Abstract:
BACKGROUND:Sophora flavescens AITON (Leguminosae) is a typical traditional Korean medical herb considered to exhibit antibacterial, anti-inflammatory, and antipyretic effects, and is also used for the treatment of skin and mucosal ulcers, sores, diarrhea, gastrointestinal hemorrhage, arrhythmia, and eczema. OBJECTIVE AND DESIGN:This study examined the inhibitory effects of sophoraflavanone G (SF) of S. flavescens on the bacterial fibrillar protein, Antigen I/II (AgI/II)-N recombinant protein isolated from Streptococcus mutans(rAg I/II)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2). The investigation was focused on whether SF could inhibit the production of proinflammatory mediators such as nitric oxide (NO) and prostaglandin (PG) E2 as well as the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-a, interleukin (IL)-6, nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) in rAgI/II-stimulated RAW 264.7 cells using Griess reagent, Enzyme linked immunosorbent assay (ELISA), and Western blotting analysis. RESULTS:SG significantly inhibited the production of NO and PGE2 and pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor α in Ag I/II-N-stimulated RAW264.7 cells, which were mediated by the down-regulation of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. The SF inhibited the phosphorylation of IκB-α, nuclear translocation of p65, and subsequent activation of NF- κB in the rAgI/II-stimulated cells. In addition, the SF suppressed the rAgI/II-stimulated activation of ERK MAPK as well as the MAPK inhibitor significantly reduced the rAgI/II-induced production of NO and PGE2. CONCLUSION:Collectively, we suggest that the SF inhibits the expression and production of inflammatory mediators by blocking the ERK MAPK mediated pathway and inhibiting the activation of NF-κB.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Cha SM,Cha JD,Jang EJ,Kim GU,Lee KYdoi
10.1016/j.archoralbio.2016.04.001subject
Has Abstractpub_date
2016-08-01 00:00:00pages
97-104eissn
0003-9969issn
1879-1506pii
S0003-9969(16)30086-3journal_volume
68pub_type
杂志文章abstract::Porphyromonas gingivalis (P. gingivalis) is one of the prominent periodontal pathogens and is the most important bacteria involved in the onset and exacerbation of periodontitis. P. gingivalis is an anaerobic, Gram-negative coccobacillus that plays a role in the progression of periodontal disease by promoting alveolar...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.02.012
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:The flower of chrysanthemum, used worldwide as a medicinal and edible product, has shown various bioactivities, such as anti-inflammatory, antioxidant, anti-tumorigenic, and hepatoprotective activities, as well as cardiovascular protection. However, the effect of Chrysanthemum morifolium Ramat. on the regulat...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.105029
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abstract::Ascorbic acid (AA), also known as Vitamin C, is a cofactor required for the function of several hydroxylases. It is not synthesised in humans and has to be provided by diet. Its absence is responsible for scurvy, a condition related to the defective synthesis of collagen by the reduced function of prolylhydroxylase. A...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.11.006
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVE:To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. METHODS:S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. U...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.01.004
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abstract:OBJECTIVE:To compare the densities of CD1a + immature and CD83+ mature dendritic cells, and inflammatory infiltrate cells between smokers and non-smokers with oral leukoplakia. Parameters associated with malignant transformation were also evaluated. DESIGN:21 smokers and 23 non-smokers diagnosed with oral leukoplakia ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.07.008
更新日期:2018-11-01 00:00:00
abstract::Twenty-nine parotids ligated for between 1 and 365 days were examined by light and electron microscopy. Major changes in the acini were seen at 4 days and included vacuolation, disintegration, extravasation, apoptosis, phagy and a reduction in number and size of secretory granules. There was a further reduction in sec...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00076-5
更新日期:2000-11-01 00:00:00
abstract::Fluorescently labelled latex microspheres (0.01, 0.1 and 1.0 micron dia.) were used to establish whether oral epithelial cells could exhibit an endocytic function. Oral mucosa biopsies were incubated in organ culture at 37 degrees C for 20 h with one of the three sizes of fluorescent microspheres in the medium. Tissue...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00027-8
更新日期:1999-06-01 00:00:00
abstract::An engineering model of the periodontal ligament fibres was used to calculate length changes in the fibres with: direct occlusal forces on the tooth, occlusal loads on other teeth in the arch and splinting. The fibre elongations appear to influence tooth eruption, the clinical behaviour of splinted and non-splinted te...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90109-4
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to identify candidate genes as potential biomarkers in nasopharyngeal carcinoma (NPC) by bioinformatical analysis. METHODS:Three microarray datasets: GSE32906, GSE15170, GSE53819 were download from public database and analyzed to identify the differentially expressed genes (DEGs) between NPC...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.07.003
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Wnt5a is generally considered a non-canonical Wnt family member and plays an important role in the development of several tissues through regulation of cell fate, proliferation, migration, polarity and death. This study investigates its expression mode in human tooth development and the involved cell signal t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2009.12.002
更新日期:2010-02-01 00:00:00
abstract::After single, oral doses, 8 h profiles of fluoride (F) concentrations in plasma were determined in healthy human volunteers. Bioavailability of F from bone-meal, calcium and CaF2 tablets was evaluated in relation to that of NaF. Tablets were administered either whole or as a finely-ground powder, either to fasting sub...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90074-4
更新日期:1987-01-01 00:00:00
abstract::Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00038-q
更新日期:1995-08-01 00:00:00
abstract::Porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with Actinomyces viscosus cells. A 40-kDa outer membrane protein (OMP)-coding gene from P. gingivalis was cloned and the protein was found to be localized in these vesicles. The recombinant 40-kDa OMP did n...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90078-m
