Abstract:
:Irreversible lung parenchymal injury is usually healed by fibrosis, which depends on the abilities of fibroblasts to proliferate, migrate into the wound, and survive. Because the lung is frequently exposed to increased oxidative stress, which is thought to mediate apoptosis, we examined whether oxidative stress induces apoptosis in fibroblasts during wound healing. We performed an in vitro scratch wound assay where cultured fibroblast monolayers were exposed to H2O2 (10-500 microM) after artificial wounding. Apoptosis was evaluated by nuclear staining with Hoechst33342 or terminal deoxynucleotidyl transferase (TdT)-mediated nucleotide nick end-labeling (TUNEL). Intracellular oxidants were assessed with the peroxide-sensitive fluorochrome carboxydichlorodihydrofluorescein (CDCF). We found that repopulating fibroblasts at the wound margin, but not quiescent fibroblasts at the intact site, selectively underwent oxidant accumulation and apoptosis in response to H2O2 exposure. Some of the apoptotic cells had incorporated bromodeoxyuridine (BrdU), an indicator of proliferating cells. These results suggest that oxidative stress selectively induces apoptosis in fibroblasts that are stimulated to proliferate and/or migrate into the wound. Fibroblast apoptosis induced by oxidative stress during wound repopulation may be relevant to intractable wound healing.
journal_name
Exp Lung Resjournal_title
Experimental lung researchauthors
Takahashi A,Aoshiba K,Nagai Adoi
10.1080/01902140252964366subject
Has Abstractpub_date
2002-06-01 00:00:00pages
275-84issue
4eissn
0190-2148issn
1521-0499journal_volume
28pub_type
杂志文章abstract:PURPOSE:This study aimed to investigate differential cell responses of alveolar epithelial cells (AECs) after treatments with lipopolysaccharide (LPS) and hydrogen peroxide (H2O2) to mimic the exposure to inflammation and oxidative stress and the mechanisms of a double-hit model of apoptosis. MATERIALS AND METHODS:AEC...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2017.1368738
更新日期:2017-10-01 00:00:00
abstract::The molecular events involved in both the initiation and development of silicosis are at present poorly defined, although mediators released from macrophages exposed to silica particles are believed to play a role. We have investigated the in vitro production of arachidonic acid (AA) metabolites in adherent bovine alv...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148909069615
更新日期:1989-07-01 00:00:00
abstract::Previously, this laboratory has shown that intratracheally administered hyaluronic acid (HA) significantly reduces air-space enlargement in a hamster model of emphysema induced with pancreatic elastase. Whereas HA was given immediately following elastase in those initial studies, the current investigation determined t...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149709087369
更新日期:1997-05-01 00:00:00
abstract::Activity for nonspecific esterases has been demonstrated histochemically in fetal rabbit lungs using alpha-naphthyl acetate as substrate. Commencing with a homogeneous distribution in pseudoglandular lungs, activity gradually heightens in the epithelium, progresses centrifugally along the bronchial tree, and reaches f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209069657
更新日期:1982-11-01 00:00:00
abstract::Nuclear factor kappa B (NF-kappa B) is a critical transcription factor for the production of many inflammatory cytokines. It is activated in the airway epithelium of human asthmatics and in mice after allergic stimulation. To examine the role of NF-kappa B activation in allergic inflammation, the authors generated tra...
