Abstract:
:Plasma lipid composition, platelet aggregation, cholesterol (Ch)/glycoprotein IIb-IIIa (GP) and phospholipid (Ph)/GP molar ratios, fatty acid composition and structural order (1, 6-diphenyl-1, 3, 5-hexatriene (DPH) fluorescence anisotropy at 35 degrees C (r(DPH,35)) of human platelet plasma membranes (HPPM) were measured in four DPH,35 groups of hyperlipidemic patients (II: plasma Ch < 250 mg/dl and TG (triglycerides) <220 mg/dl, n = 21; III: Ch > 250 mg/dl and TG < 220 mg/dl, n = 23; IV: Ch < 250 mg/dl and TG > 220 mg/dl, n = 18; and V: Ch > 250 mg/dl and TG > 220 mg/dl, n = 12) and compared with those of the control group (I). Our results were: (i) in groups III, IV and V the HPPM (Ch + Ph)/GP molar ratio increased 7.0+/-7.7% (mean SD); (ii) the Ph/GP molar ratio increased significantly in groups III, IV and V, but most in IV and V, while the Ch/GP molar ratio increased only in groups III and V; (iii) the mean relative increase of Ch with respect to Ph in the HPPM of groups III, IV and V was 140% 21% and 54%, respectively; (iv) the Ch/GP molar ratio was correlated with LDL-Ch (0.41+/-0.16, P < 0.002, n = 55, for all the subjects and 0.60+/-0.11, P < 2.10(-4), n = 33, for subjects with TG < 220 mg/dl), however, it was totally uncorrelated with HDL-Ch; (v) the HPPM Ch/Ph molar ratio was positively correlated with plasma Ch (r = 0.51+/-0.08, P < 1.10(-6), n = 83) and with (LDL + HDL) Ch (r = 0.64+/-0.07, P < 1.10(-6), n = 73), the former correlation increased significantly ( r = 0.67+/-0.07, P < 1.10(-6), n = 53) when done only for subjects with TG < 220 mg/dl; (vi) the Ch/Ph molar ratio was only increased in group III (0.70+/-0.03, P < 3.10(-5), n = 23) and decreased in group IV (0.62+/-0.02, P < 0.001, n = 18); (vii) the fatty acid/GP molar ratio was significantly increased in groups IV and V, however, a significant absolute and relative increase of C16:0 and C18:1 was observed only in severe hypertriglyceridemia (> 500 mg/dl), together with a relative decrease of C18:0 and C20:4 ( n - 6); (viii) the HPPM structural order, as probed by r(DPH,35), was negatively correlated with DPH,35 plasma TG (r =- 0.61+/-0.10, P < 4.10(-5), n = 39), the Ph/GP molar ratio (r =-0.58+/-0.10, P < 2.10(-4), n = 39) and the the (C18:1 + C18:2))/GP molar ratio (r =- 0.80+/-0.05, P < 1.10(-6), n = 39), however, it was independent of plasma and HPPM Ch; (ix) the higher HPPM Ch/Ph molar ratio in group III was associated (r = 0.58+/-0.12, P < 0.005, n = 22) with a moderately higher platelet reactivity to collagen. We conclude that Ch and Ph were distinctly incorporated to HPPM in the different groups of hyperlipidemia and, therefore, that the absolute increase of Ch and Ph was more informative to understand the structural and functional modifications of the HPPM in hyperlipidemias, than the Ch/Ph molar ratio. On the other hand, the r was sensitive to the DPH,35 increase in the content of HPPM Ph and C18:1 + C18:2 and it was insensitive to the increase in the Ch content.
