Abstract:
:A specific form of flavin monooxygenase has been identified in the lungs of a number of species. Distribution of the pulmonary flavin-containing monooxygenase (FMOp) is of interest because it oxidatively metabolizes a wide variety of nitrogen-, sulfur-, and phosphorous-containing xenobiotics, some of which form highly toxic reactive intermediates. We have identified the nonciliated bronchiolar epithelial (Clara) cell as the predominant location for this enzyme in rabbit lung. In addition, protein in ciliated, endothelial, type I, and type II cells and in tracheal lining layer reacted with antibodies to FMOp. In all these cell types antigen was found associated with cytoplasmic organelles, and in the Clara cell antigen was most concentrated in areas rich in smooth endoplasmic reticulum. Staining of ciliated surfaces was also observed at both the light and electron microscopy levels. Extracellular antigen was also apparent in tracheal lining layer smeared onto glass slides. We compared the location of the FMOp with that of two enzymes of the cytochrome P-450 monooxygenase system (studied here and elsewhere), cytochrome P450 IIB (P450 IIB), and NADPH cytochrome P450 reductase (reductase), and concluded that (1) FMOp is detected in all cells where P450 IIB and reductase are both present (Clara, type II, and ciliated); (2) FMOp and P450 IIB, but not reductase, are detected in endothelial cells; (3) P450 IIB alone is detected in the plasma membrane, cilia, and microvillae of ciliated cells and plasma membrane of endothelial cells; and (4) FMOp alone is detected in type I cells.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Overby L,Nishio SJ,Lawton MP,Plopper CG,Philpot RMdoi
10.3109/01902149209020656subject
Has Abstractpub_date
1992-01-01 00:00:00pages
131-44issue
1eissn
0190-2148issn
1521-0499journal_volume
18pub_type
杂志文章abstract:PURPOSE:As an angiotensin converting enzyme (ACE) inhibitor, the effects of captopril on inflammation has been previously examined. Captopril has been shown to have anti-inflammatory and antioxidant effects. Imbalance in the oxidant/antioxidant system is one of the major causes of inflammation. In the present study, th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1473530
更新日期:2018-01-01 00:00:00
abstract::This report describes the protocol for the strain A mouse lung tumor bioassay and summarizes results on selected chemicals that have been tested for carcinogenicity in the assay. The assay is of 6 months duration and can distinguish 2-fold differences in carcinogenic potential of compounds from several chemical classe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064428
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abstract::The structural features of the ovine pleura are described using light, scanning, and transmission electron microscopy. Extensive sampling of the visceral and parietal pleura revealed considerable variation in both surface morphology and pleural interstitial anatomy. Variations of mesothelial surface were encountered a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309055016
更新日期:1983-05-01 00:00:00
abstract::In order to study the kinetics and pathways of protein transfer in pleural effusion, rats with pleurisy associated with hyperoxic pulmonary edema were injected either intrapleurally or intravenously with tracers. 125I-Albumin was used to obtain quantitative data. Anti horseradish peroxidase used as a morphological tra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148609057501
更新日期:1986-01-01 00:00:00
abstract::It has been suggested that increased recruitment of phagocytes and subsequent clearance of particles may follow instillation of a leukocyte chemoattractant to lungs containing silica. The present study quantitated serially the silica content in alveolar spaces, in lung tissue and in hilar lymph nodes of mice that rece...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149409064384
更新日期:1994-05-01 00:00:00
abstract::The aim of the study was to identify the effects on the lung after subchronic glass fiber intratracheal instillation study on rats. We evaluated the toxicological effects on the lung: persistent inflammatory reaction, cell proliferation, and pulmonary fibrosis on histopathological examination. We performed a glass fib...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.860568
更新日期:2014-03-01 00:00:00
abstract::A cast of human tracheobronchial airways, which is complete to airways less than 0.1 cm in diameter, was employed for experimental studies of gas convective transport by high-frequency ventilation (HFV). The cast was ventilated with gases of different kinematic viscosity (He, air, and SF6) and tidal volumes of 10-60 m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
abstract::Diesel exhaust particles (DEP) are associated with respiratory disease and exposure to diesel exhaust induces an inflammatory response associated with marked leukocytic infiltration in the lung. This study examined whether neutrophils are activated by the active component of DEP (methanol extract of DEP [me-DEP]). The...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601047641
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND:Inflammation is believed to play a key role in the pathophysiology of meconium aspiration syndrome (MAS). PURPOSE OF THE STUDY:The objective was to determine whether the recombinant human Erythropoietin (rhEPO) pretreatment could attenuate meconium-induced inflammation. MATERIALS AND METHODS:In this study,...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2016.1190424
