Abstract:
:We studied lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA), plasma concentrations of 6-keto-PGF1 alpha and thromboxane B2, and pulmonary edema as indices of lung injury in rabbits exposed to cigarette smoke (CSE). Forty-six rabbits were randomly assigned to 4 groups: control sham smoke exposure (SS, N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N = 10), CSE (N = 9), sham smoke exposure ibuprofen-pretreated (SS-I, N = 10), CSE (N = 9), and CSE ibuprofen-pretreated (CSE-I, N = 19). Ibuprofen (cyclooxygenase eicosanoid inhibitor) was administered as a single daily intramuscular injection (25 mg/kg) for 7 days before the experiment. Cigarette or sham smoke was delivered by syringe in a series of 5, 10, 20, and 30 tidal volume breaths with a 15-min counting period between each subset of breaths to determine 99mTcDTPA biological half-life (T1/2). In the ibuprofen pretreated group, CSE caused significant decreases in 99mTcDTPA T1/2 and dynamic lung compliance. Furthermore, these changes in lung function were accompanied by severe injury to type I alveolar cell epithelium, pulmonary edema, and frequently death of the rabbits. These findings suggest that inhibition of the cyclooxygenase pathway before CSE exacerbates lung injury in rabbits.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Witten ML,Lemen RJ,Quan SF,Sobonya RE,Magarelli JL,Bruck DCdoi
10.3109/01902148709064313subject
Has Abstractpub_date
1987-01-01 00:00:00pages
113-26issue
2eissn
0190-2148issn
1521-0499journal_volume
13pub_type
杂志文章abstract:INTRODUCTION:Nintedanib is a multi-tyrosine kinase receptor inhibitor recently approved for treatment of idiopathic pulmonary fibrosis. Although angiogenesis is a key process involved in airway structural changes in patients with bronchial asthma, the effect of nintedanib targeting the angiokinase pathway on airway inf...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1339141
更新日期:2017-01-01 00:00:00
abstract::The objective of this study was to assess the radioprotective effects of amifostine in the rat model of radiation-induced lung injury using fractionated doses of radiation, to determine whether amifostine given before irradiation protects tumor from radiation cytotoxicity, and to determine whether changes in plasma le...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290096791
更新日期:2002-10-01 00:00:00
abstract::A specific form of flavin monooxygenase has been identified in the lungs of a number of species. Distribution of the pulmonary flavin-containing monooxygenase (FMOp) is of interest because it oxidatively metabolizes a wide variety of nitrogen-, sulfur-, and phosphorous-containing xenobiotics, some of which form highly...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209020656
更新日期:1992-01-01 00:00:00
abstract::Chymase is a chymotrypsin-like serine protease that is present in mast cells. Its activities include various effects associated with inflammatory responses. But little is known about the effects of chymase in pulmonary fibrosis. The mouse silicosis model was induced by intratracheal injection of 10 mg silica. The Ashc...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.520815
更新日期:2011-03-01 00:00:00
abstract::The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of primary importance. Various pathological processes affect the extracellular matrix (ECM...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.1080/019021490944232
更新日期:2005-07-01 00:00:00
abstract::Diesel exhaust particles (DEP) are associated with respiratory disease and exposure to diesel exhaust induces an inflammatory response associated with marked leukocytic infiltration in the lung. This study examined whether neutrophils are activated by the active component of DEP (methanol extract of DEP [me-DEP]). The...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601047641
更新日期:2006-10-01 00:00:00
abstract::Mesothelial cells proliferate soon after asbestos deposition in the lung. The present study investigates whether the known mesothelial cell mitogens keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) are present in the lung and specifically in the pleural cavity during the phase of mesothelial cell gr...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401753181854
更新日期:2001-10-01 00:00:00
abstract::Endothelin-1 (ET-1) has constrictor and mitogenic effects on airway smooth muscle strips and cultured cells, respectively. This study addresses the type of the ET receptor subtype(s) present on ovine airway smooth muscle cells and the possibility of autocrine effects. The expression of the preproendothelin-1 gene was ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509023719
更新日期:1995-05-01 00:00:00
abstract::The role of the Clara and type II cell in the development of pulmonary tumors in the A/J mouse and Fischer rat was investigated by determining the relationship of DNA methylation and repair in pulmonary cells to oncogene activation and by characterizing the morphology of pulmonary tumors induced by treatment with 4-(m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064417
更新日期:1991-03-01 00:00:00
abstract:PURPOSE:This study aimed to investigate differential cell responses of alveolar epithelial cells (AECs) after treatments with lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) to mimic the exposure to inflammation and oxidative stress and the mechanisms of a double-hit model of apoptosis. MATERIALS AND METHODS:AEC...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1368738
更新日期:2017-10-01 00:00:00
abstract::Thrombin activates protease-activated receptor (PAR)-1 and induces a myofibroblast phenotype in normal lung fibroblasts. The origins of myofibroblasts are resident fibroblasts, fibrocytes, and epithelial-mesenchymal transition (EMT). We investigated the effects of thrombin, an important mediator of interstitial lung f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.820809
更新日期:2013-10-01 00:00:00
abstract::Leptin, an adipocyte-secreted hormone that regulates food intake and metabolic response, has been recently reported to increase in the serum during inflammatory airway disease associated with mucus-hypersecretion. We investigated the effects of leptin on mucin expression in human airway epithelial cells and the signal...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140903427033
更新日期:2010-06-01 00:00:00
abstract::We compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. Judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. Lung volumes in both air- and saline-filled lungs and mean chord length (Lm) were incre...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069638
更新日期:1989-12-01 00:00:00
abstract::The percentage 24-h lung retention of 4-micrometers monodispersed Teflon particles, aerodynamic diameter about 6 micrometers, was studied twice in 8 healthy nonsmokers. The particles were inhaled at 0.5 liter/sec with maximally deep breaths. Bronchoconstriction was induced by inhalation of a methacholine-bromide aeros...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087917
