arrangement of chromosome territories (CTs) is important in epigenetic mechanisms. The molecular mechanism to determine the positioning of CTs, however, remains unknown. On the other hand, dioxin is known to be ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Oikawa K,Yoshida K,Takanashi M,Tanabe H,Kiyuna T,Ogura M,Saito A,Umezawa A,Kuroda M

    更新日期:2008-09-19 00:00:00

  • Disruption of the mu-delta opioid receptor heteromer.

    abstract::The crystal structure of the mu and kappa opioid receptors has revealed dimeric structural arrangements. Mu-delta receptors heteromers also exist and we have identified discrete cytoplasmic regions in each receptor required for oligomer formation. In the carboxyl tail of the delta receptor we identified three glycine ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: O'Dowd BF,Ji X,O'Dowd PB,Nguyen T,George SR

    更新日期:2012-06-15 00:00:00

  • Acetylation and level of mitochondrial transcription factor A in several organs of young and old rats.

    abstract::To gain further information on the role of mitochondrial transcription factor A (TFAM) in mitochondrial biogenesis, we studied the post-translational modifications of the protein in 6- and 28-month-old rat liver. Mass spectrometry and immunoblot analysis revealed that TFAM was acetylated at a single lysine residue and...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dinardo MM,Musicco C,Fracasso F,Milella F,Gadaleta MN,Gadaleta G,Cantatore P

    更新日期:2003-01-31 00:00:00

  • Biotin enhances ATP synthesis in pancreatic islets of the rat, resulting in reinforcement of glucose-induced insulin secretion.

    abstract::Previous studies showed that biotin enhanced glucose-induced insulin secretion. Changes in the cytosolic ATP/ADP ratio in the pancreatic islets participate in the regulation of insulin secretion by glucose. In the present study we investigated whether biotin regulates the cytosolic ATP/ADP ratio in glucose-stimulated ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sone H,Sasaki Y,Komai M,Toyomizu M,Kagawa Y,Furukawa Y

    更新日期:2004-02-13 00:00:00

  • Impaired IL-2 expression in latent HIV-1 infection.

    abstract::Regarding the T cell function in HIV-1 infection, activation of T cells is enhanced in acutely HIV-1-infected T cells upon stimuli. However, T cell immune responses underlying the activation of T cell receptor (TCR) signaling molecules and interleukin (IL)-2 production in latently HIV-1-infected cells are poorly under...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shin Y,Yoon CH,Lim H,Park J,Roh TY,Kang C,Choi BS

    更新日期:2015-08-07 00:00:00

  • Bifunctional DNA-gold nanoparticle conjugates as building blocks for the self-assembly of cross-linked particle layers.

    abstract::The DNA-directed self-assembly of surface-bound layers of gold nanoparticles offers a broad range of applications in biomedical analyses as well as in materials science. We here describe a new concept for the assembly of substrate-bound nanoparticle monolayers which employs bifunctional nanoparticles as building block...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Niemeyer CM,Ceyhan B,Noyong M,Simon U

    更新日期:2003-11-28 00:00:00

  • Chimeric caspase molecules with potent cell killing activity in apoptosis-resistant cells.

    abstract::Cellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shearwin-Whyatt L,Baliga B,Doumanis J,Kumar S

    更新日期:2001-04-20 00:00:00

  • Potent ectopic bone-inducing activity of bone morphogenetic protein-4/7 heterodimer.

    abstract::We have purified and characterized recombinant Xenopus bone morphogenetic proteins (xBMPs): homodimers of xBMP-4, 7 and heterodimers (xBMP-4/7) produced by a baculovirus expression system. Highly purified xBMPs had homogeneous NH2-termini predicted from a consensus motif, Arg-X-X-Arg, while they possessed diverse suga...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Aono A,Hazama M,Notoya K,Taketomi S,Yamasaki H,Tsukuda R,Sasaki S,Fujisawa Y

    更新日期:1995-05-25 00:00:00

  • Contribution of a putative salt bridge and backbone dynamics in the structural instability of human prion protein upon R208H mutation.

