Abstract：
:The need for a rapid and sensitive microbiological assay has become necessary for both research and clinical diagnostic purposes. This need has become clear as a result of extensive documentation linking specific bacterial species and periodontal destruction. DNA probe technology provides both a sensitive and specific assay and alleviates the concern for transport of fastidious microorganisms. The DNA probe procedure includes (1) disruption of bacterial cells with denaturation of DNA, (2) immobilization of DNA onto a nitrocellulose filter, (3) blocking unbound nitrocellulose with non-specific DNA, (4) hybridization of the filter with 32P-labelled probe, (5) washing and detection of bound probe. At our laboratory, microbiological analysis with whole-genomic and cloned DNA probes has been used on thousands of plaque specimens in several large-scale research projects. In one study, levels (greater than or equal to 10(5)) of Actinobacillus actinomycetemcomitans were significantly higher in subjects under 21 yr than in subjects with adult periodontitis (over 21 yr old). In another study, the distribution of periodontal pathogens throughout the mouth was examined. High levels were selectively found at sites with probing depths of 5 mm or more and bleeding on probing, directing specimen collection to these sites. In contrast, random selection of sites for sampling was found to be a poor method for detecting high levels of pathogens. These data suggest appropriate sites for specimen collection for further research and diagnostic purposes.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Savitt ED,Keville MW,Peros WJdoi
10.1016/0003-9969(90)90147-3subject
Has Abstractpub_date
1990-01-01 00:00:00pages
153S-159Seissn
0003-9969issn
1879-1506journal_volume
35 Supplpub_type
杂志文章abstract：UNLABELLED:Tooth enamel is formed by ameloblasts, which are derived from the epithelial cells of the enamel organ. OBJECTIVE:The purpose of this study was to grow human ameloblast-like epithelial cells in culture. DESIGN:Human fetal tooth organs were isolated, and the cells were separated by digestion in collagenase/...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.12.008
更新日期：2005-08-01 00:00:00
abstract：OBJECTIVE:Despite the targeted and incidental exposure of the oral cavity to probiotic bacteria, relatively little information is available concerning their bacteriological effects or ecological fate in this environment. We have investigated the effects of an oral probiotic bacterium, Lactobacillus reuteri on salivary ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.04.004
更新日期：2011-11-01 00:00:00
abstract：:The localization of cathepsin L and rat salivary cystatin-3 (RSC-3) in rat osteoclasts (rat femoral and alveolar bones) treated with or without E-64 (control) was examined immunocytochemically. In osteoclasts pretreated with E-64, immunoreactivity for cathepsin L was very weak extracellularly compared to that in the c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00003-4
更新日期：1997-04-01 00:00:00
abstract：:Sample groups of children (n=50) and adults (n=38) were selected from pools of 207 children, (11-13-year olds from two primary schools) and 94 adults (25-44-year olds from four governmental agencies) who were the subjects of an oral health survey among Tibetans living in Lhasa, Tibet Autonomous Region. Mean ages of th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00201-7
更新日期：2003-02-01 00:00:00
abstract：:It is well accepted that reduced masticatory function induced by a diet with soft physical consistency causes alterations in the craniofacial morphology in growing animals. It is assumed that these alterations are associated with reduced proliferative activity of osteoblasts on the bone surface, indicating a significa...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00057-1
更新日期：2000-10-01 00:00:00
abstract：OBJECTIVE:Interleukin (IL)-12 is an important cytokine for innate and adaptive immunity. We previously reported that IL-12 inhibits tumour necrosis factor (TNF)-α-mediated osteoclast formation by inducing apoptosis. We also reported that TNF-α plays an important role in mechanical loading-induced osteoclast formation a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.07.006
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:Respiratory epithelial cells are the first natural barrier against bacteria and viruses; hence, the interactions among epithelial cells, bacteria, and viruses are associated with disease occurrence and development. The effect of co-infection by P. gingivalis and influenza A virus (IAV) on respiratory epitheli...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.04.003
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:Sophora flavescens AITON (Leguminosae) is a typical traditional Korean medical herb considered to exhibit antibacterial, anti-inflammatory, and antipyretic effects, and is also used for the treatment of skin and mucosal ulcers, sores, diarrhea, gastrointestinal hemorrhage, arrhythmia, and eczema. OBJECTIVE ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.04.001
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE:Biomarkers in gingival crevicular fluid (GCF) have been investigated; however, measurements were limited by the small sample volume available. The aim of this study was to determine the levels of 40 different cytokines and chemokines in GCF samples. DESIGN:Eleven patients with generalised chronic periodontit...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.11.012
更新日期：2013-06-01 00:00:00
abstract：:Designing a temporomandibular joint (TMJ) total prosthesis requires the assessment of joint displacements for open/close movements. Current knowledge presents disc-condyle motions as involving both translation and rotation but there may be substantial variations from human to human. The aim of this study is to discrim...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.11.010
更新日期：2008-05-01 00:00:00
abstract：:Porphyromonas gingivalis culture products and a purified trypsin-like protease (TLPase) from the organism were tested for their effects on the phagocytosis of P. gingivalis by polymorphonuclear leucocytes (PMN) from 16 patients with adult periodontitis and 16 healthy subjects in a case-control study. Both the culture ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90134-8
更新日期：1993-04-01 00:00:00
abstract：OBJECTIVE:Eight commercially available resins, Epoxy Embedding Medium ("Epon"), Spurr, Epofix, Epo-Kwick, Epo-Thin, Technovit 9100 New, Unicryl, and Vestopal W, all intended for embedding specimens for microscopy, were tested and compared for binding and adaptation to the enamel surface of human third molar crowns that...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00194-8
