Abstract：
:ABSTRACT During fetal development physiological stretching helps drive lung growth and maturation. At birth, the α-subunit of the alveolar epithelial sodium channel (α-ENaC) is a critical factor in helping to facilitate clearance of lung fluid during the perinatal period. The effects of stretch, however, on α-ENaC expression in the fetal lung have yet to be elucidated. In an effort to explore this question, we used both an in vitro cell culture model that exposes cells to repetitive cyclic stretch (CS) as well as an in vivo preterm animal model of mechanical ventilation (MV). We found that murine lung epithelial (MLE-12) cells exposed to repetitive CS showed a significant rise in α-ENaC mRNA expression. Total and cell-surface protein abundance of α-ENaC were also elevated after 24 h of CS. Stretch-induced increases in α-ENaC expression were suppressed in the presence of either actinomycin D or cycloheximide. Pharmacological inhibition of the extracellular signal-regulated protein kinase (ERK1/2) did not attenuate stretch-induced increases in α-ENaC protein, whereas inhibition of p38 MAPK or c-Jun NH2-terminal kinase (JNK) did. In 29-day preterm rabbits, alveolar stretching secondary to postnatal MV markedly elevated fetal lung α-ENaC expression compared to spontaneously breathing counterparts. In summary, our findings indicate that mechanical stretch promotes α-ENaC expression.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Mustafa SB,Isaac J,Seidner SR,Dixon PS,Henson BM,DiGeronimo RJdoi
10.3109/01902148.2014.934410subject
Has Abstractpub_date
2014-10-01 00:00:00pages
380-91issue
8eissn
0190-2148issn
1521-0499journal_volume
40pub_type
杂志文章abstract：BACKGROUND AND PURPOSE:Serum amyloid A (SAA), an acute-phase protein whose level tracks infection and inflammation, is the precursor protein of amyloid A (AA) fibrils that is thought to cause AA amyloidosis in human and animals. SAA protein has several isoforms based on the difference of amino acid sequence, such as SA...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2020.1809750
更新日期：2020-11-01 00:00:00
abstract：:To study the pattern of lymphokines in bleomycin-induced lung injury, T cells were isolated from lung interstitial tissue (LIL), peribronchial lymphatic tissue (PBLT), and bronchoalveolar lavage (BAL) fluid of bleomycin-"sensitive" C57Bl/6 and bleomycin-"resistant" BALB/c mice at 3, 6, and 14 days following intratrach...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/019021400750048072
更新日期：2000-10-01 00:00:00
abstract：BACKGROUND:Bronchopulmonary dysplasia (BPD) is a main chronic lung disease commonly occurs in preterm infants. BPD is characterized by impaired alveolarization and vascularization of the developing lung. Transforming growth factor-β (TGF-β) signaling pathway is known to play an important role during lung vascular devel...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2016.1226448
更新日期：2016-09-01 00:00:00
abstract：:Emerging data indicate that endothelial-mesenchymal transition (EndMT) is involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). A previous study noted that blocking the activity of protein phosphatase 2 A (PP2A) could attenuate EndMT. However, the treatment effects of PP2A inhibitors in pulmonary fibros...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2020.1774823
更新日期：2020-09-01 00:00:00
abstract：:It is evident that much remains to be learned about the nasal passages and their responses to toxic materials. For the nose of both laboratory animals and humans, information is needed in the areas of anatomy, physiology, biochemistry, neurobiology, physiopathology, and oncology. This article briefly discussed toxic a...
journal_title：Experimental lung research
pub_type： 杂志文章,评审
doi：10.3109/01902149109064322
更新日期：1991-09-01 00:00:00
abstract：BACKGROUND:Inflammation is believed to play a key role in the pathophysiology of meconium aspiration syndrome (MAS). PURPOSE OF THE STUDY:The objective was to determine whether the recombinant human Erythropoietin (rhEPO) pretreatment could attenuate meconium-induced inflammation. MATERIALS AND METHODS:In this study,...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2016.1190424
更新日期：2016-05-01 00:00:00
abstract：:The production of reactive oxygen species (ROS) during hyperoxia contribute to alveolar epithelial apoptosis. In the present study, the molecular mechanisms of oxidative stress-induced alveolar epithelial cell apoptosis were investigated. The cytoprotective effects of N-acetylcysteine (NAC) were evaluated. Treatments ...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902141003678582
更新日期：2010-08-01 00:00:00
abstract：:The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of primary importance. Various pathological processes affect the extracellular matrix (ECM...
