Abstract:
:The ram gene was isolated from a rat megakaryocyte cDNA library and encodes a GTP-binding protein with a Mr of 25 000. In order to clarify the presence of the ram protein (ram p25) in human platelets, we tried to purify ram p25 from the sodium cholate extract of human platelet membranes by a combination of DEAE-Sephacel, Sephacryl S300HR, hydroxyapatite HCA-100S and DEAE-Toyopearl 650(S) column chromatographies. In the course of the purification, a specific antibody raised against a synthetic COOH-terminal peptide of ram p25 was used. In conclusion, ram p25 was partially purified and was observed to be present in membrane fractions of human platelets.
journal_name
Plateletsjournal_title
Plateletsauthors
Nagata K,Okano Y,Suzuki T,Nozawa Ydoi
10.3109/09537109309013227subject
Has Abstractpub_date
1993-01-01 00:00:00pages
268-74issue
5eissn
0953-7104issn
1369-1635journal_volume
4pub_type
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