Abstract:
:The effects of urban air and diesel particles on inflammatory cytokine gene expression, tumor necrosis factor alpha (TNF-alpha) in particular, were studied in rat alveolar macrophages. TNF-alpha, interleukin (IL)-1, IL-6, cytokine-induced neutrophil chemoattractant (CINC), and macrophage inflammatory protein (MIP)-2 gene expression and TNF-alpha secretion were increased in cells treated with 50 to 200 micrograms/mL of urban air particles in a concentration-related manner. There was no cytokine induction by diesel particles at any of the concentrations tested. Cytokine expression was not related to reactive oxygen species since antioxidants, such as catalase, TMTU, or DMSO, had no effect on TNF-alpha secretion. However, cytokine induction by urban air particles was completely prevented by polymyxin B, an antibiotic capable of neutralizing bacterial lipopolysaccharide (LPS) activities. Furthermore, LPS was detected on the urban air particles, but not on diesel particle. These results suggest that activation of cytokine gene expression and secretion in rat alveolar macrophages by urban air particles is due to the presence of endotoxin on the particles.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Dong W,Lewtas J,Luster MIdoi
10.3109/01902149609046043subject
Has Abstractpub_date
1996-09-01 00:00:00pages
577-92issue
5eissn
0190-2148issn
1521-0499journal_volume
22pub_type
杂志文章abstract::Diesel exhaust particles (DEP) are associated with respiratory disease and exposure to diesel exhaust induces an inflammatory response associated with marked leukocytic infiltration in the lung. This study examined whether neutrophils are activated by the active component of DEP (methanol extract of DEP [me-DEP]). The...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601047641
更新日期:2006-10-01 00:00:00
abstract::Protein tyrosine phosphatase is an important class of enzymes that plays an essential role in the cellular proliferation, differentiation, and oncogenesis. In this paper we report characterization of a low-molecular-weight protein tyrosine phosphatase purified from bovine lung. The enzyme activity was essentially inde...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809041534
更新日期:1998-05-01 00:00:00
abstract::The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701539745
更新日期:2007-08-01 00:00:00
abstract::Sirius Red staining is presented as a method for collagen determination, enabling quantitative morphometric measurements to be performed in locally defined tissue areas. The advantage of this method is especially shown for alveolar lung tissue. By excluding the bronchial areas in the tissue sections, the differences i...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031745
更新日期:1995-01-01 00:00:00
abstract:AIM OF THE STUDY:Nucleic acid-based therapies have the potential to provide clinically meaningful benefit across a wide spectrum of lung disease. However, in vivo delivery remains a challenge. Here we examined the feasibility of using electrospray to deliver nucleic acids to both porcine tracheal tissue sections and wh...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1563924
更新日期:2018-01-01 00:00:00
abstract::A past study demonstrated that all-trans-retinoic acid (ATRA) treatment by intraperitoneal injection in a rat model of elastase-induced emphysema caused tissue regeneration as evidenced by a decrease in alveolar size and lung volume and an increase in alveolar number. We postulated that treatment with this retinoid by...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490463142
更新日期:2004-07-01 00:00:00
abstract::Lung cancer, specifically non-small cell lung cancer (NSCLC), is a leading cause of mortality worldwide. In China, a dramatic increase in the incidence of NSCLC is expected in the next 20 years (Molina et al. Mayo Clin Proc. 2008;83:584–594). Mutated epidermal growth factor receptor (EGFR) status is a known predictor ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.482176
更新日期:2010-11-01 00:00:00
abstract::To better understand the mechanisms by which neutrophils migrate into the airways, we constructed a novel in vitro model system with human umbilical vein endothelial cell (HUVE) monolayers grown on top of permeable filters and human lung Type II-like alveolar epithelial cell (A549) monolayers grown on the undersurface...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809099591
更新日期:1998-11-01 00:00:00
abstract::The role of the Clara and type II cell in the development of pulmonary tumors in the A/J mouse and Fischer rat was investigated by determining the relationship of DNA methylation and repair in pulmonary cells to oncogene activation and by characterizing the morphology of pulmonary tumors induced by treatment with 4-(m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064417
更新日期:1991-03-01 00:00:00
abstract:AIM OF THE STUDY:The current investigation was taken to scrutinize the action of tranilast on the airway remodeling in chronic asthma in mice. MATERIALS AND METHODS:Intraperitoneal injection of ovalbumin was applied to mice for sensitization and subsequent inhalation of 1% ovalbumin three times week for 10 weeks for c...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2016.1207727
更新日期:2016-08-01 00:00:00
abstract::Human and canine airway mucosal explants synthesize and secrete high molecular weight glycoconjugates, incorporating 14C-glucosamine, a radioactive precursor to epithelial glycoprotein. Our examination of secretions produced by several individual specimens, however, did not reveal epithelial glycoprotein of typical bu...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148609058290
更新日期:1986-01-01 00:00:00
abstract::Despite extensive research and clinical efforts made in the management of acute pancre-atitis during the past few decades, to date no effective cure is available and the mortality from severe acute pancre-atitis remains high. Given that lung is the primary cause of early death in acute pancreatitis patients, novel the...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2014.908249
更新日期:2014-08-01 00:00:00
abstract::Few studies are available about the role of dietary zinc (Zn) in respiratory diseases. Adult male rats were divided into 2 groups and fed respectively a moderate Zn-deficient diet and a Zn-adequate control diet. In lung tissue at 2 months, thiobarbituric acid-reactive species (TBARS), total glutathione, glutathione di...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140303776
更新日期:2003-10-01 00:00:00
abstract::We studied lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA), plasma concentrations of 6-keto-PGF1 alpha and thromboxane B2, and pulmonary edema as indices of lung injury in rabbits exposed to cigarette smoke (CSE). Forty-six rabbits were randomly assigned to 4 groups: co...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064313
