Electrical stimulation induces the level of TGF-beta1 mRNA in osteoblastic cells by a mechanism involving calcium/calmodulin pathway.

abstract：:It has been reported that changes in deoxynucleotide pool levels following exposure to UV might lead to an overestimation of the UV-induced depression in DNA synthesis as analyzed by incorporation of 3H-thymidine (1). We attempted to determine the importance of such pool effects in two ways. First, we examined the gra...

journal_title：Biochemical and biophysical research communications

authors： Griffiths TD,Ling SY

更新日期：1984-09-17 00:00:00

abstract：:We have constructed a highly useful phage-displayed human antibody repertoire with limited cloning efforts. Our strategy was to maximize diversity during the first steps of library construction through the use of various lymphoid sources from several donors, inclusion of different immunoglobulin isotypes, and performa...

journal_title：Biochemical and biophysical research communications

authors： Rojas G,Lamdan H,Padron S,Munoz Y,Ayala M,Gavilondo JV

更新日期：2005-11-04 00:00:00

abstract：BACKGROUND:In our study, we investigated the expression and function of microRNA-29 in myocardial microvascular endothelial cells (MMEVC) in type 2 diabetic Goto-Kakizaki (GK) rats. METHODS:MiR-29 gene expression was compared, by qRT-PCR between diabetic GK rat MMEVC and non-diabetic Wistar rat MMEVC. MiR-29 was downr...

journal_title：Biochemical and biophysical research communications

authors： Li Z,Jiang R,Yue Q,Peng H

更新日期：2017-05-20 00:00:00

abstract：:Adipogenesis is the process of differentiation from preadipocytes to adipocytes and is orchestrated by various transcription factors, such as the peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT-enhancer-binding protein alpha (C/EBPα). Oxidative stress is also a crucial factor in adipogenesis, an...

journal_title：Biochemical and biophysical research communications

authors： Kim BR,Lee GY,Yu H,Maeng HJ,Oh TJ,Kim KM,Moon JH,Lim S,Jang HC,Choi SH

更新日期：2018-03-18 00:00:00

abstract：:The sequence for the 3' portion of amoB, a gene encoding a 43-kDa polypeptide component ammonia monooxygenase of Nitrosomonas europaea, is presented. The derived polypeptide has no homology with other known proteins. AmoA and amoB are the only open reading frames in the putative amo operon. ...

journal_title：Biochemical and biophysical research communications

authors： Bergmann DJ,Hooper AB

更新日期：1994-10-28 00:00:00

abstract：:Several recent studies have suggested that cancer stem cells (CSCs) are involved in resistance to gefitinib in non-small cell lung cancer (NSCLC). Oct4, a member of the POU-domain transcription factor family, has been shown to be involved in CSC properties of various cancers. We previously reported that Oct4 and the p...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi I,Takahashi F,Nurwidya F,Nara T,Hashimoto M,Murakami A,Yagishita S,Tajima K,Hidayat M,Shimada N,Suina K,Yoshioka Y,Sasaki S,Moriyama M,Moriyama H,Takahashi K

更新日期：2016-04-22 00:00:00

abstract：:ATP binding cassette (ABC) transporters have a functional unit formed by two transmembrane domains and two nucleotide binding domains (NBDs). ATP-bound NBDs dimerize in a head-to-tail arrangement, with two nucleotides sandwiched at the dimer interface. Both NBDs contribute residues to each of the two nucleotide-bindin...

journal_title：Biochemical and biophysical research communications

authors： Zoghbi ME,Altenberg GA

更新日期：2014-01-03 00:00:00

abstract：:Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the regulation of gene expression during growth, differentiation, apoptosis, and inflammation. Autoregulation is relatively common in the modulation of C/EBP gene expression and, for the human and murine C/EBPa...

journal_title：Biochemical and biophysical research communications

authors： Davies GE,Sabatakos G,Cryer A,Ramji DP

更新日期：2000-05-10 00:00:00

abstract：:Amphibian skin contains rich bradykinin-related peptides, but the mode of biosynthesis of these peptides is unknown. In the present study, a novel bradykinin-related peptide, termed bombinakinin M, was purified from skin secretions of the Chinese red belly toad Bombina maxima. Its primary sequence was established as D...

journal_title：Biochemical and biophysical research communications

authors： Lai R,Liu H,Hui Lee W,Zhang Y

更新日期：2001-08-17 00:00:00

abstract：:The hyperpolarization-activated cation current I(h) is an important regulator of neuronal excitability and may contribute to the properties of the dentate gyrus granule (DGG) cells, which constitute the input site of the canonical hippocampal circuit. Here, we investigated changes in I(h) in DGG cells in human tempora...

journal_title：Biochemical and biophysical research communications

authors： Surges R,Kukley M,Brewster A,Rüschenschmidt C,Schramm J,Baram TZ,Beck H,Dietrich D