更新日期:1992-09-01 00:00:00
abstract::An immunofluorescence study of the expression of keratin 14 (K14) during the formation of filiform papillae was performed and the progress of keratinization of the epithelium of the rat tongue was monitored on semi-ultrathin sections by laser-scanning microscopy. Differential interference contrast (DIC) images were al...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(03)00118-3
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVE:Changes in the masticatory function of ferrets have been shown to cause alterations in their craniofacial morphology. The aim of the present study was to investigate how the functional changes influence the histochemical characteristics of fibres of the masticatory muscles of the animal. DESIGN:Forty five-we...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.07.011
更新日期:2004-12-01 00:00:00
abstract::The number of Strep. mutans in saliva samples was examined in 101 13-14-year-old children, 53 in a control and 48 in the test group. All in the test group with 2.5 X 10(5) Strep. mutans per ml saliva were treated with 1 per cent chlorhexidine gel, once a day, for 14 days when the number of Strep. mutans was greatly re...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90042-5
更新日期:1982-01-01 00:00:00
abstract::A water-soluble extract of Actinomyces viscosus (AVS) was tested for its capacity to induce DNA synthesis in lymphocytes from man, monkeys, mice and guinea pigs. The results indicated that the AVS induced an in-vitro lymphoproliferative response, as assessed by tritiated thymidine incorporation, in mouse-spleen cells,...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90161-3
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to investigate the expression of pannexin3 (Panx3) in human odontoblast-like cells (hOBs) and its hemichannel function in mediating ATP release. METHODS:RT-PCR and immunofluorescence analysis were used to detect the expression of pannexins (Panxs) in human dental pulp tissue and cult...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.07.005
更新日期:2015-10-01 00:00:00
abstract::Covalent intermolecular cross-links in collagen provide the dentine matrix with stability and tensile strength. The density of collagen cross-links varies depending on the site within the same tissue. This variation is probably due to factors such as the different amounts of stress and different turnover rates at the ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90118-6
更新日期:1993-07-01 00:00:00
abstract::Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00160-7
更新日期:2003-01-01 00:00:00
abstract::The rate of synthesis of secretory proteins increases significantly in rat parotid glands after stimulated discharge of stored proteins. How any difference in the amount of secretory protein discharge affects the rate of subsequent protein synthesis, and whether this post-secretory synthesis is regulated at the level ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90147-7
更新日期:1989-01-01 00:00:00
abstract::Secretory-stage inner dental epithelial cells (IDE) of tooth buds deposited an unmineralized, ectodermally-derived, enameloid collagen matrix. Pharyngeal plates bearing tooth buds were fixed: some were demineralized, others treated with guanidine-EDTA, then fixed and post-fixed in osmium tetroxide with potassium ferri...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90030-0
更新日期:1986-01-01 00:00:00
abstract::Samples of dentine from normal deciduous and permanent teeth were demineralized with EDTA and cleaved with CNBr. The resulting peptides were separated by means of electrophoresis on polyacrylamide slab gels, stained with Coomassie blue and examined by transmitted light. Final identification was performed by means of a...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90124-9
更新日期:1984-01-01 00:00:00
abstract:AIM:This study was planned to investigate whether smoker patients with inflammatory periodontal disease exhibit different salivary concentrations of C-telopeptide pyridinoline cross-links of type I collagen (ICTP) and osteocalcin (OC) compared to the non-smoker and/or ex-smoker counterparts. METHODS:Whole saliva sampl...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2009.09.009
更新日期:2009-12-01 00:00:00
abstract::Chronic snuff dipping has been associated with oral cancer in man and experimental animals. Here, the effects of a water-extract of snuff on the in-vitro development of human lymphokine-activated killer (LAK) activity were examined. The snuff extract inhibited both LAK cytotoxicity and DNA synthesis in a dose-dependen...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90064-7
更新日期:1988-01-01 00:00:00
abstract::Enamel and cementum pellicles form by different adsorption of salivary and serum components to the tooth surface. The authors compared the constituents of surface pellicle formed on human enamel and cementum under three conditions: (1) natural pellicle, present on extracted teeth, which was formed by prolonged exposur...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(87)80013-4
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to identify the optimal micro- and submicroscale topographies of titanium (Ti) substrata that would most significantly influence adhesion, proliferation, and other activities of these cells. DESIGN:Truncated V-shaped microgrooves in 60 μm-wide and 10 μm-deep cross-sections with 0°, 30°, 60°,...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.11.013
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to assess the frequency of variations in tooth number, shape and position in the dental arch to determine if there are parts of the dental arch that show a higher prevalence of tooth variability. DESIGN:198 randomly selected Caucasian subjects (93 females, 105 males) aged between 10...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.11.009
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:Despite the targeted and incidental exposure of the oral cavity to probiotic bacteria, relatively little information is available concerning their bacteriological effects or ecological fate in this environment. We have investigated the effects of an oral probiotic bacterium, Lactobacillus reuteri on salivary ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.04.004
更新日期:2011-11-01 00:00:00
abstract::Dental follicle has been implicated as the origin of alveolar bone, cementum and periodontal ligament, but there is no direct evidence of their cellular lineage. The present pilot study was designed to characterize the phenotype of cultured cells obtained from the dental follicle of neonatal mouse molars. Developing m...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00033-3
更新日期:1999-09-01 00:00:00