Abstract:
:Neutrophilic inflammation is responsible for much of the tissue damage seen in many lung diseases. For resolution of inflammation to occur, neutrophils must die by apoptosis, allowing their recognition and removal by macrophages. The molecular events controlling this important regulatory step are poorly understood, in large part due to the genetic intractability of the human neutrophil granulocyte. The authors have established a model of inflammation in the Zebrafish, which shares many features of the innate immune system with those of humans. Injury to the Zebrafish tailfin induces a reproducible and quantifiable inflammatory response, which resolves with kinetics similar to mammalian models of neutrophilic inflammation, including pulmonary inflammation. Pharmacological modulation of neutrophil apoptosis can modulate the outcome of experimentally induced inflammation. In addition, the authors have generated a construct that expresses green fluorescent protein under the myeloperoxidase promoter, allowing in vivo visualization of neutrophils during experimentally induced inflammation. The authors are also performing an unbiased forward genetic screen for mutants with defective resolution of inflammation, and to date have identified a number of putative mutants. Further study and characterization of these mutants is underway. The authors have thus established an important experimental link between apoptosis and resolution of inflammation in an in vivo system, and defined an important new model for the study of inflammation resolution. The authors hope that these tools will permit detailed study of the genetic controls of the resolution of inflammation, and provide insights with potential clinical utility.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Renshaw SA,Loynes CA,Elworthy S,Ingham PW,Whyte MKdoi
10.1080/01902140701756778subject
Has Abstractpub_date
2007-12-01 00:00:00pages
549-54issue
10eissn
0190-2148issn
1521-0499pii
788420592journal_volume
33pub_type
杂志文章,评审abstract::The effects of Zizyphi fructus, a major constituent of Chinese anti-asthmatic herbal medicine, on ciliary motility and nitric oxide (NO) generation in canine cultured tracheal epithelium were studied by the microphoto-oscillation method and the specific amperometric method, respectively. Addition of Zizyphi fructus at...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149609031774
更新日期:1996-05-01 00:00:00
abstract::We compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. Judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. Lung volumes in both air- and saline-filled lungs and mean chord length (Lm) were incre...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069638
更新日期:1989-12-01 00:00:00
abstract::We have previously shown that intratracheally instilled silica (quartz) produces both morphologic evidence of emphysema and small-airway changes, and functional evidence of airflow obstruction. To further define the nature of the airflow-associated lesions induced by silica, we compared silica-exposed rats to rats giv...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069628
更新日期:1989-12-01 00:00:00
abstract::Leukotrienes (LTs) are potent vasoconstrictors in the pulmonary circulation. We investigated LTB4 and LTE4 metabolism by intrapulmonary arteries and veins of 2 to 9 days old lambs (n = 6). Paired vessels were incubated under baseline, and stimulated conditions. LTB4 and LTE4 were extracted from media, quantfied by enz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401750193601
更新日期:2001-06-01 00:00:00
abstract::The objective of this study was to assess the radioprotective effects of amifostine in the rat model of radiation-induced lung injury using fractionated doses of radiation, to determine whether amifostine given before irradiation protects tumor from radiation cytotoxicity, and to determine whether changes in plasma le...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290096791
更新日期:2002-10-01 00:00:00
abstract:PURPOSE:As an angiotensin converting enzyme (ACE) inhibitor, the effects of captopril on inflammation has been previously examined. Captopril has been shown to have anti-inflammatory and antioxidant effects. Imbalance in the oxidant/antioxidant system is one of the major causes of inflammation. In the present study, th...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1473530
更新日期:2018-01-01 00:00:00
abstract::Hypercholesterolemia contributes to cardiovascular diseases, but its direct effect on lung is little known. 3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) have been shown to exert numerous effects that are dependent and independent of their cholesterol-lowering property. The authors hyp...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902141003739715
更新日期:2010-12-01 00:00:00
abstract::Expression of microRNAs (miRNAs) is characteristically altered in cancer, and they may play a role in cancer development and progression. The authors performed microarray and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses to determine the miRNA expression profile of primary sm...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902140902822312
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1451574
更新日期:2018-03-01 00:00:00
abstract::Basement membranes (BM) are specialized extracellular matrices (ECM) which serve as complex interfaces between epithelia, peripheral nerves, or muscle cells and their surrounding tissue microenvironments. Their composition is known to include type IV collagen, laminin, entactin, heparan sulfate proteoglycan (HSPG, per...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149709074023
更新日期:1997-03-01 00:00:00
abstract::A cast of human tracheobronchial airways, which is complete to airways less than 0.1 cm in diameter, was employed for experimental studies of gas convective transport by high-frequency ventilation (HFV). The cast was ventilated with gases of different kinematic viscosity (He, air, and SF6) and tidal volumes of 10-60 m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209031698
更新日期:1992-09-01 00:00:00
abstract:PURPOSE OF THE STUDY:Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and trea...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2018.1505976
更新日期:2018-08-01 00:00:00
abstract::Direct measurements of pleural fluid hydrostatic and colloid osmotic pressures after infusion of saline, bovine serum albumin, or silicone into the pleural space were coupled with ultrastructural morphometric analyses to assess the response of pleural mesothelial cells to hydrothorax. Increases of hydrostatic pressure...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149309064347
更新日期:1993-05-01 00:00:00