更新日期:2016-05-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2017-01-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
abstract::Nicotine appears to be the primary pharmacologic agent that causes smoking-related pulmonary diseases. An understanding of the effect of nicotine on lungs is essential to develop interventions that can be used to counter smoking-related diseases. Further, it is shown that physical exercise may partially reverse smokin...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.666331
更新日期:2012-05-01 00:00:00
abstract::Expression of microRNAs (miRNAs) is characteristically altered in cancer, and they may play a role in cancer development and progression. The authors performed microarray and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses to determine the miRNA expression profile of primary sm...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902822312
更新日期:2009-10-01 00:00:00
abstract::Leukotrienes (LTs) are potent vasoconstrictors in the pulmonary circulation. We investigated LTB4 and LTE4 metabolism by intrapulmonary arteries and veins of 2 to 9 days old lambs (n = 6). Paired vessels were incubated under baseline, and stimulated conditions. LTB4 and LTE4 were extracted from media, quantfied by enz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401750193601
更新日期:2001-06-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is characterized by systemic and local chronic inflammation and oxidative stress. The sources of the increased oxidative stress in COPD patients derive from the increased burden of inhaled oxidants such as cigarette smoke and other forms of particulate or gaseous air pollut...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902148.2011.580416
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:Inhaled capsaicin (8-methyl-N-vanillyl-6-nonenamide) has been used to induce cough in a safe and dose-dependent manner. Chronic cough is associated with an increased sensitivity to inhaled capsaicin in patients with asthma. The aim of this study was to evaluate clinical impact of capsaicin provocation test f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1834016
更新日期:2021-02-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021400269862
更新日期:2000-04-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209064343
更新日期:1992-07-01 00:00:00
abstract::Few studies are available about the role of dietary zinc (Zn) in respiratory diseases. Adult male rats were divided into 2 groups and fed respectively a moderate Zn-deficient diet and a Zn-adequate control diet. In lung tissue at 2 months, thiobarbituric acid-reactive species (TBARS), total glutathione, glutathione di...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140303776
更新日期:2003-10-01 00:00:00
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journal_title:Experimental lung research
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doi:10.1080/019021499270376
更新日期:1999-03-01 00:00:00
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更新日期:2020-01-01 00:00:00
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journal_title:Experimental lung research
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更新日期:1998-07-01 00:00:00
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doi:10.3109/01902140903349562
更新日期:2010-05-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809099591
更新日期:1998-11-01 00:00:00
abstract::An improved method has been developed for separation of an enriched population of mouse type 2 pneumocytes, based on differential adherence and size fractionation of cells dissociated with trypsin. These cells were successfully maintained in primary culture in serum-free medium MCDB 201 supplemented with albumin, tran...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031746
更新日期:1995-01-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149109064322
更新日期:1991-09-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) as a complex disease with genetic and environmental compound is one of the leading causes of death in worldwide. This disease is characterized by lower airway inflammation, and increases risk of lung cancer in smokers. Micro-RNA (miRNA) molecules are key regulators in gene ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.689088
更新日期:2012-08-01 00:00:00
abstract::Three-day-old rabbits were given intratracheal injections of radiolabeled natural sheep surfactant, rabbit surfactant, or liposomes of dipalmitoylphosphatidylcholine that contained greater than three times the quantity of phosphatidylcholine present in the endogenous surfactant pool. The recoveries of radiolabeled pho...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148509057525
更新日期:1985-01-01 00:00:00
abstract::Freeze-fracture was used to study the nonciliated epithelial (Clara) cell of rat bronchiole. En face views of the P face of the luminal cell membrane reveal particle-free bulging and circular depressions at the presumptive sites of the membrane fusion between the cell membrane and secretory granule membrane. Cross fra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052313
更新日期:1981-08-01 00:00:00
abstract:PURPOSE:This study aimed to investigate differential cell responses of alveolar epithelial cells (AECs) after treatments with lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) to mimic the exposure to inflammation and oxidative stress and the mechanisms of a double-hit model of apoptosis. MATERIALS AND METHODS:AEC...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1368738
更新日期:2017-10-01 00:00:00