Covalent dimerization of camelidae anti-human TNF-alpha single domain antibodies by the constant kappa light chain domain improves neutralizing activity.

abstract：:A novel deuterium ((2)H) NMR technique as developed for measuring the total number of deuterons exchanged by lyophilised protein samples following hydrogen-deuterium (H-D) exchange. Using this methodology differences in the H-D exchange behaviour of the proteolytic enzyme subtilisin Carlsberg hydrated either in air or...

journal_title：Biotechnology and bioengineering

authors： Hutcheon GA,Parker MC,Moore BD

更新日期：2000-11-05 00:00:00

abstract：:The microbial degradation of quinoline by Comamonas acidovorans was studied in a laboratory scale stirred tank reactor. In continuous culture experiments using quinoline as a sole source of carbon and nitrogen, it was shown by means of mass balances that quinoline was converted completely to biomass, carbon dioxide, a...

journal_title：Biotechnology and bioengineering

authors： Miethling R,Hecht V,Deckwer WD

更新日期：1993-08-20 00:00:00

abstract：:Pre-filtration using ion exchange membrane adsorbers can improve parvovirus filter throughput of monoclonal antibodies (mAbs). The membranes work by binding trace foulants, and although some antibody product also binds, yields > or =99% are easily achieved by overloading. Results show that foulant adsorption is depend...

journal_title：Biotechnology and bioengineering

authors： Brown A,Bechtel C,Bill J,Liu H,Liu J,McDonald D,Pai S,Radhamohan A,Renslow R,Thayer B,Yohe S,Dowd C

更新日期：2010-07-01 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...

journal_title：Biotechnology and bioengineering

authors： Satianegara G,Rogers PL,Rosche B

更新日期：2006-08-20 00:00:00

abstract：:We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell d...

journal_title：Biotechnology and bioengineering

authors： Echtenkamp PL,Wilson DB,Shuler ML

更新日期：2009-02-15 00:00:00

abstract：:A denitrifying consortium capable of transforming carbon tetrachloride (CCl(4)) was cultured from aquifer sediment from the U.S. Department of Energy's Hanford Site in southeastern Washington State. To understand the kinetics of the biological destruction of CCl(4) by these microbes, a set of experiments, the conditio...

journal_title：Biotechnology and bioengineering

authors： Petersen JN,Skeen RS,Amos KM,Hooker BS

更新日期：1994-03-15 00:00:00

abstract：:A new biomass productivity model was parameterized for Chlorella vulgaris using short-term (<30 min) oxygen productivities from algal microcosms exposed to 6 light intensities (20-420 W/m(2)) and 6 temperatures (5-42 °C). The model was then validated against experimental biomass productivities recorded in bench-scale ...

journal_title：Biotechnology and bioengineering

authors： Béchet Q,Chambonnière P,Shilton A,Guizard G,Guieysse B

更新日期：2015-05-01 00:00:00

abstract：:Modern large-scale agricultural practices that incorporate high density farming with subtherapeutic antibiotic dosing are considered a major contributor to the rise of antibiotic-resistant bacterial infections of humans with species of Salmonella being a leading agriculture-based bacterial infection. Microcin J25, a p...

journal_title：Biotechnology and bioengineering

authors： Ritter SC,Yang ML,Kaznessis YN,Hackel BJ

更新日期：2018-10-01 00:00:00

abstract：:A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...

journal_title：Biotechnology and bioengineering

authors： Agena SM,Pusey ML,Bogle ID

更新日期：1999-07-20 00:00:00

abstract：:Growth of microorganisms on substitutable substrate mixtures display diverse growth dynamics characterized by simultaneous or preferential uptake of carbon sources. This article shows that cybernetic modeling concepts which were successful in predicting diauxic growth patterns can be extended to describe simultaneous ...

journal_title：Biotechnology and bioengineering

authors： Ramakrishna R,Ramkrishna D,Konopka AE

更新日期：1996-10-05 00:00:00

abstract：:Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

journal_title：Biotechnology and bioengineering

authors： Davidson AJ,Ellis MJ,Chaudhuri JB

更新日期：2010-08-15 00:00:00

abstract：:The anaerobic digestion of a cellulose-enriched fraction of domestic refuse by means of rumen microorganisms in an "artificial rumen" digester was studied. Various combinations of solid and liquid retention times and loading rates were applied to establish optimum conditions for the acidogenic phase digestion of the r...

journal_title：Biotechnology and bioengineering

authors： Gijzen HJ,Zwart KB,Teunissen MJ,Vogels GD

更新日期：1988-09-05 00:00:00

abstract：:Chinese hamster ovary (CHO) cells in culture are known to consume large amounts of nutrients and divert most of them toward byproducts, some of which, including lactate and ammonia, are known to be toxic in nature. Glucose limitation strategies can successfully control lactate accumulation in fed-batch cultures yieldi...

journal_title：Biotechnology and bioengineering

authors： Mulukutla BC,Kale J,Kalomeris T,Jacobs M,Hiller GW

更新日期：2017-08-01 00:00:00

abstract：:Protein dynamic binding capacities on ion exchange resins are typically expected to decrease with increasing conductivity and decreasing protein charge. There are, however, conditions where capacity increases with increasing conductivity and decreasing protein charge. Capacity measurements on two different commercial ...

