Abstract:
:Dopamine neurons in the ventral tegmental area (VTA) play an important role in the motivational systems underlying drug addiction, and recent work has suggested that they also release the excitatory neurotransmitter glutamate. To assess a physiological role for glutamate corelease, we disrupted the expression of vesicular glutamate transporter 2 selectively in dopamine neurons. The conditional knockout abolishes glutamate release from midbrain dopamine neurons in culture and severely reduces their excitatory synaptic output in mesoaccumbens slices. Baseline motor behavior is not affected, but stimulation of locomotor activity by cocaine is impaired, apparently through a selective reduction of dopamine stores in the projection of VTA neurons to ventral striatum. Glutamate co-entry promotes monoamine storage by increasing the pH gradient that drives vesicular monoamine transport. Remarkably, low concentrations of glutamate acidify synaptic vesicles more slowly but to a greater extent than equimolar Cl(-), indicating a distinct, presynaptic mechanism to regulate quantal size.
journal_name
Neuronjournal_title
Neuronauthors
Hnasko TS,Chuhma N,Zhang H,Goh GY,Sulzer D,Palmiter RD,Rayport S,Edwards RHdoi
10.1016/j.neuron.2010.02.012subject
Has Abstractpub_date
2010-03-11 00:00:00pages
643-56issue
5eissn
0896-6273issn
1097-4199pii
S0896-6273(10)00103-0journal_volume
65pub_type
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