更新日期：2012-03-30 00:00:00

abstract：:Neddylation is an ubiquitin-like modification of proteins that affects the activity, stability and protein-protein interaction of its substrates. Apart from its role as a promoter for Cullin ring E3 ligase to positively regulate the ubiquitylation process, other functional studies about neddylation are still lacking. ...

journal_title：Biochemical and biophysical research communications

authors： Li S,Fang W,Cui Y,Shi H,Chen J,Li L,Zhang L,Zhang X

更新日期：2020-09-03 00:00:00

abstract：:The phthalate ester insensitive blue-green algae (Synechococcus lividus) were used as a food source to extend the survival of synchronously hatched brine shrimp (Artemia salina) larvae allowing measurement of a reduced toxic response to phthalate esters at late post-hatching stages of development. The maximum acute to...

journal_title：Biochemical and biophysical research communications

authors： Acey RA,Bailey S,Healy P,Jo C,Unger TF,Hudson RA

更新日期：2002-12-13 00:00:00

abstract：:Hydroxocobalamin is present in fairly large proportions both in foods and in the human body and apparently plays an important biological role. Since cyanocobalamin seems to play hardly any significant biochemical role in healthy humans, several physicians prefer to administer hydroxocobalamin to vitamin B12 deficient ...

journal_title：Biochemical and biophysical research communications

authors： Kohli RK,Nath A

更新日期：1984-12-14 00:00:00

abstract：:The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/α-NAD(+) c...

journal_title：Biochemical and biophysical research communications

authors： Zheng Y,Ko TP,Yang Y,Shao W,Guo RT

更新日期：2015-01-16 00:00:00

abstract：:A cDNA containing the entire coding sequence for the subunit protein of rat liver class theta glutathione S-transferase (GST) Yrs-Yrs was isolated from a rat liver lambda gt11 cDNA library. The cDNA, designated GST theta-1, consisted of 1,258 bp which had an open reading frame of 732 bp encoding a polypeptide of 244 a...

journal_title：Biochemical and biophysical research communications

authors： Ogura K,Nishiyama T,Okada T,Kajital J,Narihata H,Watabe T,Hiratsuka A,Watabe T

更新日期：1991-12-31 00:00:00

abstract：:Membrane-type 1 matrix metalloproteinase (MT1-MMP) localized on the plasma membrane plays a central role in various normal biological responses including tissue remodeling, wound heeling, and angiogenesis and in cancer cell invasion and metastasis, by functioning as a collagenase and activating other matrix metallopro...

journal_title：Biochemical and biophysical research communications

authors： Miyata T,Ohnishi H,Suzuki J,Yoshikumi Y,Ohno H,Mashima H,Yasuda H,Ishijima T,Osawa H,Satoh K,Sunada K,Kita H,Yamamoto H,Sugano K

更新日期：2004-10-08 00:00:00

abstract：:The Na+/Ca2+ exchanger (NCX) is the primary Ca2+ extrusion mechanism in cardiomyocytes. To further investigate the role of NCX in excitation-contraction coupling and Ca2+ homeostasis, we created murine models with altered expression levels of NCX. Homozygous overexpression of NCX resulted in mild cardiac hypertrophy. ...

journal_title：Biochemical and biophysical research communications

authors： Pott C,Goldhaber JI,Philipson KD

更新日期：2004-10-01 00:00:00

abstract：:Insect development is controlled by the combined actions of ecdysteroid and juvenile hormones. Transcriptional control by ecdysteroid hormones is mediated via two nuclear receptor superfamily members, ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle (USP). Although the ecdysteroid hormone 20-hydrox...

journal_title：Biochemical and biophysical research communications

authors： Maki A,Sawatsubashi S,Ito S,Shirode Y,Suzuki E,Zhao Y,Yamagata K,Kouzmenko A,Takeyama K,Kato S

更新日期：2004-07-16 00:00:00

abstract：:Invadopodia are cellular structures that are thought to mediate tumor invasion. ASAP1, an Arf GTPase-activating protein (GAP) containing a BAR domain, is a substrate of Src. ASAP1 is required for the assembly of invadopodia and podosomes, which are Src-induced structures related to invadopodia in NIH 3T3 fibroblasts. ...

journal_title：Biochemical and biophysical research communications

authors： Shiba Y,Randazzo PA

更新日期：2011-03-25 00:00:00

abstract：:TGF beta is a multifunctional cytokine that regulates alveolar epithelial cells as well as immune cells and fibroblasts. TGF beta inhibits surfactant protein A, B and C expression in fetal human lung and can inhibit type II cell proliferation induced by FGF7 (KGF). However, little is known about direct effects of TGF ...

