Abstract:
:Biochemical characterization of human respiratory mucus has generally utilized expectorated specimens. In order to exclude extraneous contaminants in the analysis of airway glycoproteins, human airways were cultured and the mucous glycoprotein released into the supernatant analyzed. By incorporating 3H-labeled glucosamine or 14C-threonine into the media, the airways biosynthetically labeled the mucous glycoproteins (MGP), facilitating their analysis. The MGP chromatograph by gel filtration on Sepharose 2B in two fractions: one excluded from the column and one that enters the column. However, employing a gel filtration column with the ability to fractionate larger molecules, Sephacryl S-1000, it was found that MGP fractionate over a large range in molecular sizes and do not segregate into distinct fractions. The diffuse, broad peak of MGP fractionation on Sephacryl S-1000 is not affected by reduction and alkylation or by chromatography in 1 M NaCl. The fractionated MGP from Sepharose 2B were divided into larger and smaller molecular species, and their charge characteristics were determined by DEAE chromatography and preparative isoelectric focussing. MGP exhibit strong acidic charge characteristics that are uniform, as reflected in elution from DEAE and a single, sharp isoelectric focussing point. Enzymatic cleavage of the oligosaccharide side chains from MGP liberates more than 70% of the radiolabeled side chains. The side chains enzymatically cleaved from the larger and smaller molecular species of MGP are similar in size. Highly purified MGP were found to be 73% carbohydrate and 27% protein. Thus, human airways release a family of MGP that express marked heterogeneity in size but a uniform, strong acid charge and include side chains of similar size.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Shelhamer JH,Marom Z,Logun C,Kaliner Mdoi
10.3109/01902148409069675subject
Has Abstractpub_date
1984-01-01 00:00:00pages
149-62issue
2eissn
0190-2148issn
1521-0499journal_volume
7pub_type
杂志文章abstract::In both ovalbumin-sensitized and ovalbumin-challenged guinea pigs, the phospholipid content of bronchoalveolar lavage fluid is decreased with respect to that of controls. In the sensitized guinea pig, the activity of lung membrane phospholipase is increased and the phospholipid content of lung membranes is decreased. ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149009087876
更新日期:1990-03-01 00:00:00
abstract::The role of type II epithelial cell proliferation in repair of diffuse alveolar epithelial injury was examined using two pneumotoxicants, 3-methylindole (3-MI) and monocrotaline (MCT). It was hypothesized that if MCT inhibits type II epithelial cell mitosis, then pulmonary fibrosis would result after diffuse 3-MI-indu...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270376
更新日期:1999-03-01 00:00:00
abstract::We hypothesized that replication-deficient adenovirus (Ad), when complexed with plasmid DNA (pl) and cationic liposomes (L), would enhance liposome-mediated gene transfer in cultured human airway epithelial cells. Pl/L/Ad complexes were formed using charge-charge interactions. A gel electrophoresis retardation assay s...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021400269862
更新日期:2000-04-01 00:00:00
abstract::In vitro responses of potential target cell types to air pollutants under physiological conditions may be useful in understanding the health effects of air pollution exposure. The study evaluated responses of human primary airway epithelial cells to diesel exhaust (DE). Cultures of cells from 3 donors, differentiated ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601113088
更新日期:2007-01-01 00:00:00
abstract:AIM OF THE STUDY:Nucleic acid-based therapies have the potential to provide clinically meaningful benefit across a wide spectrum of lung disease. However, in vivo delivery remains a challenge. Here we examined the feasibility of using electrospray to deliver nucleic acids to both porcine tracheal tissue sections and wh...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1563924
更新日期:2018-01-01 00:00:00
abstract::Wild-type K-ras is tumor suppressive in mouse lung, but mutant K-ras is actively oncogenic. Thus, the mutant protein must acquire new, dominant protumorigenic properties. Generation of reactive oxygen species could be one such property. The authors demonstrate increased peroxides in lung epithelial cells (E10)-transfe...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.1080/01902140490495048
更新日期:2005-01-01 00:00:00