journal_title:Experimental lung research
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doi:10.3109/01902140903019710
更新日期:2009-12-01 00:00:00
abstract::Emerging data indicate that endothelial-mesenchymal transition (EndMT) is involved in the pathogenesis of idiopathic pulmonary fibrosis (IPF). A previous study noted that blocking the activity of protein phosphatase 2 A (PP2A) could attenuate EndMT. However, the treatment effects of PP2A inhibitors in pulmonary fibros...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2020.1774823
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Bronchopulmonary dysplasia (BPD) is a main chronic lung disease commonly occurs in preterm infants. BPD is characterized by impaired alveolarization and vascularization of the developing lung. Transforming growth factor-β (TGF-β) signaling pathway is known to play an important role during lung vascular devel...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2016.1226448
更新日期:2016-09-01 00:00:00
abstract::Two morphologic characteristics have been used to define the nonciliated bronchiolar epithelial cell: abundant agranular endoplasmic reticulum (AER) and membrane-bound avoid granules. To assess the ultrastructural homogeneity of this cell type in the lungs of large domestic mammals used as experimental models in pulmo...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148009069645
更新日期:1980-06-01 00:00:00
abstract::Investigators disagree on the amount of hydrogen peroxide (H2O2) released by resting and stimulated alveolar macrophages. The method commonly used to measure H2O2 release involves horseradish peroxidase (HRP)-catalyzed oxidation of scopoletin by H2O2. We describe an artifact in this method that may explain the seeming...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087899
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:We have previously reported that N-acetylcysteine (NAC), ambroxol and azithromycin (AZM) (partially) correct the chloride efflux dysfunction in cystic fibrosis bronchial epithelial (CFBE) cells with the ΔF508 homozygous mutation in vitro. METHODS:In the present paper, we further investigated p...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2014.934411
更新日期:2015-06-01 00:00:00
abstract::We determined optimum conditions for delivering DNA to transformed human bronchial epithelial cells expressing wild-type (BEAS) or abnormal (2CF) cystic fibrosis transmembrane conductance regulator (CFTR) using cationic liposomes (Lipofectin, [N-(N,N-dimethylaminoethane)carbamyl] cholesterol[DC-Chol]/dioleoylphosphati...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270259
更新日期:1999-04-01 00:00:00
abstract::The objective of this study was to assess the radioprotective effects of amifostine in the rat model of radiation-induced lung injury using fractionated doses of radiation, to determine whether amifostine given before irradiation protects tumor from radiation cytotoxicity, and to determine whether changes in plasma le...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140290096791
更新日期:2002-10-01 00:00:00
abstract::Acute lung injury (ALI) is often associated with sepsis and is the most common cause of acute respiratory failure. The authors evaluated the role of the heme oxygenase (HO)/carbon monoxide (CO) system on lung injury in a cecal ligation and puncture (CLP)-induced mouse model of ALI. The authors established CLP-induced ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2012.660559
更新日期:2012-05-01 00:00:00
abstract::Inflammation plays a key role in lung injury and in the pathogenesis of asthma. Two murine models of allergic airway inflammation-sensitization and challenge to ovalbumin (OVA) and intratracheal exposure to interleukin-13 (IL13)-were used to evaluate the expression of poly(ADP-ribose) polymerase-1 (PARP-1) in allergic...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902141003663360
更新日期:2010-09-01 00:00:00
abstract::The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of primary importance. Various pathological processes affect the extracellular matrix (ECM...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.1080/019021490944232
更新日期:2005-07-01 00:00:00
abstract::Lung cancer is the leading cause of death of both men and women across the world. Overexpression and activating mutations of the epidermal growth factor receptor-1 (EGFR1) are frequently observed and associated with poor prognosis. To inhibit the function of EGFR1, multiple antibodies and small-molecule tyrosine kinas...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.831960
更新日期:2013-11-01 00:00:00
abstract::Volcanic ash was collected from the Moses Lake region of Washington State after the 18 May 1980 eruption of Mt. St. Helens. The ash was tested in a short-term bioassay system using hamsters exposed by intratracheal instillation. One day after exposure the lungs were lavaged and the fluid collected was characterized us...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148109052324
更新日期:1981-11-01 00:00:00
abstract::Understanding the effects from long-term exposure to individual ambient air pollutants and mixtures of pollutants is necessary for adequate assessment of health risk. This study examined quantitative and temporal alterations in tracheobronchial mucociliary clearance function and bronchial epithelial secretory cells in...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149209064343
更新日期:1992-07-01 00:00:00
abstract::This study describes the morphology of the adaptive response of the pulmonary parenchyma after the resection of lung tissue. Rats aged 23 days were subjected to the bilobectomy of the right upper and middle lobes representing 25% of the total lung volume. On the postoperative Days 1, 4, 6, 9, 12, 18, and 30, the lungs...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148209039246
更新日期:1982-12-01 00:00:00
abstract::The morphology and phagocytic capacities of pulmonary macrophages recovered by lavage from normal rats were studied in vitro after incubation under various culture conditions. Most adherent macrophages (73 +/- 6%) were flat and exhibited ruffled surfaces. Phagocytosis of carbonyl iron beads by pulmonary macrophages wa...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148409087896
更新日期:1984-01-01 00:00:00
abstract::It is evident that much remains to be learned about the nasal passages and their responses to toxic materials. For the nose of both laboratory animals and humans, information is needed in the areas of anatomy, physiology, biochemistry, neurobiology, physiopathology, and oncology. This article briefly discussed toxic a...