更新日期:1984-01-01 00:00:00
abstract::Administering liposome-encapsulated drugs by aerosols could be a feasible way of targeting drugs to the lung, specifically to pulmonary alveolar macrophages (AM). In the mouse model, we characterized uptake of carboxyfluorescein- (CF-) labeled liposomes by AM in vivo after acute inhalation of liposome aerosols, and th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309071077
更新日期:1993-01-01 00:00:00
abstract::It has been suggested that increased recruitment of phagocytes and subsequent clearance of particles may follow instillation of a leukocyte chemoattractant to lungs containing silica. The present study quantitated serially the silica content in alveolar spaces, in lung tissue and in hilar lymph nodes of mice that rece...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149409064384
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:Hypoperfusion of the pulmonary vascular bed under the condition of congenital cardiac malformations may lead to progressive pulmonary vascular disease. To improve the mechanistic understanding of this disease, we examined the biochemical and morphological changes of the lung in a relevant animal model and pr...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.708385
更新日期:2012-09-01 00:00:00
abstract::Previously, the authors have shown that tracheal occlusion (TO) partially reverses the onset of congenital diaphragmatic hernia (CDH)-induced pulmonary hypertension (PH) and abnormal pulmonary vascular development whereas release of the occlusion (TR) abolishes these clinical benefits. As a consequence of their mitoge...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021490927079
更新日期:2005-05-01 00:00:00
abstract::Aberrant expression of key cell cycle regulatory genes is essential for the immortalization and transformation of cells in vitro. We examined 20 mouse lung epithelial cell lines (2 nontumorigenic, 5 nonmetastatic, and 13 metastatic) for mutations or alterations in the expression of key components of the Rb pathway (pR...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401300054064
更新日期:2001-04-01 00:00:00
abstract::This report describes the protocol for the strain A mouse lung tumor bioassay and summarizes results on selected chemicals that have been tested for carcinogenicity in the assay. The assay is of 6 months duration and can distinguish 2-fold differences in carcinogenic potential of compounds from several chemical classe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064428
更新日期:1991-03-01 00:00:00
abstract::Protein tyrosine phosphatase is an important class of enzymes that plays an essential role in the cellular proliferation, differentiation, and oncogenesis. In this paper we report characterization of a low-molecular-weight protein tyrosine phosphatase purified from bovine lung. The enzyme activity was essentially inde...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809041534
更新日期:1998-05-01 00:00:00
abstract::Both hyperoxia-induced proapoptotic sensitization of alveolar type II cells (TII cells) and high-stretch mechanical ventilation induced pulmonary inflammation are tumor necrosis factor alpha (TNFalpha) mediated. Therefore, binding of free TNFalpha should protect from TNFalpha-mediated acute lung injury and ameliorate ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902887430
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:We have previously reported that N-acetylcysteine (NAC), ambroxol and azithromycin (AZM) (partially) correct the chloride efflux dysfunction in cystic fibrosis bronchial epithelial (CFBE) cells with the ΔF508 homozygous mutation in vitro. METHODS:In the present paper, we further investigated p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2014.934411
更新日期:2015-06-01 00:00:00
abstract::Recently there has been a great deal of interest in exploring possible ways to protect the lung from oxidant damage. Since sulfhydryl compounds are among the most important endogenous antioxidants, their therapeutic use has been proposed. Glutathione (GSH), the main intracellular nonprotein sulfhydryl, plays an import...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148809064189
更新日期:1988-01-01 00:00:00
abstract::Intraphagosomal pH in rabbit alveolar macrophages was studied using amorphous silica particles (FSP) and yeast particles (FYP) labeled with fluorescein. The pH was estimated from the quotient between the fluorescence intensity at wavelength 519 nm with excitation at wavelengths 495 and 450 nm. Within a factor of 10, p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069608
更新日期:1989-01-01 00:00:00
abstract::Urban air consists of a combination of environmental pollutants. Recent studies have suggested that normally innocuous doses of a particular pollutant may be rendered more toxic to the lung if primed by earlier events. Pulmonary inflammation has been observed in humans and in many animal species after endotoxin and oz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290092029
更新日期:2002-09-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is characterized by systemic and local chronic inflammation and oxidative stress. The sources of the increased oxidative stress in COPD patients derive from the increased burden of inhaled oxidants such as cigarette smoke and other forms of particulate or gaseous air pollut...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148.2011.580416
更新日期:2011-08-01 00:00:00
abstract::Lung cancer, specifically non-small cell lung cancer (NSCLC), is a leading cause of mortality worldwide. In China, a dramatic increase in the incidence of NSCLC is expected in the next 20 years (Molina et al. Mayo Clin Proc. 2008;83:584–594). Mutated epidermal growth factor receptor (EGFR) status is a known predictor ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.482176
更新日期:2010-11-01 00:00:00
abstract::ABSTRACT During fetal development physiological stretching helps drive lung growth and maturation. At birth, the α-subunit of the alveolar epithelial sodium channel (α-ENaC) is a critical factor in helping to facilitate clearance of lung fluid during the perinatal period. The effects of stretch, however, on α-ENaC exp...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2014.934410
更新日期:2014-10-01 00:00:00
abstract::Suboptimal intake of dietary zinc (Zn) is one of the most common nutritional problems worldwide. Previously, the authors have shown that zinc deficiency (ZD) produces oxidative and nitrosative stress in lung of male rats. The goal of this study is to test the effect of moderate ZD on insulin-like growth factor (IGF)-1...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140903061787
更新日期:2010-02-01 00:00:00