abstract::Sex-related differences may be present during fetal lung growth and at the onset of surfactant synthesis. In this study we investigated the role of dihydrotestosterone (DHT) and estrogen (EST) on cell division and on labeled palmitate incorporation into disaturated phosphatidylcholine (DSPC) at various times of gestat...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087851
更新日期:1989-03-01 00:00:00
abstract::We determined optimum conditions for delivering DNA to transformed human bronchial epithelial cells expressing wild-type (BEAS) or abnormal (2CF) cystic fibrosis transmembrane conductance regulator (CFTR) using cationic liposomes (Lipofectin, [N-(N,N-dimethylaminoethane)carbamyl] cholesterol[DC-Chol]/dioleoylphosphati...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270259
更新日期:1999-04-01 00:00:00
abstract:PURPOSE OF THE STUDY:Acute respiratory distress syndrome (ARDS) represents a major cause of mortality in intensive care patients. Activation of peroxisome proliferator-activated receptor-α (PPAR-α) by fibrates, such as WY-14643 (WY), has been described to beneficially influence inflammation and experimental lung injury...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1046200
更新日期:2015-01-01 00:00:00
abstract::Thrombin activates protease-activated receptor (PAR)-1 and induces a myofibroblast phenotype in normal lung fibroblasts. The origins of myofibroblasts are resident fibroblasts, fibrocytes, and epithelial-mesenchymal transition (EMT). We investigated the effects of thrombin, an important mediator of interstitial lung f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.820809
更新日期:2013-10-01 00:00:00
abstract::The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701703333
更新日期:2007-10-01 00:00:00
abstract::Both hyperoxia-induced proapoptotic sensitization of alveolar type II cells (TII cells) and high-stretch mechanical ventilation induced pulmonary inflammation are tumor necrosis factor alpha (TNFalpha) mediated. Therefore, binding of free TNFalpha should protect from TNFalpha-mediated acute lung injury and ameliorate ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902887430
更新日期:2009-11-01 00:00:00
abstract::The effects of Zizyphi fructus, a major constituent of Chinese anti-asthmatic herbal medicine, on ciliary motility and nitric oxide (NO) generation in canine cultured tracheal epithelium were studied by the microphoto-oscillation method and the specific amperometric method, respectively. Addition of Zizyphi fructus at...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149609031774
更新日期:1996-05-01 00:00:00
abstract::Alveolar cells were evaluated in BALB/c mice exposed to smoke from 9 cigarettes per day. The mice were sacrificed at 1, 5, and 10 days and examined by lung morphometry and immunohistochemical staining of alveolar macrophages for tumor necrosis factor (TNF)-alpha, matrix metalloproteinase (MMP)-12, and tissue inhibitor...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140500324828
更新日期:2005-10-01 00:00:00
abstract::Previously, this laboratory has shown that intratracheally administered hyaluronic acid (HA) significantly reduces air-space enlargement in a hamster model of emphysema induced with pancreatic elastase. Whereas HA was given immediately following elastase in those initial studies, the current investigation determined t...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149709087369
更新日期:1997-05-01 00:00:00
abstract::It is evident that much remains to be learned about the nasal passages and their responses to toxic materials. For the nose of both laboratory animals and humans, information is needed in the areas of anatomy, physiology, biochemistry, neurobiology, physiopathology, and oncology. This article briefly discussed toxic a...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149109064322
更新日期:1991-09-01 00:00:00
abstract::Excess production of neutrophil elastase contributes to the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the role of neutrophil elastase in the repair process following ALI/ARDS is not well understood. The objective of this study was to evaluate the effect of neutrop...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2011.633306
更新日期:2012-02-01 00:00:00
abstract::A tandem repeat region in the second intron of the K-ras gene has been reported to be a possible regulatory site for transcription. In this study, a second protein-binding site was identified and characterized. It lies downstream (nucleotides 463 to 509) of the tandem repeat region. A T--> C base variation at nucleoti...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/0190214049049552
更新日期:2005-03-01 00:00:00
abstract::Lung cancer, specifically non-small cell lung cancer (NSCLC), is a leading cause of mortality worldwide. In China, a dramatic increase in the incidence of NSCLC is expected in the next 20 years (Molina et al. Mayo Clin Proc. 2008;83:584–594). Mutated epidermal growth factor receptor (EGFR) status is a known predictor ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.482176
