Abstract:
:We have explored both the independent and combined effects of aspirin on cultured endothelial cells and platelets, and its influence on platelet deposition onto an extracellular matrix. Blood was circulated through a flat perfusion chamber with two coverslips placed sequentially with respect to blood flow. The first coverslip (upstream) was covered with a cultured endothelial cell monolayer, and the second (downstream) coated with extracellular matrix obtained after endothelial cell removal. Platelet interaction was measured on the second coverslip. Treatment of endothelial cells on the first coverslip with 100 μM aspirin strongly reduced 6-keto-PGF(1a) levels recovered in the perfusates (118.3 ± 35.8 vs 1038.0 ± 308.5 pg/ml) and significantly increased platelet deposition on the downstream coverslip (% covered surface: 38.6 ± 6.4% vs 14.6 ± 1.8%; P < 0.001). Increased platelet deposition (% covered surface: 24.9 f 3.1%; P < 0.01) was observed in perfusions performed with blood containing aspirinized platelets, in the presence of intact endothelial cells. Treatment with aspirin of both platelets and endothelial cells had no additional effect on platelet adherence. Pretreatment of cultured endothelial cells with aspirin did not influence the adhesive properties of their underlying extracellular matrix. Our results indicate that, although endothelial cell cyclooxygenase is important in regulating platelet adhesion, its blockade seemed to have minimal effect on platelet deposition once platelet-cyclooxygenase was already inhibited.
journal_name
Plateletsjournal_title
Plateletsauthors
Alemany M,Hernández MR,Bozzo J,Bono A,Escolar G,Ordinas A,Bastida Edoi
10.3109/09537109609079506subject
Has Abstractpub_date
1996-01-01 00:00:00pages
29-34issue
1-2eissn
0953-7104issn
1369-1635journal_volume
7pub_type
杂志文章相关文献
PLATELETS文献大全abstract::Stripping of cytoplasm from circulating megakaryocytes (MKs) in the pulmonary capillary bed implicates the lungs as an important site for platelet production in normal physiology. The placenta has been proposed as an alternative location for intrauterine platelet production as fetal lungs are essentially non-functioni...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109209013185
更新日期:1992-01-01 00:00:00
abstract::We have investigated interactions between human blood platelets and the vitronectin-containing fluid-phase terminal complement complex, the SC5b-9, which is a non-cytolytic variant of the membrane attack complex C%9(m). SC5b-9 affinity for the platelet membrane glycoprotein IIb/IIIa (GPIIb/IIIa) complex was demonstrat...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509013269
更新日期:1995-01-01 00:00:00
abstract::Recent studies have indicated that medical options without splenectomy, such as rituximab (RTX) or thrombopoietin receptor agonists (TPO-RAs), can be effective to treat persistent or chronic primary immune thrombocytopenia (ITP). However, it remains to be determined which of these strategies should be the first choice...
journal_title:Platelets
pub_type: 杂志文章,meta分析
doi:10.1080/09537104.2018.1543864
更新日期:2019-01-01 00:00:00
abstract::Strenuous physical exercise leads to platelet activation that is normally counterbalanced by the production of endothelium-derived anti-platelet mediators, including prostacyclin (PGI2) and nitric oxide (NO). However, in the case of endothelial dysfunction, e.g. in atherosclerosis, there exists an increased risk for i...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1254764
更新日期:2017-11-01 00:00:00
abstract::Activated platelets serve as a catalyst for thrombin generation and a source of vasoactive and mitogenic factors affecting vascular remodeling. Oral menopausal hormone treatments (MHT) may carry greater thrombotic risk than transdermal products. This study compared effects of oral and transdermal MHT on platelet chara...
