Abstract:
:Thrombin activates protease-activated receptor (PAR)-1 and induces a myofibroblast phenotype in normal lung fibroblasts. The origins of myofibroblasts are resident fibroblasts, fibrocytes, and epithelial-mesenchymal transition (EMT). We investigated the effects of thrombin, an important mediator of interstitial lung fibrosis, on EMT in A549 human alveolar epithelial cells. We show that thrombin induced EMT and collagen I secretion through the activation of PAR-1, and PKC and ERK1/2 phosphorylation in A549 cells. These effects were largely prevented by a specific PAR-1 antagonist, short interfering RNA (siRNA) directed against PAR-1, or specific PKCα/β, δ, and ε inhibitors. These data indicated that interaction with thrombin and alveolar epithelial cells might directly contribute to the pathogenesis of pulmonary fibrosis through EMT. Targeting PAR-1 on the pulmonary epithelium or specific inhibitors to PKCα/β, δ, and ε might stop the fibrotic processes in human idiopathic pulmonary fibrosis by preventing thrombin-induced EMT.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Song JS,Kang CM,Park CK,Yoon HKdoi
10.3109/01902148.2013.820809subject
Has Abstractpub_date
2013-10-01 00:00:00pages
336-48issue
8eissn
0190-2148issn
1521-0499journal_volume
39pub_type
杂志文章abstract:AIM OF THE STUDY:Long-term exposure to cigarette smoke generates chronic obstructive pulmonary disease (COPD) in guinea pigs, but a comprehensive evaluation of changes in lung function, as assessed by barometric whole body plethysmography (WBP), is lacking. MATERIALS AND METHODS:Female guinea pigs were exposed to the ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1294632
更新日期:2017-03-01 00:00:00
abstract::Previously, this laboratory has shown that intratracheally administered hyaluronic acid (HA) significantly reduces air-space enlargement in a hamster model of emphysema induced with pancreatic elastase. Whereas HA was given immediately following elastase in those initial studies, the current investigation determined t...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149709087369
更新日期:1997-05-01 00:00:00
abstract::Nuclear factor kappa B (NF-kappa B) is a critical transcription factor for the production of many inflammatory cytokines. It is activated in the airway epithelium of human asthmatics and in mice after allergic stimulation. To examine the role of NF-kappa B activation in allergic inflammation, the authors generated tra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140903019710
更新日期:2009-12-01 00:00:00
abstract::It was hypothesized that IL-8, a neutrophil chemotaxin, contributes to the influx of neutrophils into the pleural cavity of patients with pleural effusions. Pleural fluids were collected from 57 patients including 13 with effusions due to congestive heart failure, 28 with pleural involvement by carcinoma, 5 with empye...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309031730
更新日期:1993-09-01 00:00:00
abstract::Cyclin D1 dysregulation and differential inactivation of p16INK4a and Rb have been observed in human lung cancer. In chemically induced mouse lung tumors, the p16INK4a gene is a target of inactivation, and Rb is reduced at the mRNA level (Northern blot) although similar at the protein level (Western blot) when compare...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809087383
更新日期:1998-07-01 00:00:00
abstract::Administering liposome-encapsulated drugs by aerosols could be a feasible way of targeting drugs to the lung, specifically to pulmonary alveolar macrophages (AM). In the mouse model, we characterized uptake of carboxyfluorescein- (CF-) labeled liposomes by AM in vivo after acute inhalation of liposome aerosols, and th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309071077
更新日期:1993-01-01 00:00:00
abstract::Tracheal occlusion (TO) performed surgically in utero near the end of gestation causes a rapid increase in the distension of future airspaces, resulting in accelerated lung development. The authors hypothesize that TO stimulates microvascular growth concomitant with a rapid increase in the expression of genes implicat...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701884331
更新日期:2008-03-01 00:00:00
abstract::The inhalation of inorganic dust can lead to the development of interstitial pulmonary fibrosis, characterized by the accumulation of fibroblasts and connective tissue matrix in the lung interstitium. The fibrosis causes alterations in the architecture of the lung parenchyma, resulting in abnormal gas exchange and hyp...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064332
更新日期:1991-11-01 00:00:00
abstract::Aberrant expression of key cell cycle regulatory genes is essential for the immortalization and transformation of cells in vitro. We examined 20 mouse lung epithelial cell lines (2 nontumorigenic, 5 nonmetastatic, and 13 metastatic) for mutations or alterations in the expression of key components of the Rb pathway (pR...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401300054064
更新日期:2001-04-01 00:00:00
abstract::Freeze-fracture was used to study the nonciliated epithelial (Clara) cell of rat bronchiole. En face views of the P face of the luminal cell membrane reveal particle-free bulging and circular depressions at the presumptive sites of the membrane fusion between the cell membrane and secretory granule membrane. Cross fra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052313
更新日期:1981-08-01 00:00:00
abstract::Two morphologic characteristics have been used to define the nonciliated bronchiolar epithelial cell: abundant agranular endoplasmic reticulum (AER) and membrane-bound avoid granules. To assess the ultrastructural homogeneity of this cell type in the lungs of large domestic mammals used as experimental models in pulmo...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148009069645
更新日期:1980-06-01 00:00:00
abstract::Activity for nonspecific esterases has been demonstrated histochemically in fetal rabbit lungs using alpha-naphthyl acetate as substrate. Commencing with a homogeneous distribution in pseudoglandular lungs, activity gradually heightens in the epithelium, progresses centrifugally along the bronchial tree, and reaches f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209069657
更新日期:1982-11-01 00:00:00
abstract:BACKGROUND:HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1451574
