Abstract:
:We study the effect of microwaves at 2,450 MHz on protein unfolding using surface plasmon resonance sensing. Our experimental method makes use of the fact that unfolding proteins tend to bind to chaperones on their unfolding pathway and this attachment is readily monitored by surface plasmon resonance. We use the protein citrate synthase (CS) for this study as it shows strong binding to the chaperone alpha crystallin when stressed by exposure to excess temperature. The results of microwave heating are compared with the effect of ambient heating and a combination of ambient and microwave heating to the same final temperature. We study the temperature distributions during the heating process. We show that microwaves cause a significantly higher degree of unfolding than conventional thermal stress for protein solutions heated to the same maximum temperature.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
George DF,Bilek MM,McKenzie DRdoi
10.1002/bem.20382subject
Has Abstractpub_date
2008-05-01 00:00:00pages
324-30issue
4eissn
0197-8462issn
1521-186Xjournal_volume
29pub_type
杂志文章abstract::This study analyzes if an external magnetic stimulus (2 kHz and approximately 0.1 μT applied near frontal cortex) influences working memory, perception, binary decision, motor execution, and sustained attention in humans. A magnetic stimulus and a sham stimulus were applied to both sides of the head (frontal cortex cl...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21944
更新日期:2016-01-01 00:00:00
abstract::The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorb...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10120
更新日期:2003-10-01 00:00:00
abstract::This study deals with the morphofunctional influence of 72 h exposure to a 6 mT static magnetic field (SMF) during differentiation induced by 50 ng/ml 12-O-tetradecanoyl-13-phorbol acetate (TPA) in human leukaemia U937 cells. The cell morphology of U937 cells was investigated by optic and electron microscopy. Specific...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20474
更新日期:2009-07-01 00:00:00
abstract::We developed a new computer wire coding method and then applied it to investigate the suggestion that control selection bias might explain the observed association between wire codes and childhood cancer made in the study conducted by Savitz et al. in the Denver area. The computer wire coding method used a geographic ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::In this study, we measured and analyzed the spectral characteristics of a low-frequency magnetic field (MF) inside several gasoline-powered cars while driving on busy city roads. The spectra obtained upon measurements in the interior of the cars are compared with those measured in office locations at different times o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22269
更新日期:2020-07-01 00:00:00
abstract::The dielectric behavior of the aqueous solutions of three widely differing macromolecules has been investigated: myoglobin, polyvinylpyrrolidone (PVP), and human serum low-density lipoprotein (LDL). It was not possible to interpret unambiguously the dielectric properties of the PVP solution in terms of water structure...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070206
更新日期:1986-01-01 00:00:00
abstract::The mechanism by which animals detect weak electric and magnetic fields has not yet been elucidated. We propose that transduction of an electric field (E) occurs at the apical membrane of a specialized cell as a consequence of an interaction between the field and glycoproteins bound to the gates of ion channels. Accor...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20310
更新日期:2007-07-01 00:00:00
abstract::The temperature distribution inside a lossy sphere resulting from the absorption of microwave energy was approximated by successive numerical iterations. of the thermal energy equation. Heat transfer within the sphere by conduction was considered. In the model energy was not dissipated by convection but was contained ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250010309
更新日期:1980-01-01 00:00:00
abstract::C57/B1 mice were exposed during pregnancy (gestation days 0-19) to a 20 kHz magnetic field (MF). The asymmetric sawtooth-waveform magnetic field in the exposed racks had a flux density of 15 microT (peak to peak). After 19 days, the exposure was terminated, and the mice were housed individually under normal laboratory...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160409
更新日期:1995-01-01 00:00:00
abstract::Ejaculated, density purified, human spermatozoa were exposed to pulsed 900 MHz GSM mobile phone radiation at two specific absorption rate levels (SAR 2.0 and 5.7 W/kg) and compared with controls over time. Change in sperm mitochondrial membrane potential was analysed using flow cytometry. Sperm motility was determined...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20390
更新日期:2008-05-01 00:00:00
abstract::Extremely low-frequency (ELF) magnetic field exposure systems are usually subject to field disturbances induced by external sources. Here, a method for designing a feedback control system for cancelling the effect of external ELF magnetic field disturbances on the magnetic field over the exposure area is presented. Th...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1997)18:4<299::aid-bem2>3.