journal_title：Experimental lung research
pub_type： 杂志文章,评审
doi：10.1080/019021490944232
更新日期：2005-07-01 00:00:00
abstract：:Two morphologic characteristics have been used to define the nonciliated bronchiolar epithelial cell: abundant agranular endoplasmic reticulum (AER) and membrane-bound avoid granules. To assess the ultrastructural homogeneity of this cell type in the lungs of large domestic mammals used as experimental models in pulmo...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148009069645
更新日期：1980-06-01 00:00:00
abstract：:The aim of this study was to determine influence of prenatal granulocyte-macrophage colony-stimulating factor (GM-CSF) administration on lung growth, maturation, and vascular endothelial growth factor (VEGF) expression. Twenty Wistar rats received sterile saline (1 mL) or recombinant human GM-CSF (50 micro g/kg) on da...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902140802341736
更新日期：2008-11-01 00:00:00
abstract：:Purpose/Aim of Study: Studies of pulmonary fibrosis (PF) have resulted in DNA damage, inflammatory response, and cellular senescence being widely hypothesized to play a role in the progression of the disease. Utilizing these aforementioned terms, genomics databases were interrogated along with the term, "pulmonary fib...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2017.1318975
更新日期：2017-04-01 00:00:00
abstract：BACKGROUND:This study aimed to develop and evaluate an adaptive control system for volume-controlled ventilation (VCV) in small animals to guarantee accurate delivery of tidal volume (VT) in the presence of changes in lung mechanics. METHODS:The adaptive control system to control the Harvard Inspira ventilator was des...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148.2014.900156
更新日期：2014-04-01 00:00:00
abstract：:The effects of urban air and diesel particles on inflammatory cytokine gene expression, tumor necrosis factor alpha (TNF-alpha) in particular, were studied in rat alveolar macrophages. TNF-alpha, interleukin (IL)-1, IL-6, cytokine-induced neutrophil chemoattractant (CINC), and macrophage inflammatory protein (MIP)-2 g...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902149609046043
更新日期：1996-09-01 00:00:00
abstract：:This study evaluated polymorphonuclear leukocyte (PMN) involvement in translocation of dust to bronchial lymph nodes after deposition of dust in the lungs of control and neutropenic F344/N rats. Rats were rendered neutropenic with an intraperitoneal (IP) injection of anti-rat PMN antiserum (APA); control rats were inj...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902149709046048
更新日期：1997-01-01 00:00:00
abstract：:TRPV1 is a modulator of noxious stimuli known to be important in the cough reflex. We have compared the expression of TRPV1 in normal human airways and those from patients with chronic cough and found that there is up regulation in airways smooth muscle in disease. This increased expression appears to be intracellular...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902140590918803
更新日期：2005-04-01 00:00:00
abstract：:Male Sprague-Dawley rats were placed in hypobaric hypoxia for 17-21 d (FIO2 10%) to establish pulmonary hypertension (PH) and control rats were kept in normobaric room air. Right mean atrial and ventricular pressures (PRA, PRV) were recorded, left ventricular (LV) blood was collected, and lungs were perfused with hepa...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902149209031681
更新日期：1992-03-01 00:00:00
abstract：AIM OF THE STUDY:Long-term exposure to cigarette smoke generates chronic obstructive pulmonary disease (COPD) in guinea pigs, but a comprehensive evaluation of changes in lung function, as assessed by barometric whole body plethysmography (WBP), is lacking. MATERIALS AND METHODS:Female guinea pigs were exposed to the ...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2017.1294632
更新日期：2017-03-01 00:00:00
abstract：:Volcanic ash was collected from the Moses Lake region of Washington State after the 18 May 1980 eruption of Mt. St. Helens. The ash was tested in a short-term bioassay system using hamsters exposed by intratracheal instillation. One day after exposure the lungs were lavaged and the fluid collected was characterized us...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148109052324
更新日期：1981-11-01 00:00:00
abstract：:COPD remains a public health problem that has yet to be fully recognized by the public, the medical community or public health (government) officials, and much work remains to be done worldwide to reverse the increasing trends of this chronic lung disease. ...
