Tityus discrepans scorpion venom activates platelets through GPVI and a novel Src-dependent signaling pathway.


:In humans and other mammals, Tityus discrepans (Td) scorpion envenomation produces a variety of systemic effects including respiratory distress, a generalized inflammatory reaction, modulation of blood pressure, fibrin formation, and platelet activation. For many of these effects, the venom components and underlying mechanisms are not known. In the present study, we demonstrate that Td venom (TdV) stimulates integrin αIIbβ3-dependent aggregation of washed human and mouse platelets downstream of Src kinase activation. The pattern of increase in tyrosine phosphorylation induced by TdV in human platelets is similar to that induced by the collagen receptor GPVI, and includes FcR γ-chain, Syk, and PLC γ 2. Confirmation of GPVI activation by TdV was achieved by expression of human GPVI in chicken DT40 B cells and use of a reporter assay. To our surprise, TdV was able to activate mouse platelets deficient in the GPVI-FcR γ-chain complex through a pathway that was also dependent on Src kinases. TdV therefore activates platelets through GPVI and a second, as yet unidentified Src kinase-dependent pathway.






Brazón J,Hughes CE,Mori J,Sevcik C,D'suze G,Watson SP




Has Abstract


2011-01-01 00:00:00












  • Platelet aggregation in erythropoietin treated dialysis patients.

    abstract::Recombinant human erythropoietin is a successful treatment for the anaemia of renal failure. Its use has however been associated with thromboembolic complications. Changes in platelet number and behaviour in vitro were studied in: (1) 23 haemodialysis patients, and 10 continuous ambulatory peritoneal dialysis patients...


    pub_type: 杂志文章


    authors: Taylor JE,Henderson IS,Stewart WK,Belch JJ

    更新日期:1992-01-01 00:00:00

  • Hyperthermia induces platelet apoptosis and glycoprotein Ibalpha ectodomain shedding.

    abstract::Hemorrhage is a significant pathological feature of some fever or hyperthermia-related diseases, such as dengue fever and heatstroke. Although the mechanisms of hemorrhage in these diseases are thought to be complex, whether there is an association between hemorrhage and hyperthermia or fever remains unclear. Platelet...


    pub_type: 杂志文章


    authors: Wang Z,Shi Q,Li S,Du J,Liu J,Dai K

    更新日期:2010-01-01 00:00:00

  • Genetic polymorphisms of human platelet antigens in Euro-African and Japanese descendants from Parana, Southern Brazil.

    abstract::The frequency distributions of HPA-1 to HPA-6 and HPA-15 were evaluated in two Brazilian populations from Parana: a mixed population of predominantly Caucasians and a population of Japanese descendants. Genotyping was performed by PCR-SSP in 364 unrelated individuals. Differences in the distribution of HPA highlight d...


    pub_type: 杂志文章


    authors: Silvestre APA,Zacarias JMV,Guelsin GAS,Visentainer JEL,Sell AM

    更新日期:2017-09-01 00:00:00

  • Response of blood platelets to resveratrol.

    abstract::The phytochemical resveratrol (3,4',5-trihydroxystilbene), which is found in grapes and wine, has been reported to have a variety of anti-inflammatory, anti-platelet and anti-carcinogenic effects. The aim of the present study was to evaluate the effects of trans-resveratrol on adhesion of blood platelets to collagen, ...


    pub_type: 杂志文章


    authors: Zbikowska HM,Olas B,Wachowicz B,Krajewski T

    更新日期:1999-07-01 00:00:00

  • A new variant of Glanzmann's thrombasthenia with defective activation-dependent fibrinogen binding and altered expression of epitopes for several monoclonal antibodies against GP IIb-IIIa.

    abstract::In a family, a moderate bleeding disorder in two patients has been specified as Glanzmann's thrombasthenia because of characteristic defects in platelet function. Analysis of platelet membrane glycoproteins revealed about a 50% decrease in the amount of GP IIb-IIIa complex (α(11b)β(3) integrin), which appeared normal ...


    pub_type: 杂志文章


    authors: Meyer M,Thieme A,Jablonka B,Just M,Ströhl C,Schellenberg I,Kirchmaier CM

    更新日期:1996-01-01 00:00:00

  • Tetraspanin-Fc receptor interactions.

    abstract::The tetraspanins are a superfamily of membrane glycoproteins which facilitate the interaction of membrane and intracellular signalling molecules (e.g., integrins, pro-growth factors and their receptors, protein kinase-C) in the formation of membrane signalling microdomains (sometimes referred to as the tetraspanin web...


    pub_type: 杂志文章,评审


    authors: Moseley GW

    更新日期:2005-02-01 00:00:00

  • Profile of reticulated platelets in the early, subacute and late phases after transient ischemic attack or ischemic stroke.

