Abstract:
:The objective of this study was to determine if prolonged hyperoxia exposure would deplete antioxidants, resulting in excessive oxidative stress that would lead to oxidation of pulmonary surfactant and contribute to lung dysfunction. Rats were exposed to either hyperoxic (> 95% O(2)) or normoxic (21% O(2)) oxygen concentrations for 48 or 60 hours. Pulmonary compliance, inflammatory cells, and total protein levels were measured as indicators of lung injury. Bronchoalveolar lavage (BAL) samples were analyzed for surfactant composition, antioxidant content, and markers of oxidative stress. Antioxidants were also measured in lung tissue and plasma samples. Hyperoxia exposure for 60 hours resulted in increased protein and inflammatory cells in BAL, and lower pulmonary compliance, compared to all other groups. Total surfactant and surfactant large aggregates were increased following 48 hours of hyperoxia exposure, with a further increase following 60 hours. Animals exposed to 60 hours of hyperoxia also demonstrated lower ascorbate levels in lung tissue, increased lipid peroxides in BAL, and increased oxidation of phosphatidylglycerol species in surfactant. This study demonstrates that the balance of oxidant/antioxidant components is disrupted within the lung during periods of hyperoxia, and that although surfactant lipids may be susceptible to oxidative damage, they do not likely represent a major mechanism for the lung dysfunction observed.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Pace PW,Yao LJ,Wilson JX,Possmayer F,Veldhuizen RA,Lewis JFdoi
10.1080/01902140902745166subject
Has Abstractpub_date
2009-06-01 00:00:00pages
380-98issue
5eissn
0190-2148issn
1521-0499pii
10.1080/01902140902745166journal_volume
35pub_type
杂志文章abstract:PURPOSE OF THE STUDY:Acute respiratory distress syndrome (ARDS) represents a major cause of mortality in intensive care patients. Activation of peroxisome proliferator-activated receptor-α (PPAR-α) by fibrates, such as WY-14643 (WY), has been described to beneficially influence inflammation and experimental lung injury...
journal_title:Experimental lung research
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abstract::Nuclear factor kappa B (NF-kappa B) is a critical transcription factor for the production of many inflammatory cytokines. It is activated in the airway epithelium of human asthmatics and in mice after allergic stimulation. To examine the role of NF-kappa B activation in allergic inflammation, the authors generated tra...
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abstract::Lung cancer is the leading cause of death of both men and women across the world. Overexpression and activating mutations of the epidermal growth factor receptor-1 (EGFR1) are frequently observed and associated with poor prognosis. To inhibit the function of EGFR1, multiple antibodies and small-molecule tyrosine kinas...
journal_title:Experimental lung research
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abstract::Inflammation plays a key role in lung injury and in the pathogenesis of asthma. Two murine models of allergic airway inflammation-sensitization and challenge to ovalbumin (OVA) and intratracheal exposure to interleukin-13 (IL13)-were used to evaluate the expression of poly(ADP-ribose) polymerase-1 (PARP-1) in allergic...
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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doi:10.3109/01902148.2011.633306
更新日期:2012-02-01 00:00:00
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journal_title:Experimental lung research
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更新日期:1998-05-01 00:00:00
abstract::Wild-type K-ras is tumor suppressive in mouse lung, but mutant K-ras is actively oncogenic. Thus, the mutant protein must acquire new, dominant protumorigenic properties. Generation of reactive oxygen species could be one such property. The authors demonstrate increased peroxides in lung epithelial cells (E10)-transfe...
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abstract::The aim of the study was to identify the effects on the lung after subchronic glass fiber intratracheal instillation study on rats. We evaluated the toxicological effects on the lung: persistent inflammatory reaction, cell proliferation, and pulmonary fibrosis on histopathological examination. We performed a glass fib...
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doi:10.3109/01902148.2013.860568
更新日期:2014-03-01 00:00:00
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更新日期:1992-09-01 00:00:00
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journal_title:Experimental lung research
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abstract::It has been suggested that increased recruitment of phagocytes and subsequent clearance of particles may follow instillation of a leukocyte chemoattractant to lungs containing silica. The present study quantitated serially the silica content in alveolar spaces, in lung tissue and in hilar lymph nodes of mice that rece...
journal_title:Experimental lung research
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doi:10.3109/01902149409064384
更新日期:1994-05-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148409087917
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更新日期:2002-06-01 00:00:00
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更新日期:2015-06-01 00:00:00
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更新日期:2014-02-01 00:00:00
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