journal_title：Experimental lung research
pub_type： 杂志文章,评审
doi：
更新日期：2005-09-01 00:00:00
abstract：BACKGROUND:Hypoperfusion of the pulmonary vascular bed under the condition of congenital cardiac malformations may lead to progressive pulmonary vascular disease. To improve the mechanistic understanding of this disease, we examined the biochemical and morphological changes of the lung in a relevant animal model and pr...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148.2012.708385
更新日期：2012-09-01 00:00:00
abstract：:Alveolar cells were evaluated in BALB/c mice exposed to smoke from 9 cigarettes per day. The mice were sacrificed at 1, 5, and 10 days and examined by lung morphometry and immunohistochemical staining of alveolar macrophages for tumor necrosis factor (TNF)-alpha, matrix metalloproteinase (MMP)-12, and tissue inhibitor...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902140500324828
更新日期：2005-10-01 00:00:00
abstract：:Aims:Bronchopulmonary dysplasia (BPD) is characterized by alveolarization arrest. During alveolarization, alveolar myofibroblasts are thought to migrate into the septal tips and elongate secondary septa. Lipopolysaccharide (LPS) exposure has been reported to disrupt directional migration and final location of alveolar...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902148.2019.1711464
更新日期：2020-01-01 00:00:00
abstract：:The objective of this study was to evaluate the protective effects of resveratrol on lung injury in rats with severe acute pancreatitis. Ninety-six male Sprague-Dawley rats were randomly classified into 4 equal groups (n = 24): control, model, resveratrol-treated, and dexamethasone-treated. The rats were evaluated at ...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.1080/01902140902718197
更新日期：2009-05-01 00:00:00
abstract：:The effects of Zizyphi fructus, a major constituent of Chinese anti-asthmatic herbal medicine, on ciliary motility and nitric oxide (NO) generation in canine cultured tracheal epithelium were studied by the microphoto-oscillation method and the specific amperometric method, respectively. Addition of Zizyphi fructus at...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902149609031774
更新日期：1996-05-01 00:00:00
abstract：:In both ovalbumin-sensitized and ovalbumin-challenged guinea pigs, the phospholipid content of bronchoalveolar lavage fluid is decreased with respect to that of controls. In the sensitized guinea pig, the activity of lung membrane phospholipase is increased and the phospholipid content of lung membranes is decreased. ...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902149009087876
更新日期：1990-03-01 00:00:00
abstract：:Prostaglandins (PGs) synthesized by fetal and neonatal lungs play pivotal roles in pulmonary physiology, especially during the transition from uterine to independent life. One regulator of prostaglandin synthesis at this time may be oxygen. We examined the effects of 1% O2, 21% O2 and 50% O2 in 5% CO2, balance N2 (PO2...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148709064301
更新日期：1987-01-01 00:00:00
abstract：:Immunosuppressive and antiproliferative effects of heparin may be beneficial in the field of solid organ transplantation. The aim of this study was to examine the effect of low-molecular-weight heparin (LMWH) compounds on the development of obliterative airway disease (OAD) in the rat tracheal transplant model. Alloge...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148.2010.499443
更新日期：2010-12-01 00:00:00
abstract：:The inhalation of inorganic dust can lead to the development of interstitial pulmonary fibrosis, characterized by the accumulation of fibroblasts and connective tissue matrix in the lung interstitium. The fibrosis causes alterations in the architecture of the lung parenchyma, resulting in abnormal gas exchange and hyp...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902149109064332
更新日期：1991-11-01 00:00:00
abstract：:The morphology and phagocytic capacities of pulmonary macrophages recovered by lavage from normal rats were studied in vitro after incubation under various culture conditions. Most adherent macrophages (73 +/- 6%) were flat and exhibited ruffled surfaces. Phagocytosis of carbonyl iron beads by pulmonary macrophages wa...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148409087896
更新日期：1984-01-01 00:00:00
abstract：:Biochemical characterization of human respiratory mucus has generally utilized expectorated specimens. In order to exclude extraneous contaminants in the analysis of airway glycoproteins, human airways were cultured and the mucous glycoprotein released into the supernatant analyzed. By incorporating 3H-labeled glucosa...
journal_title：Experimental lung research
pub_type： 杂志文章
doi：10.3109/01902148409069675
更新日期：1984-01-01 00:00:00
