Abstract:
AIMS:As a transcription factor of the nuclear receptor superfamily, neuron-derived orphan receptor 1 (NOR1) is induced rapidly in response to various extracellular stimuli. But, it is still unclear its role in pulmonary artery smooth muscle cells proliferation. MATERIALS AND METHODS:Human PASMCs were cultured in vitro and stimulated by serum. The special antisense oligodeoxynucleotides (AS-ODNs) were used to knockdown human NOR1 gene expression. Real-time PCR and Western-blot were used to evaluate the gene expression and protein levels. RESULTS:Fetal bovine serum (FBS) induced human PASMCs proliferation in a dose dependent manner. Furthermore, FBS promoted NOR1 gene expression in a dose dependent manner and a time dependent manner. 10% FBS induced a maximal NOR1 mRNA levels at 2 h. FBS also induced a significant higher NOR1 protein levels as compared with control. The NOR1 over-expressed plasmid significantly promoted DNA synthesis and cells proliferation. Moreover, the special AS-ODNs against human NOR1 not only prevented NOR1 expression but also inhibited DNA synthesis and cells proliferation significantly. The NOR1 over-expression plasmid could up-regulate cyclin D1 expression markedly, but the AS-ODNs inhibited cyclin D1 expression significantly. CONCLUSION:So, we concluded that NOR1 could promote human PASMCs proliferation. Cyclin D1 might be involved in this process.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Wang CG,Lei W,Li C,Zeng DX,Huang JAdoi
10.3109/01902148.2014.993776subject
Has Abstractpub_date
2015-05-01 00:00:00pages
208-15issue
4eissn
0190-2148issn
1521-0499journal_volume
41pub_type
杂志文章abstract::Previously, this laboratory has shown that intratracheally administered hyaluronic acid (HA) significantly reduces air-space enlargement in a hamster model of emphysema induced with pancreatic elastase. Whereas HA was given immediately following elastase in those initial studies, the current investigation determined t...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149709087369
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
pub_type: 杂志文章,评审
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更新日期:1997-03-01 00:00:00
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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更新日期:2017-10-01 00:00:00
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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doi:10.3109/01902148.2010.520815
更新日期:2011-03-01 00:00:00
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journal_title:Experimental lung research
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doi:10.3109/01902149509031745
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journal_title:Experimental lung research
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069615
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journal_title:Experimental lung research
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journal_title:Experimental lung research
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pub_type: 杂志文章,评审
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journal_title:Experimental lung research
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doi:10.3109/01902148.2011.633306
更新日期:2012-02-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209039246
更新日期:1982-12-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140150216819
更新日期:2000-12-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
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更新日期:2020-12-30 00:00:00
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journal_title:Experimental lung research
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doi:10.1080/01902140490463142
更新日期:2004-07-01 00:00:00
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更新日期:2015-01-01 00:00:00
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journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087896
更新日期:1984-01-01 00:00:00
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journal_title:Experimental lung research
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更新日期:2016-09-01 00:00:00