    abstract::Information regarding the profile of reticulated platelets (RP) in ischemic cerebrovascular disease (CVD) patients is limited. Data from two prospective, observational, case-control studies were combined to compare the %RP using whole blood flow cytometry in patients ≤ 4 weeks of TIA/stroke onset (baseline, N = 210), ...


    pub_type: 杂志文章


    authors: Lim ST,Tobin WO,Murphy S,Kinsella JA,Smith DR,Lim SY,Murphy SM,Coughlan T,Collins DR,O'Neill D,Egan B,Tierney S,McCabe D

    更新日期:2020-12-21 00:00:00

  • Influence of the alpha-adrenoreceptor naftopidil and doxazosin, on adrenaline-induced serotonin platelets: comparison with the antagonists, collagen and efflux by human effects of nifedipine.

    abstract::Collagen (5 microg/ml) stimulation of washed platelets increased endogenous serotonin (5-HT) release to the medium from 13.88 1.39 to 188.67 26.37 pmol/108 platelets ( P < 0.001). Adrenaline (16 microM) also increased 5-HT release, from 11.0 1.46 to 110.6 29.9 pmol/108 platelets ( P < 0.02). Naftopidil enhanced collag...


    pub_type: 杂志文章


    authors: Alarayyed NA,Prichard BN,Betteridge DJ,Smith CC

    更新日期:1997-01-01 00:00:00

  • Use of a platelet filter test in patients with thrombocytosis.

    abstract::In the differential diagnosis of primary and secondary thrombocytosis platelet function tests may play an important role. We examined the applicability of a platelet filter test (shear-dependent platelet aggregation) as a tool, to differentiate primary thrombocytosis (cases with myeloproliferative disorders) from seco...


    pub_type: 杂志文章


    authors: Rejtö L,Schlammadinger A,László P,Kiss A,Telek B,Boda Z

    更新日期:2000-02-01 00:00:00

  • High shear induces platelet dysfunction leading to enhanced thrombotic propensity and diminished hemostatic capacity.

    abstract::Thrombosis and bleeding are devastating adverse events in patients supported with blood-contacting medical devices (BCMDs). In this study, we delineated that high non-physiological shear stress (NPSS) caused platelet dysfunction that may contribute to both thrombosis and bleeding. Human blood was subjected to NPSS wit...


    pub_type: 杂志文章


    authors: Chen Z,Mondal NK,Zheng S,Koenig SC,Slaughter MS,Griffith BP,Wu ZJ

    更新日期:2019-01-01 00:00:00

  • Safety and efficacy of IIb/IIIa inhibitors in combination with highly active oral antiplatelet regimens in acute coronary syndromes: A meta-analysis of pivotal trials.

    abstract::The risk and benefit of GP-IIb/IIIa Inhibition (GPI) in combination with recent antiplatelet regimens in acute coronary syndromes (ACS) remain unassessed. The advent of fast-acting highly active oral P2Y12 inhibitors questions the additional value and risk of their association with GPI. We studied the effect of GPI in...


    pub_type: 杂志文章,meta分析,评审


    authors: Roule V,Agueznai M,Sabatier R,Blanchart K,Lemaître A,Ardouin P,Collet JP,Milliez P,Montalescot G,Beygui F

    更新日期:2017-03-01 00:00:00

  • Comparative anti-platelet and antioxidant properties of polyphenol-rich extracts from: berries of Aronia melanocarpa, seeds of grape and bark of Yucca schidigera in vitro.

    abstract::The aim of the present study was to investigate and compare the anti-platelet action of extracts from three different plants: bark of Yucca schidigera, seeds of grape and berries of Aronia melanocarpa (chokeberry). Anti-platelet action of tested extracts was compared with action of well characterized antioxidative and...


    pub_type: 杂志文章


    authors: Olas B,Wachowicz B,Tomczak A,Erler J,Stochmal A,Oleszek W

    更新日期:2008-02-01 00:00:00

  • Expression of ADP receptor P2Y12, thromboxane A2 receptor and C-type lectin-like receptor 2 in cord blood-derived megakaryopoiesis.

    abstract::The ADP receptor P2Y12, the thromboxane A2 receptor (TXA2R) and the C-type lectin-like receptor 2 (CLEC-2) mediate platelet activation by different mechanisms. Only little is known about the expression of the receptors in human megakaryopoiesis. Our study aimed to establish a flow cytometry (FC) method for the measure...


    pub_type: 杂志文章


    authors: Gerhards C,Uhlig S,Etemad M,Christodoulou F,Bieback K,Klüter H,Bugert P

    更新日期:2020-07-03 00:00:00

  • Platelet count and mortality of H7N9 infected patients in Guangdong, China.