journal_name
Plateletsjournal_title
Plateletsauthors
García-Guerra R,Mateo CR,García-Muñoz MS,Zapata MA,Plaza I,González-Rodríguez Jdoi
10.1080/09537109876438subject
Has Abstractpub_date
1998-01-01 00:00:00pages
365-73issue
6eissn
0953-7104issn
1369-1635pii
L1E2LPEEQK95RRLTjournal_volume
9pub_type
杂志文章相关文献
PLATELETS文献大全abstract::A platelet substitute, Synthocytes, is being developed for the prevention and treatment of thrombocytopenia. Synthocytes are composed of fibrinogen adsorbed on heat stabilised albumin microcapsules of defined size. The purpose of this study was to perform experiments in vitro to investigate the capacity of Synthocytes...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953371027000
更新日期:2002-06-01 00:00:00
abstract::Human melanoma cell Line, HMV-I, has been shown to induce platelet aggregation without thrombin generation. The platelet-aggregating activity of HMV-I cells increased with increasing culture periods of the cells. However, data indicate that the platelet-aggregating activity of HMV-I cells is dependent upon the cell de...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509078456
更新日期:1995-01-01 00:00:00
abstract::This manuscript reviews pathogenic variants in RASGRP2, which are the cause of a relatively new autosomal recessive and nonsyndromic inherited platelet function disorder, referred to as platelet-type bleeding disorder-18 (BDPLT18)(OMIM:615888). To date, 18 unrelated BDPLT18 pedigrees have been reported, harboring 19 d...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2019.1585528
更新日期:2019-01-01 00:00:00
abstract::To study the possibility of using thrombin generation tests in platelet-rich and platelet-poor plasma for evaluation of dual antiplatelet therapy efficacy in patients with coronary artery disease (CAD), following percutaneous coronary intervention. Venous blood was analyzed from CAD patients aged 53-75 years who had u...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1294680
更新日期:2018-03-01 00:00:00
abstract::The tetraspanins are a superfamily of membrane glycoproteins which facilitate the interaction of membrane and intracellular signalling molecules (e.g., integrins, pro-growth factors and their receptors, protein kinase-C) in the formation of membrane signalling microdomains (sometimes referred to as the tetraspanin web...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537100400004363
更新日期:2005-02-01 00:00:00
abstract::Information regarding the profile of reticulated platelets (RP) in ischemic cerebrovascular disease (CVD) patients is limited. Data from two prospective, observational, case-control studies were combined to compare the %RP using whole blood flow cytometry in patients ≤ 4 weeks of TIA/stroke onset (baseline, N = 210), ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1850670
更新日期:2020-12-21 00:00:00
abstract::Oxidative damage to lipids in plasma, blood platelets and neurons in patients with schizophrenia was described. The aim of our present study was to evaluate oxidative/nitrative modifications of blood platelets proteins by measurement the level of biomarkers of oxidative stress such as carbonyl groups, thiol groups and...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802641499
更新日期:2009-03-01 00:00:00
abstract::Recent findings have identified critical roles for the actin filament-crosslinking protein filamin A (FlnA) in platelets and megakaryocytes. This short review focuses on the structure of FlnA and its interaction with the Von Willebrand Factor receptor GPIb-IX-V complex and the fibrinogen receptor, the integrin αIIbβ3 ...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.3109/09537104.2011.654004
更新日期:2013-01-01 00:00:00
abstract::In adults with immune thrombocytopenic purpura (ITP), steroids are usually proposed as first-line therapy, but long-term complete responses are obtained in no more than 20% of patients. For the remaining patients, splenectomy is considered the treatment of choice, with reported "cure" rates from 60-70%. However, the i...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802315110
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:Thrombocytopenia has been implicated in patients infected with severe acute respiratory syndrome coronavirus 2, while the association of platelet count and changes with subsequent mortality remains unclear. METHODS:The clinical and laboratory data of 383 patients with the definite outcome by March 1, 2020 i...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1754383
更新日期:2020-05-18 00:00:00
abstract::Cell-cell and cell-extracellular matrix interactions are mediated by a number of membrane glycoproteins. On the basis of structural homologies, several families of cell adhesion molecules (integrins, selectins, immunoglobulins, cadherins, leucine-rich glycoproteins) have been established. Since 1991, a new family of C...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109609023570
更新日期:1996-01-01 00:00:00
abstract::The H(1)-histamine receptor antagonist Dithaden(R) inhibited in a dose-dependent manner, human platelet aggregation in vitro that was induced with stimuli in the following rank order of potency: thrombin > A23187 > adrenaline > ADP. The aggregation of platelets in plasma induced with adrenaline was inhibited by Dithia...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109709169334
更新日期:1997-01-01 00:00:00
abstract::Although (very) late coronary stent thrombosis is a rare complication after percutaneous coronary interventions (PCI), its consequences are devastating with a high morbidity and mortality. Previous studies have identified several clinical, procedural and angiographic characteristics that are associated with stent thro...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537100903388367
更新日期:2010-01-01 00:00:00
abstract::Most agonists stimulate platelet Ca2+ rises via G-protein coupled receptors (GPCRs) or ITAM-linked receptors (ILRs). Well studied are the GPCRs stimulated by the soluble agonists thrombin (PAR1, PAR4), ADP (P2Y1, P2Y12), and thromboxane A2 (TP), signaling via phospholipase (PLC)β isoforms. The platelet ILRs glycoprote...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1859103
更新日期:2020-12-26 00:00:00
abstract::One hundred and sixty-eight children aged 13 months to 12.6 years with acquired platelet dysfunction with eosinophilia (APDE) were studied. The male to female ratio was 1.15:1. All of the children were in good health and no history of any drug ingestion was detected. All of the children had widespread spontaneous brui...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100020031180
