Abstract：
:Fluorescently labelled latex microspheres (0.01, 0.1 and 1.0 micron dia.) were used to establish whether oral epithelial cells could exhibit an endocytic function. Oral mucosa biopsies were incubated in organ culture at 37 degrees C for 20 h with one of the three sizes of fluorescent microspheres in the medium. Tissue pieces were then disaggregated and cell suspensions analysed for cell content and viability. Evidence of endocytosis was sought by using fluorescence-activated cell scanning (FACS) and confocal microscopy to study the epithelial cell suspensions for internalization of the microspheres. Confirmation that the microspheres had been internalized and were not merely attached to the cell exterior was shown by using trypan blue quenching to extinguish extracellular fluorescence, allowing analysis of only intracellular fluorescent microspheres. Both FACS and confocal microscopy confirmed uptake of 0.01 and 0.1 micron dia. microspheres but not 1.0 micron. Endocytosis was quantitated using FACS and a dose-dependent relation between the concentration of spheres in the incubation medium and uptake was found. Internalization of microspheres of < 1.0 micron dia. and the dose-dependent uptake support a fluid-phase constitutive endocytic process.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Innes NP,Ogden GRdoi
10.1016/s0003-9969(99)00027-8subject
Has Abstractpub_date
1999-06-01 00:00:00pages
519-23issue
6eissn
0003-9969issn
1879-1506pii
S0003-9969(99)00027-8journal_volume
44pub_type
杂志文章abstract：OBJECTIVE:To compare the tissue reactions to implanted collagen scaffolds in the palate and the skin of rats. DESIGN:Crosslinked collagen scaffolds were implanted submucoperiosteally in the palate, and subcutaneously on the skull and on the back of 25 rats and evaluated after up to 16 weeks. On H&E-stained sections, t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.11.003
更新日期：2008-04-01 00:00:00
abstract：:Destrin, an actin-binding protein, is partly phosphorylated at Ser-2 (numbering on the matured form) in the resting rat parotid gland, and beta-adrenergic or cholinergic stimulation of this gland induces its dephosphorylation. In this study, we searched for the protein kinase involved in phosphorylation of destrin. We...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00129-8
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:To determine if dentine at the enamel-dentine junction (EDJ) in man is more sensitive to hydrostatic pressure stimuli then deeper dentine. DESIGN:Cavities (1mm diam.) were cut at the tips of the buccal and lingual cusps of 8 premolars in 3 subjects (ages: 22-25 years). Both cavities were initially deepened t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.02.015
更新日期：2016-06-01 00:00:00
abstract：:The aim of this project was to develop an analytical procedure with the required level of sensitivity for the determination of glucose concentrations in small volumes of unstimulated fasting whole saliva. The technique involves high-performance ion-exchange chromatography at high pH and pulsed amperometric detection. ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00121-2
更新日期：1996-02-01 00:00:00
abstract：:Eight primary incisors obtained from a child with dentinogenesis imperfecta (DI) type II were examined histologically using light microscopy and scanning electron microscopy. In both the DI and control teeth, large dentinal canals were observed along the midline of the crown distributed mesial-distally and coursing to...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90116-5
更新日期：1985-01-01 00:00:00
abstract：OBJECTIVE:Gingival fibroblasts (GFs) can participate in the immune response and play an immune-regulatory role. This study was to evaluate whether GFs can be differently activated and polarized into distinct functional subtypes in response to T helper type 1 (Th1), or Th2 stimuli. DESIGN:Human GFs were stimulated by t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.04.019
更新日期：2019-06-01 00:00:00
abstract：:Histatins 1, 3 and 5 are the major members of a histidine-rich protein family present in human salivary secretions. These proteins are distinct from many salivary proteins in their high positive charge density at neutral pH, and their antibacterial and antifungal properties. In this study, the hydroxyapatite adsorptio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00012-8
更新日期：2003-05-01 00:00:00
abstract：OBJECTIVE:Increasing attention has been given to the potential protective roles of specific antioxidant nutrients found in fruits and vegetables. However, there are relatively few reports on the cancer chemopreventive effects of lycopene or tomato carotenoids in animal models. Therefore, the chemopreventive efficacy of...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.12.007
更新日期：2011-07-01 00:00:00
abstract：:The effect of chronic mandibular protrusion on the collagenolytic and phosphatase activity of several mandibular bone sites and the condylar cartilage was evaluated. Ninety-three male Sprague-Dawley rats were equally divided into two experimental and one control group. One experimental group wore a protrusive applianc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90067-8
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:Our goal was to study the development of pioneer sympathetic innervation of dental pulp of mouse mandibular first molar. DESIGN:We used double fluorescent immunohistochemistry with tyrosine hydroxylase (TH) and anti-medium-chain neurofilament (2H3) antibodies to detect sympathetic and sensory nerve fibres. S...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.03.004
更新日期：2008-09-01 00:00:00
abstract：:The recovery of fibres in the chorda tympani after repair by epineurial suture or entubulation was investigated. The combined trunk of the chorda tympani and lingual branch of the trigeminal nerve was sectioned unilaterally, repaired using either epineurial sutures or entubulation, and allowed to recover for 12 weeks....