journal_title：Biochemical and biophysical research communications

authors： Correll KA,Edeen KE,Zemans RL,Redente EF,Mikels-Vigdal A,Mason RJ

更新日期：2018-05-23 00:00:00

abstract：:The murine C-5 cytosine DNA methyltransferase (MTase, E.C.2.1.1.37) containing a hexahistidine affinity leader peptide has been expressed at levels which are at least 50-fold higher than previously reported. The recombinant enzyme has activity levels similar to the wild-type enzyme. The recombinant polypeptide binds t...

journal_title：Biochemical and biophysical research communications

authors： Glickman JF,Reich NO

更新日期：1994-11-15 00:00:00

abstract：:Plucked (pk) is an autosomal recessive mouse mutation with a hair phenotype that arose spontaneously in the DBA/2J strain. Histological studies indicate that adult pk mutant mice lose truncal hair because of the scarring of follicles due to an apparent obstruction of the outward movement of the hair shaft within the f...

journal_title：Biochemical and biophysical research communications

authors： Luo J,Zhang L,Stenn K,Prouty S,Parimoo S

更新日期：2005-02-04 00:00:00

abstract：:Wound healing in ligaments is a complex process which leads to functionally impaired scar tissue, even after extended time postinjury. To investigate the potential role of proteinases and inhibitors, as well as potential regulators of their expression, mRNA levels for collagenase, stromelysin, urokinase, PAI-1, and TI...

journal_title：Biochemical and biophysical research communications

authors： Reno C,Boykiw R,Martinez ML,Hart DA

更新日期：1998-11-27 00:00:00

abstract：:RT-PCR, a rapidly emerging technique for the detection of RNA, is being used by many investigators to quantify small amounts of RNA. Accurate quantification of RNA content has been facilitated by the use of competitive amplicons as internal controls. We demonstrate that losses in sensitivity and accuracy are associate...

journal_title：Biochemical and biophysical research communications

authors： Henley WN,Schuebel KE,Nielsen DA

更新日期：1996-09-04 00:00:00

abstract：:We prepared a cleavage site-directed antiserum against Caspase-3 (anti-p20/17), which reacts with the p20/17 fragment (p20/17) activated by cleavage but not proCaspase-3 (p32), and examined the relationship between the activation of Caspase-3 and apoptosis. We identified p20/17-positive cells where cell death occurs n...

journal_title：Biochemical and biophysical research communications

authors： Kouroku Y,Urase K,Fujita E,Isahara K,Ohsawa Y,Uchiyama Y,Momoi MY,Momoi T

更新日期：1998-06-29 00:00:00

abstract：:Dinoflagellates are protists with permanently condensed chromosomes that lack histones and whose nuclear membrane remains intact during mitosis. These unusual nuclear characters have suggested that the typical cell cycle regulators might be slightly different than those in more typical eukaryotes. To test this, a cycl...

journal_title：Biochemical and biophysical research communications

authors： Bertomeu T,Morse D

更新日期：2004-10-29 00:00:00

abstract：:In AL-amyloidosis the cause of amyloid fibril formation in beta-pleated sheets from the precursor protein immunoglobulin light chain is not established, but studies of AL-proteins indicate that amino acid substitutions are important in the pathogenesis. Amyloid material was extracted from a subcutaneous fat tissue bio...

journal_title：Biochemical and biophysical research communications

authors： Olsen KE,Sletten K,Westermark P

更新日期：1998-04-28 00:00:00

abstract：:Although proteins are generally composed of l-alpha-amino acids, biologically uncommon D-beta-aspartic acid (Asp)-containing proteins have been reported in various tissues from elderly individuals. Our previous study indicated that the N/N1003A cell line, derived from rabbit lens, includes D-beta-Asp-containing protei...

journal_title：Biochemical and biophysical research communications

authors： Takata T,Shimo-Oka T,Miki K,Fujii N

更新日期：2005-09-09 00:00:00

abstract：:As a model host, the nematode Caenorhabditis elegans has been used for studying unknown pathogen-host interactions and identifying novel virulence factors in bacterial pathogens. Among the bacterial pathogens that can induce death of C. elegans is enterohemorrhagic Escherichia coli (EHEC) O157:H7, a major serotype of ...

journal_title：Biochemical and biophysical research communications

authors： Youn M,Lee KM,Kim SH,Lim J,Yoon JW,Park S

更新日期：2013-07-05 00:00:00

abstract：BACKGROUND AND AIMS:Concanavalin A (Con A) activates T lymphocytes and induces CD4+ T cell-mediated hepatic injury in mice. Pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), and interleukin-6 (IL-6), are critical mediators in this experimental model. Activation o...

journal_title：Biochemical and biophysical research communications

authors： Odashima M,Otaka M,Jin M,Horikawa Y,Matsuhashi T,Ohba R,Linden J,Watanabe S

更新日期：2006-09-08 00:00:00