Dynamic regulation of microtubule coils in ADP-induced platelet shape change by p160ROCK (Rho-kinase).

Abstract:

:Platelet shape change is an extremely rapid process mediated by both the calcium-sensitive and p160ROCK pathways. The present study examines how different features of shape change studied by scanning electron microscopy clearly correlate to changes in the pattern of light absorbance measured in an aggregometer. Platelets change shape from the initial 'disc' form by producing: membrane 'blebs', sphere formation (cell-rounding), filopodia extension, and surface membrane folding. The presentation of these features was dramatically slower in the absence of intracellular calcium mobilization. In the presence of the p160ROCK-inhibitor, Y-27632, shape change was initially normal but platelets rapidly transformed back to smooth discs with extended filopodia. The reappearance of the disc shape is reflected by an increase in the amplitude of oscillations in the aggregometer shape change tracing. The kinetics of actin/cytoskeleton association correlated with filopodia formation but not with disc to sphere transformation. Changes in the level of tubulin polymerization correlated with changes from disc to sphere morphology. These experiments are consistent with a role for a RhoA/Rho kinase-regulated pathway in the maintenance of a spherical platelet shape after agonist-dependent activation. Continued disruption of the cytoskeletal microtubule ring, appears to be a Rhokinase-dependent event involved in the transformation of discoid platelets into spheres.

journal_name

Platelets

journal_title

Platelets

authors

Paul BZ,Kim S,Dangelmaier C,Nagaswami C,Jin J,Hartwig JH,Weisel JW,Daniel JL,Kunapuli SP

doi

10.1080/0953710031000092794

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

159-69

issue

3

eissn

0953-7104

issn

1369-1635

pii

0TLLEQ6KYKBDG94T

journal_volume

14

pub_type

杂志文章
  • R-lipoic acid overdosing affects platelet life span via ROS mediated autophagy.

    abstract::R-lipoic acid (ALA), a powerful antioxidant valuable for the treatment of diabetes and its complications, has been reported to exhibit an antiplatelet activity in vitro. The aim of this study was to investigate the effect and mechanism of ALA on platelets in vivo. Sprague-Dawley (SD) male rats were intravenously admin...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2017.1356450

    authors: Liu J,Song Q,Huang Y,Sun W,Lu D,Zhou B

    更新日期:2018-11-01 00:00:00

  • Platelet aggregation in erythropoietin treated dialysis patients.

    abstract::Recombinant human erythropoietin is a successful treatment for the anaemia of renal failure. Its use has however been associated with thromboembolic complications. Changes in platelet number and behaviour in vitro were studied in: (1) 23 haemodialysis patients, and 10 continuous ambulatory peritoneal dialysis patients...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537109209013168

    authors: Taylor JE,Henderson IS,Stewart WK,Belch JJ

    更新日期:1992-01-01 00:00:00

  • A new point-of-care method for monitoring anti-platelet therapy: application of the cone and plate(let) analyzer.

    abstract::Recent studies suggest that anti-platelet agents are not equally effective in all individuals. We have developed a new method to evaluate the effect of anti-platelet drugs using the cone and plate(let) analyzer (CPA) test. The method is based on the ability of activators to reduce platelet adhesion under flow conditio...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537100400028800

    authors: Spectre G,Brill A,Gural A,Shenkman B,Touretsky N,Mosseri E,Savion N,Varon D

    更新日期:2005-08-01 00:00:00

  • Hyperthermia induces platelet apoptosis and glycoprotein Ibalpha ectodomain shedding.

    abstract::Hemorrhage is a significant pathological feature of some fever or hyperthermia-related diseases, such as dengue fever and heatstroke. Although the mechanisms of hemorrhage in these diseases are thought to be complex, whether there is an association between hemorrhage and hyperthermia or fever remains unclear. Platelet...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537100903443949

    authors: Wang Z,Shi Q,Li S,Du J,Liu J,Dai K

    更新日期:2010-01-01 00:00:00

  • GPVI modulation during platelet activation and storage: its expression levels and ectodomain shedding compared to markers of platelet storage lesion.

    abstract::Platelet storage is associated with deleterious changes leading to the loss of platelet reactivity and response. During storage, platelets experience increased expression and shedding of P-selectin and CD40L as specific markers of platelet activation, whereas GPIbα decreases due to ectodomain shedding. As an important...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2016.1235692

    authors: Hosseini E,Ghasemzadeh M,Nassaji F,Jamaat ZP

    更新日期:2017-07-01 00:00:00

  • The role of platelet microvesicles in intercellular communication.

    abstract::In recent years, there has been exponential growth in the interest in microvesicles, which is reflected by the number of publications. Initially referred to as "platelet dust" by Peter Wolf in 1967, platelet microvesicles (PMV) are now recognized as important mediators of intercellular communication. There are example...

