Abstract:
:Both hyperoxia-induced proapoptotic sensitization of alveolar type II cells (TII cells) and high-stretch mechanical ventilation induced pulmonary inflammation are tumor necrosis factor alpha (TNFalpha) mediated. Therefore, binding of free TNFalpha should protect from TNFalpha-mediated acute lung injury and ameliorate the subsequently developing chronic lung disease. Here, the authors show that a single subcutaneous pretreatment of rat with etanercept, a recombinant p75 TNF receptor 2 human immunoglobulin G1 (IgG1) construct, inhibits the hyperoxia-induced and TNFalpha-mediated increase in the expression of TNFalpha receptor, the activation of caspase 3 in TII cells, and, as an early indicator of lung injury, the capillary-alveolar leakage and granulocyte number in lung lavage. The authors assume that subcutaneous administration of etanercept might be suitable to prevent acute lung injury and its sequelae induced by hyperoxic ventilation of premature neonates and critically ill patients.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Guthmann F,Wissel H,Rüstow Bdoi
10.3109/01902140902887430subject
Has Abstractpub_date
2009-11-01 00:00:00pages
770-80issue
9eissn
0190-2148issn
1521-0499pii
10.3109/01902140902887430journal_volume
35pub_type
杂志文章abstract:BACKGROUND:Asthma is a chronic inflammatory disease of the airway that is mediated by T-helper 2(TH2) cells. Thymic stromal lymphopoietin (TSLP) can aggravate asthmatic lung inflammation by activating dendritic cells (DCs) to promote TH2 differentiation. TSLP promoter polymorphisms are associated with susceptibility to...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.714840
更新日期:2012-10-01 00:00:00
abstract:AIM OF THE STUDY:Interleukin (IL)-10 is an anti-inflammatory cytokine, but its role in cigarette smoke (CS)-induced inflammation and chronic obstructive pulmonary disease (COPD) has not been fully elucidated. The purpose of this study was to investigate the effect of IL-10 deficiency on CS-induced pulmonary inflammatio...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2015.1096315
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:This study aims to explore the effect of thymoquinone (TQ) on particulate matter 2.5 (PM2.5)-induced lung injury. METHODS:The PM2.5 sample was provided by Shenyang Environment Monitor Central Station. Lung injury was established by intratracheal instillation PM2.5 (7.5 mg/kg) followed by TQ treatment (20 an...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1801895
更新日期:2020-10-01 00:00:00
abstract::Intratracheal bleomycin induces pulmonary fibrosis in experimental animals, but the mechanisms involved are poorly understood. Since altered levels of fatty acid metabolites are associated with bleomycin-induced lung injury, we examined the effects of a change in dietary fat on bleomycin-induced fibrosis. Previously w...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087861
更新日期:1989-03-01 00:00:00
abstract::The influence of gas composition on convective and diffusive gas transport in the lungs was assessed by studying the dispersion of combined particle and argon (Ar) boluses induced by the passage through the lungs filled with three different gas mixtures. Particles, as a "nondiffusing gas," served as a tracer for conve...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509031767
更新日期:1995-11-01 00:00:00
abstract::Inflammation plays a key role in lung injury and in the pathogenesis of asthma. Two murine models of allergic airway inflammation-sensitization and challenge to ovalbumin (OVA) and intratracheal exposure to interleukin-13 (IL13)-were used to evaluate the expression of poly(ADP-ribose) polymerase-1 (PARP-1) in allergic...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902141003663360
更新日期:2010-09-01 00:00:00
abstract::It was hypothesized that IL-8, a neutrophil chemotaxin, contributes to the influx of neutrophils into the pleural cavity of patients with pleural effusions. Pleural fluids were collected from 57 patients including 13 with effusions due to congestive heart failure, 28 with pleural involvement by carcinoma, 5 with empye...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309031730
更新日期:1993-09-01 00:00:00
abstract::Increase in intracellular adenosine 3', 5'-cyclic monophosphate (cAMP) is a common pathway for many clinically used drugs to cause pulmonary artery (PA) relaxation. Activity of sarcolemmal K+ and Cl(-)-channels is an important determinant of membrane potential (Em), which, in turn, plays a critical role in regulating ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809046055
更新日期:1998-01-01 00:00:00
abstract::Previously, this laboratory has shown that intratracheally administered hyaluronic acid (HA) significantly reduces air-space enlargement in a hamster model of emphysema induced with pancreatic elastase. Whereas HA was given immediately following elastase in those initial studies, the current investigation determined t...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149709087369
更新日期:1997-05-01 00:00:00
abstract::Chlorinated amines are long-lived oxidants released from inflammatory cells. They have been observed to alter the function of important inflammatory molecules. Whether they can contribute directly to the inflammatory alteration of cellular barriers is not known. Monolayers of cultured porcine pulmonary artery endothel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087864
更新日期:1989-05-01 00:00:00
abstract::Understanding the effects from long-term exposure to individual ambient air pollutants and mixtures of pollutants is necessary for adequate assessment of health risk. This study examined quantitative and temporal alterations in tracheobronchial mucociliary clearance function and bronchial epithelial secretory cells in...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209064343
更新日期:1992-07-01 00:00:00
abstract::Aberrant expression of key cell cycle regulatory genes is essential for the immortalization and transformation of cells in vitro. We examined 20 mouse lung epithelial cell lines (2 nontumorigenic, 5 nonmetastatic, and 13 metastatic) for mutations or alterations in the expression of key components of the Rb pathway (pR...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401300054064
更新日期:2001-04-01 00:00:00
abstract::Protein tyrosine phosphatase is an important class of enzymes that plays an essential role in the cellular proliferation, differentiation, and oncogenesis. In this paper we report characterization of a low-molecular-weight protein tyrosine phosphatase purified from bovine lung. The enzyme activity was essentially inde...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809041534
