Involvement of adenosine kinase in the phosphorylation of formycin B in CHO cells.

abstract：:Gene delivery has become an important tool for biological research and gene therapy trials. Lentiviral vector (LV) mediated gene transfer is a preferred approach for stable, sustained transgenic expression. We report here step-wise development of an Indian human immunodeficiency virus type 2 (HIV-2) isolate derived th...

journal_title：Biochemical and biophysical research communications

authors： Santhosh CV,Tamhane MC,Kamat RH,Patel VV,Mukhopadhyaya R

更新日期：2008-07-04 00:00:00

abstract：:A protein extract prepared from the egg shell fiber-secreting region of the oviduct of Alligator mississippiensis was assayed for the presence of receptors for estrogen and progesterone. In the presence of [3H]-estradiol-17 beta, the extract contained an estrogen-binding activity which reached equilibrium at 25 degree...

journal_title：Biochemical and biophysical research communications

authors： Vonier PM,Guillette LJ Jr,McLachlan JA,Arnold SF

更新日期：1997-03-17 00:00:00

abstract：:Brassica campestris Male Fertility 20 (BcMF20) is a typical zinc-finger transcription factor that was previously isolated from flower buds of Chinese cabbage (Brassica campestris ssp. chinensis). By applying expression pattern analysis, it can be known that BcMF20 was specifically and strongly expressed in tapetum and...

journal_title：Biochemical and biophysical research communications

authors： Han YY,Zhou HY,Xu LA,Liu XY,Fan SX,Cao JS

更新日期：2018-09-05 00:00:00

abstract：:Low voltage-activated, rapidly inactivating T-type Ca(2+) channels are found in a variety of cells where they regulate electrical activity and Ca(2+) entry. In whole-cell patch clamp recordings from bovine adrenal zona fasciculata cells, cis-polyunsaturated omega-3 fatty acids including docosahexaenoic acid (DHA), eic...

journal_title：Biochemical and biophysical research communications

authors： Danthi SJ,Enyeart JA,Enyeart JJ

更新日期：2005-02-11 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) [1,25(OH)(2) D(3)] exerts its biological effects by binding to the vitamin D receptor (VDR), which binds in turn to the vitamin D response elements located in the target gene's promoter. We have previously demonstrated that VDR binds in vitro with high affinity to the 5'-flanking sequence of...

journal_title：Biochemical and biophysical research communications

authors： Seoane S,Alonso M,Segura C,Pérez-Fernández R

更新日期：2002-03-22 00:00:00

abstract：:Rat mast cell protease of type 1 (RMCP1) is a specific marker of connective tissue mast cells selectively occurring in some tissues, e.g., the tongue. Its amino acid sequence is known (Le Trong et al., Biochem. 1987, 26, 6988-6994) but not the corresponding nucleotide sequence. Amplification of mRNAs from rat tongue w...

journal_title：Biochemical and biophysical research communications

authors： Rouleau A,Garbarg M,Schwartz JC,Ruat M

更新日期：1994-03-15 00:00:00

abstract：:To study polygenetically inherited human diseases like hypertension, inbred rat strains are usually the preferred models. Because many inbred generations under optimized environmental conditions may have led to the survival of "silent" disease genes, we used a cross between one wild rat and genetically hypertensive SH...

journal_title：Biochemical and biophysical research communications

authors： Klöting I,Kovács P,van den Brandt J

更新日期：2001-06-29 00:00:00

abstract：:We propose that a substance, identified using mass spectrometry, present in the hemolymph of the honeybee (Apis mellifera) as a result of stimulating the insect immune system corresponds with Apidaecin I, an antibacterial peptide recently described. The plasma desorption mass spectra indicate that several other higher...

journal_title：Biochemical and biophysical research communications

authors： Craig AG,Trenczek T,Fries I,Bennich H

更新日期：1989-12-15 00:00:00

abstract：:Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to th...

journal_title：Biochemical and biophysical research communications

authors： Di Filippo ES,Mancinelli R,Pietrangelo T,La Rovere RM,Quattrocelli M,Sampaolesi M,Fulle S

更新日期：2016-04-29 00:00:00

abstract：:Although the regeneration of the adult liver depends on hepatic progenitor cells (HPCs), many uncertainties regarding hepatic regeneration in the injured liver remain. Trefoil factor family 1 (TFF1), a secretory protein predominantly expressed in the gastrointestinal tract, is responsible for mucosal restitution. Here...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Yamaguchi J,Kokuryo T,Ebata T,Yokoyama Y,Nagino M

更新日期：2018-11-17 00:00:00

abstract：:Bacteria belonging to the Burkholderia cepacia complex (BCC) are important opportunistic pathogens in patients with cystic fibrosis (CF). Since approximately 80% of the CF isolates examined produce exopolysaccharide (EPS), it was hypothesized that this EPS may play a role in the colonization and persistence of these b...

