A 31P-NMR study on the energy state of rat liver in an experimental model of chronic dietary iron overload.

abstract：:Cathepsin D, a lysosomal aspartic proteinase, has been shown to induce apoptosis of HeLa cells when overexpressed. To further understand regulatory mechanisms of cathepsin D-induced cell death, we examined whether lysosomal cysteine and aspartic proteinases are involved in apoptosis of PC12 cells following serum depri...

journal_title：Biochemical and biophysical research communications

authors： Shibata M,Kanamori S,Isahara K,Ohsawa Y,Konishi A,Kametaka S,Watanabe T,Ebisu S,Ishido K,Kominami E,Uchiyama Y

更新日期：1998-10-09 00:00:00

abstract：:Vibrio cholerae cytolysin (VCC), a β-barrel pore-forming toxin (β-PFT), induces killing of the target eukaryotic cells by forming heptameric transmembrane β-barrel pores. Consistent with the β-PFT mode of action, binding of the VCC toxin monomers with the target cell membrane triggers formation of pre-pore oligomeric ...

journal_title：Biochemical and biophysical research communications

authors： Paul K,Chattopadhyay K

更新日期：2014-01-03 00:00:00

abstract：:Human embryonic stem cells (hESCs) are thought to be a promising cell source for cell transplantation therapy. For such a clinical application, the hESCs should be manipulated using appropriate and qualified materials. In this study, we examined the efficacy of recombinant human laminin (rhLM) isoforms on the undiffer...

journal_title：Biochemical and biophysical research communications

authors： Miyazaki T,Futaki S,Hasegawa K,Kawasaki M,Sanzen N,Hayashi M,Kawase E,Sekiguchi K,Nakatsuji N,Suemori H

更新日期：2008-10-10 00:00:00

abstract：:We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in...

journal_title：Biochemical and biophysical research communications

authors： Matsumoto H,Murakami Y,Horikoshi Y,Noguchi J,Habata Y,Kitada C,Hinuma S,Onda H,Fujino M

更新日期：1999-04-13 00:00:00

abstract：:The amino acid sequences in the NH2-terminal region and some other parts of human gastric cathepsin E were investigated. The NH2-terminal sequencing revealed that the cathepsin E preparation which had been activated at pH 4.0 contained one major and one minor isozymes in an approximate molar ratio of 3:1. The NH2-term...

journal_title：Biochemical and biophysical research communications

authors： Athauda SB,Matsuzaki O,Kageyama T,Takahashi K

更新日期：1990-04-30 00:00:00

abstract：:Stimulation of leucine uptake by addition of concanavalin A, mediated by increase of intracellular free Ca2+ concentration [( Ca2+]), in lymphocytes (Mitsumoto, Y., Sato, K. and Mohri, T. (1988) Biochim. Biophys. Acta 968, 353-358) was abolished by N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) and chlorprom...

journal_title：Biochemical and biophysical research communications

authors： Sato K,Mitsumoto Y,Mohri T

更新日期：1988-06-16 00:00:00

abstract：:Based on a previous finding that fusion of a somatic cell with an embryonic stem (ES) cell reprogrammed the somatic cell, genes for reprogramming transcription factors were selected and induced pluripotent stem (iPS) cell technology was developed. The cell fusion itself produced a tetraploid cell. To avoid nuclear fus...

journal_title：Biochemical and biophysical research communications

authors： Kim SM,Wada KI,Ueki M,Hosokawa K,Maeda M,Sakai Y,Ito Y

更新日期：2019-12-03 00:00:00

abstract：:Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within ...

journal_title：Biochemical and biophysical research communications

authors： Grubb DR,Wolvetang EJ,Lawen A

更新日期：1995-10-24 00:00:00

abstract：:12-O-tetradecanoyl phorbol-13-acetate-induced sequence 7/interferon related development regulator 1 (Tis7/IFRD1) has been recently identified as a modifier gene in lung inflammatory disease severity in patients with cystic fibrosis (CF), based upon its capacity to regulate inflammatory activities in neutrophils. In CF...

journal_title：Biochemical and biophysical research communications

authors： Blanchard E,Marie S,Riffault L,Bonora M,Tabary O,Clement A,Jacquot J

更新日期：2011-08-05 00:00:00

abstract：:Endogenous cysteine proteases were given much attention lately, as their role in a variety of pathophysiological disorders became evident. Amongst them cathepsins, which are thought to be implicated in mediation of osteoporosis, cancer progression, atherosclerosis, and many other conditions, are of considerable intere...

journal_title：Biochemical and biophysical research communications

authors： Bratkovic T,Lunder M,Popovic T,Kreft S,Turk B,Strukelj B,Urleb U

更新日期：2005-07-08 00:00:00

abstract：:The reaction of the crosslinker dibromobimane has recently revealed a functionally important internal loop structure within the skeletal myosin S1 heavy chain where Cys-522 of the 50K domain and Cys-707 (SH1) of the 20K region are spatially juxtaposed. Here we have studied the possible relevance of this topological fe...

