Abstract:
:The effects of activated leukocytes on surfactant function and composition were examined to better define the mechanisms by which acute inflammation contributes to respiratory distress syndrome (ARDS). Peripheral blood leukocytes from healthy volunteers were incubated in vitro with surfactant for 4 hours: (1) in the absence of activation; (2) following activation by addition of phorbol myristate acetate (PMA); (3) following addition of PMA + FeCl2/EDTA; (4) PMA + FeCl2; (5) PMA + EDTA; (6) PMA + FeCl2 + superoxide dismutase (SOD) + catalase; (7) PMA + EDTA + serine or cysteine protease inhibitors. Surfactant was then repurified by sucrose gradient centrifugation, and function, phospholipid and protein composition, and extent of lipid peroxidation were assessed. PMA caused activation of leukocytes as detected by dichlorofluorescene assay. Lipid peroxidation (assessed by conjugated diene assay) was detected in all samples containing PMA. Abnormal function was noted in surfactant exposed to activated cells with 1 mM EDTA, whereas activation alone, or with FeCl2, had no effect. SOD + catalase prevented lipid peroxidation, but did not prevent leukocyte-mediated dysfunction, which was associated with a marked reduction in surfactant protein A (SP-A), but no detectable change in surfactant protein B (SP-B) or phospholipid composition. Serine protease inhibitors prevented SP-A degradation and dysfunction, whereas cysteine protease inhibitors had no protective effect. Addition of purified SP-A (5% w/w) to dysfunctional surfactant restored normal function, while SP-B/C mixture (3%) did not. Activated leukocytes cause surfactant dysfunction in vitro by serine protease-mediated degradation of SP-A, which occurs only in the presence of EDTA, and is prevented by addition of calcium. Although lipid peroxidation mediated by leukocyte release of free-radical products was also detected, surfactant dysfunction appears to be unrelated to this process.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Schochett P,Mora R,Mark L,Butler M,Ingenito EPdoi
10.1080/019021499270042subject
Has Abstractpub_date
1999-10-01 00:00:00pages
595-616issue
7eissn
0190-2148issn
1521-0499journal_volume
25pub_type
杂志文章abstract::Chronic obstructive pulmonary disease (COPD) as a complex disease with genetic and environmental compound is one of the leading causes of death in worldwide. This disease is characterized by lower airway inflammation, and increases risk of lung cancer in smokers. Micro-RNA (miRNA) molecules are key regulators in gene ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.689088
更新日期:2012-08-01 00:00:00
abstract::In vitro observations of fetal lung maturation are complicated by continued epithelial cell maturation in culture. Removal of trace quantities of cortisol from the culture system has been reported elsewhere by Torday to prevent epithelial cell differentiation, but also prevent cell growth. In this report we have devel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061503
更新日期:1983-07-01 00:00:00
abstract::The inhalation of inorganic dust can lead to the development of interstitial pulmonary fibrosis, characterized by the accumulation of fibroblasts and connective tissue matrix in the lung interstitium. The fibrosis causes alterations in the architecture of the lung parenchyma, resulting in abnormal gas exchange and hyp...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064332
更新日期:1991-11-01 00:00:00
abstract::Chlorinated amines are long-lived oxidants released from inflammatory cells. They have been observed to alter the function of important inflammatory molecules. Whether they can contribute directly to the inflammatory alteration of cellular barriers is not known. Monolayers of cultured porcine pulmonary artery endothel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087864
更新日期:1989-05-01 00:00:00
abstract::We have previously shown that intratracheally instilled silica (quartz) produces both morphologic evidence of emphysema and small-airway changes, and functional evidence of airflow obstruction. To further define the nature of the airflow-associated lesions induced by silica, we compared silica-exposed rats to rats giv...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069628
更新日期:1989-12-01 00:00:00
abstract::We set out to determine whether or not the "mucolytic" drug N-acetylcysteine would speed the reversal of cigarette smoke-induced secretory-cell hyperplasia to normal, similar to that found previously for two nonsteroidal antiinflammatory drugs. Cigarette smoke alone significantly (p less than 0.01) increased the numbe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148809062848