    abstract::Avian influenza A (H7N9) is a serve zoonosis with a high mortality rate. Timely and effective diagnosis and early warning is crucial for the clinical treatment of H7N9 patients. The previous studies indicated that thrombocytopenia was associated with the prognosis of influenza cases, but the related evidence of platel...


    pub_type: 杂志文章


    authors: Chen Y,Yang Y,Cheng J,Lu J,Hu W

    更新日期:2020-01-01 00:00:00

  • A comparative study of antioxidative activity of calcium-D-glucarate, sodium-D-gluconate and D-glucono-1,4-lactone in a human blood platelet model.

    abstract::D-glucono-1,4-lactone, sodium D-gluconate and calcium D-glucarate are non-toxic glucose derivatives occurring naturally in fruits and vegetables. Calcium D-glucarate is promoted as an orally bioavailability dietary supplement with potential chemopreventive activity without adverse effects. Despite many commercial appl...


    pub_type: 杂志文章


    authors: Saluk-Juszczak J

    更新日期:2010-01-01 00:00:00

  • Mean platelet volume and eosinophilia relationship in patients with bullous pemphigoid.

    abstract:INTRODUCTION:Bullous pemphigoid (BP) is an autoimmune skin disease that is characterized by the formation of blisters on normal or erythematous skin. In patients with BP, eosinophils are highly represented in the inflammatory infiltrate of the lesional skin and their levels are often increased in peripheral blood. It i...


    pub_type: 杂志文章


    authors: Rifaioglu EN,Sen BB,Ekiz Ö,Dogramaci AC

    更新日期:2014-01-01 00:00:00

  • Investigation of platelet function and platelet disorders using flow cytometry.

    abstract::Patients with thrombocytopenia or platelet disorders are at risk of severe bleeding. We report the development and validation of flow cytometry assays to diagnose platelet disorders and to assess platelet function independently of platelet count. The assays were developed to measure glycoprotein levels (panel 1) and p...


    pub_type: 杂志文章


    authors: Rubak P,Nissen PH,Kristensen SD,Hvas AM

    更新日期:2016-01-01 00:00:00

  • Application of microfluidic devices in studies of thrombosis and hemostasis.

    abstract::Due to the importance of fluid flow during thrombotic episodes, it is quite appropriate to study clotting and bleeding processes in devices that have well-defined fluid shear environments. Two common devices for applying these defined shear stresses include the cone-and-plate viscometer and parallel-plate flow chamber...


    pub_type: 杂志文章,评审


    authors: Zhang C,Neelamegham S

    更新日期:2017-07-01 00:00:00

  • Flow cytometry as a tool in the diagnosis of Bernard-Soulier syndrome in Brazilian patients.

    abstract::Bernard-Soulier Syndrome (BSS) is an inherited recessive bleeding disorder. In some instances, diagnosis might be restricted to routine blood exams, including bleeding time, prothrombin time (PT), and partial thromboplastin time (APTT). Exams such as platelet aggregation, and testing for expression of ristocetin cofac...


    pub_type: 杂志文章


    authors: Beltrame MP,Malvezzi M,Zanis J,Pasquini R

    更新日期:2009-06-01 00:00:00

  • An unusual presentation of gestational thrombocytopenia.

    abstract::Thrombocytopenia is classically defined as a platelet count of less than 150 000/µl. Counts from 100 000 to 150 000/µl are considered mildly depressed, 50 000 to 100 000/µl moderately depressed, and less than 50 000/µl severely depressed. Thrombocytopenia occurs in about 10% of pregnant women. Gestational thrombocytop...


    pub_type: 杂志文章


    authors: Lim CC,Patel DK,Bakhtiari A,Subrayan V

    更新日期:2013-01-01 00:00:00

  • Deaggregation is an integral component of the response of platelets to ADP in vitro: kinetic studies of literature and original data.

    abstract::Adenosine diphosphate (ADP) is recognized as an important mediator of platelet aggregation. Transient aggregation at low (< or =1 microM), and sustained aggregation at higher ADP concentrations are consistently observed. Dissociation of platelet aggregates has been described and may explain the reversible component of...


    pub_type: 杂志文章


    authors: Maayani S,Tagliente TM,Schwarz T,Craddock-Royal B,Alcala C,Marrero G,Martinez R

    更新日期:2001-08-01 00:00:00

  • Influence of platelet count and activity on thromboelastography parameters.

    abstract::It has been suggested that thromboelastography TEG) can help in limiting or directing the appropriate use of blood products during surgery. However, the contribution of platelets to the TEG profile has not been studied in detail. Blood was taken from eight healthy subjects and eight patients with peripheral arterial d...