journal_title：Biochemical and biophysical research communications

authors： Bonet A,Audemard E,Mornet D

更新日期：1988-04-15 00:00:00

abstract：:Here we demonstrate that the semi-synthetic flavonoid ayanin diacetate induces cell death selectively in leukemia cells without affecting the proliferation of normal lymphocytes. Incubation of human leukemia cells with ayanin diacetate induced G(2)-M phase cell cycle arrest and apoptosis which was prevented by the non...

journal_title：Biochemical and biophysical research communications

authors： Marrero MT,Estévez S,Negrín G,Quintana J,López M,Pérez FJ,Triana J,León F,Estévez F

更新日期：2012-11-09 00:00:00

abstract：:The tumor suppressor p53 is involved in cadmium (Cd)-induced apoptosis and autophagy. However, the regulatory mechanisms of p53 in Cd-induced kidney injury are not well established. Here, we report the role of autophagy in Cd-induced p53 induction in human proximal tubular cells (HK-2). HK-2 cells treated with Cd indu...

journal_title：Biochemical and biophysical research communications

authors： Lee HY,Oh SH

更新日期：2021-01-01 00:00:00

abstract：:We have screened genomic DNA for the identification of D4 dopamine receptor polymorphisms. We show that the D4 dopamine receptor genotype in 72 severely affected chronic alcoholics is heterogeneous, with individuals homozygous and heterozygous for the various D4 receptor alleles. Alcoholics demonstrated a greater prev...

journal_title：Biochemical and biophysical research communications

authors： George SR,Cheng R,Nguyen T,Israel Y,O'Dowd BF

更新日期：1993-10-15 00:00:00

abstract：:Ubiquitous calpains (calpain I and II) are generally recognized as cytosolic proteins. Recently, mitochondrial localized calpain I (μ-calpain) has been identified. Activation of mito-μ-calpain cleaves apoptosis inducing factor (AIF), a flavoprotein located within the mitochondrial intermembrane space, in liver mitocho...

journal_title：Biochemical and biophysical research communications

authors： Chen Q,Paillard M,Gomez L,Ross T,Hu Y,Xu A,Lesnefsky EJ

更新日期：2011-12-02 00:00:00

abstract：:FKBP59-HBI, a heat shock protein hsp90-binding immunophilin that was originally detected in heterooligomer forms of steroid receptors, is retained on Calmodulin (CAM)-Sepharose 4B in the presence of 2 mM Ca2+ and is eluted by EGTA, demonstrating a specific p59-CAM interaction. The p59 amino acid sequence reveals the p...

journal_title：Biochemical and biophysical research communications

authors： Massol N,Lebeau MC,Renoir JM,Faber LE,Baulieu EE

更新日期：1992-09-30 00:00:00

abstract：:Corneal epithelial stem cells are believed to reside in the basal layer of the limbal epithelium, but no definitive cell surface markers have been identified. For keratinocytes, stem/progenitor cells are known to be enriched by cell surface markers, integrin alpha(6) and CD71, as a minor subpopulation which shows high...

journal_title：Biochemical and biophysical research communications

authors： Hayashi R,Yamato M,Saito T,Oshima T,Okano T,Tano Y,Nishida K

更新日期：2008-03-07 00:00:00

abstract：:Hepatocytes from human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are expected to be a useful source for cell transplantation. However, relatively low efficiency and repeatability of hepatic differentiation of human BM-MSCs remains an obstacle for clinical translation. Hepatocyte nuclear factor 4 alpha (HNF...

journal_title：Biochemical and biophysical research communications

authors： Hu X,Xie P,Li W,Li Z,Shan H

更新日期：2016-09-16 00:00:00

abstract：:We have established that differing effects of alpha1-adrenergic receptor (AR) subtypes on cell proliferation are due to differential coupling to the Gs/cAMP pathway; thus, both alpha1A- and alpha1B-ARs couple to Gs, while alpha1D-AR does not. To identify the region responsible for this difference in subtype-specific G...

journal_title：Biochemical and biophysical research communications

authors： Shinoura H,Shibata K,Hirasawa A,Tanoue A,Hashimoto K,Tsujimoto G

更新日期：2002-11-22 00:00:00

abstract：:We have identified a novel reaction in which acetaldehyde promotes rat hepatic cytosolic catalysis of O2 consumption coupled with glutathione oxidation without apparent release of activated forms of O2. Acetaldehyde is not consumed in the reaction. The reaction (O2 consumption or oxidized glutathione production) is sa...