更新日期:2018-03-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701703333
更新日期:2007-10-01 00:00:00
abstract::Angiotensin-converting enzyme (ACE) generates angiotensin II and degrades bradykinin. This ectopeptidase acts as a marker in pathologic conditions, showing tissue and vascular damage. In order to evaluate serum and tissue ACE activity in bleomycin-associated lung damage, the authors studied morphological and enzymatic...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490266556
更新日期:2004-03-01 00:00:00
abstract::Retention in the extrathoracic airways, and clearance by nose blowing, of monodisperse indium-111-labeled polystyrene particles were followed for at least 2 days after inhalation by healthy volunteers. Nine volunteers inhaled 3-μm aerodynamic diameter particles while sitting at rest, whereas subgroups of 3 or 4 inhale...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.518301
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:This study aims to explore the effect of thymoquinone (TQ) on particulate matter 2.5 (PM2.5)-induced lung injury. METHODS:The PM2.5 sample was provided by Shenyang Environment Monitor Central Station. Lung injury was established by intratracheal instillation PM2.5 (7.5 mg/kg) followed by TQ treatment (20 an...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1801895
更新日期:2020-10-01 00:00:00
abstract::Alveolar cells were evaluated in BALB/c mice exposed to smoke from 9 cigarettes per day. The mice were sacrificed at 1, 5, and 10 days and examined by lung morphometry and immunohistochemical staining of alveolar macrophages for tumor necrosis factor (TNF)-alpha, matrix metalloproteinase (MMP)-12, and tissue inhibitor...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140500324828
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:Asthma is a chronic inflammatory disease of the airway that is mediated by T-helper 2(TH2) cells. Thymic stromal lymphopoietin (TSLP) can aggravate asthmatic lung inflammation by activating dendritic cells (DCs) to promote TH2 differentiation. TSLP promoter polymorphisms are associated with susceptibility to...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.714840
更新日期:2012-10-01 00:00:00
abstract::The developmental regulation of the rabbit surfactant-associated proteins, SP-A, SP-B, and SP-C, was investigated using Northern blot analysis. These proteins comprise approximately 10% by weight of pulmonary surfactant, a lipoprotein secreted by type II cells that reduces surface tension at the air-alveolar interface...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031707
更新日期:1992-11-01 00:00:00
abstract::Suboptimal intake of dietary zinc (Zn) is one of the most common nutritional problems worldwide. Previously, the authors have shown that zinc deficiency (ZD) produces oxidative and nitrosative stress in lung of male rats. The goal of this study is to test the effect of moderate ZD on insulin-like growth factor (IGF)-1...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140903061787
更新日期:2010-02-01 00:00:00
abstract::During lung development, the extracellular matrix undergoes dynamic remodeling. Matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs), are important enzymes that participate in regulating tissue remodeling. There is an abnormal balance of the synthesis and degradation of collagen...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140303783
更新日期:2003-06-01 00:00:00
abstract::Basement membranes (BM) are specialized extracellular matrices (ECM) which serve as complex interfaces between epithelia, peripheral nerves, or muscle cells and their surrounding tissue microenvironments. Their composition is known to include type IV collagen, laminin, entactin, heparan sulfate proteoglycan (HSPG, per...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149709074023
更新日期:1997-03-01 00:00:00
abstract::Neutrophilic inflammation is responsible for much of the tissue damage seen in many lung diseases. For resolution of inflammation to occur, neutrophils must die by apoptosis, allowing their recognition and removal by macrophages. The molecular events controlling this important regulatory step are poorly understood, in...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.1080/01902140701756778
更新日期:2007-12-01 00:00:00
abstract::The diabetic pregnancy is characterized by a delay in the process of fetal lung maturation. To determine whether high glucose conditions per se have an adverse impact on fetal lung phospholipid metabolism, 20-day fetal rat lung explants were cultured in F-12 medium (10 mM glucose) to which glucose was added to achieve...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309064361
更新日期:1993-11-01 00:00:00
abstract::Urban air consists of a combination of environmental pollutants. Recent studies have suggested that normally innocuous doses of a particular pollutant may be rendered more toxic to the lung if primed by earlier events. Pulmonary inflammation has been observed in humans and in many animal species after endotoxin and oz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290092029
更新日期:2002-09-01 00:00:00
abstract::Survival of mice after Klebsiella pneumoniae infection and phagocytosis by alveolar macrophages (AMs), in the presence or absence of ozone (O(3)) exposure prior to infection, is sex dependent. The objective of this work was to study the role of gonadal hormones, 5α-dihydrotestosterone (DHT) and 17β-estradiol (E(2)), o...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2011.654045
更新日期:2012-05-01 00:00:00
abstract::Biochemical characterization of human respiratory mucus has generally utilized expectorated specimens. In order to exclude extraneous contaminants in the analysis of airway glycoproteins, human airways were cultured and the mucous glycoprotein released into the supernatant analyzed. By incorporating 3H-labeled glucosa...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409069675
更新日期:1984-01-01 00:00:00
abstract::We set out to determine whether or not the "mucolytic" drug N-acetylcysteine would speed the reversal of cigarette smoke-induced secretory-cell hyperplasia to normal, similar to that found previously for two nonsteroidal antiinflammatory drugs. Cigarette smoke alone significantly (p less than 0.01) increased the numbe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809062848
更新日期:1988-01-01 00:00:00