Abstract:
:Purpose/Aim of Study: Studies of pulmonary fibrosis (PF) have resulted in DNA damage, inflammatory response, and cellular senescence being widely hypothesized to play a role in the progression of the disease. Utilizing these aforementioned terms, genomics databases were interrogated along with the term, "pulmonary fibrosis," to identify genes common among all 4 search terms. Findings were compared to data derived from a model of radiation-induced progressive pulmonary fibrosis (RIPF) to verify that these genes are similarly expressed, supporting the use of radiation as a model for diseases involving PF, such as human idiopathic pulmonary fibrosis (IPF). MATERIALS AND METHODS:In an established model of RIPF, C57BL/6J mice were exposed to 12.5 Gy thorax irradiation and sacrificed at 24 hours, 1, 4, 12, and 32 weeks following exposure, and lung tissue was compared to age-matched controls by RNA sequencing. RESULTS:Of 176 PF associated gene transcripts identified by database interrogation, 146 (>82%) were present in our experimental model, throughout the progression of RIPF. Analysis revealed that nearly 85% of PF gene transcripts were associated with at least 1 other search term. Furthermore, of 22 genes common to all four terms, 16 were present experimentally in RIPF. CONCLUSIONS:This illustrates the validity of RIPF as a model of progressive PF/IPF based on the numbers of transcripts reported in both literature and observed experimentally. Well characterized genes and proteins are implicated in this model, supporting the hypotheses that DNA damage, inflammatory response and cellular senescence are associated with the pathogenesis of PF.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Beach TA,Johnston CJ,Groves AM,Williams JP,Finkelstein JNdoi
10.1080/01902148.2017.1318975subject
Has Abstractpub_date
2017-04-01 00:00:00pages
134-149issue
3eissn
0190-2148issn
1521-0499journal_volume
43pub_type
杂志文章abstract::Recently there has been a great deal of interest in exploring possible ways to protect the lung from oxidant damage. Since sulfhydryl compounds are among the most important endogenous antioxidants, their therapeutic use has been proposed. Glutathione (GSH), the main intracellular nonprotein sulfhydryl, plays an import...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148809064189
更新日期:1988-01-01 00:00:00
abstract:PURPOSE:As an angiotensin converting enzyme (ACE) inhibitor, the effects of captopril on inflammation has been previously examined. Captopril has been shown to have anti-inflammatory and antioxidant effects. Imbalance in the oxidant/antioxidant system is one of the major causes of inflammation. In the present study, th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1473530
更新日期:2018-01-01 00:00:00
abstract::Administering liposome-encapsulated drugs by aerosols could be a feasible way of targeting drugs to the lung, specifically to pulmonary alveolar macrophages (AM). In the mouse model, we characterized uptake of carboxyfluorescein- (CF-) labeled liposomes by AM in vivo after acute inhalation of liposome aerosols, and th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309071077
更新日期:1993-01-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::We hypothesized that replication-deficient adenovirus (Ad), when complexed with plasmid DNA (pl) and cationic liposomes (L), would enhance liposome-mediated gene transfer in cultured human airway epithelial cells. Pl/L/Ad complexes were formed using charge-charge interactions. A gel electrophoresis retardation assay s...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021400269862
更新日期:2000-04-01 00:00:00
abstract::Cyclin D1 dysregulation and differential inactivation of p16INK4a and Rb have been observed in human lung cancer. In chemically induced mouse lung tumors, the p16INK4a gene is a target of inactivation, and Rb is reduced at the mRNA level (Northern blot) although similar at the protein level (Western blot) when compare...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809087383
更新日期:1998-07-01 00:00:00
abstract::In a number of recent studies, a lung tumor resistance locus designated either Par2 or Pas7 was mapped to distal chromosome 18 in crosses between susceptible A/J and more resistant BALB/c mice. This locus is important in that it accounts for as much as 60% of the difference in lung tumor susceptibility between the A/J...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140150216710
更新日期:2000-12-01 00:00:00