    journal_title:Platelets

    pub_type: 杂志文章,评审

    doi:10.1080/09537104.2016.1257114

    authors: Edelstein LC

    更新日期:2017-05-01 00:00:00

  • Human platelets contain and release TWEAK.

    abstract::The multifunctional cytokine, TWEAK (TNF-like weak inducer of apoptosis), is a member of the TNFα superfamily. TWEAK is found in a broad range of cell types and has been linked to cell growth and survival, angiogenesis and other inflammatory processes. These functions and their importance in inflammatory diseases have...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537104.2010.512403

    authors: Meyer T,Amaya M,Desai H,Robles-Carrillo L,Hatfield M,Francis JL,Amirkhosravi A

    更新日期:2010-01-01 00:00:00

  • Activation of Phospholipase C as a Primary Target of the Thromboxane A(2)-mediated Amplification Mechanism in Thrombin-induced Rabbit Platelet Activation.

    abstract::The inhibitory effects of a cyclooxygenase inhibitor, indomethacin, and a thromboxane (TX) A(2) receptor antagonist, S-145, on thrombin-stimulated rabbit platelet responses were examined for their contribution to the TXA-mediated amplification mechanism. Although thrombin (0.01-0.3 U/ml) induced the dosedependent aggr...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537109409006037

    authors: Kawamura M,Huang A,Harada Y,Katori M

    更新日期:1994-01-01 00:00:00

  • Predicting metabolic syndrome by using hematogram models in elderly women.

    abstract:BACKGROUND:Low-grade inflammatory status was thought to be a major underlying mechanism in MetS. White blood cell (WBC) count was one of the inflammatory markers identified to be associated with MetS. Moreover, not only WBC but also hemoglobin (Hb) and platelet (PLT) were all associated with MetS. OBJECTIVE:In this st...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537104.2013.780017

    authors: Liu H,Hsu CH,Lin JD,Hsieh CH,Lian WC,Wu CZ,Pei D,Chen YL

    更新日期:2014-01-01 00:00:00

  • The expression of IFN-gamma, IL-4, Foxp3 and perforin genes are not correlated with DNA methylation status in patients with immune thrombocytopenic purpura.

    abstract::We investigated the relationship between the expression of IFN-gamma, IL-4, Foxp3 and perforin gene and the methylation status of their promoters in immune thrombocytopenic purpura (ITP) patients. Gene expression and DNA methylation were determined by quantitative PCR and bisulfite genomic sequencing, respectively. Th...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537100903420277

    authors: Zhao H,Du W,Wang D,Gu D,Xue F,Ge J,Sui T,Yang R

    更新日期:2010-01-01 00:00:00

  • Thrombocytosis under ciprofloxacin and tazobactam/piperacillin.

    abstract::Whether the simultaneous administration of ciprofloxacin or tazobactam/piperacillin increases the risk of thrombocytosis is unknown. Broncho-pulmonary infection in a 50-year-old male with acute, hypertensive, intracerebral bleeding, necessitated therapy with cefpirome (2 g/day, 6 days), ciprofloxacin (800 mg/d, 11 day...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537100310001594543

    authors: Finsterer J,Kotzailias N

    更新日期:2003-08-01 00:00:00

  • GATA-1, G208S macrothrombocytes are deficient in talin: immunofluorescence studies.

    abstract::Previous investigations from our laboratory identified the ultrastructural pathology and cytochemistry of macrothrombocytes (MTC) from patients with the X-linked, G208S varient of the GATA-1 mutation.A subsequent biochemical study of the MTC cytoskeletal proteins using polyacrylamide gel electrophoresis and western bl...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537100902818344

    authors: White JG,Burris SM,Crowe BR

    更新日期:2009-05-01 00:00:00

  • Vigorous exercise as a triggering mechanism for late stent thrombosis: A description of three cases.

    abstract::Although (very) late coronary stent thrombosis is a rare complication after percutaneous coronary interventions (PCI), its consequences are devastating with a high morbidity and mortality. Previous studies have identified several clinical, procedural and angiographic characteristics that are associated with stent thro...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537100903388367

    authors: Zwart B,Van Kerkvoorde TC,van Werkum JW,Breet NJ,Ten Berg JM,Van 't Hof AW

    更新日期:2010-01-01 00:00:00

  • Diversity of signaling underlying responses mediated by selective activation of the platelet thromboxane A2 (TXA2) receptor; pharmacological and kinetic studies in vitro.

    abstract::Selective activation of the platelet TXA2 receptor is sufficient to mediate concurrent aggregation, deaggregation and shape change (SC) responses without activation of known Gi-coupled receptors (Platelets 2003; 14: 89). However, Gi-coupled receptor activation strongly influences the hemostasis response in vivo. This ...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537100310001612380

    authors: Maayani S,Schwarz TE,Patel ND,Craddock-Royal BD,Tagliente TM

    更新日期:2003-09-01 00:00:00

  • Purinergic receptors on blood platelets.