journal_title：Biotechnology and bioengineering

authors： Harinarayan C,Mueller J,Ljunglöf A,Fahrner R,Van Alstine J,van Reis R

更新日期：2006-12-05 00:00:00

abstract：:Anaerobic methanethiol (MT) degradation by mesophilic (30 degrees C) alkaliphilic (pH 10) communities was studied in a lab-scale Upflow Anaerobic Sludge Bed (UASB) reactor inoculated with a mixture of sediments from the Wadden Sea (The Netherlands), Soap Lake (Central Washington), and Russian soda lakes. MT degradatio...

journal_title：Biotechnology and bioengineering

authors： van Leerdam RC,Bonilla-Salinas M,de Bok FA,Bruning H,Lens PN,Stams AJ,Janssen AJ

更新日期：2008-11-01 00:00:00

abstract：:Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied in wastewater-fed M...

journal_title：Biotechnology and bioengineering

authors： Bourdakos N,Marsili E,Mahadevan R

更新日期：2014-04-01 00:00:00

abstract：:Membrane adsorbers may be a viable alternative to the packed-bed chromatography for clearance of virus, host cell proteins, DNA, and other trace impurities. However, incorporation of membrane adsorbers into manufacturing processes has been slow due to the significant cost associated with obtaining regulatory approval ...

journal_title：Biotechnology and bioengineering

authors： Weaver J,Husson SM,Murphy L,Wickramasinghe SR

更新日期：2013-02-01 00:00:00

abstract：:All bacteria release organic compounds called soluble microbial products (SMP) as a part of their normal metabolism. In photobioreactor (PBR) settings, SMP produced by cyanobacteria represent a major pool of carbon and electrons available to heterotrophic bacteria. Thus, SMP in PBRs are a major driver for the growth o...

journal_title：Biotechnology and bioengineering

authors： Zevin AS,Nam T,Rittmann B,Krajmalnik-Brown R

更新日期：2015-09-01 00:00:00

abstract：:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...

journal_title：Biotechnology and bioengineering

authors： Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA

更新日期：2015-09-01 00:00:00

abstract：:Purified rat liver phenylalanine hydroxylase [L-phenylalanine:tetrahydropteridine:oxygen oxidoreductase (4-hydroxylating), EC 1.14.16.1] was immobilized with activated thiol-Sepharose 4B via disulfide bond formation, which is expected to immobilize the enzyme in its activated form through the SH modification. This imm...

journal_title：Biotechnology and bioengineering

authors： Koizumi S,Takeuchi T,Umezawa H,Nagatsu T

更新日期：1986-04-01 00:00:00

abstract：:A transient, two-culture model simulating methane production from biomass has been developed. The simulator, based partially on the work by Andrews and McCarty, is capable of calculating the hydrolysis products of several common organic materials, accommodating various substrate feeding modes, and simulating the trans...

journal_title：Biotechnology and bioengineering

authors： Kleinstreuer C,Poweigha T

更新日期：1982-09-01 00:00:00

abstract：:Spores, although often considered metabolically inert, catalyze a variety of reactions. The use of spores instead of mycelium for bioconversions has several advantages. In this paper, we describe the difference in susceptibility of mycelium and spores against toxic substrates and products. A higher resistance of spore...

journal_title：Biotechnology and bioengineering

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2002-12-20 00:00:00

abstract：:During lignocellulosic ethanol fermentation, yeasts are exposed to various lignocellulose-derived inhibitors, which disrupt the efficiency of hexose and pentose co-fermentation. To understand the metabolic response of fermentation microbes to these inhibitors, a comparative metabolomic investigation was performed on a...

journal_title：Biotechnology and bioengineering

authors： Wang X,Jin M,Balan V,Jones AD,Li X,Li BZ,Dale BE,Yuan YJ

更新日期：2014-01-01 00:00:00

abstract：:The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...

journal_title：Biotechnology and bioengineering

authors： Junker B

更新日期：2001-07-05 00:00:00

abstract：:Hemicellulose residues can be hydrolyzed into a sugar syrup using dilute mineral acids. Although this syrup represents a potential feedstock for biofuel production, toxic compounds generated during hydrolysis limit microbial metabolism. Escherichia coli LY01, an ethanologenic biocatalyst engineered to ferment the mixe...

journal_title：Biotechnology and bioengineering

authors： Zaldivar J,Ingram LO

更新日期：1999-01-01 00:00:00

abstract：:We report the first direct images of the hydration of protein powders. Using an environmental scanning electron microscope (ESEM) we have taken a series of micrographs of a region of the enzyme (subtilisin) power whilst hydrating the sample. In addition, the sample has been viewed during exposure to toluene vapors. Th...

journal_title：Biotechnology and bioengineering

authors： Roziewski K,Russell AJ

更新日期：1992-05-01 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without sel...

journal_title：Biotechnology and bioengineering

authors： González Vara A,Rossi M,Altomare L,Eikmanns B,Matteuzzi D

更新日期：2003-10-20 00:00:00

abstract：:In view of the biochemical reaction catalyzed by enzyme powder suspended in a water-insoluble organic solvent, an equation was derived to estimate the amount of water bound to the enzyme powder. With this equation, an apparent adsorption isotherm between free water (water freely dissolved in benzene) and bound water (...

journal_title：Biotechnology and bioengineering

authors： Yamane T,Kojima Y,Ichiryu T,Nagata M,Shimizu S

更新日期：1989-09-01 00:00:00