更新日期:1988-01-01 00:00:00
abstract::It is evident that much remains to be learned about the nasal passages and their responses to toxic materials. For the nose of both laboratory animals and humans, information is needed in the areas of anatomy, physiology, biochemistry, neurobiology, physiopathology, and oncology. This article briefly discussed toxic a...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149109064322
更新日期:1991-09-01 00:00:00
abstract::Intraphagosomal pH in rabbit alveolar macrophages was studied using amorphous silica particles (FSP) and yeast particles (FYP) labeled with fluorescein. The pH was estimated from the quotient between the fluorescence intensity at wavelength 519 nm with excitation at wavelengths 495 and 450 nm. Within a factor of 10, p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069608
更新日期:1989-01-01 00:00:00
abstract::Acute lung injury (ALI) is often associated with sepsis and is the most common cause of acute respiratory failure. The authors evaluated the role of the heme oxygenase (HO)/carbon monoxide (CO) system on lung injury in a cecal ligation and puncture (CLP)-induced mouse model of ALI. The authors established CLP-induced ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.660559
更新日期:2012-05-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::B6C3F1 female mice were exposed to cigarette smoke (CS) (250 mg/m3 total particulate material) or filtered air (FA), 6 hours/day, 5 days/week, for 6, 7, or 10 weeks, or to CS for 6 weeks, then FA for 1 or 4 additional weeks. Exposure to CS increased macrophages, neutrophils, lymphocytes, and matrix metalloproteinase (...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490252858
更新日期:2004-01-01 00:00:00
abstract::A number of novel chemotactic cytokines are becoming increasingly recognized as important participants in the elicitation of specific inflammatory cells from the peripheral blood to sites of inflammation. Recent observations have now demonstrated that certain chemotactic cytokines possess specificity for the selected ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109063278
更新日期:1991-01-01 00:00:00
abstract::This study evaluated polymorphonuclear leukocyte (PMN) involvement in translocation of dust to bronchial lymph nodes after deposition of dust in the lungs of control and neutropenic F344/N rats. Rats were rendered neutropenic with an intraperitoneal (IP) injection of anti-rat PMN antiserum (APA); control rats were inj...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149709046048
更新日期:1997-01-01 00:00:00
abstract::Purpose: Mechanical ventilation (MV) is an essential life support tool for patients with acute respiratory distress syndrome (ARDS). However, MV for ARDS can result in ventilator-induced lung injury (VILI). This study aimed to assess whether alpha 1-antitrypsin (AAT) can reduce VILI in ARDS rats. Materials and Methods...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2019.1642968
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:This study aimed to investigate differential cell responses of alveolar epithelial cells (AECs) after treatments with lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) to mimic the exposure to inflammation and oxidative stress and the mechanisms of a double-hit model of apoptosis. MATERIALS AND METHODS:AEC...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1368738
更新日期:2017-10-01 00:00:00
abstract::The cigarette smoke-induced rat model of chronic bronchitis was used to study the time course of the return of cigarette smoke-induced secretory cell hyperplasia to the normal and the capacity of two non-steroidal anti-inflammatory drugs to speed this recovery. Cigarette smoke alone significantly increased (P less tha...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148609061499
更新日期:1986-01-01 00:00:00
abstract::Activity for nonspecific esterases has been demonstrated histochemically in fetal rabbit lungs using alpha-naphthyl acetate as substrate. Commencing with a homogeneous distribution in pseudoglandular lungs, activity gradually heightens in the epithelium, progresses centrifugally along the bronchial tree, and reaches f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209069657
更新日期:1982-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Serum amyloid A (SAA), an acute-phase protein whose level tracks infection and inflammation, is the precursor protein of amyloid A (AA) fibrils that is thought to cause AA amyloidosis in human and animals. SAA protein has several isoforms based on the difference of amino acid sequence, such as SA...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1809750