    abstract::Extracellular adenine nucleotides interact with P2 purinergic receptors to regulate a broad range of physiological processes among which platelet aggregation P2 purinoceptors were divided into two main groups: the G-protein coupled receptor or 'metabotropic' superfamily termed P2Y and the ligand-gated ion channel or '...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537109609023587

    authors: Gachet C,Hechler B,Léon C,Vial C,Ohlmann P,Cazenave JP

    更新日期:1996-01-01 00:00:00

  • Time dependent reduction in platelet aggregation using the multiplate analyser and hirudin blood due to platelet clumping.

    abstract::The Multiplate is a popular instrument that measures platelet function using whole blood. Potentially considered a point of care instrument, it is also used by hemostasis laboratories. The instrument is usually utilized to assess antiplatelet medication or as a screen of platelet function. According to the manufacture...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2017.1384540

    authors: Chapman K,Favaloro EJ

    更新日期:2018-05-01 00:00:00

  • Update on functional and genetic laboratory assays for the detection of platelet microvesicles.

    abstract::Functional and genetic assays for measuring platelet microvesicles (PMVs) are presented and discussed. Functional assays concern two groups of methods: a) homogeneous assays using the cofactor activity of phospholipids (PPLs) contained in PMVs and present in assayed plasmas, and a coagulation or a thrombin generation ...

    journal_title:Platelets

    pub_type: 杂志文章,评审

    doi:10.1080/09537104.2016.1265925

    authors: Laroche M,Dunois C,Vissac AM,Amiral J

    更新日期:2017-05-01 00:00:00

  • Prevalence, severity, and duration of thrombocytopenia among HIV patients in the era of highly active antiretroviral therapy.

    abstract::Thrombocytopenia is a clinically relevant outcome in HIV. However, the epidemiology of this condition, including frequency, severity, and duration, has not been well-characterized in the era of highly active antiretroviral therapy (HAART). In this study, we describe the epidemiology of thrombocytopenia using two metho...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537104.2011.582526

    authors: Vannappagari V,Nkhoma ET,Atashili J,Laurent SS,Zhao H

    更新日期:2011-01-01 00:00:00

  • Mean platelet volume and eosinophilia relationship in patients with bullous pemphigoid.

    abstract:INTRODUCTION:Bullous pemphigoid (BP) is an autoimmune skin disease that is characterized by the formation of blisters on normal or erythematous skin. In patients with BP, eosinophils are highly represented in the inflammatory infiltrate of the lesional skin and their levels are often increased in peripheral blood. It i...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537104.2013.784735

    authors: Rifaioglu EN,Sen BB,Ekiz Ö,Dogramaci AC

    更新日期:2014-01-01 00:00:00

  • Development of a high-yield expression and purification system for platelet factor 4.

    abstract::Heparin-induced thrombocytopenia (HIT) is an adverse drug reaction characterized by IgG antibodies bound to complexes of platelet factor 4 (PF4) and heparin. The majority of diagnostic tests for HIT rely on an exogenous source of PF4 to identify anti-PF4/heparin antibodies. These include the PF4-dependent enhanced ser...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2017.1378808

    authors: Huynh A,Arnold DM,Moore JC,Smith JW,Kelton JG,Nazy I

    更新日期:2018-05-01 00:00:00

  • Comparison of treatments for persistent/chronic immune thrombocytopenia: a systematic review and network meta-analysis.

    abstract::Recent studies have indicated that medical options without splenectomy, such as rituximab (RTX) or thrombopoietin receptor agonists (TPO-RAs), can be effective to treat persistent or chronic primary immune thrombocytopenia (ITP). However, it remains to be determined which of these strategies should be the first choice...

    journal_title:Platelets

    pub_type: 杂志文章,meta分析

    doi:10.1080/09537104.2018.1543864

    authors: Arai Y,Matsui H,Jo T,Kondo T,Takaori-Kondo A

    更新日期:2019-01-01 00:00:00

  • High plasma levels of adipocytokines are associated with platelet activation in patients with coronary artery disease.

    abstract::Previous studies suggested that the adipocytokines, adiponectin and leptin, are associated with the progression and prognosis of coronary artery disease (CAD). The aim of this study was to differentially evaluate plasma levels of adiponectin and leptin in patients with CAD and their association with conventional labor...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537100903377584

    authors: Bigalke B,Stellos K,Geisler T,Seizer P,Mozes V,Gawaz M

    更新日期:2010-01-01 00:00:00

  • A comparative study of antioxidative activity of calcium-D-glucarate, sodium-D-gluconate and D-glucono-1,4-lactone in a human blood platelet model.

