Abstract:
:Lung injury and inflammation are associated with exposure to various types of particulate air pollutants. The present study was used to determine whether metalloproteinases (MMPs) are secreted after instilling dust samples into the lung, and to relate levels of specific MMPs to different fractions of the ambient air particle sample EHC-93. Rats received an intratracheal injection of 5 mg dust samples in 0.5 ml water and were killed at intervals from 4 hours to 28 days later particle samples were EHC-93 whole dust, and the insoluble, leached, and soluble fractions of the same dust. Samples prepared from EHC-2K dust were also used, as were solutions of zinc and copper chloride. All samples induced inflammation as measured by increased inflammatory cells in bronchoalveolar lavage (BAL) fluid; the highest levels were found 1 to 3 days after instilling the whole dust. This dust also induced production of MMP-2 and MMP-9 as shown in zymograms. The leached dust induced predominantly MMP-9, which was maximal at 4 hours and 1 day. In contrast, the soluble fraction induced almost exclusive 4 MMP-2, also maximal at 4 hours and 1 day; this enzyme was also produced in response to soluble zinc, the most prevalent soluble metal in the EHC samples. The results demonstrate the rapid production and secretion of MMPs in the lung after particle deposition. A differential pattern of MMP production is seen with MMP-9, likely from inflammatory cells, being produced in response to the insoluble particles, and MMP-2, likey from epithelial cells, being produced in response to the water-soluble fraction of the atmospheric dust.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Adamson IY,Vincent R,Bakowska Jdoi
10.1080/01902140303753subject
Has Abstractpub_date
2003-09-01 00:00:00pages
375-88issue
6eissn
0190-2148issn
1521-0499pii
6P0535GQMDBG0B88journal_volume
29pub_type
杂志文章abstract::The objective of this study was to determine if prolonged hyperoxia exposure would deplete antioxidants, resulting in excessive oxidative stress that would lead to oxidation of pulmonary surfactant and contribute to lung dysfunction. Rats were exposed to either hyperoxic (> 95% O(2)) or normoxic (21% O(2)) oxygen conc...
journal_title:Experimental lung research
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doi:10.1080/01902140902745166
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journal_title:Experimental lung research
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087861
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abstract::It has been suggested that increased recruitment of phagocytes and subsequent clearance of particles may follow instillation of a leukocyte chemoattractant to lungs containing silica. The present study quantitated serially the silica content in alveolar spaces, in lung tissue and in hilar lymph nodes of mice that rece...
journal_title:Experimental lung research
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更新日期:1994-05-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:
更新日期:2005-09-01 00:00:00
abstract::We quantified in vivo pulmonary vascular remodeling in a large animal model of pulmonary hypertension (PH). In group PH (n = 6), 3 mg/kg dehydromonocrotaline (DHMC) was administered to 12-week-old beagles via a right atrial injection. Eight weeks after DHMC in group PH, pulmonary artery pressure increased significantl...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401459734
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journal_title:Experimental lung research
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
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journal_title:Experimental lung research
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2019.1631906
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087863
更新日期:1989-05-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148109052313
更新日期:1981-08-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2016.1207727
更新日期:2016-08-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.482176
更新日期:2010-11-01 00:00:00
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doi:10.1080/01902148.2018.1451574
更新日期:2018-03-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809087842
更新日期:1988-01-01 00:00:00
abstract::Previously, the authors have shown that tracheal occlusion (TO) partially reverses the onset of congenital diaphragmatic hernia (CDH)-induced pulmonary hypertension (PH) and abnormal pulmonary vascular development whereas release of the occlusion (TR) abolishes these clinical benefits. As a consequence of their mitoge...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021490927079
更新日期:2005-05-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
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journal_title:Experimental lung research
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doi:10.3109/01902148.2012.708385
更新日期:2012-09-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2017-03-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087899
更新日期:1984-01-01 00:00:00
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journal_title:Experimental lung research
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doi:10.1080/01902140150216819
更新日期:2000-12-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902149509050838
更新日期:1995-09-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902148009065460
更新日期:1980-08-01 00:00:00
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更新日期:2020-01-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902149109064417
更新日期:1991-03-01 00:00:00
abstract::Purpose/Aim of Study: Studies of pulmonary fibrosis (PF) have resulted in DNA damage, inflammatory response, and cellular senescence being widely hypothesized to play a role in the progression of the disease. Utilizing these aforementioned terms, genomics databases were interrogated along with the term, "pulmonary fib...
journal_title:Experimental lung research
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更新日期:2017-04-01 00:00:00