Abstract:
:Extracellular vesicles (EV) act as a cellular communication tool by carrying lipids, proteins and micro RNA (miR) between cells, thereby playing a pivotal role in thromboembolic processes. The effect of P2Y12 inhibitors on pro-coagulatory, phosphatidylserine (PS)-expressing EV has been investigated previously, but only in vitro or during confounding clinical conditions, such as acute coronary syndrome. Hence, we enrolled 62 consecutive patients 12 month after percutaneous coronary intervention and stent implantation and consequent treatment with dual-antiplatelet therapy consisting of low-dose aspirin and P2Y12 inhibitors. Blood for platelet function testing and EV and miR measurements was taken on the last day of P2Y12 inhibitor intake (baseline, on-treatment) and 10, 30 and 180 days thereafter (off-treatment). We did not observe any influence of P2Y12 inhibitors on the levels of PS-EV or EV sub-population from platelets, erythrocytes, monocytes or endothelial cells, respectively. There was no relationship between platelet function and EV levels in plasma. However, the association of miR-21 and miR-150 with platelet EVs was significantly different between on- and off-treatment measurements. Hence, our study suggests no influence of P2Y12 inhibition on the count of EVs in plasma, but on the potential cargo of platelet-derived EV.
journal_name
Plateletsjournal_title
Plateletsauthors
Haller PM,Stojkovic S,Piackova E,Andric T,Wisgrill L,Spittler A,Wojta J,Huber K,Jäger Bdoi
10.1080/09537104.2019.1648780subject
Has Abstractpub_date
2020-05-18 00:00:00pages
497-504issue
4eissn
0953-7104issn
1369-1635journal_volume
31pub_type
杂志文章相关文献
PLATELETS文献大全abstract::Glycoprotein (GP) IIb-IIIa (αIIbβ3-integrin) is the central receptor of platelet aggregation. Activated GP IIb-IIIa binds fibrinogen or von Willebrand factor, which forms molecular bridges between aggregating platelets. This review summarizes data on the relationship between GP IIb-IIIa expression on the platelet surf...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.3109/09537104.2010.547959
更新日期:2011-01-01 00:00:00
abstract::We prospectively investigated the prevalence of Helicobacter pylori (H. pylori) infection in a cohort of 54 adult Serbian patients with chronic idiopathic thrombocytopenic purpura (ITP), and examined the effects of its eradication on their platelet counts. H. pylori infection was diagnosed in 39/54 (72.2%) patients, u...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500462487
更新日期:2006-06-01 00:00:00
abstract::Combined inhibition of platelet aggregation is essential to prevent recurrent ischemic episodes in patients with acute coronary syndromes and after percutaneous coronary interventions (PCIs). In combination with aspirin, the ADP receptor antagonist clopidogrel is used widespread for this purpose; however, platelet rea...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.3109/09537104.2011.610475
更新日期:2012-01-01 00:00:00
abstract::Bioactive substances found in numerous foods can be successfully and safely used to modify various cellular functions and affect the oxidative stress. Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances shown to have anti-inflammatory, antitumor, antioxidative and antiplate...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537101003612821
更新日期:2010-01-01 00:00:00
abstract::On activation platelets release microRNAs and extracellular vesicles (EV) into circulation. The release of EV from platelets has been shown to be dependent on the agonist; in this study, we investigated whether the microRNA profile or EV released from platelets was also agonist specific. Washed platelets from healthy ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1332366
更新日期:2018-07-01 00:00:00
abstract::Thrombocytopenia is a clinically relevant outcome in HIV. However, the epidemiology of this condition, including frequency, severity, and duration, has not been well-characterized in the era of highly active antiretroviral therapy (HAART). In this study, we describe the epidemiology of thrombocytopenia using two metho...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.582526
更新日期:2011-01-01 00:00:00
abstract::Cytochrome P450 (CYP) 2C19 genotype is closely associated with the metabolism and efficacy of clopidogrel, thereby having an important impact on clinical outcomes of patients with acute coronary syndrome (ACS) or undergoing percutaneous coronary intervention (PCI). This study aimed to evaluate the efficacy and safety ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1780205
更新日期:2020-11-16 00:00:00
abstract::Previous investigations from our laboratory identified the ultrastructural pathology and cytochemistry of macrothrombocytes (MTC) from patients with the X-linked, G208S varient of the GATA-1 mutation.A subsequent biochemical study of the MTC cytoskeletal proteins using polyacrylamide gel electrophoresis and western bl...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100902818344