    pub_type: 杂志文章


    authors: Bowbrick VA,Mikhailidis DP,Stansby G

    更新日期:2003-06-01 00:00:00

  • Recovery of platelet reactivity following cessation of either aspirin or ticagrelor in patients treated with dual antiplatelet therapy following percutaneous coronary intervention: a GLOBAL LEADERS substudy.

    abstract::Cessation of one component of dual antiplatelet therapy (DAPT) following percutaneous coronary intervention (PCI) has been associated with increased risk of ischemic events but it is uncertain whether discontinuation of aspirin is preferable to discontinuation of the oral P2Y12 inhibitor. The GLOBAL LEADERS study comp...


    pub_type: 杂志文章


    authors: Hennigan BW,Good R,Adamson C,Parker WAE,Martin L,Anderson L,Campbell M,Serruys PW,Storey RF,Oldroyd KG

    更新日期:2020-12-26 00:00:00

  • Predicting metabolic syndrome by using hematogram models in elderly women.

    abstract:BACKGROUND:Low-grade inflammatory status was thought to be a major underlying mechanism in MetS. White blood cell (WBC) count was one of the inflammatory markers identified to be associated with MetS. Moreover, not only WBC but also hemoglobin (Hb) and platelet (PLT) were all associated with MetS. OBJECTIVE:In this st...


    pub_type: 杂志文章


    authors: Liu H,Hsu CH,Lin JD,Hsieh CH,Lian WC,Wu CZ,Pei D,Chen YL

    更新日期:2014-01-01 00:00:00

  • Platelet-rich plasma in combination with bovine derived xenograft in the treatment of generalized aggressive periodontitis: a case report with re-entry.

    abstract::Prominence has been given recently to the use of platelet-rich plasma (PRP) in combination with bone graft materials for predictably obtaining periodontal regeneration through bioengineering. The purpose of this report was to present clinical, radiographic and re-entry results of a generalized aggressive periodontitis...


    pub_type: 杂志文章


    authors: Yilmaz S,Cakar G,Kuru BE,Yildirim B

    更新日期:2007-11-01 00:00:00

  • EDTA induced changes in platelet structure and function: influence on particle uptake.

    abstract::Ethylenediaminetetraacetic acid (EDTA) causes structural, biochemical and functional damage to blood platelets. The alterations induced are considered irreversible. However, the degree of irreversibility, and whether all functions are similarly compromised by EDTA have not been fully evaluated. The present study has e...


    pub_type: 杂志文章


    authors: White JG,Krumwiede MD,Escolar G

    更新日期:1999-01-01 00:00:00

  • Release of Choline Metabolites from Human Platelets: Evidence for Activation of Phospholipase D and of Phosphatidylcholine-specific Phospholipase C.

    abstract::In aspirin-treated platelets labelled by preincubation with [(3)H]-choline, enhanced release of both [(3)H]-choline and [(3)H]-choline phosphate resulted from stimulation by collagen or thrombin. No such release accompanied stimulation by ADP, platelet-activating factor or adrenaline. Release of [(3)H]-choline phospha...


    pub_type: 杂志文章


    authors: Petty AC,Scrutton MC

    更新日期:1993-01-01 00:00:00

  • Comparison of biological activity of phenolic fraction from roots of Alhagi maurorum with properties of commercial phenolic extracts and resveratrol.

    abstract::Phenolic compounds have different biological properties, including antioxidative activities, but they may also be prooxidants. The effect of phenolic fraction from roots of Alhagi maurorum on oxidative protein/lipid damages (determined by such parameters as levels of protein thiol groups and the concentration of thiob...


    pub_type: 杂志文章


    authors: Olas B,Hamed AI,Oleszek W,Stochmal A

    更新日期:2015-01-01 00:00:00

  • Platelet function monitoring in patients on clopidogrel: What should we learn from GRAVITAS?

    abstract::Combined inhibition of platelet aggregation is essential to prevent recurrent ischemic episodes in patients with acute coronary syndromes and after percutaneous coronary interventions (PCIs). In combination with aspirin, the ADP receptor antagonist clopidogrel is used widespread for this purpose; however, platelet rea...


    pub_type: 杂志文章,评审


    authors: Aradi D,Komócsi A

    更新日期:2012-01-01 00:00:00

  • Blood platelet activation evaluated by flow cytometry: optimised methods for clinical studies.

    abstract::A variety of flow cytometry techniques are in use to evaluate in vivo blood platelet activation. We have in this study further developed and optimised these methods to be suitable for use in clinical studies. By preloading the Monovette EDTA vacuum blood sampling tubes with 1/8 vol 4% (w/v) paraformaldehyde (PFA), we ...


    pub_type: 杂志文章


    authors: Hagberg IA,Lyberg T

    更新日期:2000-05-01 00:00:00