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)00189-i
更新日期：1995-05-01 00:00:00
abstract：:Human epithelial cells isolated from adult gingival and infant palatal biopsies were cultured using 3T3 feeder cells. The colony-forming efficiency was about 0.8 per cent with cholera toxin and epidermal growth factor (EGF). The cell yield of cultures from infant palates depended on the concentration of cholera toxin ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90113-9
更新日期：1983-01-01 00:00:00
abstract：:A variable capacitance displacement transducer, previously employed for continuously monitoring movements of the rabbit mandibular incisor, was modified for use with the ferret mandibular canine. Comparing this tooth of limited growth with the tooth of continuous growth, similarities were seen in the reactions to extr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90177-2
更新日期：1983-01-01 00:00:00
abstract：:The need for a rapid and sensitive microbiological assay has become necessary for both research and clinical diagnostic purposes. This need has become clear as a result of extensive documentation linking specific bacterial species and periodontal destruction. DNA probe technology provides both a sensitive and specific...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90147-3
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:Streptococcus mutans, known to be a major pathogen of dental caries, is also considered to cause infective endocarditis. Its 120-kDa Cnm protein binds to type I collagen, which may be a potential virulence factor. In this study, we characterized S. mutans clinical strains focusing on the cnm gene encoding Cnm...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.11.008
更新日期：2010-01-01 00:00:00
abstract：:The peroxidase activity in human whole saliva is due to salivary peroxidase and, in some cases, myeloperoxidase; it is usually determined by spectrophotometric methods based on the rate of oxidation of chromogen substrates. Thiocyanate ion, a normal component of saliva, interferes with these kinetic assays by competin...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90095-6
更新日期：1986-01-01 00:00:00
abstract：:This report concerns changes brought about in the submandibular salivary gland during different stages of the oestrous cycle in the concentrations of total lipids, cholesterol and protein, together with an assessment of the activities of cyclic AMP-specific phosphodiesterase, ATPase, succinate dehydrogenase and an est...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(96)00011-8
更新日期：1996-02-01 00:00:00
abstract：OBJECTIVE:Chondrocyte apoptosis is a pathological manifestation of osteoarthritis. The goal of this report was to explore the role of nitric oxide in chondrocyte apoptosis in osteoarthritic mandibular condylar cartilage. DESIGN:This study used our reported experimental unilateral anterior crossbite in vivo rat model a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.03.006
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:To test the association of child's growth/development status and socioeconomic variables with dental emergence patterns. DESIGN:A sample of 359 children belonging to a birth cohort of over 5000 children born in 1993 in Pelotas (Brazil) was studied in 1998-1999. Previously trained observers collected birthwei...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.12.001
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVE:This study evaluated changes in protein profile of the acquired enamel pellicle (AEP) formed in vivo, after application of gels containing chlorhexidine or EGCG and further challenge with citric acid. DESIGN:AEP was formed in 9 volunteers for 2h and then treated with one of the following gels: placebo, 400μM...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.05.024
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to analyze the characteristics of the clustered regularly interspaced short palindromic repeats (CRISPR) sites in 45 clinical Streptococcus mutans strains and their relationship to the clinical manifestations of early childhood caries (ECC). METHODS:Forty-five S. mutans strains were...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.07.023
更新日期：2017-11-01 00:00:00
abstract：:This study was undertaken to reveal detailed changes in fluoride distribution at different developmental stages of upper incisor enamel under various fluoride administration regimes. Four groups of Wistar rats received water containing 0, 25, 50 and 100 parts/10(6) fluoride respectively for 10 weeks. Five different en...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90119-7
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:Dental follicle cells (DFCs) are the genuine precursors of alveolar osteoblasts. Previous studies suggested that collagen I supports the osteogenic differentiation of DFCs. This study investigated the effect of collagen I on the osteogenic differentiation of human DFCs. MATERIALS AND METHODS:We modified the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.07.013
更新日期：2014-12-01 00:00:00
abstract：:The biological activity of carboxyl-terminal parathyroid hormone peptide[53-84] (PTH[53-84]) and aminoterminal peptide [1-34] (PTH[1-34]) during cultivation of the lower molar tooth germ from mouse embryos was examined. PTH[1-34] increased alkaline phosphatase activity in the tooth germ at an early developmental stage...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00107-6
更新日期：1998-04-01 00:00:00
abstract：OBJECTIVE:To understand bone regeneration process after tooth extraction could be a clue to develop a new strategy for alveolar bone reconstruction. Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. In this study, we investigated the spat...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00153-5
更新日期：2003-10-01 00:00:00
abstract：OBJECTIVES:Dental evolution affects the geometry of the tooth, but the adaptive relevance of these changes is related to tooth sharpness, complexity, and relief (topography). On a set of laboratory mice, we assessed how wear related to age and food consistency affected molar geometry and topography. DESIGN:Three group...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.028
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVE:We previously proposed the hamster cheek pouch model of oral cancer for BNCT studies. We herein present the biodistribution of a non-toxic boron compound, GB-10 (Na(2)(10)B10H10), in this model to assess its potential for BNCT or BNCT enhanced Fast Neutron Therapy. MATERIALS AND METHODS:We evaluated the upta...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2003.10.003
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:The effect of experimental jaw-muscle pain and stress on masticatory muscle activity in TMD-patients has been discussed. Furthermore, associations between TMD and deep bite patients have been studied. Accordingly in the present study, comparison of EMG responses at rest, maximal clenching, during evoked pain ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.07.003
更新日期：2013-10-01 00:00:00
abstract：:Electromyographic recordings (EMG) obtained from intramuscular electrodes inserted in the lateral pterygoid muscle (lat. pt.) of 29 juvenile and adolescent monkeys were assessed in relation to the recorded activity of three jaw elevating muscles, temporalis, masseter and medial pterygoid, and a jaw muscle depressor, t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90087-5
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:MicroRNAs (miRNAs) are recognised as important regulators of a variety of fundamental biologic processes. Our study was undertaken to examine the role of MicroRNA-203 (miR-203) in modulating nitric oxide (NO) expression in female Sprague-Dawley rat mandibular condylar chondrocytes (MCCs) via targeting transie...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.08.013
更新日期：2013-02-01 00:00:00