更新日期:2009-05-01 00:00:00
abstract::Despite the transient hyporeactivity of neonatal platelets, full-term neonates do not display a bleeding tendency, suggesting potential compensatory mechanisms which allow for balanced and efficient neonatal hemostasis. This study aimed to utilize small-volume, whole blood platelet functional assays to assess the neon...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2019.1573314
更新日期:2020-01-01 00:00:00
abstract::Several lines of evidence are consistent with the hypothesis that activated platelets contribute to colorectal tumorigenesis and metastatization through direct cell-cell interactions and the release of different lipid and protein mediators, and microvesicles. This review examines the clinical pharmacology of low-dose ...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2018.1492105
更新日期:2018-12-01 00:00:00
abstract::It has recently been demonstrated that both initiators and inhibitors (viz iloprost) of aggregation stimulate the uptake of [(45)Ca(2+)] by human platelets. Since it was postulated that this calcium uptake reflects changes associated with signal transduction, we investigated the role of cAMP-dependent protein kinases ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109309013234
更新日期:1993-01-01 00:00:00
abstract::Current guidelines favor dual anti-platelet therapy with ticagrelor 90 mg BID (T90BID) over clopidogrel 75 mg QD (C75QD) in addition to aspirin for acute coronary syndrome. However, an increased risk of ticagrelor-related adverse events prompted the evaluation of low-dose regimens. This study (NCT03381742) retrospecti...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1732328
更新日期:2021-01-02 00:00:00
abstract::Plasma lipid composition, platelet aggregation, cholesterol (Ch)/glycoprotein IIb-IIIa (GP) and phospholipid (Ph)/GP molar ratios, fatty acid composition and structural order (1, 6-diphenyl-1, 3, 5-hexatriene (DPH) fluorescence anisotropy at 35 degrees C (r(DPH,35)) of human platelet plasma membranes (HPPM) were measu...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109876438
更新日期:1998-01-01 00:00:00
abstract::Flow cytometric analysis of human platelets from healthy volunteers by high-resolution immunophenotyping accomplished with enzymatic amplification staining revealed the expression of CD34. Assessment of the platelets with five different monoclonal antibodies to the three different classes of CD34 epitopes suggested th...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953710031000080577
更新日期:2003-03-01 00:00:00
abstract::Recent findings have identified critical roles for the actin filament-crosslinking protein filamin A (FlnA) in platelets and megakaryocytes. This short review focuses on the structure of FlnA and its interaction with the Von Willebrand Factor receptor GPIb-IX-V complex and the fibrinogen receptor, the integrin αIIbβ3 ...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.3109/09537104.2011.654004
更新日期:2013-01-01 00:00:00
abstract::The ram gene was isolated from a rat megakaryocyte cDNA library and encodes a GTP-binding protein with a Mr of 25 000. In order to clarify the presence of the ram protein (ram p25) in human platelets, we tried to purify ram p25 from the sodium cholate extract of human platelet membranes by a combination of DEAE-Sephac...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109309013227
更新日期:1993-01-01 00:00:00
abstract::Adenosine diphosphate (ADP) is recognized as an important mediator of platelet aggregation. Transient aggregation at low (< or =1 microM), and sustained aggregation at higher ADP concentrations are consistently observed. Dissociation of platelet aggregates has been described and may explain the reversible component of...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100120071004
更新日期:2001-08-01 00:00:00
abstract::Recent studies have indicated that medical options without splenectomy, such as rituximab (RTX) or thrombopoietin receptor agonists (TPO-RAs), can be effective to treat persistent or chronic primary immune thrombocytopenia (ITP). However, it remains to be determined which of these strategies should be the first choice...
journal_title:Platelets
pub_type: 杂志文章,meta分析
doi:10.1080/09537104.2018.1543864
更新日期:2019-01-01 00:00:00
abstract::High on-treatment platelet reactivity (HTPR) is associated with poor prognosis in patients undergoing percutaneous coronary intervention (PCI). The antiplatelet effect and safety of prasugrel was compared to that of double-dose clopidogrel in patients with stable coronary artery disease or acute coronary syndrome (ACS...
