Abstract:
:During lung development, the extracellular matrix undergoes dynamic remodeling. Matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs), are important enzymes that participate in regulating tissue remodeling. There is an abnormal balance of the synthesis and degradation of collagen and elastin in perinatal lung associated with congenital diaphragmatic hernia (CDH). This study was designed to (1) determine the expression and gelatinolytic activity patterns of MMPs 2 and 9 and TIMPs 1 and 2 in rat lungs during the perinatal period, and (2) to test the hypothesis that they are abnormal in nitrofen-induced CDH. Measurements were made using reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and zymography. The mRNA expression and activity of MMP 2 did not change significantly from embryonic day 16 to postnatal day 14. The most striking feature found was the rapid increase in the expression of MMP 9 soon after birth. Measurements were repeated on lung tissue isolated from embryonic rats with nitrofen-induced CDH. The expression and activity of MMPs and TIMPs were similar to control values and thus we conclude that these proteins appear not to be responsible for the altered extracellular matrix and morphological abnormalities noted in CDH lungs at birth.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Lemke RP,Zhang W,Balcerazak D,Kobayashi K,Schwingshackl A,Cheung PY,Dixon WT,Baracos VE,Greer JJdoi
10.1080/01902140303783subject
Has Abstractpub_date
2003-06-01 00:00:00pages
261-76issue
4eissn
0190-2148issn
1521-0499pii
8KMJL6T6X8WQUEXWjournal_volume
29pub_type
杂志文章abstract::Distinct barrier lesions and an apical-basal distribution of alveolar edema fluid in either moderate or high elevated pressure edema lungs have been found in previous studies. In the present study, quantitative measurements were obtained by using electron microscopy and morphometry of extravascular lung water and barr...
journal_title:Experimental lung research
pub_type: 杂志文章
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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doi:10.3109/01902148109052324
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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更新日期:1983-07-01 00:00:00
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journal_title:Experimental lung research
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更新日期:1989-12-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:
更新日期:2005-09-01 00:00:00
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更新日期:2009-12-01 00:00:00
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doi:10.3109/01902148809064185
更新日期:1988-01-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2012-05-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2008-11-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902149509031767
更新日期:1995-11-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809087842
更新日期:1988-01-01 00:00:00
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更新日期:2007-01-01 00:00:00
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journal_title:Experimental lung research
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