    abstract::Molecular dynamics simulation method is used to assess the contribution of a disease-associated salt bridge in the early stages of the conformational rearrangement of human prion protein upon Arg208-->His mutation, which causes Creutzfeldt-Jakob disease. Previous investigations have suggested that the breakage of this...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bamdad K,Naderi-Manesh H

    更新日期:2007-12-28 00:00:00

  • CRY2 is suppressed by FOXM1 mediated promoter hypermethylation in breast cancer.

    abstract::Loss of CRY2 confers aggressive phenotypes to breast cancer. However, the mechanism of its downregulation and its prognostic value in breast cancer are still not clear. Our data mining in TCGA breast cancer cohort (TCGA-BRCA) showed that the luminal A subtype of breast cancer had the highest CRY2 expression, while the...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu L,Shen H,Wang Y

    更新日期:2017-08-12 00:00:00

  • Effects of enzymes on elastic modulus of low-density lipoproteins were investigated using atomic force microscopy.

    abstract::Oxidation of low-density lipoproteins (LDLs) induces development of cardiovascular disease. Recently, reports of studies using atomic force microscopy (AFM) have described that the elastic modulus of metal-induced oxidized LDLs is lower than the modulus before oxidation. However, the mechanisms of change of the elasti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takeda S,Sakurai T,Hui SP,Fuda H,Chiba H

    更新日期:2018-06-27 00:00:00

  • Properties and secondary structure analysis of BanI endonuclease: identification of putative active site.

    abstract::Biochemical properties of Type II restriction enzyme BanI were characterized. Kinetic parameters were evaluated and an enhancement of rate was observed when the recognition site was located in a more central position in the substrate, suggesting that BanI locates its recognition site by a sliding mechanism. As BanI ha...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Advani S,Roy KB

    更新日期:2000-12-09 00:00:00

  • The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum.

    abstract::Ovarian progesterone secretion during the diestrus stage of the estrous cycle is produced by luteal cells derived from granulosa and thecal cells after the differentiation process that follows ovulation. Our results show that blockade of the preovulatory rise of ovarian ornithine decarboxylase (ODC), a key enzyme in p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bastida CM,Tejada F,Cremades A,Peñafiel R

    更新日期:2002-04-26 00:00:00

  • Polypyrimidine tract-binding protein influences negative strand RNA synthesis of dengue virus.

    abstract::Flavivirus non-structural protein 4A (NS4A) induces membrane rearrangements to form viral replication complex and functions as interferon antagonist. However, other non-structural roles of NS4A protein in relation to virus life-cycle are poorly defined. This study elucidated if dengue virus (DENV) NS4A protein interac...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jiang L,Yao H,Duan X,Lu X,Liu Y

    更新日期:2009-07-24 00:00:00

  • The importance of the hydroxyl moieties for inhibition of the Ca(2+)-ATPase by trilobolide and 2,5-di(tert-butyl)-1,4-benzohydroquinone.

    abstract::Trilobolide and 2,5-di(tert-butyl)-1,4-benzohydroquinone (BHQ) are potent inhibitors of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum. Desoxytrilobolide and 2,5-di(tert-butyl)-1,4-diacetylphenol (acetyl-BHQ) have much lower potencies than their parent compounds and 2,5-di(tert-butyl)-1,4-benzoquinone (BQ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wictome M,Holub M,East JM,Lee AG

    更新日期:1994-03-15 00:00:00

  • 1,2-Naphthoquinone disrupts the function of cAMP response element-binding protein through covalent modification.

    abstract::1,2-Naphthoquinone (1,2-NQ) is an atmospheric contaminant with electrophilic properties that allow it to react readily with protein thiol groups such as those found on the cAMP response element-binding protein (CREB), a transcription factor with conserved cysteine residues that regulate DNA binding. In the present stu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Endo A,Sumi D,Kumagai Y

    更新日期:2007-09-14 00:00:00

  • Expression of the ED B fibronectin isoform in adult human articular cartilage.

    abstract::The most recently described region of alternate splicing of fibronectin mRNA results in expression of an isoform which includes the type III repeat termed ED B (EIII B). To date this isoform has been detected in transformed cells in culture, in the synovial membrane and ovary but in no other adult tissue, and in embry...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Burton-Wurster N,Lust G,Wert R

    更新日期:1989-12-15 00:00:00