Abstract：
:The localization of cathepsin L and rat salivary cystatin-3 (RSC-3) in rat osteoclasts (rat femoral and alveolar bones) treated with or without E-64 (control) was examined immunocytochemically. In osteoclasts pretreated with E-64, immunoreactivity for cathepsin L was very weak extracellularly compared to that in the control osteoclasts. However, it was strong intracellularly. The localization of RSC-3 was unclear in the control osteoclasts, while in E-64 treated osteoclasts, both the clear zone and ruffled border areas showed a very strong immunoreaction. At the electron-microscopic level, in normal osteoclasts, numerous immunoreaction products for cathepsin L were found extracellularly in the bone matrix under the ruffled border, while few intracellular products were observed. In contrast, in the E-64-treated osteoclasts, only a few immunoreaction products were found extracellularly, while intracellularly cathepsin L was found in numerous endosome-lysosomal vacuoles. In the immunoreaction for RSC-3, the cytoplasm of the ruffled border was positive, and the tips of the RSC-3-positive ruffled border appeared to enter deeply into the bone matrix. Intracellularly, the granular reaction products of RSC-3 were found in the vacuoles (probably autophagolysosomes). Thus, in E-64-treated osteoclasts, inhibition of the extracellular release of cathepsin L was demonstrated. In addition, intralysosomal accumulation of RSC-3 and deep penetration of the RSC-3-positive ruffled border into the bone matrix were found. These findings suggest that RSC-3 is associated with the inhibition of cathepsin L in both the lysosomes (in the osteoclasts) and bone matrix.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Moroi R,Yamaza T,Nishiura T,Nishimura Y,Terada Y,Abe K,Himeno M,Tanaka Tdoi
10.1016/s0003-9969(97)00003-4subject
Has Abstractpub_date
1997-04-01 00:00:00pages
305-15issue
4eissn
0003-9969issn
1879-1506pii
S0003-9969(97)00003-4journal_volume
42pub_type
杂志文章abstract：OBJECTIVE:Oral lichen planus (OLP) is a disease of the oral mucosa of unknown cause producing lesions with an intense band-like inflammatory infiltrate of T cells to the subepithelium and keratinocyte cell death. We performed gene expression analysis of the oral epithelium of lesions in subjects with OLP and its sister...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.12.010
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVES:The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival ...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2017.12.022
更新日期：2018-03-01 00:00:00
abstract：:Apoptosis in regressive primary (deciduous) dental primordia was examined in the embryos of Suncus murinus, which is a monophyodont. The primary tooth germs of S. murinus are temporarily formed and disappear during the embryonic period before they are calcified. Most primary tooth germs reach the bell stage and degene...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00007-3
更新日期：2001-07-01 00:00:00
abstract：:The need for a rapid and sensitive microbiological assay has become necessary for both research and clinical diagnostic purposes. This need has become clear as a result of extensive documentation linking specific bacterial species and periodontal destruction. DNA probe technology provides both a sensitive and specific...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90147-3
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:MicroRNAs (miRNAs) are recognised as important regulators of a variety of fundamental biologic processes. Our study was undertaken to examine the role of MicroRNA-203 (miR-203) in modulating nitric oxide (NO) expression in female Sprague-Dawley rat mandibular condylar chondrocytes (MCCs) via targeting transie...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.08.013
更新日期：2013-02-01 00:00:00
abstract：:For the rate of demineralization of enamel during caries, three physicochemical processes are of importance: the diffusion into and out of the enamel, the dissolution of mineral and the complexation of ions. The interpretation of diffusion experiments in conjunction with artificial caries experiments and computer simu...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/0003-9969(83)90006-7
更新日期：1983-01-01 00:00:00
abstract：:This study sought to determine if the diet-induced changes in submandibular salivary glands can be reversed. Two groups of male weanling Sprague-Dawley rats were fed purified diets containing 9% butter + 1% corn oil (group I, control) or 9% ethyl ester concentrate of n-3 fatty acids + 1% corn oil (group II, experiment...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90209-5
更新日期：1993-05-01 00:00:00
abstract：:Soluble reduced lysozyme was extensively digested by a trypsin-like protease purified from the culture supernatant of the bacterium. The digestion peptides were separated and purified by reversed-phase high-performance liquid chromatography, and were subjected to amino acid analysis. The fragments were identified by t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90149-0
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:To determine whether chewing side preference is related to handedness and lateral asymmetry of occlusal characteristics, muscular force and temporomandibular disorders (TMD) in a dentate population. DESIGN:One hundred and seventeen dentate adults participated in this cross-sectional study. Static and dynamic...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.09.006
更新日期：2009-02-01 00:00:00
abstract：:The purpose of this study is to investigate the radioprotective effect of vitamin E as a natural product. Vitamin E protects the salivary glands dysfunction that is induced by ionizing radiation. It was analysed with radioisotope scintigraphy and then salivary gland to background counts ratio was calculated. Histopath...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.06.003
更新日期：2015-09-01 00:00:00
abstract：:Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00051-9
更新日期：1996-08-01 00:00:00
abstract：:Eruption results in the movement of the tooth from an intraosseous to a functional position in the mouth. This physiological movement occurs through a path of least resistance and is mediated by the dental follicle. In this study the presence of a 80-kDa proteinase of the gelatinase family in the dental follicle of ma...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)00168-b
更新日期：1995-04-01 00:00:00
abstract：OBJECTIVE:Low dose propranolol has previously been demonstrated to suppress bone remodelling. Therefore, its effect on orthodontic movement was tested. DESIGN:Rats were assigned as follows (n=5): animals with no orthodontic appliance (G1); the remaining groups were fitted with a Ni-Ti closed-coil spring ligated to the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.06.006
更新日期：2014-10-01 00:00:00