abstract::Chlorinated amines are long-lived oxidants released from inflammatory cells. They have been observed to alter the function of important inflammatory molecules. Whether they can contribute directly to the inflammatory alteration of cellular barriers is not known. Monolayers of cultured porcine pulmonary artery endothel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087864
更新日期:1989-05-01 00:00:00
abstract::Angiotensin-converting enzyme (ACE) generates angiotensin II and degrades bradykinin. This ectopeptidase acts as a marker in pathologic conditions, showing tissue and vascular damage. In order to evaluate serum and tissue ACE activity in bleomycin-associated lung damage, the authors studied morphological and enzymatic...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490266556
更新日期:2004-03-01 00:00:00
abstract::The studies presented were designed to test the efficacy of farnesyltransferase inhibitors (FTIs) as potential chemopreventive compounds in the mouse lung tumor model, and in tumor cell lines. The compounds included manumycin, gliotoxin, dihydroepiandrosterone (DHEA), perillyl alcohol (POH), and FTI-276. Each of these...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140150216819
更新日期:2000-12-01 00:00:00
abstract::Lung injury and inflammation are associated with exposure to various types of particulate air pollutants. The present study was used to determine whether metalloproteinases (MMPs) are secreted after instilling dust samples into the lung, and to relate levels of specific MMPs to different fractions of the ambient air p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140303753
更新日期:2003-09-01 00:00:00
abstract::To study the pattern of lymphokines in bleomycin-induced lung injury, T cells were isolated from lung interstitial tissue (LIL), peribronchial lymphatic tissue (PBLT), and bronchoalveolar lavage (BAL) fluid of bleomycin-"sensitive" C57Bl/6 and bleomycin-"resistant" BALB/c mice at 3, 6, and 14 days following intratrach...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021400750048072
更新日期:2000-10-01 00:00:00
abstract::TRPV1 is a modulator of noxious stimuli known to be important in the cough reflex. We have compared the expression of TRPV1 in normal human airways and those from patients with chronic cough and found that there is up regulation in airways smooth muscle in disease. This increased expression appears to be intracellular...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140590918803
更新日期:2005-04-01 00:00:00
abstract::ABSTRACT During fetal development physiological stretching helps drive lung growth and maturation. At birth, the α-subunit of the alveolar epithelial sodium channel (α-ENaC) is a critical factor in helping to facilitate clearance of lung fluid during the perinatal period. The effects of stretch, however, on α-ENaC exp...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2014.934410
更新日期:2014-10-01 00:00:00
abstract::We investigated the distribution of adrenergic receptors in ferret trachea using autoradiography. [3H]Dihydroalprenolol, used to identify beta-adrenoceptors, revealed a high density of specific binding sites over surface epithelium and submucosal glands, with less labelling of smooth muscle. [3H]prazosin labelling sho...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061513
更新日期:1983-11-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) as a complex disease with genetic and environmental compound is one of the leading causes of death in worldwide. This disease is characterized by lower airway inflammation, and increases risk of lung cancer in smokers. Micro-RNA (miRNA) molecules are key regulators in gene ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.689088
更新日期:2012-08-01 00:00:00
abstract::This study describes the morphology of the adaptive response of the pulmonary parenchyma after the resection of lung tissue. Rats aged 23 days were subjected to the bilobectomy of the right upper and middle lobes representing 25% of the total lung volume. On the postoperative Days 1, 4, 6, 9, 12, 18, and 30, the lungs...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209039246
更新日期:1982-12-01 00:00:00
abstract::Lung cancer is the leading cause of death of both men and women across the world. Overexpression and activating mutations of the epidermal growth factor receptor-1 (EGFR1) are frequently observed and associated with poor prognosis. To inhibit the function of EGFR1, multiple antibodies and small-molecule tyrosine kinas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.831960
更新日期:2013-11-01 00:00:00
