Abstract:
:Intratracheal bleomycin induces pulmonary fibrosis in experimental animals, but the mechanisms involved are poorly understood. Since altered levels of fatty acid metabolites are associated with bleomycin-induced lung injury, we examined the effects of a change in dietary fat on bleomycin-induced fibrosis. Previously we have shown that an essential fatty acid-deficient diet can reduce the severity of bleomycin-induced pulmonary fibrosis. The present study examined the effect of replacement of usual dietary fat with menhaden oil, rich in eicosapentaenoic acid, on the development of pulmonary fibrosis. Weanling rats were raised on a standard laboratory diet or a diet consisting of a fat-free powder to which was added 25% (w/w) of menhaden oil. After 8 weeks of feeding, the animals received either 1.5 units of bleomycin or an equivalent volume of saline intratracheally. In animals receiving the laboratory diet, bleomycin treatment produced a 44% increase in total lung protein content when compared to saline-treated controls (p less than 0.001) and a 77% increase in total lung hydroxyproline content (p less than 0.01). In contrast, bleomycin-treated animals receiving the menhaden oil diet had only small increases, which did not reach statistical significance, in protein and hydroxyproline content in the lung. Bronchoalveolar lavage cellularity did not differ among the treatment groups, but the percentage of lavage macrophages was slightly diminished in bleomycin-treated animals receiving the laboratory diet. Cellular differentials of lavage fluid did not differ significantly between bleomycin- and saline-treated animals receiving the menhaden oil diet. Bleomycin-induced histologic changes, quantitated by morphometric analysis, were significantly reduced with the menhaden oil diet. We conclude that a diet rich in eicosapentaenoic acid can significantly ameliorate bleomycin-induced pulmonary fibrosis, possibly via alterations in eicosanoid metabolism.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Kennedy JI Jr,Chandler DB,Fulmer JD,Wert MB,Grizzle WEdoi
10.3109/01902148909087861subject
Has Abstractpub_date
1989-03-01 00:00:00pages
315-29issue
2eissn
0190-2148issn
1521-0499journal_volume
15pub_type
杂志文章abstract:PURPOSE/AIM:Intravitreal bevacizumab (Avastin) is an irreversible vascular endothelial growth factor (VEGF) inhibitor used to treat severe retinopathy of prematurity (ROP) in extremely low gestational age neonates (ELGANs). ELGANs who are at the highest risk for developing severe ROP often experience brief intermittent...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1866712
更新日期:2020-12-30 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is characterized by systemic and local chronic inflammation and oxidative stress. The sources of the increased oxidative stress in COPD patients derive from the increased burden of inhaled oxidants such as cigarette smoke and other forms of particulate or gaseous air pollut...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148.2011.580416
更新日期:2011-08-01 00:00:00
abstract::The role of the Clara and type II cell in the development of pulmonary tumors in the A/J mouse and Fischer rat was investigated by determining the relationship of DNA methylation and repair in pulmonary cells to oncogene activation and by characterizing the morphology of pulmonary tumors induced by treatment with 4-(m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064417
更新日期:1991-03-01 00:00:00
abstract::We studied lung clearance of technetium-labeled diethylenetriamine pentaacetic acid [( 99mTc]DTPA), plasma and bronchoalveolar lavage fluid (BALF) concentrations of 6-keto-PGF1 alpha (stable metabolite of prostacyclin, prostaglandin I2, PGI2), TxB2 (stable metabolite of thromboxane A2, TxA2), and leukotriene B4 (LTB4)...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809087840
更新日期:1988-01-01 00:00:00
abstract::The separate effects of positive end-expiratory pressure (PEEP) and intravascular volume administration on the histopathologic lung injury were not investigated in experimental lung injury previously. The authors hypothesized that high PEEP and a restrictive volume therapy would yield the best oxygenation and the leas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.514023
更新日期:2011-02-01 00:00:00
abstract:INTRODUCTION:Nintedanib is a multi-tyrosine kinase receptor inhibitor recently approved for treatment of idiopathic pulmonary fibrosis. Although angiogenesis is a key process involved in airway structural changes in patients with bronchial asthma, the effect of nintedanib targeting the angiokinase pathway on airway inf...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1339141
更新日期:2017-01-01 00:00:00