更新日期:2010-11-01 00:00:00
abstract::The objective of this study was to assess the radioprotective effects of amifostine in the rat model of radiation-induced lung injury using fractionated doses of radiation, to determine whether amifostine given before irradiation protects tumor from radiation cytotoxicity, and to determine whether changes in plasma le...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290096791
更新日期:2002-10-01 00:00:00
abstract::A cast of human tracheobronchial airways, which is complete to airways less than 0.1 cm in diameter, was employed for experimental studies of gas convective transport by high-frequency ventilation (HFV). The cast was ventilated with gases of different kinematic viscosity (He, air, and SF6) and tidal volumes of 10-60 m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
abstract::Aerosol bolus dispersion was measured in the first branching generations of a replicate hollow cast. The cast was made from a solid cast of a beagle-dog tracheobronchial tree. The dispersion was measured after penetration of boluses into different volumetric depths, with different particle sizes and flow rates into th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031756
更新日期:1995-07-01 00:00:00
abstract::The Ca(2+)-mobilizing actions of adenosine 5'-triphosphate (ATP), bradykinin, and histamine were compared in phenotypically distinct human nasal epithelial (HNE) cell types and as a function of time in cell culture. Single-cell measurements of intracellular free Ca2+ (Ca2+i, Fura-2 fluorescence) were recorded in cilia...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270196
更新日期:1999-06-01 00:00:00
abstract::Lung injury and inflammation are associated with exposure to various types of particulate air pollutants. The present study was used to determine whether metalloproteinases (MMPs) are secreted after instilling dust samples into the lung, and to relate levels of specific MMPs to different fractions of the ambient air p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140303753
更新日期:2003-09-01 00:00:00
abstract::Activity for nonspecific esterases has been demonstrated histochemically in fetal rabbit lungs using alpha-naphthyl acetate as substrate. Commencing with a homogeneous distribution in pseudoglandular lungs, activity gradually heightens in the epithelium, progresses centrifugally along the bronchial tree, and reaches f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209069657
更新日期:1982-11-01 00:00:00
abstract:AIMS:As a transcription factor of the nuclear receptor superfamily, neuron-derived orphan receptor 1 (NOR1) is induced rapidly in response to various extracellular stimuli. But, it is still unclear its role in pulmonary artery smooth muscle cells proliferation. MATERIALS AND METHODS:Human PASMCs were cultured in vitro...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2014.993776
更新日期:2015-05-01 00:00:00
abstract::A small subpopulation of pulmonary epithelial cells (PE) proliferates in low-density primary culture of alveolar type II cells and forms colonies of cells that could be passaged for several generations and that in some respects maintain a differentiated phenotype of the alveolar type II cells. At this time it is not k...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309031717
更新日期:1993-03-01 00:00:00
abstract::The separate effects of positive end-expiratory pressure (PEEP) and intravascular volume administration on the histopathologic lung injury were not investigated in experimental lung injury previously. The authors hypothesized that high PEEP and a restrictive volume therapy would yield the best oxygenation and the leas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.514023
更新日期:2011-02-01 00:00:00
abstract::Expression of microRNAs (miRNAs) is characteristically altered in cancer, and they may play a role in cancer development and progression. The authors performed microarray and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses to determine the miRNA expression profile of primary sm...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902822312
更新日期:2009-10-01 00:00:00
abstract::This study describes the morphology of the adaptive response of the pulmonary parenchyma after the resection of lung tissue. Rats aged 23 days were subjected to the bilobectomy of the right upper and middle lobes representing 25% of the total lung volume. On the postoperative Days 1, 4, 6, 9, 12, 18, and 30, the lungs...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209039246
更新日期:1982-12-01 00:00:00