journal_title:Platelets
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3109/09537104.2015.1023273
更新日期:2016-01-01 00:00:00
abstract::To study the possibility of using thrombin generation tests in platelet-rich and platelet-poor plasma for evaluation of dual antiplatelet therapy efficacy in patients with coronary artery disease (CAD), following percutaneous coronary intervention. Venous blood was analyzed from CAD patients aged 53-75 years who had u...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1294680
更新日期:2018-03-01 00:00:00
abstract::We prospectively investigated the prevalence of Helicobacter pylori (H. pylori) infection in a cohort of 54 adult Serbian patients with chronic idiopathic thrombocytopenic purpura (ITP), and examined the effects of its eradication on their platelet counts. H. pylori infection was diagnosed in 39/54 (72.2%) patients, u...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500462487
更新日期:2006-06-01 00:00:00
abstract::Familial bleeding problems are frequently difficult to diagnose because currently used clinical tests cannot identify intracellular molecular defects of platelets. Using platelet proteomics, a comprehensive analytical tool, we diagnosed a family with severe bleeding problems of unknown origin with Quebec Platelet Diso...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802010547
更新日期:2008-08-01 00:00:00
abstract::Platelet function disorders (PFDs) are a common cause of mild bleeding tendency. However, they cannot be recognized by standard screening studies. The gold standard test for PFD is platelet aggregation, performed by light transmission aggregometry (LTA). A newer and less validated method is the closure time (CT), perf...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1257784
更新日期:2017-11-01 00:00:00
abstract::Selected aspirin treated patients may exhibit high platelet reactivity to agonists other than arachidonic acid. This study aimed to determine whether the VerifyNow identifies generalized high platelet reactivity supported by correlations with other established methods that stimulate platelets with various agonists. St...
journal_title:Platelets
pub_type: 杂志文章,随机对照试验
doi:10.1080/09537100701206824
更新日期:2007-09-01 00:00:00
abstract::Platelet discoid shape is known to correlate with in vivo viability after transfusion. Measurement of shape change requires invasive sampling and laborious assays, which is difficult to perform in a blood transfusion center as a routine test for quality control of stored platelets. The objective of this study was to e...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100600757695
更新日期:2006-09-01 00:00:00
abstract::Platelets are needed to prevent or arrest bleeding and aggregate at the site of injury upon vascular damage. Platelets express receptors for estrogens which might affect the function of the platelets and their hemostatic ability. The aim was to identify possible differences in platelet function related to age, gender,...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2018.1466387
更新日期:2019-01-01 00:00:00
abstract::The SDF-1-CXCR4 axis plays an essential role in the regulation of platelet production, by directing megakaryocyte (MK) migration toward the vascular niche, thus allowing terminal maturation and proplatelet formation, and also regulates platelet function in an autocrine manner. Inherited thrombocytopenias (IT) comprise...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1254763
更新日期:2017-09-01 00:00:00
abstract::Thrombocytopenia is one of the most frequent hematologic abnormalities in the neonatal period, affecting about 18-35% of all patients admitted to the Neonatal Intensive Care Unit (NICU), with sepsis being among the most common causes of severe neonatal thrombocytopenia. It is unclear whether decreased platelet product...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.696748
更新日期:2013-01-01 00:00:00
abstract::The action of inorganic forms of selenium (Se) on blood platelet aggregation, secretion and the arachidonate pathway in vitro was studied. It was shown that sodium selenite, after 30 min preincubation, inhibited platelet aggregation induced by 0.1 U/ml of thrombin and by 10 microM ADP (about 30% inhibition of aggregat...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109976275
更新日期:1999-01-01 00:00:00
abstract::Infection induces platelet activation and consumption, which leads to thrombocytopenia, enhances microvascular thrombosis, impairs microcirculation and eventually triggers disseminated intravascular coagulation (DIC). It is well characterized that endotoxemia results in a pro-inflammatory and pro-coagulatory state, wh...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2015.1119814
更新日期:2016-07-01 00:00:00
abstract::Measurement of platelet aggregation in platelet-rich plasma (PRP) is a fundamental tool in platelet studies, despite the fact that the technique required for this is time-consuming, may need large volumes of blood, and require particular skill and special equipment. The use of a microplate reader seems useful to perfo...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100120071040
更新日期:2001-11-01 00:00:00
abstract::The effects of prostaglandin E(2) (PGE(2)) on platelet function are believed to be the result of opposing mechanisms that lead to both enhancement and inhibition of platelet function. Enhancement of platelet function is known to be via EP3 receptors linked to G(i) and inhibition of adenylyl cyclase. However, the recep...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537101003718065
更新日期:2010-01-01 00:00:00
abstract::Calcium channel blockers are known to inhibit platelet aggregation in vitro and ex vivo. We studied the influence of a single oral dose of diltiazem (60 mg), nifedipine (10 mg) and verapamil (80 mg) on ADP-, collagen-, and epinephrine-induced platelet aggregation, malondialdehyde formation, serum thromboxane B(2) and ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109109005501
更新日期:1991-01-01 00:00:00
abstract::The risk and benefit of GP-IIb/IIIa Inhibition (GPI) in combination with recent antiplatelet regimens in acute coronary syndromes (ACS) remain unassessed. The advent of fast-acting highly active oral P2Y12 inhibitors questions the additional value and risk of their association with GPI. We studied the effect of GPI in...