    abstract::D-glucono-1,4-lactone, sodium D-gluconate and calcium D-glucarate are non-toxic glucose derivatives occurring naturally in fruits and vegetables. Calcium D-glucarate is promoted as an orally bioavailability dietary supplement with potential chemopreventive activity without adverse effects. Despite many commercial appl...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537104.2010.512210

    authors: Saluk-Juszczak J

    更新日期:2010-01-01 00:00:00

  • Impaired responsiveness to clopidogrel and aspirin in patients with recurrent stent thrombosis following percutaneous intervention for peripheral artery disease.

    abstract::Patients with peripheral artery disease (PAD) following peripheral percutaneous transluminal angioplasty (PTA) with stent implantation are prone to stent thrombosis despite treatment with aspirin and clopidogrel. Impaired clopidogrel responsiveness is associated with increased risk of ischemic events in patients follo...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.3109/09537104.2012.676220

    authors: Mazur P,Frołow M,Niżankowski R,Sadowski J,Undas A

    更新日期:2013-01-01 00:00:00

  • Efficacy and safety of different ticagrelor regimens versus clopidogrel in patients with coronary artery disease: a retrospective multicenter study (SUPERIOR).

    abstract::Current guidelines favor dual anti-platelet therapy with ticagrelor 90 mg BID (T90BID) over clopidogrel 75 mg QD (C75QD) in addition to aspirin for acute coronary syndrome. However, an increased risk of ticagrelor-related adverse events prompted the evaluation of low-dose regimens. This study (NCT03381742) retrospecti...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2020.1732328

    authors: Shi J,He M,Wang W,Liu G,Zhang H,Sun D,Li J,Mu H,Xu M,Zhao C,Wang J,Zhang C,Cang H,Zhao S,Zhang Z,Li Y

    更新日期:2021-01-02 00:00:00

  • Frozen extracellular matrix preserves its thrombogenicity after thawing, while Matrigel induces a poor platelet response.

    abstract::Two putative substitutes for fresh endothelial cell (EC) extracellular matrix (ECM), frozen ECM and Matrigel, were studied using a parallel-plate perfusion system and platelet deposition was evaluated morphometrically. Coverslips covered with ECM were stored frozen at-30 C for 0 (fresh ECM), 1, 2, 3 or 4 weeks. The ab...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537109876500

    authors: Bozzo J,Gómez-Ortiz G,Hernández MR,Garrido M,Ordinas A,Escolar G,Díaz-Ricart M

    更新日期:1998-01-01 00:00:00

  • Aging of platelets stored for transfusion.

    abstract::A goal of platelet storage is to maintain the quality of platelets from the point of donation to the point of transfusion - to suspend the aging process. This effort is judged by clinical and laboratory measures with varying degrees of success. Recent work gives encouragement that platelets can be maintained ex vivo b...

    journal_title:Platelets

    pub_type: 杂志文章,评审

    doi:10.3109/09537104.2016.1171303

    authors: Smethurst PA

    更新日期:2016-09-01 00:00:00

  • Thrombin generation test for evaluation of antiplatelet treatment in patients with coronary artery disease after percutaneous coronary intervention.

    abstract::To study the possibility of using thrombin generation tests in platelet-rich and platelet-poor plasma for evaluation of dual antiplatelet therapy efficacy in patients with coronary artery disease (CAD), following percutaneous coronary intervention. Venous blood was analyzed from CAD patients aged 53-75 years who had u...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2017.1294680

    authors: Berezovskaya G,Smirnova O,Malev E,Khromov-Borisov N,Klokova E,Karpenko M,Papayan L,Petrishchev N

    更新日期:2018-03-01 00:00:00

  • Two pathways for store-mediated calcium entry in human platelets.

    abstract::In human platelets and other non-excitable cell types depletion of the intracellular calcium stores promotes calcium entry across the plasma membrane. Although the mechanism of this store-mediated calcium entry remains uncertain, it has been suggested that a tyrosine phosphorylation step could be involved. In support ...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537100050057657

    authors: Jenner S,Sage SO

    更新日期:2000-06-01 00:00:00

  • Expression of ADP receptor P2Y12, thromboxane A2 receptor and C-type lectin-like receptor 2 in cord blood-derived megakaryopoiesis.

    abstract::The ADP receptor P2Y12, the thromboxane A2 receptor (TXA2R) and the C-type lectin-like receptor 2 (CLEC-2) mediate platelet activation by different mechanisms. Only little is known about the expression of the receptors in human megakaryopoiesis. Our study aimed to establish a flow cytometry (FC) method for the measure...

    journal_title:Platelets

    pub_type: 杂志文章

    doi:10.1080/09537104.2020.1782868

    authors: Gerhards C,Uhlig S,Etemad M,Christodoulou F,Bieback K,Klüter H,Bugert P

    更新日期:2020-07-03 00:00:00