更新日期:1997-01-01 00:00:00
abstract::Possible effects of 1439 MHz electromagnetic near field (EMF) exposure on the blood-brain barrier (BBB) were investigated using immature (4 weeks old) and young (10 weeks old) rats, equivalent in age to the time when the BBB development is completed and the young adult, respectively. Alteration of BBB related genes, s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20138
更新日期:2005-10-01 00:00:00
abstract::The use of wireless digital communication devices like GSM, WCDMA, HSPA, DECT, and WiFi changes the exposure of electromagnetic waves toward the user. Concentrating on the power variations on a slow and fast time scale, these new systems are discussed. Experimental results for both uplink and downlink are included for...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20569
更新日期:2010-05-01 00:00:00
abstract::Ozone can be produced by corona discharge either in dry air or when one electrode is submerged in water. Since ozone is toxic, we examined whether ozone production by corona near laboratory animals could reach levels of concern. Male rats were exposed to a corona discharge and the concentration of ozone produced was m...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10161
更新日期:2004-02-01 00:00:00
abstract::The potential ability of radiofrequency electromagnetic radiation (RFR) in the microwave range to induce mutagenesis, chromosomal aberrations, and sister chromatid exchanges in mammalian cells is being explored in our laboratories. In addition, we have also been examining the ability of simultaneous exposure to RFR an...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250110206
更新日期:1990-01-01 00:00:00
abstract::Electromagnetic sensibility refers to the ability to perceive the electromagnetic field (EMF) without necessarily developing health symptoms attributed to EMF exposure. A large sample of young healthy adults (n = 84) performed two forced-choice tasks on the perception of the GSM mobile phone EMF (902 MHz pulsed at 217...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20375
更新日期:2008-02-01 00:00:00
abstract::To be able to correlate real time membrane potential or ion flux changes with further downstream gene transcription responses due to extremely low frequency (ELF) electromagnetic field (EMF) exposure, we devised an experimental system consisting of a pair of symmetric circular coils. This system can be used on an inve...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10151
更新日期:2004-01-01 00:00:00
abstract::The treatment of long-term, stable para- and quadriplegics with pulsed electrical stimulation for pain control resulted in, anecdotally, a significant number of these individuals showing increased motor function as well as sensory awareness. This small pilot study was conducted in order to assess the hypothesis that p...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250080206
更新日期:1987-01-01 00:00:00
abstract::A new human head phantom has been proposed by CENELEC/IEEE, based on a large scale anthropometric survey. This phantom is compared to a homogeneous Generic Head Phantom and three high resolution anatomical head models with respect to specific absorption rate (SAR) assessment. The head phantoms are exposed to the radia...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20088
更新日期:2005-02-01 00:00:00
abstract::In epidemiological studies of electromagnetic fields and health effects, exposure classification is crucial. There is no generally accepted biophysical interaction mechanism, but many studies are based on the hypothesis of a causal relationship with the strength of magnetic field. Some definition of the magnitude of e...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(SICI)1521-186X(1996)17:3<209::AID-BEM6>3.
更新日期:1996-01-01 00:00:00
abstract::The effects of hyperthermia, alone and in conjunction with microwave exposure, on brain energetics were studied in anesthetized male Sprague-Dawley rats. The effect of temperature on adenosine triphosphate concentration [ATP] and creatine phosphate concentration [CP] was determined in the brains of rats that were main...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050107
更新日期:1984-01-01 00:00:00
abstract::In a previous study, we showed that operators of radiofrequency (RF) plastic sealers, RF operators (n = 35) had a lower heart rate during nighttime compared to a control group (n = 37). We have analyzed the heart rate variability (HRV) on the same group of people to better understand the possible underlying rhythm dis...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20276
更新日期:2007-01-01 00:00:00
abstract::The impedance spectroscopy technique (IST) was used for studying the effect of a 0.5 T magnetic field on the electrical properties of whole human blood. A Solartron SI 1260 spectrometer was used to measure the impedance spectra of magnetic field exposed blood samples compared to non-exposed samples. An equivalent elec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20132
更新日期:2005-10-01 00:00:00
abstract::As a result of ischaemia/reperfusion, massive generation of reactive oxygen species occurs, followed by decreased activity of antioxidant enzymes. Extremely low frequency electromagnetic fields (ELF-EMF) can modulate oxidative stress, but there are no clinical antioxidant studies in brain stroke patients. The aim of o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22055
更新日期:2017-07-01 00:00:00
abstract::Studies have evaluated the electroencephalography (EEG) of humans and laboratory animals during and after Radiofrequency (RF) exposures. Effects of RF exposure on the blood-brain barrier (BBB) have been generally accepted for exposures that are thermalizing. Low level exposures that report alterations of the BBB remai...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/bem.10179
更新日期:2003-01-01 00:00:00
abstract::Cell-based or magnetic field therapies as alternative approaches to pain management have been tested in several experimental pain models. The aim of this study therefore was to investigate the actions of the cell-based therapy (adipose tissue derived mesenchymal stem cells; ADMSC) or pulsed magnetic field (PMF) therap...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22038
更新日期:2017-05-01 00:00:00
abstract::Low-energy pulsed electromagnetic field (PEMF) therapy has been suggested as a promising therapy to increase microcirculation, which is of great concern in patients with fibromyalgia. This study evaluated the effectiveness of PEMF therapy on the treatment of fibromyalgia. A group of 108 women with fibromyalgia were al...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,随机对照试验
doi:10.1002/bem.22127
更新日期:2018-07-01 00:00:00
abstract::In studies investigating adverse reproductive outcomes associated with video display terminal (VDT) usage, amounts of time spent in front of a VDT or magnetic field (MF) levels in front of the VDT are used as surrogate measures of subject's MF exposure. However, the relevance of such surrogates to actual exposures has...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(SICI)1521-186X(1996)17:5<406::AID-BEM8>3.
更新日期:1996-01-01 00:00:00
abstract::The aim of this study was to elucidate the possible effects of short-term exposure to a 1439-MHz electromagnetic field (EMF) employing time division multiple access (TDMA), which is the basis of the Japanese Personal Digital Cellular system, on estrogenic activity in rats. Sixty-four ovariectomized female Sprague-Dawl...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20593
更新日期:2010-10-01 00:00:00
abstract::The present study examined the effect on rat cortical synaptosomes of a 2 h exposure to 50-Hz electromagnetic fields (EMFs) with a peak magnetic field of 2 mT. We measured modifications of synaptosomal mitochondrial respiration rate, ATP production, membrane potential, intrasynaptosomal Ca(2+) concentration and free i...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20322
更新日期:2007-09-01 00:00:00