journal_title:Platelets
pub_type: 杂志文章,meta分析,评审
doi:10.1080/09537104.2016.1218453
更新日期:2017-03-01 00:00:00
abstract::The potential roles of serum butyrylcholinesterase (BChE) activity and platelet indices in type 1 diabetes (T1D) remain uncertain. We aimed to investigate the correlation among the platelet indices, serum BChE activity, and diabetic ketoacidosis (DKA). Sixty-one T1D patients, 29 patients with DKA, and 30 age- and sex-...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.735720
更新日期:2013-01-01 00:00:00
abstract::Despite the transient hyporeactivity of neonatal platelets, full-term neonates do not display a bleeding tendency, suggesting potential compensatory mechanisms which allow for balanced and efficient neonatal hemostasis. This study aimed to utilize small-volume, whole blood platelet functional assays to assess the neon...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2019.1573314
更新日期:2020-01-01 00:00:00
abstract::The purpose of the study was to evaluate safety, effects on platelet aggregation and pharmacokinetics of F(ab')(2) fragments of anti-glycoprotein (GP) IIb-IIIa murine monoclonal antibody FRaMon (F(ab')(2) FRaMon) upon its intravenous administration in patients undergoing high-risk coronary angioplasty. Patients were t...
journal_title:Platelets
pub_type: 临床试验,杂志文章
doi:10.1080/0953710021000057839
更新日期:2002-12-01 00:00:00
abstract::In this study, we have examined whether the platelet Fc-receptor, FcγRII (CD32), is associated with either of the two major platelet membrane glycoproteins, the GPIb-IX complex and the GPIIb-IIIa complex. Monoclonal and polyclonal anti-GPIb-IX complex antibodies inhibited to only a moderate degree (< 40%) the binding ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109309013216
更新日期:1993-01-01 00:00:00
abstract::A number of hematopoietic growth factors and other cytokines are capable of altering platelet production and function. Enhancement of these processes may be exploited to ameliorate bleeding propensity in thrombocytopenic patients. Under certain circumstances, cytokines may have adverse effects on the hemostatic system...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109709169324
更新日期:1997-01-01 00:00:00
abstract::Whether the simultaneous administration of ciprofloxacin or tazobactam/piperacillin increases the risk of thrombocytosis is unknown. Broncho-pulmonary infection in a 50-year-old male with acute, hypertensive, intracerebral bleeding, necessitated therapy with cefpirome (2 g/day, 6 days), ciprofloxacin (800 mg/d, 11 day...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100310001594543
更新日期:2003-08-01 00:00:00
abstract::R-lipoic acid (ALA), a powerful antioxidant valuable for the treatment of diabetes and its complications, has been reported to exhibit an antiplatelet activity in vitro. The aim of this study was to investigate the effect and mechanism of ALA on platelets in vivo. Sprague-Dawley (SD) male rats were intravenously admin...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1356450
更新日期:2018-11-01 00:00:00
abstract::We measured and compared the levels of microparticles, chemokines, cell adhesion molecules and platelet activation markers with a view to developing a better understanding of their potential contributions to the pathophysiology of progressive systemic sclerosis (PSS, scleroderma). The concentrations of all the factors...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100701882038
更新日期:2008-05-01 00:00:00
abstract::Current guidelines favor dual anti-platelet therapy with ticagrelor 90 mg BID (T90BID) over clopidogrel 75 mg QD (C75QD) in addition to aspirin for acute coronary syndrome. However, an increased risk of ticagrelor-related adverse events prompted the evaluation of low-dose regimens. This study (NCT03381742) retrospecti...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1732328
更新日期:2021-01-02 00:00:00
abstract::Ethylenediaminetetraacetic acid (EDTA) causes structural, biochemical and functional damage to blood platelets. The alterations induced are considered irreversible. However, the degree of irreversibility, and whether all functions are similarly compromised by EDTA have not been fully evaluated. The present study has e...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109975979
更新日期:1999-01-01 00:00:00