Abstract:
:Here we tested the segregation and paternal compatibility of markers INRA124 and INRA126 on female DNA in 10 different cattle families, in order to clarify the usefulness of these microsatellites for the study of male-mediated population processes in cattle. Their performance was compared with that of four microsatellites located in the PAR-BTAY (UMN0108, UMN0803, UMN0929 and UMN0905) and another one male-specific microsatellite (INRA189). INRA124 and INRA126 amplified the same sized fragment in both sexes. Same size alleles were sequenced and the high homology found allowed us to rule out non-specific female amplification. INRA124 showed full parental compatibility, whilst the locus INRA126 showed 55% parental incompatibility. Based on these observations, it is recommended that markers INRA124 and INRA126 should not be used in studies to characterize male-mediated genetic events in cattle.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Pérez-Pardal L,Royo LJ,Alvarez I,de León FA,Fernández I,Casais R,Goyache Fdoi
10.1111/j.1365-2052.2009.01870.xsubject
Has Abstractpub_date
2009-08-01 00:00:00pages
560-4issue
4eissn
0268-9146issn
1365-2052pii
AGE1870journal_volume
40pub_type
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