更新日期:2020-11-01 00:00:00
abstract::The effect of glucocorticoids on the response of adenylate cyclase in fetal rat lung fibroblast and Type II epithelial cell cultures to beta-agonists and prostaglandin E2 (PGE2) was investigated. There was significant stimulation of cyclic AMP synthesis by adrenalin in both fibroblasts and Type II cells, which was pot...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087863
更新日期:1989-05-01 00:00:00
abstract:AIM OF THE STUDY:Interleukin (IL)-10 is an anti-inflammatory cytokine, but its role in cigarette smoke (CS)-induced inflammation and chronic obstructive pulmonary disease (COPD) has not been fully elucidated. The purpose of this study was to investigate the effect of IL-10 deficiency on CS-induced pulmonary inflammatio...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1096315
更新日期:2015-01-01 00:00:00
abstract::A cast of human tracheobronchial airways, which is complete to airways less than 0.1 cm in diameter, was employed for experimental studies of gas convective transport by high-frequency ventilation (HFV). The cast was ventilated with gases of different kinematic viscosity (He, air, and SF6) and tidal volumes of 10-60 m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
abstract:PURPOSE:This study was prospectively designed to investigate the effects of different concentrations of isoflurane (ISO) pretreatment on respiratory mechanics, oxygenation, and hemodynamics in lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) model of juvenile piglets. METHODS:Twenty-four pig...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1054530
更新日期:2015-01-01 00:00:00
abstract::Prostaglandins (PGs) synthesized by fetal and neonatal lungs play pivotal roles in pulmonary physiology, especially during the transition from uterine to independent life. One regulator of prostaglandin synthesis at this time may be oxygen. We examined the effects of 1% O2, 21% O2 and 50% O2 in 5% CO2, balance N2 (PO2...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064301
更新日期:1987-01-01 00:00:00
abstract::The influence of gas composition on convective and diffusive gas transport in the lungs was assessed by studying the dispersion of combined particle and argon (Ar) boluses induced by the passage through the lungs filled with three different gas mixtures. Particles, as a "nondiffusing gas," served as a tracer for conve...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031767
更新日期:1995-11-01 00:00:00
abstract::The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701539745
更新日期:2007-08-01 00:00:00
abstract::The aim of this study was to determine influence of prenatal granulocyte-macrophage colony-stimulating factor (GM-CSF) administration on lung growth, maturation, and vascular endothelial growth factor (VEGF) expression. Twenty Wistar rats received sterile saline (1 mL) or recombinant human GM-CSF (50 micro g/kg) on da...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802341736
更新日期:2008-11-01 00:00:00
abstract::The production of reactive oxygen species (ROS) during hyperoxia contribute to alveolar epithelial apoptosis. In the present study, the molecular mechanisms of oxidative stress-induced alveolar epithelial cell apoptosis were investigated. The cytoprotective effects of N-acetylcysteine (NAC) were evaluated. Treatments ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902141003678582
更新日期:2010-08-01 00:00:00
abstract::We studied lung clearance of aerosolized technetium-labeled diethylenetriamine pentaacetic acid (99mTcDTPA), plasma concentrations of 6-keto-PGF1 alpha and thromboxane B2, and pulmonary edema as indices of lung injury in rabbits exposed to cigarette smoke (CSE). Forty-six rabbits were randomly assigned to 4 groups: co...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148709064313
更新日期:1987-01-01 00:00:00
abstract::It has been suggested that increased recruitment of phagocytes and subsequent clearance of particles may follow instillation of a leukocyte chemoattractant to lungs containing silica. The present study quantitated serially the silica content in alveolar spaces, in lung tissue and in hilar lymph nodes of mice that rece...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149409064384
更新日期:1994-05-01 00:00:00
abstract::Inflammation plays a key role in lung injury and in the pathogenesis of asthma. Two murine models of allergic airway inflammation-sensitization and challenge to ovalbumin (OVA) and intratracheal exposure to interleukin-13 (IL13)-were used to evaluate the expression of poly(ADP-ribose) polymerase-1 (PARP-1) in allergic...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902141003663360
更新日期:2010-09-01 00:00:00