Female segregation patterns of the putative Y-chromosome-specific microsatellite markers INRA124 and INRA126 do not support their use for cattle population studies.

Abstract:

:Here we tested the segregation and paternal compatibility of markers INRA124 and INRA126 on female DNA in 10 different cattle families, in order to clarify the usefulness of these microsatellites for the study of male-mediated population processes in cattle. Their performance was compared with that of four microsatellites located in the PAR-BTAY (UMN0108, UMN0803, UMN0929 and UMN0905) and another one male-specific microsatellite (INRA189). INRA124 and INRA126 amplified the same sized fragment in both sexes. Same size alleles were sequenced and the high homology found allowed us to rule out non-specific female amplification. INRA124 showed full parental compatibility, whilst the locus INRA126 showed 55% parental incompatibility. Based on these observations, it is recommended that markers INRA124 and INRA126 should not be used in studies to characterize male-mediated genetic events in cattle.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Pérez-Pardal L,Royo LJ,Alvarez I,de León FA,Fernández I,Casais R,Goyache F

doi

10.1111/j.1365-2052.2009.01870.x

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

560-4

issue

4

eissn

0268-9146

issn

1365-2052

pii

AGE1870

journal_volume

40

pub_type

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