abstract::Freeze-fracture was used to study the nonciliated epithelial (Clara) cell of rat bronchiole. En face views of the P face of the luminal cell membrane reveal particle-free bulging and circular depressions at the presumptive sites of the membrane fusion between the cell membrane and secretory granule membrane. Cross fra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052313
更新日期:1981-08-01 00:00:00
abstract::The effect of glucocorticoids on the response of adenylate cyclase in fetal rat lung fibroblast and Type II epithelial cell cultures to beta-agonists and prostaglandin E2 (PGE2) was investigated. There was significant stimulation of cyclic AMP synthesis by adrenalin in both fibroblasts and Type II cells, which was pot...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087863
更新日期:1989-05-01 00:00:00
abstract::Purpose/Aim: More and more evidences suggest that airway remodeling of fibrotic lung diseases may be associated with epithelial-mesenchymal transition (EMT) of human A549 cells induced by transforming growth factor (TGF)-β1. Schisandrin B (Sch B) is the highest content of dibenzocyclooctadiene lignans in Schisandra ch...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2019.1631906
更新日期:2019-01-01 00:00:00
abstract::B6C3F1 female mice were exposed to cigarette smoke (CS) (250 mg/m3 total particulate material) or filtered air (FA), 6 hours/day, 5 days/week, for 6, 7, or 10 weeks, or to CS for 6 weeks, then FA for 1 or 4 additional weeks. Exposure to CS increased macrophages, neutrophils, lymphocytes, and matrix metalloproteinase (...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490252858
更新日期:2004-01-01 00:00:00
abstract::In vitro responses of potential target cell types to air pollutants under physiological conditions may be useful in understanding the health effects of air pollution exposure. The study evaluated responses of human primary airway epithelial cells to diesel exhaust (DE). Cultures of cells from 3 donors, differentiated ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601113088
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Nintedanib is a multi-tyrosine kinase receptor inhibitor recently approved for treatment of idiopathic pulmonary fibrosis. Although angiogenesis is a key process involved in airway structural changes in patients with bronchial asthma, the effect of nintedanib targeting the angiokinase pathway on airway inf...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1339141
更新日期:2017-01-01 00:00:00
abstract::Prostaglandins (PGs) synthesized by fetal and neonatal lungs play pivotal roles in pulmonary physiology, especially during the transition from uterine to independent life. One regulator of prostaglandin synthesis at this time may be oxygen. We examined the effects of 1% O2, 21% O2 and 50% O2 in 5% CO2, balance N2 (PO2...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064301
更新日期:1987-01-01 00:00:00
abstract::An IgM monoclonal antibody (1D9/B3) is characterized, which specifically recognizes basal cells of the upper airway epithelium. Although morphological features have been used to follow cell lineage and differentiation, an objective assessment of differentiation can be enhanced by characterizing the expression of speci...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031741
更新日期:1995-01-01 00:00:00
abstract:PURPOSE OF THE STUDY:Acute respiratory distress syndrome (ARDS) represents a major cause of mortality in intensive care patients. Activation of peroxisome proliferator-activated receptor-α (PPAR-α) by fibrates, such as WY-14643 (WY), has been described to beneficially influence inflammation and experimental lung injury...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1046200
更新日期:2015-01-01 00:00:00
abstract::The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701539745
更新日期:2007-08-01 00:00:00
abstract::The aim of this study was to determine influence of prenatal granulocyte-macrophage colony-stimulating factor (GM-CSF) administration on lung growth, maturation, and vascular endothelial growth factor (VEGF) expression. Twenty Wistar rats received sterile saline (1 mL) or recombinant human GM-CSF (50 micro g/kg) on da...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802341736
更新日期:2008-11-01 00:00:00
abstract::Sex-related differences may be present during fetal lung growth and at the onset of surfactant synthesis. In this study we investigated the role of dihydrotestosterone (DHT) and estrogen (EST) on cell division and on labeled palmitate incorporation into disaturated phosphatidylcholine (DSPC) at various times of gestat...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087851
更新日期:1989-03-01 00:00:00