journal_title：Biochemical and biophysical research communications

authors： Lyon I,Kaplowitz N

更新日期：1985-06-28 00:00:00

abstract：:Endotoxemia is characterized by multiple dysfunctions of the micro-vascular endothelium. Of these, vascular leakage is an initial event that aggravates vascular dysfunction can lead to systemic vascular collapse and organ failure. Thus, prevention of vascular leakage may ameliorate endotoxin-induced dysfunctions of bl...

journal_title：Biochemical and biophysical research communications

authors： Hwang JA,Lee EH,Lee SD,Park JB,Jeon BH,Cho CH

更新日期：2009-04-17 00:00:00

abstract：:18,54-SF cells, which secrete rat insulin-like growth factor II (rIGF-II), have abundant type 2 IGF receptors. We have purified the type 2 receptor from these cells by solubilization of crude membranes in Triton X-100, followed by chromatography on agarose-immobilized rIGF-II. A partially purified receptor preparation...

journal_title：Biochemical and biophysical research communications

authors： Rosenfeld RG,Hodges D,Pham H,Lee PD,Powell DR

更新日期：1986-07-16 00:00:00

abstract：:Through the proteomic analysis using 2-dimensional electrophoresis, the nicotine addiction-associated proteins were extensively screened in the striatum of rat brains. The nicotine addiction was developed by repeated nicotine injection (0.4mg/kg s.c.), twice daily for 7 days, followed by one challenge injection after ...

journal_title：Biochemical and biophysical research communications

authors： Yeom M,Shim I,Lee HJ,Hahm DH

更新日期：2005-01-14 00:00:00

abstract：:The ocr protein of bacteriophage T7 is a structural and electrostatic mimic of approximately 24 base pairs of double-stranded B-form DNA. As such, it inhibits all Type I restriction and modification (R/M) enzymes by blocking their DNA binding grooves and inactivates them. This allows the infection of the bacterial cel...

journal_title：Biochemical and biophysical research communications

authors： Stephanou AS,Roberts GA,Tock MR,Pritchard EH,Turkington R,Nutley M,Cooper A,Dryden DT

更新日期：2009-01-02 00:00:00

abstract：:A single class of saturable, specific binding sites for the circulating form of atrial natriuretic peptides, ANP(99-126), was identified in rat thymus and spleen and in isolated thymocytes and spleen cells using quantitative autoradiographic techniques. In the thymus, the relative potency of ANP analogs to inhibit [12...

journal_title：Biochemical and biophysical research communications

authors： Kurihara M,Katamine S,Saavedra JM

更新日期：1987-06-15 00:00:00

abstract：:Reversal of the plasma membrane Na(+)/Ca(2+) exchanger (NCX) has been shown to mediate Ca(2+) influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demonstrated by our la...

journal_title：Biochemical and biophysical research communications

authors： Syyong HT,Poburko D,Fameli N,van Breemen C

更新日期：2007-06-15 00:00:00

abstract：:Specific receptors for [3H]-15 HETE have been identified on GH3 cells, a cloned strain of rat pituitary cells. With incremental inputs of radioligand and a constant cell number, specific [3H]-15 HETE binding reached a plateau indicative of saturable binding sites. Ligand analysis of the Scatchard plot demonstrated a s...

journal_title：Biochemical and biophysical research communications

authors： Rabier M,Crastes de Paulet A,Damon M

更新日期：1988-05-16 00:00:00

abstract：:5-Aminosalicylic acid (5-ASA) is conventionally used as a first line drug for inflammatory bowel disease (IBD). Because 5-ASA is well absorbed in the small intestine, very high dose of 5-ASA is required to deliver it to the large intestine which is a target site. Interestingly, 5-ASA is reported to be transported into...

journal_title：Biochemical and biophysical research communications

authors： Yuri T,Kono Y,Fujita T

更新日期：2020-04-09 00:00:00

abstract：:Studies were undertaken to determine the immunochemical relationship between constitutive trout cytochrome P450s and mammalian cytochrome P450IIIA enzymes. Polyclonal antibodies (IgG) generated against trout P450 LMC5 reacted strongly with P450IIIA1 in dexamethasone-induced rat liver microsomes and with P450IIIA4 in h...

journal_title：Biochemical and biophysical research communications

authors： Miranda CL,Wang JL,Henderson MC,Zhao X,Guengerich FP,Buhler DR

更新日期：1991-04-30 00:00:00

abstract：:The nucleotide sequence of the promoter region of the melibiose operon of E. coli was determined. Consensus sequences for the -35 region, the Pribnow box and the binding site for cyclic AMP receptor protein were found in this region. The possible secondary structure of this DNA region was very similar to that of the p...

journal_title：Biochemical and biophysical research communications

authors： Shimamoto T,Yazyu H,Futai M,Tsuchiya T

更新日期：1984-05-31 00:00:00