Abstract:
:This study deals with the morphofunctional influence of 72 h exposure to a 6 mT static magnetic field (SMF) during differentiation induced by 50 ng/ml 12-O-tetradecanoyl-13-phorbol acetate (TPA) in human leukaemia U937 cells. The cell morphology of U937 cells was investigated by optic and electron microscopy. Specific antibodies and/or molecules were used to label CD11c, CD14, phosphatidylserine, F-actin and to investigate the distribution and activity of lysosomes, mitochondria and SER. [Ca(2+)](i) was evaluated with a spectrophotometer. The degree of differentiation in SMF-exposed cells was lower than that of non-exposed cells, the difference being exposure time-dependent. SMF-exposed cells showed cell shape and F-actin modification, inhibition of cell attachment, appearance of membrane roughness and large blebs and impaired expression of specific macrophagic markers on the cell surface. The intracellular localization of SER and lysosomes was only partially affected by exposure. A significant localization of mitochondria with an intact membrane potential at the cell periphery in non-exposed, TPA-stimulated cells was observed; conversely, in the presence of SMF, mitochondria were mainly localised near the nucleus. In no case did SMF exposure affect cell viability. The sharp intracellular increase of [Ca(2+)](i) could be one of the causes of the above-described changes.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Dini L,Dwikat M,Panzarini E,Vergallo C,Tenuzzo Bdoi
10.1002/bem.20474subject
Has Abstractpub_date
2009-07-01 00:00:00pages
352-64issue
5eissn
0197-8462issn
1521-186Xjournal_volume
30pub_type
杂志文章abstract::Occupational exposure to the high static magnetic fields (SMFs) during magnetic resonance imaging (MRI) examinations raises concerns of adverse health effects. In this study, personal exposure monitoring of the magnetic fields during routine examinations in two 3 T MRI systems was carried out. A three-axis Hall magnet...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,多中心研究
doi:10.1002/bem.21817
更新日期:2014-01-01 00:00:00
abstract::The electromagnetic exposure of cell phone users depends on several parameters. One of the most dominant of these is the distance between the cell phone and the head tissue. The pinna can be regarded as a spacer between the top of the phone and the head tissue. The size of this spacer has not yet been systematically s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20575
更新日期:2010-07-01 00:00:00
abstract::The potential effects of radiofrequency (RF) exposure on the genetic material of cells are very important to determine since genome instability of somatic cells may be linked to cancer development. In response to genetic damage, the p53 protein is activated and can induce cell cycle arrest allowing more time for DNA r...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21744
更新日期:2013-01-01 00:00:00
abstract::Research conducted over the past 5 years has addressed the hypothesis that the reported association between residential magnetic fields and childhood leukemia may be explained by exposure to contact current. The use of multi-grounded neutrals in electrical distribution and residential electrical wiring systems in the ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/bem.20126
更新日期:2005-01-01 00:00:00
abstract::In studies investigating adverse reproductive outcomes associated with video display terminal (VDT) usage, amounts of time spent in front of a VDT or magnetic field (MF) levels in front of the VDT are used as surrogate measures of subject's MF exposure. However, the relevance of such surrogates to actual exposures has...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(SICI)1521-186X(1996)17:5<406::AID-BEM8>3.
更新日期:1996-01-01 00:00:00
abstract::The accurate and valid measurement of personal exposure to magnetic fields poses a major challenge for epidemiologic studies. When considering the various methods to assess exposure, it is unclear which measures are most relevant for studies of human disease, if any. Given these uncertainties, the Electromagnetic Fiel...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Digital cellular telephones using the GSM (Global System for Mobile Communication) transmit information in bursts of microwaves. This pulsed transmitting mode causes the battery current and currents in the electronics of the apparatus to be pulsed. These pulsed currents produce corresponding pulsed magnetic fields nea...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorb...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10120
更新日期:2003-10-01 00:00:00
abstract::Measurements have been made of vector current densities induced by vertical, uniform, 60-Hz electric fields in the torsos of homogeneous models of swine and rats. The observed data were a strong function of the five grounding configurations invested: all four feet grounded, only front feet grounded, only rear feet gro...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250090102
更新日期:1988-01-01 00:00:00
abstract::This article presents measurement methods used to determine the human exposure to electromagnetic fields radiated from operating base stations. In Korea, when evaluating the human exposure to electromagnetic fields from operating base stations, the measurement procedure is different between the following cases: in sit...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20416
更新日期:2008-10-01 00:00:00
abstract::The mechanism by which animals detect weak electric and magnetic fields has not yet been elucidated. We propose that transduction of an electric field (E) occurs at the apical membrane of a specialized cell as a consequence of an interaction between the field and glycoproteins bound to the gates of ion channels. Accor...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20310
更新日期:2007-07-01 00:00:00
abstract::Our previous studies showed that extremely low frequency magnetic fields (ELF-MFs) inhibited tumor growth and change proportion of splenic regulatory T cells (Treg cells). Here, we focus on the effect of ELF-MFs on lung metastatic melanoma mouse model and the regulatory mechanism of ELF-MFs on the differentiation of T...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21954
更新日期:2016-02-01 00:00:00
abstract::The aim of the present study was to determine millimeter wave (MMW) absorption by blood vessels traversing the subcutaneous fat layer of murine skin. Most calculations were performed using the finite-difference time-domain (FDTD) technique. We used two types of models: (1) a rectangular block of multilayer tissue with...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20658
更新日期:2011-09-01 00:00:00
abstract::Cell-based or magnetic field therapies as alternative approaches to pain management have been tested in several experimental pain models. The aim of this study therefore was to investigate the actions of the cell-based therapy (adipose tissue derived mesenchymal stem cells; ADMSC) or pulsed magnetic field (PMF) therap...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22038