更新日期:2001-02-01 00:00:00
abstract::Platelets are involved in host defense via clearance of bacteria from the circulation, interaction with virus particles, and uptake of various size particulates. There is a growing interest in micro- and nanoparticles for drug delivery and there is evidence that the properties of these particles critically influence t...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.703792
更新日期:2013-01-01 00:00:00
abstract::Platelets play a key role in thrombotic vascular occlusion at the side of an atherosclerotic plaque. Malondialdehyde (MDA) derivatives are components of oxidized LDL. Recent studies suggest enhanced adhesion of platelets on oxidized LDL, but evidence of MDA-modified LDL contribution to enhanced platelet adhesion is mi...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500261590
更新日期:2006-03-01 00:00:00
abstract::Dual antiplatelet therapy (DAPT) with aspirin and a P2Y12 inhibitor is a mainstay of the prevention of stent thrombosis following percutaneous coronary intervention (PCI). In the 2015 European guidelines for the management of acute coronary syndrome (ACS), prasugrel (PRA) and ticagrelor (TICA) combined with aspirin ar...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2018.1445836
更新日期:2019-01-01 00:00:00
abstract::Standardized Ginkgo biloba extract, EGb761, has been shown to possess polyvalent properties, such as anti-oxidation, anti-apoptosis and anti-inflammation. Recently, it has also been proposed to have direct protective effects on mitochondria. The effects of EGb761 make it a potential anti-aging drug. Despite that, the ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537100903511448
更新日期:2010-01-01 00:00:00
abstract::The potential roles of serum butyrylcholinesterase (BChE) activity and platelet indices in type 1 diabetes (T1D) remain uncertain. We aimed to investigate the correlation among the platelet indices, serum BChE activity, and diabetic ketoacidosis (DKA). Sixty-one T1D patients, 29 patients with DKA, and 30 age- and sex-...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.735720
更新日期:2013-01-01 00:00:00
abstract::Platelet function disorders (PFDs) are a common cause of mild bleeding tendency. However, they cannot be recognized by standard screening studies. The gold standard test for PFD is platelet aggregation, performed by light transmission aggregometry (LTA). A newer and less validated method is the closure time (CT), perf...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1257784
更新日期:2017-11-01 00:00:00
abstract::In the presence of large bone defects, delayed bone union, or nonunion and fractures, bone reconstruction may be necessary. Different strategies have been employed to enhance bone healing among which the use of autologous platelet concentrates (APCs). Due to the high content of platelets and platelet-derived bioactive...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2017.1384537
更新日期:2018-06-01 00:00:00
abstract::Tissue factor (TF) is the most important initiator of intravascular coagulation. This article will review published evidence on the contribution of platelets to TF exposure to the circulating blood. The following mechanisms will be discussed: decryption of monocyte TF by platelets, contribution of platelets to TF expr...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537100500140190
更新日期:2005-09-01 00:00:00
abstract::No consensus exists regarding the optimal estimate of light transmission aggregometry (LTA) to reflect P2Y12 ADP receptor inhibition in patients receiving thienopyridine therapy. Currently, the only completely P2Y12-receptor specific method is the measurement of vasodilator stimulated phosphoprotein (VASP) phosphoryla...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.494742
更新日期:2010-01-01 00:00:00
abstract::Platelet shape change is an extremely rapid process mediated by both the calcium-sensitive and p160ROCK pathways. The present study examines how different features of shape change studied by scanning electron microscopy clearly correlate to changes in the pattern of light absorbance measured in an aggregometer. Platel...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953710031000092794
更新日期:2003-05-01 00:00:00
abstract::Bioactive substances found in numerous foods can be successfully and safely used to modify various cellular functions and affect the oxidative stress. Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances shown to have anti-inflammatory, antitumor, antioxidative and antiplate...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537101003612821
更新日期:2010-01-01 00:00:00
abstract::Platelet-rich fibrin (PRF) is generated from the patients' own venous blood by a single centrifugation step without the additional use of anticoagulants. Based on the previously described LSCC (low-speed centrifugation concept), our group showed that modification of the centrifugation setting, that is, reducing the re...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2018.1445835
更新日期:2019-01-01 00:00:00
abstract::Peroxynitrite is a potent nitrating and oxidizing agent that exerts differential effects on platelets. In the present study we investigated the influence of peroxynitrite on vasodilator-stimulated phosphoprotein (VASP), a protein that plays a key role in inhibition of platelet adhesion and spreading. In platelets, VAS...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.483296
更新日期:2010-01-01 00:00:00
abstract::The aim of the study was to evaluate diurnal changes of tissue factor (TF) and tissue factor pathway inhibitor (TFPI) concentrations in relation to on-treatment platelet reactivity. The study group included 51 patients with acute myocardial infarction (AMI) treated with primary percutaneous coronary intervention and d...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2019.1693037
更新日期:2020-10-02 00:00:00
abstract::Whether the simultaneous administration of ciprofloxacin or tazobactam/piperacillin increases the risk of thrombocytosis is unknown. Broncho-pulmonary infection in a 50-year-old male with acute, hypertensive, intracerebral bleeding, necessitated therapy with cefpirome (2 g/day, 6 days), ciprofloxacin (800 mg/d, 11 day...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100310001594543
更新日期:2003-08-01 00:00:00