Abstract:
:Long-term, low-dose macrolide therapy has been proven to improve survival in patients with diffuse panbronchiolitis and cystic fibrosis, although the mechanisms by which it does so remain unknown. To elucidate the molecular mechanisms of the anti-inflammatory effects of macrolides, the authors examined the effects of erythromycin (EM-A) and new derivative EM703 on transforming growth factor (TGF)-beta /Smad signaling fibroblasts. EM-A and EM703 each inhibited fibroblast proliferation and the collagen production in human lung fibroblasts induced by TGF-beta. EM-A and EM703 inhibited the augmentation of Smad3 mRNA induced by TGF-beta. Smad7 mRNA was inhibited by TGF-beta, but augmented by coincubation with EM-A or EM703. EM-A and EM703 each inhibited p-Smad2/3 proteins induced by TGF-beta. Smad7 protein inhibited by TGF-beta restored beyond basal level by EM-A and EM703. These findings suggest that EM-A and EM703 inhibit TGF-beta signaling in human lung fibroblasts via inhibition of p-Smad2/3 through recovery of Smad7 level.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Yu C,Azuma A,Li Y,Wang C,Abe S,Usuki J,Matsuda K,Kudoh S,Sunazuka T,Omura Sdoi
10.1080/01902140802093238subject
Has Abstractpub_date
2008-08-01 00:00:00pages
343-54issue
6eissn
0190-2148issn
1521-0499pii
794713819journal_volume
34pub_type
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