journal_title:Platelets
pub_type: 杂志文章,随机对照试验
doi:10.3109/09537104.2013.845874
更新日期:2014-01-01 00:00:00
abstract::Vascular diseases such as myocardial infarction and ischemic stroke are associated with increased platelet function whilst the risk of recurrence is reduced by antiplatelet agents such as aspirin, clopidogrel, and prasugrel. However, some patients exhibit high platelet reactivity, especially with clopidogrel. Existing...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2018.1445839
更新日期:2018-07-01 00:00:00
abstract::The action of inorganic forms of selenium (Se) on blood platelet aggregation, secretion and the arachidonate pathway in vitro was studied. It was shown that sodium selenite, after 30 min preincubation, inhibited platelet aggregation induced by 0.1 U/ml of thrombin and by 10 microM ADP (about 30% inhibition of aggregat...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109976275
更新日期:1999-01-01 00:00:00
abstract::We present here a 63-year old woman with a long history of immune thrombocytopenia. She was hospitalized for a traumatic intracranial hemorrhage with thrombocytopenia. Following inefficient treatment of four platelet transfusions, immunoglobulins, and corticosteroids, we initiated treatment with a thrombopoietin (TPO)...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1246717
更新日期:2017-07-01 00:00:00
abstract::Severe thrombocytopenia is common in dengue virus (DENV) infections. However, studies focusing on the role of profound thrombocytopenia (PT) (nadir platelet counts ≤ 20 000/mm3) in DENV infections are scarce. This study aims to identify the associated features and outcome of DENV patients with PT. It involves 237 adul...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1293802
更新日期:2017-11-01 00:00:00
abstract::Aspirin is traditionally taken once daily in the morning and considered to be effective throughout the 24h interval. Cardiovascular events occur most frequently in the early morning, suggesting that these hours are critical in terms of adequate platelet inhibition. This study therefore assed platelet function in the e...
journal_title:Platelets
pub_type: 杂志文章,随机对照试验
doi:10.1080/09537104.2018.1528347
更新日期:2019-01-01 00:00:00
abstract::SIRT1, a class III histone deacetylase, is critically involved in cellular response to stress and modulates cardiovascular risk factors. However, its role in thrombus formation is largely unknown. Thus, this study investigated the effect of SIRT1 on pulmonary thrombus formation, and then identified its role in the mod...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1190005
更新日期:2016-12-01 00:00:00
abstract::Cell-cell and cell-extracellular matrix interactions are mediated by a number of membrane glycoproteins. On the basis of structural homologies, several families of cell adhesion molecules (integrins, selectins, immunoglobulins, cadherins, leucine-rich glycoproteins) have been established. Since 1991, a new family of C...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109609023570
更新日期:1996-01-01 00:00:00
abstract::Erythropoietin (EPO) enhances formation of red blood cells and also affects thrombopoiesis and platelet function. We hypothesized that the effect of EPO may be reflected by changes in thromboxane B2 (TXB2) and endothelial cell function. Six male and six female subjects received recombinant human epoetin alpha (Erypo®)...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.631621
更新日期:2012-01-01 00:00:00
abstract::Oxidative damage to lipids in plasma, blood platelets and neurons in patients with schizophrenia was described. The aim of our present study was to evaluate oxidative/nitrative modifications of blood platelets proteins by measurement the level of biomarkers of oxidative stress such as carbonyl groups, thiol groups and...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802641499
更新日期:2009-03-01 00:00:00
abstract::Prostaglandins stimulate cyclic AMP synthesis in platelets, leading to inhibition of platelet aggregation. Prostaglandin receptors have been identified but knowledge of their pharmacology is limited by the lack of antagonists. Kinetic measurements of prostaglandinregulated cyclic AMP formation provide useful informati...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109009009190
更新日期:1990-01-01 00:00:00
abstract::A platelet substitute, Synthocytes, is being developed for the prevention and treatment of thrombocytopenia. Synthocytes are composed of fibrinogen adsorbed on heat stabilised albumin microcapsules of defined size. The purpose of this study was to perform experiments in vitro to investigate the capacity of Synthocytes...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953371027000
更新日期:2002-06-01 00:00:00