更新日期:1987-01-01 00:00:00
abstract::Both hyperoxia-induced proapoptotic sensitization of alveolar type II cells (TII cells) and high-stretch mechanical ventilation induced pulmonary inflammation are tumor necrosis factor alpha (TNFalpha) mediated. Therefore, binding of free TNFalpha should protect from TNFalpha-mediated acute lung injury and ameliorate ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902887430
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Hypoperfusion of the pulmonary vascular bed under the condition of congenital cardiac malformations may lead to progressive pulmonary vascular disease. To improve the mechanistic understanding of this disease, we examined the biochemical and morphological changes of the lung in a relevant animal model and pr...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.708385
更新日期:2012-09-01 00:00:00
abstract::We set out to determine whether or not the "mucolytic" drug N-acetylcysteine would speed the reversal of cigarette smoke-induced secretory-cell hyperplasia to normal, similar to that found previously for two nonsteroidal antiinflammatory drugs. Cigarette smoke alone significantly (p less than 0.01) increased the numbe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809062848
更新日期:1988-01-01 00:00:00
abstract::We have previously shown that intratracheally instilled silica (quartz) produces both morphologic evidence of emphysema and small-airway changes, and functional evidence of airflow obstruction. To further define the nature of the airflow-associated lesions induced by silica, we compared silica-exposed rats to rats giv...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069628
更新日期:1989-12-01 00:00:00
abstract::Neutrophilic inflammation is responsible for much of the tissue damage seen in many lung diseases. For resolution of inflammation to occur, neutrophils must die by apoptosis, allowing their recognition and removal by macrophages. The molecular events controlling this important regulatory step are poorly understood, in...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.1080/01902140701756778
更新日期:2007-12-01 00:00:00
abstract::In order to study the kinetics and pathways of protein transfer in pleural effusion, rats with pleurisy associated with hyperoxic pulmonary edema were injected either intrapleurally or intravenously with tracers. 125I-Albumin was used to obtain quantitative data. Anti horseradish peroxidase used as a morphological tra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148609057501
更新日期:1986-01-01 00:00:00
abstract::Excess production of neutrophil elastase contributes to the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the role of neutrophil elastase in the repair process following ALI/ARDS is not well understood. The objective of this study was to evaluate the effect of neutrop...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2011.633306
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Pulmonary ischemia-reperfusion (IR) is a biopathological event detectable in several clinical conditions, including lung transplantation, cardiopulmonary bypass, resuscitation, and pulmonary embolism. The understanding behind the activation of various inflammatory mediators regulating the apoptotic pathways ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.858196
更新日期:2014-02-01 00:00:00
abstract::The effects of Zizyphi fructus, a major constituent of Chinese anti-asthmatic herbal medicine, on ciliary motility and nitric oxide (NO) generation in canine cultured tracheal epithelium were studied by the microphoto-oscillation method and the specific amperometric method, respectively. Addition of Zizyphi fructus at...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149609031774
更新日期:1996-05-01 00:00:00
abstract::Lung cancer is the leading cause of death of both men and women across the world. Overexpression and activating mutations of the epidermal growth factor receptor-1 (EGFR1) are frequently observed and associated with poor prognosis. To inhibit the function of EGFR1, multiple antibodies and small-molecule tyrosine kinas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.831960
更新日期:2013-11-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is characterized by systemic and local chronic inflammation and oxidative stress. The sources of the increased oxidative stress in COPD patients derive from the increased burden of inhaled oxidants such as cigarette smoke and other forms of particulate or gaseous air pollut...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148.2011.580416
更新日期:2011-08-01 00:00:00
abstract::Angiotensin-converting enzyme (ACE) generates angiotensin II and degrades bradykinin. This ectopeptidase acts as a marker in pathologic conditions, showing tissue and vascular damage. In order to evaluate serum and tissue ACE activity in bleomycin-associated lung damage, the authors studied morphological and enzymatic...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490266556
更新日期:2004-03-01 00:00:00
abstract::In a number of recent studies, a lung tumor resistance locus designated either Par2 or Pas7 was mapped to distal chromosome 18 in crosses between susceptible A/J and more resistant BALB/c mice. This locus is important in that it accounts for as much as 60% of the difference in lung tumor susceptibility between the A/J...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140150216710
更新日期:2000-12-01 00:00:00
abstract::Survival of mice after Klebsiella pneumoniae infection and phagocytosis by alveolar macrophages (AMs), in the presence or absence of ozone (O(3)) exposure prior to infection, is sex dependent. The objective of this work was to study the role of gonadal hormones, 5α-dihydrotestosterone (DHT) and 17β-estradiol (E(2)), o...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2011.654045
更新日期:2012-05-01 00:00:00
abstract::During lung development, the extracellular matrix undergoes dynamic remodeling. Matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs), are important enzymes that participate in regulating tissue remodeling. There is an abnormal balance of the synthesis and degradation of collagen...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140303783
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:Inhaled capsaicin (8-methyl-N-vanillyl-6-nonenamide) has been used to induce cough in a safe and dose-dependent manner. Chronic cough is associated with an increased sensitivity to inhaled capsaicin in patients with asthma. The aim of this study was to evaluate clinical impact of capsaicin provocation test f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1834016
更新日期:2021-02-01 00:00:00