abstract::This report describes the protocol for the strain A mouse lung tumor bioassay and summarizes results on selected chemicals that have been tested for carcinogenicity in the assay. The assay is of 6 months duration and can distinguish 2-fold differences in carcinogenic potential of compounds from several chemical classe...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149109064428
更新日期:1991-03-01 00:00:00
abstract::Immunosuppressive and antiproliferative effects of heparin may be beneficial in the field of solid organ transplantation. The aim of this study was to examine the effect of low-molecular-weight heparin (LMWH) compounds on the development of obliterative airway disease (OAD) in the rat tracheal transplant model. Alloge...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.499443
更新日期:2010-12-01 00:00:00
abstract::It has been suggested that increased recruitment of phagocytes and subsequent clearance of particles may follow instillation of a leukocyte chemoattractant to lungs containing silica. The present study quantitated serially the silica content in alveolar spaces, in lung tissue and in hilar lymph nodes of mice that rece...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149409064384
更新日期:1994-05-01 00:00:00
abstract::Retention in the extrathoracic airways, and clearance by nose blowing, of monodisperse indium-111-labeled polystyrene particles were followed for at least 2 days after inhalation by healthy volunteers. Nine volunteers inhaled 3-μm aerodynamic diameter particles while sitting at rest, whereas subgroups of 3 or 4 inhale...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2010.518301
更新日期:2011-03-01 00:00:00
abstract::Freeze-fracture was used to study the nonciliated epithelial (Clara) cell of rat bronchiole. En face views of the P face of the luminal cell membrane reveal particle-free bulging and circular depressions at the presumptive sites of the membrane fusion between the cell membrane and secretory granule membrane. Cross fra...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052313
更新日期:1981-08-01 00:00:00
abstract::Intratracheal bleomycin induces pulmonary fibrosis in experimental animals, but the mechanisms involved are poorly understood. Since altered levels of fatty acid metabolites are associated with bleomycin-induced lung injury, we examined the effects of a change in dietary fat on bleomycin-induced fibrosis. Previously w...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909087861
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:Inhaled capsaicin (8-methyl-N-vanillyl-6-nonenamide) has been used to induce cough in a safe and dose-dependent manner. Chronic cough is associated with an increased sensitivity to inhaled capsaicin in patients with asthma. The aim of this study was to evaluate clinical impact of capsaicin provocation test f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1834016
更新日期:2021-02-01 00:00:00
abstract::In vitro responses of potential target cell types to air pollutants under physiological conditions may be useful in understanding the health effects of air pollution exposure. The study evaluated responses of human primary airway epithelial cells to diesel exhaust (DE). Cultures of cells from 3 donors, differentiated ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140601113088
更新日期:2007-01-01 00:00:00
abstract::The role of type II epithelial cell proliferation in repair of diffuse alveolar epithelial injury was examined using two pneumotoxicants, 3-methylindole (3-MI) and monocrotaline (MCT). It was hypothesized that if MCT inhibits type II epithelial cell mitosis, then pulmonary fibrosis would result after diffuse 3-MI-indu...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270376
更新日期:1999-03-01 00:00:00
abstract::COPD remains a public health problem that has yet to be fully recognized by the public, the medical community or public health (government) officials, and much work remains to be done worldwide to reverse the increasing trends of this chronic lung disease. ...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:
更新日期:2005-09-01 00:00:00
abstract::Tracheal occlusion (TO) performed surgically in utero near the end of gestation causes a rapid increase in the distension of future airspaces, resulting in accelerated lung development. The authors hypothesize that TO stimulates microvascular growth concomitant with a rapid increase in the expression of genes implicat...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140701884331
更新日期:2008-03-01 00:00:00
abstract::Emerging data indicate that endothelial-mesenchymal transition (EndMT) is involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). A previous study noted that blocking the activity of protein phosphatase 2 A (PP2A) could attenuate EndMT. However, the treatment effects of PP2A inhibitors in pulmonary fibros...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1774823
更新日期:2020-09-01 00:00:00
abstract::Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar bodies consisted largel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209115812
更新日期:1982-02-01 00:00:00
abstract::The percentage 24-h lung retention of 4-micrometers monodispersed Teflon particles, aerodynamic diameter about 6 micrometers, was studied twice in 8 healthy nonsmokers. The particles were inhaled at 0.5 liter/sec with maximally deep breaths. Bronchoconstriction was induced by inhalation of a methacholine-bromide aeros...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087917
更新日期:1984-01-01 00:00:00
abstract::It is evident that much remains to be learned about the nasal passages and their responses to toxic materials. For the nose of both laboratory animals and humans, information is needed in the areas of anatomy, physiology, biochemistry, neurobiology, physiopathology, and oncology. This article briefly discussed toxic a...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149109064322
更新日期:1991-09-01 00:00:00
abstract::Previous experiments with the sheep model of silicosis have demonstrated a reduction of the pathological activity of quartz after treatments with aluminum lactate (Al-lac), such as Al-lac pretreatment of intratracheally injected quartz or Al-lac inhalation of quartz-exposed animals. Preliminary data also suggested a m...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149409064380
更新日期:1994-03-01 00:00:00
abstract::Pathophysiologic changes induced by phospholipase A2 (PLA2), a lipolytic enzyme implicated in a variety of pulmonary diseases, were examined in the isolated, perfused guinea pig lung. Thirty microliters of vehicle (saline) or 30 units of PLA2 suspended in saline was given as a bolus injection into either the trachea o...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149009108848
更新日期:1990-07-01 00:00:00