Abstract:
:Because high partial pressures of oxygen (O2) can cause peroxidative cleavage of membrane lipids, it is plausible to hypothesize that hyperoxia alters the physical state and composition of lipids in the membranes of pulmonary endothelial cells and that manipulation of the lipid profile may modify endothelial cell tolerance to hyperoxic injury. To test this, porcine pulmonary artery endothelial cells were exposed to 95% O2 at 1 atmosphere absolute (ATA) in the presence or absence of cis vaccenic acid (CVA), a monounsaturated fatty acid (C18:1#11). Plasma membrane fluidity was assessed by fluorescence spectroscopy, plasma membrane lipid composition was quantitated using thin layer and gas chromatography, and cytotoxicity was monitored by measuring release of lactate dehydrogenase (LDH). Hyperoxia caused peroxidation of membrane lipids and decreased fluidity in three distinct lipid domains within the plasma membrane. Incubation with CVA was associated with a reduction in the degree of unsaturation of the constituent fatty acids within all plasma membrane lipid subclasses except monoglycerides. CVA-treated cells were also more resistant to hyperoxic injury as judged by LDH release. These results support the hypothesis that cells with membranes in which the fatty acyl chains are more resistant to the disordering effects of high O2 tensions may be more resistant to O2 toxicity.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Block ERdoi
10.3109/01902148809064185subject
Has Abstractpub_date
1988-01-01 00:00:00pages
937-58eissn
0190-2148issn
1521-0499journal_volume
14 Supplpub_type
杂志文章abstract::Lung cancer, specifically non-small cell lung cancer (NSCLC), is a leading cause of mortality worldwide. In China, a dramatic increase in the incidence of NSCLC is expected in the next 20 years (Molina et al. Mayo Clin Proc. 2008;83:584–594). Mutated epidermal growth factor receptor (EGFR) status is a known predictor ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.482176
更新日期:2010-11-01 00:00:00
abstract:PURPOSE OF THE STUDY:Acute respiratory distress syndrome (ARDS) represents a major cause of mortality in intensive care patients. Activation of peroxisome proliferator-activated receptor-α (PPAR-α) by fibrates, such as WY-14643 (WY), has been described to beneficially influence inflammation and experimental lung injury...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1046200
更新日期:2015-01-01 00:00:00
abstract::The studies presented were designed to test the efficacy of farnesyltransferase inhibitors (FTIs) as potential chemopreventive compounds in the mouse lung tumor model, and in tumor cell lines. The compounds included manumycin, gliotoxin, dihydroepiandrosterone (DHEA), perillyl alcohol (POH), and FTI-276. Each of these...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140150216819
更新日期:2000-12-01 00:00:00
abstract::We compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. Judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. Lung volumes in both air- and saline-filled lungs and mean chord length (Lm) were incre...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069638
更新日期:1989-12-01 00:00:00
abstract::COPD remains a public health problem that has yet to be fully recognized by the public, the medical community or public health (government) officials, and much work remains to be done worldwide to reverse the increasing trends of this chronic lung disease. ...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:
更新日期:2005-09-01 00:00:00
abstract::Previous studies have indicated increased plasma levels of inducible nitric oxide synthase in lung. This study further examines the pulmonary expression of nitric oxide synthase (NOS) isoforms in an ovine model of acute lung injury induced by smoke inhalation and burn injury (S+B injury). Female range bred sheep (4 pe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802446832
更新日期:2009-03-01 00:00:00
abstract::The role of type II epithelial cell proliferation in repair of diffuse alveolar epithelial injury was examined using two pneumotoxicants, 3-methylindole (3-MI) and monocrotaline (MCT). It was hypothesized that if MCT inhibits type II epithelial cell mitosis, then pulmonary fibrosis would result after diffuse 3-MI-indu...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270376
更新日期:1999-03-01 00:00:00
abstract:AIM OF THE STUDY:The current investigation was taken to scrutinize the action of tranilast on the airway remodeling in chronic asthma in mice. MATERIALS AND METHODS:Intraperitoneal injection of ovalbumin was applied to mice for sensitization and subsequent inhalation of 1% ovalbumin three times week for 10 weeks for c...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2016.1207727
更新日期:2016-08-01 00:00:00
abstract::In vitro responses of potential target cell types to air pollutants under physiological conditions may be useful in understanding the health effects of air pollution exposure. The study evaluated responses of human primary airway epithelial cells to diesel exhaust (DE). Cultures of cells from 3 donors, differentiated ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601113088
更新日期:2007-01-01 00:00:00
abstract::The effects of urban air and diesel particles on inflammatory cytokine gene expression, tumor necrosis factor alpha (TNF-alpha) in particular, were studied in rat alveolar macrophages. TNF-alpha, interleukin (IL)-1, IL-6, cytokine-induced neutrophil chemoattractant (CINC), and macrophage inflammatory protein (MIP)-2 g...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149609046043
更新日期:1996-09-01 00:00:00
abstract::We investigated the distribution of adrenergic receptors in ferret trachea using autoradiography. [3H]Dihydroalprenolol, used to identify beta-adrenoceptors, revealed a high density of specific binding sites over surface epithelium and submucosal glands, with less labelling of smooth muscle. [3H]prazosin labelling sho...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061513
更新日期:1983-11-01 00:00:00
abstract::The synthesis of proteoglycans by primary cultures of 19-day gestation fetal rat lung Type II pneumonocytes was studied. The cells were grown in the presence of [3H]-glucosamine and/or [35S]-Na2SO4 and the radioactive label incorporated into proteoglycans was analyzed. Proteoglycans of high molecular weight (approxima...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064304
更新日期:1987-01-01 00:00:00