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVE:It has been suggested that oxidative stress plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD), though this role has yet to be fully elucidated. The purpose of this study was to further evaluate this role as concomitantly expressed in the saliva and broncho-alveolar lavage (BAL/'...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.08.002
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease characterized by permanent cartilage loss. Articular cartilage is maintained in a low-oxygen environment. The chondrocyte response to hypoxic conditions involves expression of hypoxia inducible factor 1α (HIF-1α), which induces chondroc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.10.028
更新日期：2017-01-01 00:00:00
abstract：:After single, oral doses, 8 h profiles of fluoride (F) concentrations in plasma were determined in healthy human volunteers. Bioavailability of F from bone-meal, calcium and CaF2 tablets was evaluated in relation to that of NaF. Tablets were administered either whole or as a finely-ground powder, either to fasting sub...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90074-4
更新日期：1987-01-01 00:00:00
abstract：:Intrusive displacement of 7 incisors was measured before and within 3 min of extraction. A substantial increase in intrusion with recovery from loads of 4 N followed replacement of the incisors. These changes are taken to indicate that tension is an important mechanism of support in the intact periodontal ligament. ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90057-2
更新日期：1991-01-01 00:00:00
abstract：:Bone morphogenetic protein-7 (BMP-7) affects differentiation of preosteoblasts enabling the resultant cells to respond optimally to acutely acting regulators. As the phosphoinositide cascade and, particularly, the calcium-mobilizing inositol 1,4,5-trisphosphate (InsP3) receptor are integral to stimulus-secretion coupl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00122-3
更新日期：2000-02-01 00:00:00
abstract：INTRODUCTION:Intact neural supply is necessary for tooth eruption. Sympathetic denervation accelerates or decelerates the eruption rate depending on the tooth condition (intact or injured). The aim of this study is to reexamine the role of the sympathetic innervation, through the observation of the effects of pre or po...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.02.003
更新日期：2018-05-01 00:00:00
abstract：:The periodontal ligament (PDL) contains precursor cells for osteoblasts and cementoblasts. It has been shown that epidermal growth factor (EGF) inhibits dexamethasone-induced differentiation and up-regulates EGF-receptor (EGF-R) expression, whereas EGF-R is down-regulated in the course of differentiation. Thus it was ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(98)00079-x
更新日期：1998-12-01 00:00:00
abstract：:The morphology and distribution of various types of intercellular junctions were investigated in young odontoblasts. Gap junctions were found between odontoblasts as well as between odontoblasts and fibroblasts in the dental pulp. The junctions between odontoblasts were larger and more numerous than those between odon...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90068-2
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVES:RhoE and regulator of G-proteins signalling (RGS) 2 were identified as the up-regulated genes in human periodontal ligament (PDL) cells under compression. RhoE belongs to the Rho GTPase family, and RGS2, a novel family of GTPase-activating proteins, turns off the G-protein signalling. Rho family proteins hav...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.11.010
更新日期：2014-02-01 00:00:00
abstract：:Significant differences were found in the electromyographic (EMG) activation between the masseter and temporalis muscles for the leaf gauge (LG), manually manipulated (CR) and neuromuscular (NM) bite positions during maximal static clench. The LG position consistently demonstrated the lowest EMG activity, while the NM...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90037-m
更新日期：1993-03-01 00:00:00
abstract：OBJECTIVE:Recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. The purpose of the p...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.01.010
更新日期：2009-04-01 00:00:00
abstract：:The net surface charge of various oral streptococci were assessed by aqueous two-phase partitioning in a dextran-polyethylene glycol system. Great variability was found among individual strains within all species tested. Type 1 strains of Streptococcus sanguis serotypes which have been found to be more adherent, expos...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(81)90114-x
更新日期：1981-01-01 00:00:00
abstract：:Smears of buccal mucosa, dorsal surface of the tongue and floor of mouth were taken from 10 patients with histologically confirmed oral lichen planus and 12 healthy age- and sex-matched controls. In buccal smears, no significant differences in cytoplasmic and nuclear areas were observed between lesional, adjacent non-...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00106-9
更新日期：1996-01-01 00:00:00
abstract：:Electrical stimuli at 1 Hz with pulse widths of 0.05, 0.1 and 1 ms with intensities from two to six times sensory threshold (2-6 T) were delivered to the lower lip. The reflex responses were monitored by surface electromyography of the ipsilateral masseter muscle. An excitatory response that was not preceded or follow...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90097-3
更新日期：1994-08-01 00:00:00
abstract：OBJECTIVE:To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. METHODS:S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. U...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.01.004
更新日期：2018-03-01 00:00:00
abstract：:Albumin and alpha 2-macroglobulin were analysed in gingival fluid collected by filter paper strips from 6 volunteers before, during and after 10 days of dental plaque accumulation. The two proteins were also determined in samples of venous blood at the beginning of abstention from tooth brushing and 10 days later. A n...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90107-l
更新日期：1990-01-01 00:00:00
abstract：:The acquired enamel pellicle formed by selective adsorption of proteins in whole saliva is a protective integument on the tooth surface. The purpose of the present study was to investigate the formation of human acquired enamel pellicle using an in vitro hydroxyapatite (HA) model and 3H-histatin 5 to allow accurate me...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.06.003
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:A loss of functional salivary glands often occurs after radiotherapy for head and neck tumour, and causes many problems in oral health. Recently, the use of salispheres, which consist of salivary gland stem cells (SGSCs), has been suggested as therapy for these problems. However, an insufficient number of cel...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.03.003
更新日期：2015-06-01 00:00:00