journal_title：Biochemical and biophysical research communications

authors： Moreira LM,Videira PA,Sousa SA,Leitão JH,Cunha MV,Sá-Correia I

更新日期：2003-12-12 00:00:00

abstract：:We describe a method for the removal of endogenous troponin (Tn) complex from bundles of detergent-treated cardiac fibers. After 70 min treatment with cTnT-cTnI most of the endogenous Tn complex was removed from fiber bundles. Complete reconstitution of the Tn complex was achieved by reconstituting with cardiac tropon...

journal_title：Biochemical and biophysical research communications

authors： Chandra M,Kim JJ,Solaro RJ

更新日期：1999-09-16 00:00:00

abstract：:In order to investigate tooth development, several cell lines of the dental epithelium and ectomesenchyme have been established. However, no attempt has been reported to regenerate teeth with cell lines. Here, we have established several clonal cell lines of the dental epithelium from a p53-deficient fetal mouse. They...

journal_title：Biochemical and biophysical research communications

authors： Komine A,Suenaga M,Nakao K,Tsuji T,Tomooka Y

更新日期：2007-04-13 00:00:00

abstract：:Here we enhanced the stability and biophysical properties of mRFP1 through a combination of canonical and non-canonical amino acid mutagenesis. The global replacement of proline residue with (2S, 4R)-4-fluoroproline [(4R)-FP] into mRFP1 led to soluble protein but lost its fluorescence, whereas (2S, 4S)-4-fluoroproline...

journal_title：Biochemical and biophysical research communications

authors： Deepankumar K,Nadarajan SP,Ayyadurai N,Yun H

更新日期：2013-11-01 00:00:00

abstract：:Metabolic assay buffers often omit bicarbonate, which is susceptible to alkalinisation in an open environment. Here, we assessed the effect of including bicarbonate in respirometry experiments. By supplementing HEPES-buffered media with low concentrations of bicarbonate, we found increased respiration in adipocytes an...

journal_title：Biochemical and biophysical research communications

authors： Krycer JR,Fisher-Wellman KH,Fazakerley DJ,Muoio DM,James DE

更新日期：2017-08-05 00:00:00

abstract：:The UDP-GalNAc:NeuAc alpha 2,3Gal beta-R (GalNAc to Gal) beta-1,4-N-acetylgalactosaminyltransferase (beta 1,4GalNAc-transferase) is the enzyme responsible for the addition of the immunodominant sugar of the Sda isto-blood group determinant. In humans the enzyme is mainly expressed in the large intestine. We screened n...

journal_title：Biochemical and biophysical research communications

authors： Malagolini N,Dall'Olio F,Serafini-Cessi F

更新日期：1991-10-31 00:00:00

abstract：:The glutathione reductase gene, gor, was cloned from the plant pathogen Xanthomonas campestris pv. phaseoli. Its gene expression and enzyme characteristics were found to be different from those of previously studied homologues. Northern blot hybridization, promoter-lacZ fusion, and enzyme assay experiments revealed th...

journal_title：Biochemical and biophysical research communications

authors： Loprasert S,Whangsuk W,Sallabhan R,Mongkolsuk S

更新日期：2005-06-17 00:00:00

abstract：:In Saccharomyces cerevisiae, the bud site selection of diploid cells is regulated by at least four persistent landmarks, Bud8p, Bud9p, Rax1p, and Rax2p. Bud8p and Bud9p are essential for the establishment of bipolar budding and localize mainly to the distal and the proximal poles, respectively. Their subcellular local...

journal_title：Biochemical and biophysical research communications

authors： Kato Y,Kawasaki H,Arakawa N,Hirano H

更新日期：2010-09-03 00:00:00

abstract：:This study examined the actions of L-arginine, a putative precursor of endothelium-derived nitric oxide, and arginine analogs on endothelium-dependent relaxation of isolated rings of bovine pulmonary artery. L-Arginine did not consistently relax arterial rings unless rings were first rendered refractory to endothelium...

journal_title：Biochemical and biophysical research communications

authors： Gold ME,Wood KS,Buga GM,Byrns RE,Ignarro LJ

更新日期：1989-06-15 00:00:00

abstract：:Osteogenic protein-1 (OP-1) is a member of the bone morphogenetic protein subfamily of the transforming growth factor-beta (TGF-beta) superfamily. Since members of the TGF-beta superfamily have a role in tissue development the distribution of OP-1 in developing human embryos (gestational age 5-14 weeks) was examined b...

journal_title：Biochemical and biophysical research communications

authors： Vukicevic S,Latin V,Chen P,Batorsky R,Reddi AH,Sampath TK

更新日期：1994-01-28 00:00:00

abstract：:Ribonuclease P (RNase P) is an endonuclease responsible for generating the 5(') end of matured tRNA molecules. A homology search of the hyperthermophilic archaeon Pyrococcus horikoshii OT3 genome database revealed that the four genes, PH1481, PH1601, PH1771, and PH1877, have a significant homology to those encoding RN...