abstract::The objective of this study was to assess the radioprotective effects of amifostine in the rat model of radiation-induced lung injury using fractionated doses of radiation, to determine whether amifostine given before irradiation protects tumor from radiation cytotoxicity, and to determine whether changes in plasma le...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290096791
更新日期:2002-10-01 00:00:00
abstract::Immunosuppressive and antiproliferative effects of heparin may be beneficial in the field of solid organ transplantation. The aim of this study was to examine the effect of low-molecular-weight heparin (LMWH) compounds on the development of obliterative airway disease (OAD) in the rat tracheal transplant model. Alloge...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.499443
更新日期:2010-12-01 00:00:00
abstract::The aim of the study was to identify the effects on the lung after subchronic glass fiber intratracheal instillation study on rats. We evaluated the toxicological effects on the lung: persistent inflammatory reaction, cell proliferation, and pulmonary fibrosis on histopathological examination. We performed a glass fib...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.860568
更新日期:2014-03-01 00:00:00
abstract::Mechanical strain initiates a variety of responses in pulmonary epithelial cells. The signaling pathways and molecular alterations leading to these responses remain unclear To identify novel signal transduction pathways activated by strain, macroarray analysis was performed on strained pulmonary epithelial cells. Glut...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490517782
更新日期:2004-12-01 00:00:00
abstract::Understanding the effects from long-term exposure to individual ambient air pollutants and mixtures of pollutants is necessary for adequate assessment of health risk. This study examined quantitative and temporal alterations in tracheobronchial mucociliary clearance function and bronchial epithelial secretory cells in...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209064343
更新日期:1992-07-01 00:00:00
abstract:INTRODUCTION:Nintedanib is a multi-tyrosine kinase receptor inhibitor recently approved for treatment of idiopathic pulmonary fibrosis. Although angiogenesis is a key process involved in airway structural changes in patients with bronchial asthma, the effect of nintedanib targeting the angiokinase pathway on airway inf...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1339141
更新日期:2017-01-01 00:00:00
abstract::Neutrophilic inflammation is responsible for much of the tissue damage seen in many lung diseases. For resolution of inflammation to occur, neutrophils must die by apoptosis, allowing their recognition and removal by macrophages. The molecular events controlling this important regulatory step are poorly understood, in...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.1080/01902140701756778
更新日期:2007-12-01 00:00:00
abstract::In order to investigate some of the characteristics of superoxide anion release from alveolar macrophages, the effects of substances known to influence superoxide release from polymorphonuclear leukocytes (PMN) were studied in rat alveolar macrophages. There is a relatively small, but constant, amount of superoxide re...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052305
更新日期:1981-05-01 00:00:00
abstract:PURPOSE:This study aimed to investigate differential cell responses of alveolar epithelial cells (AECs) after treatments with lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) to mimic the exposure to inflammation and oxidative stress and the mechanisms of a double-hit model of apoptosis. MATERIALS AND METHODS:AEC...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1368738
更新日期:2017-10-01 00:00:00
abstract::In vitro observations of fetal lung maturation are complicated by continued epithelial cell maturation in culture. Removal of trace quantities of cortisol from the culture system has been reported elsewhere by Torday to prevent epithelial cell differentiation, but also prevent cell growth. In this report we have devel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061503
更新日期:1983-07-01 00:00:00
abstract::Investigators disagree on the amount of hydrogen peroxide (H2O2) released by resting and stimulated alveolar macrophages. The method commonly used to measure H2O2 release involves horseradish peroxidase (HRP)-catalyzed oxidation of scopoletin by H2O2. We describe an artifact in this method that may explain the seeming...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087899
更新日期:1984-01-01 00:00:00
abstract::The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701703333
更新日期:2007-10-01 00:00:00
abstract::To better understand the mechanisms by which neutrophils migrate into the airways, we constructed a novel in vitro model system with human umbilical vein endothelial cell (HUVE) monolayers grown on top of permeable filters and human lung Type II-like alveolar epithelial cell (A549) monolayers grown on the undersurface...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809099591
更新日期:1998-11-01 00:00:00
abstract::Purpose/Aim of Study: Studies of pulmonary fibrosis (PF) have resulted in DNA damage, inflammatory response, and cellular senescence being widely hypothesized to play a role in the progression of the disease. Utilizing these aforementioned terms, genomics databases were interrogated along with the term, "pulmonary fib...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1318975
更新日期:2017-04-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::We hypothesized that replication-deficient adenovirus (Ad), when complexed with plasmid DNA (pl) and cationic liposomes (L), would enhance liposome-mediated gene transfer in cultured human airway epithelial cells. Pl/L/Ad complexes were formed using charge-charge interactions. A gel electrophoresis retardation assay s...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021400269862
更新日期:2000-04-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is characterized by systemic and local chronic inflammation and oxidative stress. The sources of the increased oxidative stress in COPD patients derive from the increased burden of inhaled oxidants such as cigarette smoke and other forms of particulate or gaseous air pollut...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148.2011.580416
更新日期:2011-08-01 00:00:00
abstract::The authors recently showed that preterm birth per se, in the absence of assisted ventilation or elevated inhaled oxygen levels, alters the structure of the airway walls in young lambs. The initial aim of the present study was to determine whether these changes persist into adulthood. Preterm (P; n = 7) lambs were del...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701807720
更新日期:2008-02-01 00:00:00