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The "Trojan horse" model-delivery of anti-HBV small interfering RNAs by a recombinant HBV vector.

Abstract：

:Hepatitis B virus (HBV) is a small virus that infects the liver. The major obstacle in applying the RNA interference method as an anti-HBV weapon is the challenge to deliver the small interfering RNA molecules to the liver efficiently and specifically. Here we show that HBV-specific short hairpin RNAs (shRNAs) are efficiently expressed from a recombinant HBV into which an shRNA-expressing cassette was inserted, resulting in a significant knock-down of HBV gene expression. Notably, this recombinant HBV still expresses the HBV Core protein, which is targeted by the shRNAs produced by the same vector. Our results set the stage for further use of this recombinant HBV virus with the potential to function as a "Trojan horse"; one that specifically targets the liver and uses the resident virus as an helper for its own propagation, and at the same time eliminate itself and the resident HBV by knocking-down their gene expression.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Shlomai A,Lubelsky Y,Har-Noy O,Shaul Y

doi

10.1016/j.bbrc.2009.10.016

subject

Has Abstract

pub_date

2009-12-18 00:00:00

pages

619-23

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01980-9

journal_volume

390

pub_type

杂志文章

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