journal_title:Experimental lung research
pub_type: 杂志文章,评审
doi:10.3109/01902149109064322
更新日期:1991-09-01 00:00:00
abstract::Excess production of neutrophil elastase contributes to the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the role of neutrophil elastase in the repair process following ALI/ARDS is not well understood. The objective of this study was to evaluate the effect of neutrop...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2011.633306
更新日期:2012-02-01 00:00:00
abstract::We quantified in vivo pulmonary vascular remodeling in a large animal model of pulmonary hypertension (PH). In group PH (n = 6), 3 mg/kg dehydromonocrotaline (DHMC) was administered to 12-week-old beagles via a right atrial injection. Eight weeks after DHMC in group PH, pulmonary artery pressure increased significantl...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401459734
更新日期:2001-01-01 00:00:00
abstract::Protein tyrosine phosphatase is an important class of enzymes that plays an essential role in the cellular proliferation, differentiation, and oncogenesis. In this paper we report characterization of a low-molecular-weight protein tyrosine phosphatase purified from bovine lung. The enzyme activity was essentially inde...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902149809041534
更新日期:1998-05-01 00:00:00
abstract::B6C3F1 female mice were exposed to cigarette smoke (CS) (250 mg/m3 total particulate material) or filtered air (FA), 6 hours/day, 5 days/week, for 6, 7, or 10 weeks, or to CS for 6 weeks, then FA for 1 or 4 additional weeks. Exposure to CS increased macrophages, neutrophils, lymphocytes, and matrix metalloproteinase (...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140490252858
更新日期:2004-01-01 00:00:00
abstract::Aims:Bronchopulmonary dysplasia (BPD) is characterized by alveolarization arrest. During alveolarization, alveolar myofibroblasts are thought to migrate into the septal tips and elongate secondary septa. Lipopolysaccharide (LPS) exposure has been reported to disrupt directional migration and final location of alveolar...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902148.2019.1711464
更新日期:2020-01-01 00:00:00
abstract::Thrombin activates protease-activated receptor (PAR)-1 and induces a myofibroblast phenotype in normal lung fibroblasts. The origins of myofibroblasts are resident fibroblasts, fibrocytes, and epithelial-mesenchymal transition (EMT). We investigated the effects of thrombin, an important mediator of interstitial lung f...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.3109/01902148.2013.820809
更新日期:2013-10-01 00:00:00
abstract::The streptozotocin-induced diabetic (STZ-DB) rat model is associated with fetal hyperglycemia, but with low to normal plasma insulin concentration. Because surfactant protein (SP) mRNA content in fetal rat lung is decreased in STZ-DB pregnancy, we investigated the effect of increasing concentrations of glucose on SP g...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021499270439
更新日期:1999-01-01 00:00:00
abstract::Long-term, low-dose macrolide therapy has been proven to improve survival in patients with diffuse panbronchiolitis and cystic fibrosis, although the mechanisms by which it does so remain unknown. To elucidate the molecular mechanisms of the anti-inflammatory effects of macrolides, the authors examined the effects of ...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/01902140802093238
更新日期:2008-08-01 00:00:00
abstract::Leukotrienes (LTs) are potent vasoconstrictors in the pulmonary circulation. We investigated LTB4 and LTE4 metabolism by intrapulmonary arteries and veins of 2 to 9 days old lambs (n = 6). Paired vessels were incubated under baseline, and stimulated conditions. LTB4 and LTE4 were extracted from media, quantfied by enz...
journal_title:Experimental lung research
pub_type: 杂志文章
doi:10.1080/019021401750193601
更新日期:2001-06-01 00:00:00