Abstract:
:Studies demonstrated that pathophysiological abnormalities of airway smooth muscle (ASM) contribute significantly to chronic obstructive pulmonary disease (COPD) pathogenesis, the aim of this study is to investigate heat shock protein 70 (Hsp70) in ASM in COPD. ASM from 8 COPD patients and 6 controls were isolated for detection of Hsp70 using Western blot. Male adult Wister rats were exposed to mixture of cigarette smoke/air or room air for an indicated period. The lung tissues were obtained for pathological analysis, and ASM were dissected for Hsp70 detection. Normalized Hsp70 in ASM from COPD patients was significantly lower than that from controls (P <.001), and it was a significant positive correlation of Hsp70 and lung function. One-month exposure of rats to cigarette smoke/air mixture led to increased expression of Hsp70 and heat shock transcription factor (Hsf1) in ASM as compared to controls, whereas 3-month exposure caused dramatically reduced Hsp70 and Hsf1 than control animals. In addition, 3-month exposure to cigarette smoke/air mixture resulted in significantly lower Hsp70 and Hsf1 in rats ASM than 1-month exposure (P <.001), and it was a positive correlation of Hsf1 and Hsp70. Long-term cigarette smoking results in reduced expression of Hsp70 in ASM. This finding provides additional insight in understanding molecular changes in ASM during COPD pathogenesis.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Xie J,Zhao J,Xiao C,Xu Y,Yang S,Ni Wdoi
10.3109/01902140903349562subject
Has Abstractpub_date
2010-05-01 00:00:00pages
219-26issue
4eissn
0190-2148issn
1521-0499journal_volume
36pub_type
杂志文章abstract::The influence of gas composition on convective and diffusive gas transport in the lungs was assessed by studying the dispersion of combined particle and argon (Ar) boluses induced by the passage through the lungs filled with three different gas mixtures. Particles, as a "nondiffusing gas," served as a tracer for conve...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031767
更新日期:1995-11-01 00:00:00
abstract::The diabetic pregnancy is characterized by a delay in the process of fetal lung maturation. To determine whether high glucose conditions per se have an adverse impact on fetal lung phospholipid metabolism, 20-day fetal rat lung explants were cultured in F-12 medium (10 mM glucose) to which glucose was added to achieve...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309064361
更新日期:1993-11-01 00:00:00
abstract::A cast of human tracheobronchial airways, which is complete to airways less than 0.1 cm in diameter, was employed for experimental studies of gas convective transport by high-frequency ventilation (HFV). The cast was ventilated with gases of different kinematic viscosity (He, air, and SF6) and tidal volumes of 10-60 m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
abstract::Biochemical characterization of human respiratory mucus has generally utilized expectorated specimens. In order to exclude extraneous contaminants in the analysis of airway glycoproteins, human airways were cultured and the mucous glycoprotein released into the supernatant analyzed. By incorporating 3H-labeled glucosa...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409069675
更新日期:1984-01-01 00:00:00
abstract::In vitro observations of fetal lung maturation are complicated by continued epithelial cell maturation in culture. Removal of trace quantities of cortisol from the culture system has been reported elsewhere by Torday to prevent epithelial cell differentiation, but also prevent cell growth. In this report we have devel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061503
更新日期:1983-07-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::The separate effects of positive end-expiratory pressure (PEEP) and intravascular volume administration on the histopathologic lung injury were not investigated in experimental lung injury previously. The authors hypothesized that high PEEP and a restrictive volume therapy would yield the best oxygenation and the leas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.514023
更新日期:2011-02-01 00:00:00
abstract::Retention in the extrathoracic airways, and clearance by nose blowing, of monodisperse indium-111-labeled polystyrene particles were followed for at least 2 days after inhalation by healthy volunteers. Nine volunteers inhaled 3-μm aerodynamic diameter particles while sitting at rest, whereas subgroups of 3 or 4 inhale...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.518301
更新日期:2011-03-01 00:00:00
abstract::Prostaglandins (PGs) synthesized by fetal and neonatal lungs play pivotal roles in pulmonary physiology, especially during the transition from uterine to independent life. One regulator of prostaglandin synthesis at this time may be oxygen. We examined the effects of 1% O2, 21% O2 and 50% O2 in 5% CO2, balance N2 (PO2...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064301
更新日期:1987-01-01 00:00:00
abstract::Basement membranes (BM) are specialized extracellular matrices (ECM) which serve as complex interfaces between epithelia, peripheral nerves, or muscle cells and their surrounding tissue microenvironments. Their composition is known to include type IV collagen, laminin, entactin, heparan sulfate proteoglycan (HSPG, per...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149709074023
更新日期:1997-03-01 00:00:00
abstract::It is evident that much remains to be learned about the nasal passages and their responses to toxic materials. For the nose of both laboratory animals and humans, information is needed in the areas of anatomy, physiology, biochemistry, neurobiology, physiopathology, and oncology. This article briefly discussed toxic a...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149109064322
更新日期:1991-09-01 00:00:00
abstract::In a recent study of the sheep tracheal lobe model, we have demonstrated that surface chemistry modification of quartz by aluminum lactate significantly alters the biological activity of quartz for at least 2 months after exposure. In the present study, we have extended our observations of the biological reaction of t...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064319
更新日期:1987-01-01 00:00:00
abstract::Previous studies have indicated increased plasma levels of inducible nitric oxide synthase in lung. This study further examines the pulmonary expression of nitric oxide synthase (NOS) isoforms in an ovine model of acute lung injury induced by smoke inhalation and burn injury (S+B injury). Female range bred sheep (4 pe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802446832