abstract：AIM:The aim of the present study was to systematically review the influence of orthodontic force on human dental pulp. METHODS AND RESULTS:The addressed focused question was "Do orthodontic forces affect the human dental pulp?" which was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2014.11.011
更新日期：2015-02-01 00:00:00
abstract：:Ruthenium hexammine trichloride (RHT) used as a probe to visualize anion groups in the predentine and dentine of rat incisors, showed a complex distribution pattern including: intercellular proteoglycans (Pg), detected in the predentine as granules 10-15 nm in diameter and as filaments. Non-aggregating Pg was observed...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90051-8
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVE:The focus of the current study was to identify if a possible association between NLRP3 (rs4612666) and IL-1B (rs1143634) single-nucleotide polymorphisms (SNPs) may be implicated in the etiopathogenesis of chronic periodontitis (CP) in a Colombian population. DESIGN:One hundred and twenty-four CP subjects and...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.01.013
更新日期：2016-05-01 00:00:00
abstract：:Salivary gland intralobular ducts are responsible for the modification of the electrolyte composition of the primary fluid secreted by the acini. However, the intracellular messengers that regulate this and other intralobular duct cell processes have not been fully characterized. To investigate the possibility that cA...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00028-3
更新日期：1996-07-01 00:00:00
abstract：:The histochemical muscle-fibre composition of the anterior and posterior belly of the human digastric muscle was analysed in young male adults. Both bellies, of differing embryological origin and supplied by different cranial nerves, showed a fibre composition similar to that of normal limb and trunk muscles. Type I, ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90054-1
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:We aimed to differentiate dental pulp stem cells (DPSC) to odontoblast-like cells (ODPSC) and to investigate their attachment and growth on dentin in the presence of extra calcium by colorimetric assay and scanning electron microscopy (SEM). METHODS:After isolation of DPSC, they were differentiated to ODPSC....
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.04.008
更新日期：2016-08-01 00:00:00
abstract：:Sixteen consecutively-born monkeys were used in a longitudinal study to determine the changes occurring in the total number of bacteria in the palatal grooves of the upper left first deciduous molar teeth, following the introduction of a sucrose-based diet. The total number of bacteria recovered from the grooves incre...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90029-9
更新日期：1985-01-01 00:00:00
abstract：:The aim was to establish defined-species consortium plaque biofilms to investigate enamel and root caries in an artificial mouth. Strains of the putative enamel and root caries pathogens, Streptococcus mutans, Strep. sobrinus, Actinomyces naeslundii and Lactobacillus rhamnosus, were screened in batch culture for poten...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00111-9
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:The function of apoptosis and its regulation in odontoblasts remain unclear. In this study, we characterize the possible role of transforming growth factor (TGF)-beta 1 in the induction of apoptosis and the molecular mechanisms that mediate TGF-beta1-induced apoptosis in odontoblasts. METHODS:Annexin V/propi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.03.004
更新日期：2005-11-01 00:00:00
abstract：:Previous studies have demonstrated that low-magnitude, high-frequency (LMHF) mechanical vibrations (LM<1g; HF<20-90Hz) can benefit bone formation. However, the underlying mechanism is unclear. In this study, MC3T3-E1 cells were exposed to LMHF vibrations of different magnitudes to assess the effect of vibrations on β-...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.06.009
更新日期：2016-10-01 00:00:00
abstract：:Inhibitory jaw reflexes are believed to be important for protecting the teeth and temporo-mandibular structures from damage during sudden or forceful biting or mastication. Accordingly, alterations in these reflexes are sometimes implicated in aetiologies proposed for oro-facial pain syndromes, although the associatio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.02.012
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVE:This study was aimed to investigate the effects of microRNA-212-5p (miR-212-5p) and myeloid differentiation factor 88 (Myd88) in Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS)-challenged human periodontal ligament cells (PDLCs). METHODS:The levels of miR-212-5p and Myd88 in PDLCs were determ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104831
更新日期：2020-10-01 00:00:00
abstract：:Previous studies have reported that the abnormal development of the second deciduous molar in Down syndrome and cerebral palsy begins before birth. In view of these results we have turned our attention to the earlier stages of dental development in utero, represented by the primary canine, in order to see if we can id...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.04.011
更新日期：2007-10-01 00:00:00
abstract：:Samples of dentine from normal deciduous and permanent teeth were demineralized with EDTA and cleaved with CNBr. The resulting peptides were separated by means of electrophoresis on polyacrylamide slab gels, stained with Coomassie blue and examined by transmitted light. Final identification was performed by means of a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90124-9
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the spatio-temporal relationships between articulators in the anterior oral cavity, during the production of Japanese fricative and plosive articulation using our proposed method for tooth visualization in MR image sequences. DESIGN:Ten healthy adults without malocclusion participated in the stud...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.11.002
更新日期：2012-06-01 00:00:00
abstract：:By ammonium-sulphate fractionation, phosphocellulose column chromatography, and isoelectric focusing, pyruvate kinase (EC 2.7.1.40) was purified from pig dental pulps to yield a homogeneous preparation with a single protein band of approx. 63,000 daltons upon SDS-polyacrylamide gel electrophoresis. As the molecular we...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90078-6
更新日期：1986-01-01 00:00:00
abstract：:Growth in body length, skeletal lengths, incisor lengths and molar root lengths were retarded in dwarf mice. The root lengths of third molars were more severely affected than those of other molars. The crown lengths at the enamel-cementum junction of upper and lower molars of most dwarf mice were significantly shorter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90135-9
更新日期：1985-01-01 00:00:00