更新日期:2017-05-01 00:00:00
abstract::In a previous study, we showed that operators of radiofrequency (RF) plastic sealers, RF operators (n = 35) had a lower heart rate during nighttime compared to a control group (n = 37). We have analyzed the heart rate variability (HRV) on the same group of people to better understand the possible underlying rhythm dis...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20276
更新日期:2007-01-01 00:00:00
abstract::The use of prosthetic implants is increasing both in the United States and around the world and there is a concomitant rise in cases of biofilm-based, persistent infections that are quite serious and virtually impervious to antibiotic treatment. The development of alternate therapies that do not involve long term use ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20529
更新日期:2010-01-01 00:00:00
abstract::This study examined the time dependence effects of exposure to radiofrequency radiation (RFR) emitted by standard GSM cellular phones on the cognitive functions of humans. A total of 48 healthy right-handed male subjects performed a spatial working memory task (that required either a left-hand or a right-hand response...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20458
更新日期:2009-04-01 00:00:00
abstract::In view of possible therapeutic applications of magnetic fields, the effect of an enhancement of neuronal outgrowth at higher figures of flux density and induced field strength was investigated. On the average sinusoidal magnetic field treatment at 100 microTrms/50 Hz did not change nerve growth factor (NGF) induced n...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20073
更新日期:2005-01-01 00:00:00
abstract::We have studied the effect of extremely low frequency electromagnetic fields (ELF-EMF) in the presence of a second stressor (mild heat shock) on the expression of a lacZ reporter gene under the control of hsp16 or hsp70 promoters in two transgenic strains of C. elegans. The expression of the reporter gene was studied ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(200002)21:2<100::aid-bem4>
更新日期:2000-02-01 00:00:00
abstract::We present a simple readout device that fills the void produced by the gradual disappearance of the Vitck model 101 Electrothermia Monitor. The new device uses commercially available probes that are similar to Bowman's (IEEE Trans Microwave Theory Tech MTT-24:43-45, 1976) original design. As described, the device cove...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250100210
更新日期:1989-01-01 00:00:00
abstract::Previously, we have reported that exposure to 50 Hz coherent sinusoidal magnetic fields (MF) for 24 h inhibits gap junction intercellular communication (GJIC) in mammalian cells at an intensity of 0.4 mT and enhances the inhibition effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) at 0.2 mT. In the present study, w...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20207
更新日期:2006-05-01 00:00:00
abstract::The objective of this study was to investigate if persons with implantable medical devices are intrinsically protected by the current electromagnetic safety guidelines. For inter-laboratory comparisons, the U.S. Food and Drug Administration has defined a generic implant as consisting of an insulated wire with noninsul...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章
doi:10.1002/bem.21695
更新日期:2012-07-01 00:00:00
abstract::We study the effect of microwaves at 2,450 MHz on protein unfolding using surface plasmon resonance sensing. Our experimental method makes use of the fact that unfolding proteins tend to bind to chaperones on their unfolding pathway and this attachment is readily monitored by surface plasmon resonance. We use the prot...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20382
更新日期:2008-05-01 00:00:00
abstract::To be able to correlate real time membrane potential or ion flux changes with further downstream gene transcription responses due to extremely low frequency (ELF) electromagnetic field (EMF) exposure, we devised an experimental system consisting of a pair of symmetric circular coils. This system can be used on an inve...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10151
更新日期:2004-01-01 00:00:00
abstract::The current through the residential grounding circuit is an important source for magnetic fields; field variations near the grounding circuit accurately track fluctuations in this ground current. In this paper, a model is presented which permits calculation of the range of these fluctuations. A discrete network model ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250150109
更新日期:1994-01-01 00:00:00
abstract::The aim of this study was to determine a current density threshold for exciting the motor cortex area of the brain. The current density threshold for excitation of nerve fibres (20 microm in diameter) found in the literature is approximately 1 A/m(2) at frequencies lower than 1 kHz. In consideration of a safety factor...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10036
更新日期:2002-09-01 00:00:00
abstract::Blackman et al. [1994] describe an experiment that purports to show that weak 45 Hz magnetic fields inhibit the growth of neurites from PC-12 cells treated with a growth stimulation factor. I present a statistical analysis of the data in that paper that shows that the data were corrupted in some way; hence, the result...
journal_title:Bioelectromagnetics
pub_type: 评论,杂志文章
doi:10.1002/(SICI)1521-186X(1996)17:6<510::AID-BEM12>3
更新日期:1996-01-01 00:00:00
abstract::We sought to determine whether a 6-week exposure to a 50-Hz rotating magnetic field influences melatonin synthesis by 11-18 week-old Wistar-King male rats. Rats were exposed continuously to a rotating magnetic field at 1, 5, 50, or 250 microT (spatial vector rms) for 6 weeks, except for twice-weekly breaks of about 2 ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250140203
更新日期:1993-01-01 00:00:00
abstract::To investigate the effects of 1.8 GHz radiofrequency (RF) field on bone microstructure and metabolism of femur in mice, C57BL/6 mice (male, age 4 weeks) were whole-body exposed or sham exposed to 1.8 GHz RF field. Specific absorption rates of whole body and bone were approximately 2.70 and 1.14 W/kg (6 h/day for 28 da...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22125
更新日期:2018-07-01 00:00:00
abstract::The stimulatory effects on germination of seeds and growth of plants of static magnetic field (MF) pre-treatments depending on MF intensity, exposure time periods, signal form, flux density, and source frequencies on plants are reported. Seed germination frequency is low due to dormancy in Lathyrus chrysanthus Boiss. ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22139
更新日期:2018-10-01 00:00:00