abstract::The aim of this study was to determine influence of prenatal granulocyte-macrophage colony-stimulating factor (GM-CSF) administration on lung growth, maturation, and vascular endothelial growth factor (VEGF) expression. Twenty Wistar rats received sterile saline (1 mL) or recombinant human GM-CSF (50 micro g/kg) on da...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802341736
更新日期:2008-11-01 00:00:00
abstract::Survival of mice after Klebsiella pneumoniae infection and phagocytosis by alveolar macrophages (AMs), in the presence or absence of ozone (O(3)) exposure prior to infection, is sex dependent. The objective of this work was to study the role of gonadal hormones, 5α-dihydrotestosterone (DHT) and 17β-estradiol (E(2)), o...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2011.654045
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Hypoperfusion of the pulmonary vascular bed under the condition of congenital cardiac malformations may lead to progressive pulmonary vascular disease. To improve the mechanistic understanding of this disease, we examined the biochemical and morphological changes of the lung in a relevant animal model and pr...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.708385
更新日期:2012-09-01 00:00:00
abstract::Direct measurements of pleural fluid hydrostatic and colloid osmotic pressures after infusion of saline, bovine serum albumin, or silicone into the pleural space were coupled with ultrastructural morphometric analyses to assess the response of pleural mesothelial cells to hydrothorax. Increases of hydrostatic pressure...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309064347
更新日期:1993-05-01 00:00:00
abstract::The Ca(2+)-mobilizing actions of adenosine 5'-triphosphate (ATP), bradykinin, and histamine were compared in phenotypically distinct human nasal epithelial (HNE) cell types and as a function of time in cell culture. Single-cell measurements of intracellular free Ca2+ (Ca2+i, Fura-2 fluorescence) were recorded in cilia...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270196
更新日期:1999-06-01 00:00:00
abstract:PURPOSE OF THE STUDY:Acute respiratory distress syndrome (ARDS) represents a major cause of mortality in intensive care patients. Activation of peroxisome proliferator-activated receptor-α (PPAR-α) by fibrates, such as WY-14643 (WY), has been described to beneficially influence inflammation and experimental lung injury...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1046200
更新日期:2015-01-01 00:00:00
abstract::The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701539745
更新日期:2007-08-01 00:00:00
abstract::This report describes the protocol for the strain A mouse lung tumor bioassay and summarizes results on selected chemicals that have been tested for carcinogenicity in the assay. The assay is of 6 months duration and can distinguish 2-fold differences in carcinogenic potential of compounds from several chemical classe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064428
更新日期:1991-03-01 00:00:00
abstract::Administering liposome-encapsulated drugs by aerosols could be a feasible way of targeting drugs to the lung, specifically to pulmonary alveolar macrophages (AM). In the mouse model, we characterized uptake of carboxyfluorescein- (CF-) labeled liposomes by AM in vivo after acute inhalation of liposome aerosols, and th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309071077
更新日期:1993-01-01 00:00:00
abstract::Investigations on leukocyte populations in the lung have shown that lymphocytes are found in different anatomical compartments. Lymphocytes can be seen to a different extent in the lung interstitium, the epithelium and lamina propria of the bronchi, the bronchoalveolar space, and the marginal lung vascular bed. Previo...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149609070037
更新日期:1996-11-01 00:00:00
abstract::Amiodarone, a cardiac antiarrhythmic agent, has been associated with the development of interstitial pulmonary fibrosis in patients receiving prolonged therapy with the drug. To further assess the toxic effects of amiodarone on lung tissue, Syrian hamsters were given a single intratracheal insufflation of the agent an...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087891
更新日期:1984-01-01 00:00:00
abstract::The effect of glucocorticoids on the response of adenylate cyclase in fetal rat lung fibroblast and Type II epithelial cell cultures to beta-agonists and prostaglandin E2 (PGE2) was investigated. There was significant stimulation of cyclic AMP synthesis by adrenalin in both fibroblasts and Type II cells, which was pot...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087863
更新日期:1989-05-01 00:00:00