更新日期:1998-05-01 00:00:00
abstract::A cast of human tracheobronchial airways, which is complete to airways less than 0.1 cm in diameter, was employed for experimental studies of gas convective transport by high-frequency ventilation (HFV). The cast was ventilated with gases of different kinematic viscosity (He, air, and SF6) and tidal volumes of 10-60 m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
abstract::Intraphagosomal pH in rabbit alveolar macrophages was studied using amorphous silica particles (FSP) and yeast particles (FYP) labeled with fluorescein. The pH was estimated from the quotient between the fluorescence intensity at wavelength 519 nm with excitation at wavelengths 495 and 450 nm. Within a factor of 10, p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069608
更新日期:1989-01-01 00:00:00
abstract::CTP:cholinephosphate cytidylyltransferase (CT) is a key enzyme required for surfactant phosphatidylcholine synthesis, and its activity is regulated by lung lipids. This study evaluated the effect of epidermal growth factor (EGF) on the phospholipid content and the expression of CT in the lung following direct in vivo ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149409064369
更新日期:1994-01-01 00:00:00
abstract::The role of type II epithelial cell proliferation in repair of diffuse alveolar epithelial injury was examined using two pneumotoxicants, 3-methylindole (3-MI) and monocrotaline (MCT). It was hypothesized that if MCT inhibits type II epithelial cell mitosis, then pulmonary fibrosis would result after diffuse 3-MI-indu...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270376
更新日期:1999-03-01 00:00:00
abstract::Long-term, low-dose macrolide therapy has been proven to improve survival in patients with diffuse panbronchiolitis and cystic fibrosis, although the mechanisms by which it does so remain unknown. To elucidate the molecular mechanisms of the anti-inflammatory effects of macrolides, the authors examined the effects of ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802093238
更新日期:2008-08-01 00:00:00
abstract::Distinct barrier lesions and an apical-basal distribution of alveolar edema fluid in either moderate or high elevated pressure edema lungs have been found in previous studies. In the present study, quantitative measurements were obtained by using electron microscopy and morphometry of extravascular lung water and barr...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149509050838
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Inhaled capsaicin (8-methyl-N-vanillyl-6-nonenamide) has been used to induce cough in a safe and dose-dependent manner. Chronic cough is associated with an increased sensitivity to inhaled capsaicin in patients with asthma. The aim of this study was to evaluate clinical impact of capsaicin provocation test f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1834016
更新日期:2021-02-01 00:00:00
abstract::We investigated the distribution of adrenergic receptors in ferret trachea using autoradiography. [3H]Dihydroalprenolol, used to identify beta-adrenoceptors, revealed a high density of specific binding sites over surface epithelium and submucosal glands, with less labelling of smooth muscle. [3H]prazosin labelling sho...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148309061513
更新日期:1983-11-01 00:00:00
abstract::The studies presented were designed to test the efficacy of farnesyltransferase inhibitors (FTIs) as potential chemopreventive compounds in the mouse lung tumor model, and in tumor cell lines. The compounds included manumycin, gliotoxin, dihydroepiandrosterone (DHEA), perillyl alcohol (POH), and FTI-276. Each of these...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140150216819
更新日期:2000-12-01 00:00:00
abstract::The effects of activated leukocytes on surfactant function and composition were examined to better define the mechanisms by which acute inflammation contributes to respiratory distress syndrome (ARDS). Peripheral blood leukocytes from healthy volunteers were incubated in vitro with surfactant for 4 hours: (1) in the a...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270042
更新日期:1999-10-01 00:00:00
abstract::Transforming growth factor alpha (TGF(alpha)) stimulates type II alveolar epithelial cell proliferation and also is associated with fibrosis. We studied the changes in bronchoalveolar lavage (BAL) TGF(alpha) protein in a neonatal rabbit hyperoxia-fibrosis model (100% O(2) for 8 to 9 days, followed by 60% O(2) to 36 da...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290091994
更新日期:2002-07-01 00:00:00
abstract::This report describes the protocol for the strain A mouse lung tumor bioassay and summarizes results on selected chemicals that have been tested for carcinogenicity in the assay. The assay is of 6 months duration and can distinguish 2-fold differences in carcinogenic potential of compounds from several chemical classe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064428
更新日期:1991-03-01 00:00:00
abstract:AIM OF THE STUDY:Long-term exposure to cigarette smoke generates chronic obstructive pulmonary disease (COPD) in guinea pigs, but a comprehensive evaluation of changes in lung function, as assessed by barometric whole body plethysmography (WBP), is lacking. MATERIALS AND METHODS:Female guinea pigs were exposed to the ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1294632
更新日期:2017-03-01 00:00:00
abstract::The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701539745
更新日期:2007-08-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::Studies demonstrated that pathophysiological abnormalities of airway smooth muscle (ASM) contribute significantly to chronic obstructive pulmonary disease (COPD) pathogenesis, the aim of this study is to investigate heat shock protein 70 (Hsp70) in ASM in COPD. ASM from 8 COPD patients and 6 controls were isolated for...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140903349562
更新日期:2010-05-01 00:00:00
abstract::Expression of microRNAs (miRNAs) is characteristically altered in cancer, and they may play a role in cancer development and progression. The authors performed microarray and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses to determine the miRNA expression profile of primary sm...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902822312
更新日期:2009-10-01 00:00:00