journal_title：Biochemical and biophysical research communications

authors： Kouzuma Y,Mizoguchi M,Takagi H,Fukuhara H,Tsukamoto M,Numata T,Kimura M

更新日期：2003-07-04 00:00:00

abstract：:Parkinson's disease (PD) is neurodegenerative diseases caused by the loss of dopaminergic neurons in the substantia nigra pars compacta. Stem cell therapy is one of the promising strategies in helping to cure PD. In the present study, human mesenchymal stem cells (MSCs) from eye conjunctiva stromal cells were differen...

journal_title：Biochemical and biophysical research communications

authors： Nadri S,Soleimani M,Mobarra Z,Amini S

更新日期：2008-12-12 00:00:00

abstract：:NADH-rubredoxin oxidoreductase (NROR), a flavoprotein from the obligately anaerobe Clostridium acetobutylicum is encoded by an ORF (nror) of 1140 nucleotides. Whereas primary structure analysis reveals that NROR has amino acid sequence patterns homologous with those involved in FAD and NAD-binding, the enzyme is dista...

journal_title：Biochemical and biophysical research communications

authors： Guedon E,Petitdemange H

更新日期：2001-07-13 00:00:00

abstract：:Pinin (Pnn) is a nuclear speckle-associated SR-like protein. The N-terminal region of the Pnn protein sequence is highly conserved from mammals to insects, but the C-terminal RS domain-containing region is absent in lower species. The N-terminal coiled-coil domain (CCD) is, therefore, of interest not only from a funct...

journal_title：Biochemical and biophysical research communications

authors： Wu HP,Hsu SY,Wu WA,Hu JW,Ouyang P

更新日期：2014-01-03 00:00:00

abstract：:The murine C-5 cytosine DNA methyltransferase (MTase, E.C.2.1.1.37) containing a hexahistidine affinity leader peptide has been expressed at levels which are at least 50-fold higher than previously reported. The recombinant enzyme has activity levels similar to the wild-type enzyme. The recombinant polypeptide binds t...

journal_title：Biochemical and biophysical research communications

authors： Glickman JF,Reich NO

更新日期：1994-11-15 00:00:00

abstract：:Polymerization of actin induced by activation of platelets was investigated using deoxyribonuclease I inhibition assay. When platelets were activated with ADP or 5-hydroxytryptamine, actin was polymerized quickly followed by rapid depolymerization to the initial level. Reactivation with the same agonist, however, did ...

journal_title：Biochemical and biophysical research communications

authors： Imada T,Kikuchi T,Shimada H,Inada Y,Saito Y

更新日期：1984-05-16 00:00:00

abstract：:Trypsin-like serine proteases are involved in diverse biological processes such as complement activation, tissue remodeling, cellular migration, tumor invasion, and metastasis. Here we report a novel human C1r-like serine protease analog, CLSPa, derived from dendritic cells (DC). The 487-residue CLSPa protein contains...

journal_title：Biochemical and biophysical research communications

authors： Lin N,Liu S,Li N,Wu P,An H,Yu Y,Wan T,Cao X

更新日期：2004-08-20 00:00:00

abstract：:The senescence-accelerated mouse prone 8 (SAMP8) strain exhibits age-related learning and memory deficits (LMD) at 2 months of age. Combined linkage analysis of 264 F2 intercross SAMP8 × JF1 mice and RNA-seq analysis identified Hcn1 gene out of 29 genes in the LMD region on chromosome 13. Hcn1 in SAMP8 strain showed 1...

journal_title：Biochemical and biophysical research communications

authors： Akbor MM,Tomobe K,Yamada T,Kim J,Mano H,Kurosawa N,Sasaki K,Nomura Y,Isobe M

更新日期：2013-11-08 00:00:00

abstract：:In this study, we demonstrate that in addition to T lymphocytes, human naïve eosinophils and the differentiated eosinophil-like cell line, AML14.3D10 express CCR8 and respond to CCL1 through CCR8 engagement. The responsiveness of cells was dependent on maturation stage, since CCL1 induced pronounced chemotaxis only in...

journal_title：Biochemical and biophysical research communications

authors： Karlsson AK,Walles K,Bladh H,Connolly S,Skrinjar M,Rosendahl A

更新日期：2011-04-22 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) play crucial roles in tumor development of osteosarcoma (OS). LncRNA PCAT6 was involved in the progression of multiple human cancers. However, the biological function of PCAT6 in OS remains largely unknown. We found that PCAT6 was elevated in OS tissues relative to that in their adjacent ...

journal_title：Biochemical and biophysical research communications

authors： Zhu C,Huang L,Xu F,Li P,Li P,Hu F

更新日期：2020-01-08 00:00:00