更新日期:2009-03-01 00:00:00
abstract::The Ca(2+)-mobilizing actions of adenosine 5'-triphosphate (ATP), bradykinin, and histamine were compared in phenotypically distinct human nasal epithelial (HNE) cell types and as a function of time in cell culture. Single-cell measurements of intracellular free Ca2+ (Ca2+i, Fura-2 fluorescence) were recorded in cilia...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270196
更新日期:1999-06-01 00:00:00
abstract::Eprazinone therapy improves pulmonary function and arterial pO2 in patients with chronic bronchitis; however, the mechanism of action is unknown. The purpose of this study was to determine if eprazinone alters either lung surfactant levels in bronchoalveolar lavage fluid (BAL) of normal rats, or ion transport across c...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031693
更新日期:1992-05-01 00:00:00
abstract::Sirius Red staining is presented as a method for collagen determination, enabling quantitative morphometric measurements to be performed in locally defined tissue areas. The advantage of this method is especially shown for alveolar lung tissue. By excluding the bronchial areas in the tissue sections, the differences i...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031745
更新日期:1995-01-01 00:00:00
abstract::The aim of the study was to identify the effects on the lung after subchronic glass fiber intratracheal instillation study on rats. We evaluated the toxicological effects on the lung: persistent inflammatory reaction, cell proliferation, and pulmonary fibrosis on histopathological examination. We performed a glass fib...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.860568
更新日期:2014-03-01 00:00:00
abstract::Direct measurements of pleural fluid hydrostatic and colloid osmotic pressures after infusion of saline, bovine serum albumin, or silicone into the pleural space were coupled with ultrastructural morphometric analyses to assess the response of pleural mesothelial cells to hydrothorax. Increases of hydrostatic pressure...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309064347
更新日期:1993-05-01 00:00:00
abstract::The authors recently showed that preterm birth per se, in the absence of assisted ventilation or elevated inhaled oxygen levels, alters the structure of the airway walls in young lambs. The initial aim of the present study was to determine whether these changes persist into adulthood. Preterm (P; n = 7) lambs were del...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701807720
更新日期:2008-02-01 00:00:00
abstract::Expression of microRNAs (miRNAs) is characteristically altered in cancer, and they may play a role in cancer development and progression. The authors performed microarray and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses to determine the miRNA expression profile of primary sm...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902822312
更新日期:2009-10-01 00:00:00
abstract::Freeze-fracture was used to study the nonciliated epithelial (Clara) cell of rat bronchiole. En face views of the P face of the luminal cell membrane reveal particle-free bulging and circular depressions at the presumptive sites of the membrane fusion between the cell membrane and secretory granule membrane. Cross fra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052313
更新日期:1981-08-01 00:00:00
abstract:BACKGROUND:This study aims to explore the effect of thymoquinone (TQ) on particulate matter 2.5 (PM2.5)-induced lung injury. METHODS:The PM2.5 sample was provided by Shenyang Environment Monitor Central Station. Lung injury was established by intratracheal instillation PM2.5 (7.5 mg/kg) followed by TQ treatment (20 an...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1801895
更新日期:2020-10-01 00:00:00
abstract:PURPOSE:As an angiotensin converting enzyme (ACE) inhibitor, the effects of captopril on inflammation has been previously examined. Captopril has been shown to have anti-inflammatory and antioxidant effects. Imbalance in the oxidant/antioxidant system is one of the major causes of inflammation. In the present study, th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1473530
更新日期:2018-01-01 00:00:00
abstract::Intraphagosomal pH in rabbit alveolar macrophages was studied using amorphous silica particles (FSP) and yeast particles (FYP) labeled with fluorescein. The pH was estimated from the quotient between the fluorescence intensity at wavelength 519 nm with excitation at wavelengths 495 and 450 nm. Within a factor of 10, p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069608
更新日期:1989-01-01 00:00:00
abstract::Sex-related differences may be present during fetal lung growth and at the onset of surfactant synthesis. In this study we investigated the role of dihydrotestosterone (DHT) and estrogen (EST) on cell division and on labeled palmitate incorporation into disaturated phosphatidylcholine (DSPC) at various times of gestat...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087851
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:Mechanisms linking behavioral stress and inflammation are poorly understood, mainly in distal lung tissue. OBJECTIVE:We have investigated whether the forced swim stress (FS) could modulate lung tissue mechanics, iNOS, cytokines, oxidative stress activation, eosinophilic recruitment, and remodeling in guinea...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.704484
更新日期:2012-09-01 00:00:00
abstract:PURPOSE OF THE STUDY:Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and trea...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1505976
更新日期:2018-08-01 00:00:00
abstract::The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701703333
更新日期:2007-10-01 00:00:00
abstract::Amiodarone, a cardiac antiarrhythmic agent, has been associated with the development of interstitial pulmonary fibrosis in patients receiving prolonged therapy with the drug. To further assess the toxic effects of amiodarone on lung tissue, Syrian hamsters were given a single intratracheal insufflation of the agent an...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087891
更新日期:1984-01-01 00:00:00
abstract::The effects of activated leukocytes on surfactant function and composition were examined to better define the mechanisms by which acute inflammation contributes to respiratory distress syndrome (ARDS). Peripheral blood leukocytes from healthy volunteers were incubated in vitro with surfactant for 4 hours: (1) in the a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270042
更新日期:1999-10-01 00:00:00