abstract::We quantified in vivo pulmonary vascular remodeling in a large animal model of pulmonary hypertension (PH). In group PH (n = 6), 3 mg/kg dehydromonocrotaline (DHMC) was administered to 12-week-old beagles via a right atrial injection. Eight weeks after DHMC in group PH, pulmonary artery pressure increased significantl...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401459734
更新日期:2001-01-01 00:00:00
abstract::A past study demonstrated that all-trans-retinoic acid (ATRA) treatment by intraperitoneal injection in a rat model of elastase-induced emphysema caused tissue regeneration as evidenced by a decrease in alveolar size and lung volume and an increase in alveolar number. We postulated that treatment with this retinoid by...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490463142
更新日期:2004-07-01 00:00:00
abstract::Urban air consists of a combination of environmental pollutants. Recent studies have suggested that normally innocuous doses of a particular pollutant may be rendered more toxic to the lung if primed by earlier events. Pulmonary inflammation has been observed in humans and in many animal species after endotoxin and oz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290092029
更新日期:2002-09-01 00:00:00
abstract::Leukotrienes (LTs) are potent vasoconstrictors in the pulmonary circulation. We investigated LTB4 and LTE4 metabolism by intrapulmonary arteries and veins of 2 to 9 days old lambs (n = 6). Paired vessels were incubated under baseline, and stimulated conditions. LTB4 and LTE4 were extracted from media, quantfied by enz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401750193601
更新日期:2001-06-01 00:00:00
abstract:PURPOSE OF THE STUDY:Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and trea...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1505976
更新日期:2018-08-01 00:00:00
abstract::Thrombin activates protease-activated receptor (PAR)-1 and induces a myofibroblast phenotype in normal lung fibroblasts. The origins of myofibroblasts are resident fibroblasts, fibrocytes, and epithelial-mesenchymal transition (EMT). We investigated the effects of thrombin, an important mediator of interstitial lung f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.820809
更新日期:2013-10-01 00:00:00
abstract::Retention in the extrathoracic airways, and clearance by nose blowing, of monodisperse indium-111-labeled polystyrene particles were followed for at least 2 days after inhalation by healthy volunteers. Nine volunteers inhaled 3-μm aerodynamic diameter particles while sitting at rest, whereas subgroups of 3 or 4 inhale...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.518301
更新日期:2011-03-01 00:00:00
abstract::Eprazinone therapy improves pulmonary function and arterial pO2 in patients with chronic bronchitis; however, the mechanism of action is unknown. The purpose of this study was to determine if eprazinone alters either lung surfactant levels in bronchoalveolar lavage fluid (BAL) of normal rats, or ion transport across c...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031693
更新日期:1992-05-01 00:00:00
abstract::Lung cancer is the leading cause of death of both men and women across the world. Overexpression and activating mutations of the epidermal growth factor receptor-1 (EGFR1) are frequently observed and associated with poor prognosis. To inhibit the function of EGFR1, multiple antibodies and small-molecule tyrosine kinas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.831960
更新日期:2013-11-01 00:00:00
abstract::Electrical stimulation of the cervical vagus nerve of rats is known to increase vascular permeability in the trachea. In the present study, we sought to further characterize this neurogenic inflammatory response by defining the relationship between the parameters of electrical stimulation of the vagus nerve and the ma...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809087842
更新日期:1988-01-01 00:00:00
abstract::Purpose: Mechanical ventilation (MV) is an essential life support tool for patients with acute respiratory distress syndrome (ARDS). However, MV for ARDS can result in ventilator-induced lung injury (VILI). This study aimed to assess whether alpha 1-antitrypsin (AAT) can reduce VILI in ARDS rats. Materials and Methods...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2019.1642968
更新日期:2019-09-01 00:00:00
abstract:AIM OF THE STUDY:Nucleic acid-based therapies have the potential to provide clinically meaningful benefit across a wide spectrum of lung disease. However, in vivo delivery remains a challenge. Here we examined the feasibility of using electrospray to deliver nucleic acids to both porcine tracheal tissue sections and wh...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1563924
更新日期:2018-01-01 00:00:00